Arthrobacter phage DrSierra

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Manhattanvirus; unclassified Manhattanvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XKZ4|A0A6G6XKZ4_9CAUD Uncharacterized protein OS=Arthrobacter phage DrSierra OX=2704034 GN=47 PE=4 SV=1
MM1 pKa = 7.88IDD3 pKa = 4.75LEE5 pKa = 5.31DD6 pKa = 5.05DD7 pKa = 3.36VDD9 pKa = 4.65LEE11 pKa = 4.26LDD13 pKa = 3.26EE14 pKa = 5.78RR15 pKa = 11.84EE16 pKa = 4.53YY17 pKa = 11.5VCEE20 pKa = 4.16TCRR23 pKa = 11.84LTHH26 pKa = 6.06WRR28 pKa = 11.84GAPDD32 pKa = 3.65PCDD35 pKa = 3.41RR36 pKa = 11.84SS37 pKa = 3.51

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XKF2|A0A6G6XKF2_9CAUD Tape measure protein OS=Arthrobacter phage DrSierra OX=2704034 GN=16 PE=4 SV=1
MM1 pKa = 7.24IHH3 pKa = 7.03GNRR6 pKa = 11.84DD7 pKa = 3.49DD8 pKa = 4.92LLWLAGLLEE17 pKa = 4.55GEE19 pKa = 4.75GTFDD23 pKa = 3.26AHH25 pKa = 6.21RR26 pKa = 11.84GKK28 pKa = 10.74YY29 pKa = 9.13PRR31 pKa = 11.84IRR33 pKa = 11.84LAMTDD38 pKa = 3.13RR39 pKa = 11.84DD40 pKa = 4.23VVGRR44 pKa = 11.84AASLMDD50 pKa = 3.63SKK52 pKa = 10.94IRR54 pKa = 11.84LSLHH58 pKa = 6.16PAPAQATWHH67 pKa = 6.36TEE69 pKa = 3.65LSGEE73 pKa = 3.99KK74 pKa = 9.58AAAIMRR80 pKa = 11.84EE81 pKa = 3.98ILPFMGTRR89 pKa = 11.84RR90 pKa = 11.84SGKK93 pKa = 8.35IANVLGVAHH102 pKa = 6.63FRR104 pKa = 11.84QRR106 pKa = 11.84AVVDD110 pKa = 3.88AGTGSTPGPRR120 pKa = 11.84VVRR123 pKa = 11.84PAGVAKK129 pKa = 10.31PLTAAA134 pKa = 4.22

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13128

36

831

198.9

21.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.698 ± 0.351

0.617 ± 0.126

6.498 ± 0.349

6.688 ± 0.379

3.275 ± 0.159

8.478 ± 0.448

1.92 ± 0.176

4.136 ± 0.256

4.106 ± 0.311

8.828 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.156

2.658 ± 0.199

5.477 ± 0.339

2.262 ± 0.213

7.366 ± 0.489

5.309 ± 0.262

6.345 ± 0.398

7.191 ± 0.301

1.737 ± 0.13

2.529 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski