Sphingomonas taxi
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3401 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097EFW2|A0A097EFW2_9SPHN Amidophosphoribosyltransferase OS=Sphingomonas taxi OX=1549858 GN=MC45_08720 PE=4 SV=1
MM1 pKa = 7.27 LVIAEE6 pKa = 4.57 ADD8 pKa = 3.52 GGDD11 pKa = 3.69 DD12 pKa = 3.18 VGAGVGAGVGDD23 pKa = 3.95 GVGAGAGVGVTIGVGVGAGVCAVGGAGTLFTPPPPPPPQAARR65 pKa = 11.84 NSGAATAAPPVNQVFVVITCPPP87 pKa = 3.44
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.876
IPC2_protein 3.706
IPC_protein 3.452
Toseland 3.3
ProMoST 3.745
Dawson 3.516
Bjellqvist 3.706
Wikipedia 3.579
Rodwell 3.338
Grimsley 3.249
Solomon 3.414
Lehninger 3.363
Nozaki 3.719
DTASelect 3.872
Thurlkill 3.439
EMBOSS 3.579
Sillero 3.617
Patrickios 1.863
IPC_peptide 3.401
IPC2_peptide 3.554
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|A0A097ECT1|A0A097ECT1_9SPHN 30S ribosomal protein S20 OS=Sphingomonas taxi OX=1549858 GN=rpsT PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 AVIHH32 pKa = 6.17 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.618
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.34
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3401
0
3401
1123099
29
2825
330.2
35.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.436 ± 0.071
0.665 ± 0.012
6.166 ± 0.03
4.688 ± 0.04
3.376 ± 0.025
9.15 ± 0.04
1.96 ± 0.021
4.733 ± 0.026
2.524 ± 0.032
9.82 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.173 ± 0.021
2.389 ± 0.036
5.475 ± 0.035
3.04 ± 0.028
7.637 ± 0.042
4.735 ± 0.037
5.794 ± 0.04
7.535 ± 0.032
1.426 ± 0.02
2.279 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here