Xanthomonas phage Xoo-sp15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Caeruleovirus; unclassified Caeruleovirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7M732|A0A6B7M732_9CAUD Uncharacterized protein OS=Xanthomonas phage Xoo-sp15 OX=2608370 GN=Xoosp15_40 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.04MPWEE6 pKa = 4.75CEE8 pKa = 3.99DD9 pKa = 4.32CGHH12 pKa = 6.98KK13 pKa = 10.38EE14 pKa = 3.65LASPFDD20 pKa = 3.77TFMLCPNCDD29 pKa = 3.22SQNFFHH35 pKa = 7.24GSIIEE40 pKa = 4.42DD41 pKa = 3.48DD42 pKa = 3.77VEE44 pKa = 4.14EE45 pKa = 4.07YY46 pKa = 11.0NIYY49 pKa = 10.98DD50 pKa = 4.51EE51 pKa = 6.83DD52 pKa = 5.69DD53 pKa = 4.4DD54 pKa = 5.48LDD56 pKa = 3.86NVTVFEE62 pKa = 4.5DD63 pKa = 3.45QFGEE67 pKa = 4.0KK68 pKa = 9.13LTIVNEE74 pKa = 3.86IDD76 pKa = 4.1DD77 pKa = 5.18DD78 pKa = 5.13GDD80 pKa = 3.98INLLPSDD87 pKa = 3.87RR88 pKa = 11.84NLFFSRR94 pKa = 11.84EE95 pKa = 3.89DD96 pKa = 3.79AIEE99 pKa = 3.94LAKK102 pKa = 10.81CILKK106 pKa = 10.24VCGEE110 pKa = 4.18EE111 pKa = 4.4VEE113 pKa = 4.33

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B7MBK0|A0A6B7MBK0_9CAUD Uncharacterized protein OS=Xanthomonas phage Xoo-sp15 OX=2608370 GN=Xoosp15_173 PE=4 SV=1
MM1 pKa = 6.78VRR3 pKa = 11.84KK4 pKa = 9.56ARR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84LFKK12 pKa = 11.03SNNSIKK18 pKa = 9.85TAMTNLGDD26 pKa = 4.49NLAQDD31 pKa = 3.98VLDD34 pKa = 3.55TGMRR38 pKa = 11.84TIIASKK44 pKa = 10.3PKK46 pKa = 10.21DD47 pKa = 3.22ISAKK51 pKa = 10.31RR52 pKa = 11.84MPKK55 pKa = 9.51YY56 pKa = 10.68LKK58 pKa = 9.55LTEE61 pKa = 3.97EE62 pKa = 4.21RR63 pKa = 11.84LEE65 pKa = 3.99KK66 pKa = 11.2LEE68 pKa = 4.7VIDD71 pKa = 4.97LKK73 pKa = 11.19PYY75 pKa = 9.1FAKK78 pKa = 10.44SSKK81 pKa = 10.49RR82 pKa = 11.84KK83 pKa = 6.95TKK85 pKa = 10.51KK86 pKa = 10.33DD87 pKa = 3.25GGWYY91 pKa = 8.25LTVPIRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 9.74ARR101 pKa = 11.84GMSRR105 pKa = 11.84RR106 pKa = 11.84MYY108 pKa = 8.73EE109 pKa = 3.45QLRR112 pKa = 11.84AVDD115 pKa = 3.97IGDD118 pKa = 4.14SEE120 pKa = 4.99SKK122 pKa = 9.82TVVSDD127 pKa = 3.2YY128 pKa = 11.0LYY130 pKa = 10.91DD131 pKa = 3.92RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84QSDD137 pKa = 3.58ASLLNYY143 pKa = 8.06TPKK146 pKa = 10.8SNNINKK152 pKa = 8.56MKK154 pKa = 10.38AGRR157 pKa = 11.84NRR159 pKa = 11.84HH160 pKa = 6.4DD161 pKa = 3.33YY162 pKa = 10.11VAFRR166 pKa = 11.84TVSDD170 pKa = 3.95KK171 pKa = 11.52SPAGSWIINRR181 pKa = 11.84DD182 pKa = 3.46KK183 pKa = 11.42VNKK186 pKa = 10.01DD187 pKa = 3.22DD188 pKa = 3.69TSKK191 pKa = 9.88TFVANVNRR199 pKa = 11.84LMKK202 pKa = 9.97WKK204 pKa = 9.06MKK206 pKa = 10.98NGMM209 pKa = 4.0

Molecular weight: 24.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

46547

38

1280

203.3

23.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.947 ± 0.195

0.857 ± 0.073

6.421 ± 0.129

7.927 ± 0.288

3.863 ± 0.104

6.671 ± 0.25

1.792 ± 0.101

6.355 ± 0.128

7.829 ± 0.184

8.005 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.086

5.319 ± 0.158

3.134 ± 0.153

3.672 ± 0.139

4.361 ± 0.119

5.856 ± 0.159

6.428 ± 0.189

7.231 ± 0.17

1.19 ± 0.071

4.393 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski