Amino acid dipepetide frequency for Bacillus phage vB_BcoS-136

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.367AlaCys: 0.367 ± 0.097
3.283AlaAsp: 3.283 ± 0.315
3.866AlaGlu: 3.866 ± 0.453
1.944AlaPhe: 1.944 ± 0.242
1.814AlaGly: 1.814 ± 0.234
0.734AlaHis: 0.734 ± 0.132
4.363AlaIle: 4.363 ± 0.291
4.406AlaLys: 4.406 ± 0.351
3.953AlaLeu: 3.953 ± 0.362
1.966AlaMet: 1.966 ± 0.254
3.305AlaAsn: 3.305 ± 0.276
0.67AlaPro: 0.67 ± 0.127
1.318AlaGln: 1.318 ± 0.17
2.225AlaArg: 2.225 ± 0.234
2.7AlaSer: 2.7 ± 0.309
3.175AlaThr: 3.175 ± 0.308
2.959AlaVal: 2.959 ± 0.273
0.778AlaTrp: 0.778 ± 0.135
2.268AlaTyr: 2.268 ± 0.216
0.0AlaXaa: 0.0 ± 0.0
Cys
0.238CysAla: 0.238 ± 0.076
0.151CysCys: 0.151 ± 0.052
0.734CysAsp: 0.734 ± 0.139
0.734CysGlu: 0.734 ± 0.158
0.216CysPhe: 0.216 ± 0.068
0.67CysGly: 0.67 ± 0.135
0.259CysHis: 0.259 ± 0.089
0.626CysIle: 0.626 ± 0.135
0.907CysLys: 0.907 ± 0.155
0.583CysLeu: 0.583 ± 0.117
0.13CysMet: 0.13 ± 0.051
0.454CysAsn: 0.454 ± 0.085
0.281CysPro: 0.281 ± 0.078
0.194CysGln: 0.194 ± 0.062
0.216CysArg: 0.216 ± 0.06
0.324CysSer: 0.324 ± 0.089
0.302CysThr: 0.302 ± 0.093
0.497CysVal: 0.497 ± 0.1
0.022CysTrp: 0.022 ± 0.021
0.475CysTyr: 0.475 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
3.024AspAla: 3.024 ± 0.338
0.691AspCys: 0.691 ± 0.12
4.147AspAsp: 4.147 ± 0.306
6.307AspGlu: 6.307 ± 0.37
3.888AspPhe: 3.888 ± 0.285
5.292AspGly: 5.292 ± 0.394
0.518AspHis: 0.518 ± 0.1
6.07AspIle: 6.07 ± 0.341
6.026AspLys: 6.026 ± 0.507
5.897AspLeu: 5.897 ± 0.37
2.398AspMet: 2.398 ± 0.215
4.104AspAsn: 4.104 ± 0.233
0.67AspPro: 0.67 ± 0.134
0.886AspGln: 0.886 ± 0.131
2.419AspArg: 2.419 ± 0.254
3.694AspSer: 3.694 ± 0.286
2.808AspThr: 2.808 ± 0.253
4.342AspVal: 4.342 ± 0.319
0.907AspTrp: 0.907 ± 0.138
3.758AspTyr: 3.758 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
4.298GluAla: 4.298 ± 0.41
0.626GluCys: 0.626 ± 0.144
5.508GluAsp: 5.508 ± 0.428
8.683GluGlu: 8.683 ± 0.626
3.089GluPhe: 3.089 ± 0.281
4.234GluGly: 4.234 ± 0.279
1.361GluHis: 1.361 ± 0.16
7.711GluIle: 7.711 ± 0.497
8.489GluLys: 8.489 ± 0.472
7.582GluLeu: 7.582 ± 0.447
3.046GluMet: 3.046 ± 0.268
5.659GluAsn: 5.659 ± 0.336
1.318GluPro: 1.318 ± 0.261
3.305GluGln: 3.305 ± 0.337
3.888GluArg: 3.888 ± 0.364
4.234GluSer: 4.234 ± 0.332
3.629GluThr: 3.629 ± 0.293
5.983GluVal: 5.983 ± 0.426
0.95GluTrp: 0.95 ± 0.167
3.65GluTyr: 3.65 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
1.598PheAla: 1.598 ± 0.196
0.259PheCys: 0.259 ± 0.069
3.434PheAsp: 3.434 ± 0.317
3.694PheGlu: 3.694 ± 0.263
1.663PhePhe: 1.663 ± 0.189
3.434PheGly: 3.434 ± 0.285
0.886PheHis: 0.886 ± 0.148
3.348PheIle: 3.348 ± 0.396
3.586PheLys: 3.586 ± 0.278
2.873PheLeu: 2.873 ± 0.275
1.274PheMet: 1.274 ± 0.202
3.305PheAsn: 3.305 ± 0.238
0.886PhePro: 0.886 ± 0.144
0.864PheGln: 0.864 ± 0.139
1.966PheArg: 1.966 ± 0.209
3.002PheSer: 3.002 ± 0.28
2.225PheThr: 2.225 ± 0.178
3.154PheVal: 3.154 ± 0.24
0.497PheTrp: 0.497 ± 0.104
2.117PheTyr: 2.117 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
2.7GlyAla: 2.7 ± 0.217
0.54GlyCys: 0.54 ± 0.117
3.542GlyAsp: 3.542 ± 0.303
4.687GlyGlu: 4.687 ± 0.323
3.175GlyPhe: 3.175 ± 0.269
3.478GlyGly: 3.478 ± 0.249
1.015GlyHis: 1.015 ± 0.157
4.882GlyIle: 4.882 ± 0.321
5.724GlyLys: 5.724 ± 0.43
4.601GlyLeu: 4.601 ± 0.31
1.858GlyMet: 1.858 ± 0.175
3.758GlyAsn: 3.758 ± 0.276
0.0GlyPro: 0.0 ± 0.0
1.685GlyGln: 1.685 ± 0.196
2.678GlyArg: 2.678 ± 0.239
3.37GlySer: 3.37 ± 0.349
3.37GlyThr: 3.37 ± 0.32
4.558GlyVal: 4.558 ± 0.326
1.037GlyTrp: 1.037 ± 0.164
3.132GlyTyr: 3.132 ± 0.272
0.0GlyXaa: 0.0 ± 0.0
His
0.994HisAla: 0.994 ± 0.158
0.194HisCys: 0.194 ± 0.072
1.274HisAsp: 1.274 ± 0.163
1.685HisGlu: 1.685 ± 0.207
0.95HisPhe: 0.95 ± 0.152
1.166HisGly: 1.166 ± 0.153
0.367HisHis: 0.367 ± 0.085
1.274HisIle: 1.274 ± 0.175
1.361HisLys: 1.361 ± 0.164
1.663HisLeu: 1.663 ± 0.223
0.346HisMet: 0.346 ± 0.089
1.102HisAsn: 1.102 ± 0.108
0.367HisPro: 0.367 ± 0.085
0.281HisGln: 0.281 ± 0.077
0.778HisArg: 0.778 ± 0.141
1.08HisSer: 1.08 ± 0.196
1.123HisThr: 1.123 ± 0.147
0.994HisVal: 0.994 ± 0.142
0.151HisTrp: 0.151 ± 0.067
0.907HisTyr: 0.907 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
4.342IleAla: 4.342 ± 0.271
0.562IleCys: 0.562 ± 0.103
6.782IleAsp: 6.782 ± 0.42
7.69IleGlu: 7.69 ± 0.434
3.11IlePhe: 3.11 ± 0.26
4.579IleGly: 4.579 ± 0.32
1.793IleHis: 1.793 ± 0.208
6.134IleIle: 6.134 ± 0.48
6.718IleLys: 6.718 ± 0.434
5.789IleLeu: 5.789 ± 0.399
1.836IleMet: 1.836 ± 0.206
4.838IleAsn: 4.838 ± 0.363
1.858IlePro: 1.858 ± 0.203
2.246IleGln: 2.246 ± 0.237
3.434IleArg: 3.434 ± 0.269
4.774IleSer: 4.774 ± 0.331
4.45IleThr: 4.45 ± 0.334
5.076IleVal: 5.076 ± 0.401
0.713IleTrp: 0.713 ± 0.133
2.873IleTyr: 2.873 ± 0.28
0.0IleXaa: 0.0 ± 0.0
Lys
4.558LysAla: 4.558 ± 0.419
0.605LysCys: 0.605 ± 0.131
6.588LysAsp: 6.588 ± 0.44
8.273LysGlu: 8.273 ± 0.55
3.434LysPhe: 3.434 ± 0.336
5.594LysGly: 5.594 ± 0.378
1.814LysHis: 1.814 ± 0.207
6.804LysIle: 6.804 ± 0.366
7.582LysLys: 7.582 ± 0.592
7.582LysLeu: 7.582 ± 0.338
2.657LysMet: 2.657 ± 0.273
5.551LysAsn: 5.551 ± 0.387
1.944LysPro: 1.944 ± 0.211
3.024LysGln: 3.024 ± 0.296
3.694LysArg: 3.694 ± 0.253
5.422LysSer: 5.422 ± 0.301
4.903LysThr: 4.903 ± 0.39
6.35LysVal: 6.35 ± 0.376
1.037LysTrp: 1.037 ± 0.145
3.758LysTyr: 3.758 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
3.931LeuAla: 3.931 ± 0.402
0.497LeuCys: 0.497 ± 0.103
5.443LeuAsp: 5.443 ± 0.323
8.035LeuGlu: 8.035 ± 0.421
4.082LeuPhe: 4.082 ± 0.326
4.298LeuGly: 4.298 ± 0.298
1.534LeuHis: 1.534 ± 0.18
5.162LeuIle: 5.162 ± 0.405
7.409LeuLys: 7.409 ± 0.418
7.085LeuLeu: 7.085 ± 0.437
2.052LeuMet: 2.052 ± 0.221
5.184LeuAsn: 5.184 ± 0.416
2.246LeuPro: 2.246 ± 0.216
2.786LeuGln: 2.786 ± 0.234
4.061LeuArg: 4.061 ± 0.346
4.946LeuSer: 4.946 ± 0.321
4.19LeuThr: 4.19 ± 0.357
5.292LeuVal: 5.292 ± 0.339
0.778LeuTrp: 0.778 ± 0.139
2.83LeuTyr: 2.83 ± 0.305
0.0LeuXaa: 0.0 ± 0.0
Met
1.728MetAla: 1.728 ± 0.267
0.324MetCys: 0.324 ± 0.089
1.642MetAsp: 1.642 ± 0.184
2.29MetGlu: 2.29 ± 0.198
1.555MetPhe: 1.555 ± 0.214
1.447MetGly: 1.447 ± 0.158
0.518MetHis: 0.518 ± 0.111
2.527MetIle: 2.527 ± 0.274
3.737MetLys: 3.737 ± 0.253
2.29MetLeu: 2.29 ± 0.203
0.821MetMet: 0.821 ± 0.145
1.944MetAsn: 1.944 ± 0.21
0.583MetPro: 0.583 ± 0.105
0.778MetGln: 0.778 ± 0.126
0.994MetArg: 0.994 ± 0.162
2.29MetSer: 2.29 ± 0.274
1.404MetThr: 1.404 ± 0.169
1.469MetVal: 1.469 ± 0.178
0.151MetTrp: 0.151 ± 0.066
1.08MetTyr: 1.08 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
3.11AsnAla: 3.11 ± 0.31
0.475AsnCys: 0.475 ± 0.122
3.672AsnAsp: 3.672 ± 0.264
5.292AsnGlu: 5.292 ± 0.321
2.376AsnPhe: 2.376 ± 0.19
4.73AsnGly: 4.73 ± 0.347
1.339AsnHis: 1.339 ± 0.164
5.249AsnIle: 5.249 ± 0.32
5.486AsnLys: 5.486 ± 0.335
4.86AsnLeu: 4.86 ± 0.386
1.836AsnMet: 1.836 ± 0.174
4.601AsnAsn: 4.601 ± 0.318
1.814AsnPro: 1.814 ± 0.216
1.598AsnGln: 1.598 ± 0.217
3.348AsnArg: 3.348 ± 0.293
3.564AsnSer: 3.564 ± 0.3
3.024AsnThr: 3.024 ± 0.241
3.65AsnVal: 3.65 ± 0.239
0.518AsnTrp: 0.518 ± 0.115
2.635AsnTyr: 2.635 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
0.497ProAla: 0.497 ± 0.098
0.194ProCys: 0.194 ± 0.08
1.015ProAsp: 1.015 ± 0.16
1.534ProGlu: 1.534 ± 0.235
0.95ProPhe: 0.95 ± 0.172
0.0ProGly: 0.0 ± 0.0
0.346ProHis: 0.346 ± 0.082
1.447ProIle: 1.447 ± 0.177
1.836ProLys: 1.836 ± 0.217
1.577ProLeu: 1.577 ± 0.228
0.454ProMet: 0.454 ± 0.113
1.404ProAsn: 1.404 ± 0.194
0.367ProPro: 0.367 ± 0.105
0.713ProGln: 0.713 ± 0.124
0.713ProArg: 0.713 ± 0.133
1.75ProSer: 1.75 ± 0.202
1.426ProThr: 1.426 ± 0.177
1.49ProVal: 1.49 ± 0.174
0.259ProTrp: 0.259 ± 0.08
0.95ProTyr: 0.95 ± 0.168
0.0ProXaa: 0.0 ± 0.0
Gln
1.75GlnAla: 1.75 ± 0.284
0.194GlnCys: 0.194 ± 0.067
1.642GlnAsp: 1.642 ± 0.192
2.57GlnGlu: 2.57 ± 0.228
1.102GlnPhe: 1.102 ± 0.132
1.577GlnGly: 1.577 ± 0.18
0.54GlnHis: 0.54 ± 0.121
2.16GlnIle: 2.16 ± 0.223
2.506GlnLys: 2.506 ± 0.251
3.11GlnLeu: 3.11 ± 0.365
0.864GlnMet: 0.864 ± 0.152
1.469GlnAsn: 1.469 ± 0.18
0.583GlnPro: 0.583 ± 0.103
1.21GlnGln: 1.21 ± 0.286
1.447GlnArg: 1.447 ± 0.202
1.49GlnSer: 1.49 ± 0.145
1.166GlnThr: 1.166 ± 0.148
1.836GlnVal: 1.836 ± 0.22
0.475GlnTrp: 0.475 ± 0.096
1.231GlnTyr: 1.231 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
1.987ArgAla: 1.987 ± 0.257
0.346ArgCys: 0.346 ± 0.084
2.635ArgAsp: 2.635 ± 0.258
4.169ArgGlu: 4.169 ± 0.4
1.966ArgPhe: 1.966 ± 0.205
2.657ArgGly: 2.657 ± 0.306
0.713ArgHis: 0.713 ± 0.114
3.24ArgIle: 3.24 ± 0.241
4.622ArgLys: 4.622 ± 0.367
3.78ArgLeu: 3.78 ± 0.308
1.512ArgMet: 1.512 ± 0.153
2.549ArgAsn: 2.549 ± 0.257
0.54ArgPro: 0.54 ± 0.103
1.75ArgGln: 1.75 ± 0.263
1.728ArgArg: 1.728 ± 0.272
2.095ArgSer: 2.095 ± 0.177
1.901ArgThr: 1.901 ± 0.215
2.657ArgVal: 2.657 ± 0.229
0.67ArgTrp: 0.67 ± 0.133
1.987ArgTyr: 1.987 ± 0.178
0.0ArgXaa: 0.0 ± 0.0
Ser
2.938SerAla: 2.938 ± 0.297
0.389SerCys: 0.389 ± 0.096
4.32SerAsp: 4.32 ± 0.306
4.234SerGlu: 4.234 ± 0.32
2.765SerPhe: 2.765 ± 0.244
3.413SerGly: 3.413 ± 0.276
1.123SerHis: 1.123 ± 0.155
4.752SerIle: 4.752 ± 0.389
5.486SerLys: 5.486 ± 0.413
5.53SerLeu: 5.53 ± 0.306
1.685SerMet: 1.685 ± 0.196
3.672SerAsn: 3.672 ± 0.309
1.231SerPro: 1.231 ± 0.191
1.858SerGln: 1.858 ± 0.213
2.549SerArg: 2.549 ± 0.191
4.082SerSer: 4.082 ± 0.407
2.851SerThr: 2.851 ± 0.279
3.542SerVal: 3.542 ± 0.261
0.67SerTrp: 0.67 ± 0.112
2.7SerTyr: 2.7 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
2.549ThrAla: 2.549 ± 0.321
0.475ThrCys: 0.475 ± 0.118
3.11ThrAsp: 3.11 ± 0.311
3.434ThrGlu: 3.434 ± 0.313
2.527ThrPhe: 2.527 ± 0.21
3.434ThrGly: 3.434 ± 0.306
0.799ThrHis: 0.799 ± 0.113
4.601ThrIle: 4.601 ± 0.317
3.65ThrLys: 3.65 ± 0.236
4.212ThrLeu: 4.212 ± 0.307
1.534ThrMet: 1.534 ± 0.175
3.132ThrAsn: 3.132 ± 0.308
1.512ThrPro: 1.512 ± 0.21
1.469ThrGln: 1.469 ± 0.171
2.095ThrArg: 2.095 ± 0.244
3.024ThrSer: 3.024 ± 0.328
3.067ThrThr: 3.067 ± 0.303
3.197ThrVal: 3.197 ± 0.242
0.562ThrTrp: 0.562 ± 0.109
2.527ThrTyr: 2.527 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
3.456ValAla: 3.456 ± 0.258
0.518ValCys: 0.518 ± 0.139
4.99ValAsp: 4.99 ± 0.386
5.746ValGlu: 5.746 ± 0.406
2.959ValPhe: 2.959 ± 0.29
4.298ValGly: 4.298 ± 0.298
1.08ValHis: 1.08 ± 0.178
4.774ValIle: 4.774 ± 0.404
6.458ValLys: 6.458 ± 0.378
4.579ValLeu: 4.579 ± 0.32
1.706ValMet: 1.706 ± 0.211
3.974ValAsn: 3.974 ± 0.308
1.015ValPro: 1.015 ± 0.141
1.339ValGln: 1.339 ± 0.174
2.851ValArg: 2.851 ± 0.281
4.32ValSer: 4.32 ± 0.258
2.808ValThr: 2.808 ± 0.248
5.162ValVal: 5.162 ± 0.459
0.799ValTrp: 0.799 ± 0.15
2.83ValTyr: 2.83 ± 0.3
0.0ValXaa: 0.0 ± 0.0
Trp
0.432TrpAla: 0.432 ± 0.084
0.173TrpCys: 0.173 ± 0.057
0.842TrpAsp: 0.842 ± 0.131
0.886TrpGlu: 0.886 ± 0.135
0.497TrpPhe: 0.497 ± 0.105
0.605TrpGly: 0.605 ± 0.12
0.432TrpHis: 0.432 ± 0.104
0.799TrpIle: 0.799 ± 0.14
1.21TrpLys: 1.21 ± 0.204
0.842TrpLeu: 0.842 ± 0.124
0.389TrpMet: 0.389 ± 0.081
0.691TrpAsn: 0.691 ± 0.121
0.0TrpPro: 0.0 ± 0.0
0.281TrpGln: 0.281 ± 0.084
0.562TrpArg: 0.562 ± 0.114
0.734TrpSer: 0.734 ± 0.129
0.562TrpThr: 0.562 ± 0.117
0.886TrpVal: 0.886 ± 0.145
0.173TrpTrp: 0.173 ± 0.068
0.475TrpTyr: 0.475 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.966TyrAla: 1.966 ± 0.234
0.432TyrCys: 0.432 ± 0.102
3.24TyrAsp: 3.24 ± 0.254
3.456TyrGlu: 3.456 ± 0.305
1.814TyrPhe: 1.814 ± 0.215
3.067TyrGly: 3.067 ± 0.227
0.886TyrHis: 0.886 ± 0.132
3.672TyrIle: 3.672 ± 0.341
3.974TyrLys: 3.974 ± 0.292
3.456TyrLeu: 3.456 ± 0.303
1.188TyrMet: 1.188 ± 0.151
2.484TyrAsn: 2.484 ± 0.219
1.037TyrPro: 1.037 ± 0.188
1.339TyrGln: 1.339 ± 0.161
1.858TyrArg: 1.858 ± 0.185
2.894TyrSer: 2.894 ± 0.259
2.527TyrThr: 2.527 ± 0.24
2.57TyrVal: 2.57 ± 0.248
0.324TyrTrp: 0.324 ± 0.092
2.16TyrTyr: 2.16 ± 0.259
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 238 proteins (46297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski