Plantibacter sp. Leaf314

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Plantibacter; unclassified Plantibacter

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5EA72|A0A0Q5EA72_9MICO GTPase Der OS=Plantibacter sp. Leaf314 OX=1736333 GN=der PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.41RR3 pKa = 11.84STLPALAVVLLALAGCSAGTPTQTSAPSATTAAPTATPSATSEE46 pKa = 4.05PMSDD50 pKa = 3.59ASCEE54 pKa = 4.07TVLTDD59 pKa = 4.35EE60 pKa = 5.34EE61 pKa = 4.71YY62 pKa = 11.23ASLAEE67 pKa = 5.24DD68 pKa = 4.52GLVLDD73 pKa = 4.84PDD75 pKa = 3.87IFVLDD80 pKa = 4.24DD81 pKa = 3.54TMQSIIDD88 pKa = 3.78AGGLGCYY95 pKa = 7.46WARR98 pKa = 11.84SGGDD102 pKa = 2.83IRR104 pKa = 11.84VWYY107 pKa = 9.43AQGAKK112 pKa = 9.32TSAEE116 pKa = 3.82WDD118 pKa = 3.66TQKK121 pKa = 10.88QSLIGAGWTEE131 pKa = 3.77TDD133 pKa = 3.33ASVAGALQAPDD144 pKa = 4.34VNDD147 pKa = 4.1PDD149 pKa = 4.01YY150 pKa = 11.39LPVVTYY156 pKa = 10.6RR157 pKa = 11.84DD158 pKa = 3.33GVVYY162 pKa = 9.81YY163 pKa = 10.92ASVANLLPSVKK174 pKa = 10.17ALQEE178 pKa = 3.89

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5EMG7|A0A0Q5EMG7_9MICO FAD-dependent oxidoreductase OS=Plantibacter sp. Leaf314 OX=1736333 GN=ASF68_12795 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3521

0

3521

1149947

29

3228

326.6

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.296 ± 0.055

0.458 ± 0.009

6.29 ± 0.034

5.638 ± 0.037

3.174 ± 0.023

8.944 ± 0.035

1.947 ± 0.021

4.518 ± 0.031

1.827 ± 0.028

10.33 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.662 ± 0.017

1.87 ± 0.018

5.388 ± 0.03

2.822 ± 0.025

7.124 ± 0.047

5.825 ± 0.027

6.363 ± 0.038

9.156 ± 0.043

1.46 ± 0.021

1.906 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski