Bacillus phage vB_BcoS-136

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BVR1|A0A3G3BVR1_9CAUD Uncharacterized protein OS=Bacillus phage vB_BcoS-136 OX=2419619 GN=vBBcoS136_00234 PE=4 SV=1
MM1 pKa = 7.4NKK3 pKa = 10.06SKK5 pKa = 9.93TEE7 pKa = 3.82ILSDD11 pKa = 3.51MLFGDD16 pKa = 4.77DD17 pKa = 5.03FSDD20 pKa = 4.79FIDD23 pKa = 3.41RR24 pKa = 11.84TGISSGQDD32 pKa = 2.83SYY34 pKa = 12.12GDD36 pKa = 3.57YY37 pKa = 11.22YY38 pKa = 10.52IIFDD42 pKa = 3.62THH44 pKa = 7.36EE45 pKa = 4.23EE46 pKa = 3.77MSEE49 pKa = 4.02FIRR52 pKa = 11.84LINEE56 pKa = 3.9KK57 pKa = 9.82TGSNFDD63 pKa = 3.06NDD65 pKa = 3.94YY66 pKa = 10.42EE67 pKa = 4.41VEE69 pKa = 4.14RR70 pKa = 11.84EE71 pKa = 3.65FDD73 pKa = 3.22IEE75 pKa = 4.43MVFSDD80 pKa = 5.04EE81 pKa = 4.12YY82 pKa = 10.39TLCSDD87 pKa = 3.27CHH89 pKa = 5.35EE90 pKa = 4.94VIRR93 pKa = 11.84TSPTGYY99 pKa = 9.67GWQPDD104 pKa = 4.45FYY106 pKa = 9.47MGDD109 pKa = 3.44GFIVCNKK116 pKa = 9.38CFNEE120 pKa = 4.14HH121 pKa = 6.09EE122 pKa = 4.96DD123 pKa = 3.8YY124 pKa = 11.11QEE126 pKa = 4.26SYY128 pKa = 11.38LLEE131 pKa = 4.87KK132 pKa = 10.51INNPMNAVNGLITEE146 pKa = 4.29KK147 pKa = 10.73QMEE150 pKa = 4.18EE151 pKa = 4.11LGFEE155 pKa = 4.3KK156 pKa = 10.94LNGDD160 pKa = 3.81SYY162 pKa = 11.8EE163 pKa = 4.55SGLHH167 pKa = 4.79SHH169 pKa = 5.55QTDD172 pKa = 3.41SPKK175 pKa = 10.76EE176 pKa = 3.92IFNEE180 pKa = 3.91LSDD183 pKa = 3.57KK184 pKa = 10.78HH185 pKa = 8.43DD186 pKa = 3.31EE187 pKa = 4.26VVFYY191 pKa = 10.85VDD193 pKa = 4.61SVGQFDD199 pKa = 3.81IDD201 pKa = 3.55FSVWVRR207 pKa = 3.43

Molecular weight: 24.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BVI1|A0A3G3BVI1_9CAUD Uncharacterized protein OS=Bacillus phage vB_BcoS-136 OX=2419619 GN=vBBcoS136_00151 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84NNNIASALQGMSEE16 pKa = 3.96AQARR20 pKa = 11.84RR21 pKa = 11.84VLQEE25 pKa = 3.69EE26 pKa = 4.51GRR28 pKa = 11.84KK29 pKa = 9.58LEE31 pKa = 4.58RR32 pKa = 11.84IARR35 pKa = 11.84SVWRR39 pKa = 11.84AYY41 pKa = 9.87LASYY45 pKa = 8.28TPKK48 pKa = 10.3EE49 pKa = 4.27YY50 pKa = 10.52ARR52 pKa = 11.84HH53 pKa = 5.7LGKK56 pKa = 9.93PSGVRR61 pKa = 11.84TGNSEE66 pKa = 3.84RR67 pKa = 11.84AIKK70 pKa = 10.2LKK72 pKa = 8.88QVKK75 pKa = 8.43RR76 pKa = 11.84TGINEE81 pKa = 4.03FGIEE85 pKa = 4.11LTWEE89 pKa = 3.98NDD91 pKa = 3.3LVYY94 pKa = 10.38HH95 pKa = 6.79DD96 pKa = 5.17SVMNHH101 pKa = 5.93KK102 pKa = 10.06KK103 pKa = 9.92GHH105 pKa = 5.32AVMLISSGWTSSRR118 pKa = 11.84GRR120 pKa = 11.84NKK122 pKa = 10.05DD123 pKa = 3.15VYY125 pKa = 10.8RR126 pKa = 11.84FGYY129 pKa = 10.54YY130 pKa = 10.07EE131 pKa = 4.34GFDD134 pKa = 3.55YY135 pKa = 10.97LGKK138 pKa = 10.36VEE140 pKa = 4.06KK141 pKa = 9.75TYY143 pKa = 11.5NAMKK147 pKa = 10.2HH148 pKa = 4.84PQVYY152 pKa = 10.55LEE154 pKa = 4.31IQWSGKK160 pKa = 7.14YY161 pKa = 10.15LKK163 pKa = 10.69

Molecular weight: 18.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

238

0

238

46297

32

2792

194.5

22.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.609 ± 0.239

0.801 ± 0.072

6.784 ± 0.124

8.726 ± 0.201

4.406 ± 0.141

5.979 ± 0.132

1.806 ± 0.084

7.959 ± 0.241

9.117 ± 0.196

8.184 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.106

6.318 ± 0.123

1.892 ± 0.101

2.892 ± 0.171

4.35 ± 0.126

5.979 ± 0.152

5.091 ± 0.191

6.566 ± 0.139

1.147 ± 0.075

4.434 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski