Corynebacterium mustelae
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3046 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3GUR6|A0A0G3GUR6_9CORY Nicotinamide mononucleotide transporter PnuC OS=Corynebacterium mustelae OX=571915 GN=CMUST_02775 PE=3 SV=1
MM1 pKa = 7.1 TFDD4 pKa = 3.09 WQMPRR9 pKa = 11.84 DD10 pKa = 4.2 PFADD14 pKa = 4.14 DD15 pKa = 4.49 PNDD18 pKa = 3.64 PASFLEE24 pKa = 4.29 ADD26 pKa = 4.1 EE27 pKa = 4.76 PLPPLSDD34 pKa = 3.82 DD35 pKa = 3.52 EE36 pKa = 4.39 RR37 pKa = 11.84 AQIQQDD43 pKa = 3.99 LQLVAQFKK51 pKa = 10.47 NALQPRR57 pKa = 11.84 GINGIFFFCEE67 pKa = 3.79 DD68 pKa = 3.94 CEE70 pKa = 4.23 QQHH73 pKa = 6.32 FYY75 pKa = 11.26 DD76 pKa = 3.67 WDD78 pKa = 3.54 ILASNMLATLAGEE91 pKa = 4.35 LSPVHH96 pKa = 6.17 EE97 pKa = 4.66 PSVNPNPQAYY107 pKa = 9.46 VPWDD111 pKa = 3.43 YY112 pKa = 11.65 CLGYY116 pKa = 10.55 LDD118 pKa = 5.09 GLEE121 pKa = 4.15 APRR124 pKa = 5.31
Molecular weight: 14.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.846
Patrickios 0.896
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.75
Protein with the highest isoelectric point:
>tr|A0A0G3H403|A0A0G3H403_9CORY Acetylglutamate kinase OS=Corynebacterium mustelae OX=571915 GN=argB PE=3 SV=1
MM1 pKa = 7.46 TKK3 pKa = 10.27 GKK5 pKa = 8.73 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 10.01 KK18 pKa = 8.51 HH19 pKa = 4.28 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.72 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.749
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.486
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3046
0
3046
1001453
28
3035
328.8
35.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.967 ± 0.056
0.932 ± 0.015
5.808 ± 0.036
6.042 ± 0.047
3.677 ± 0.027
7.608 ± 0.051
2.317 ± 0.021
5.744 ± 0.034
3.604 ± 0.035
9.458 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.02
3.172 ± 0.028
5.108 ± 0.036
3.46 ± 0.025
5.668 ± 0.034
6.087 ± 0.033
6.422 ± 0.039
7.992 ± 0.041
1.474 ± 0.019
2.349 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here