Vagococcus teuberi
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1935 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J0A777|A0A1J0A777_9ENTE Bacteriocin ABC transporter ATP-binding protein OS=Vagococcus teuberi OX=519472 GN=BHY08_08060 PE=4 SV=1
MM1 pKa = 7.85 AIAKK5 pKa = 9.25 IVYY8 pKa = 10.22 ASLTGNTEE16 pKa = 3.93 EE17 pKa = 4.62 IADD20 pKa = 3.63 IVAEE24 pKa = 3.94 RR25 pKa = 11.84 LEE27 pKa = 4.32 EE28 pKa = 4.33 KK29 pKa = 10.86 GLDD32 pKa = 3.74 VEE34 pKa = 5.57 IEE36 pKa = 3.98 EE37 pKa = 4.6 CTQVDD42 pKa = 3.46 AEE44 pKa = 4.56 EE45 pKa = 4.85 FLDD48 pKa = 4.67 ADD50 pKa = 3.47 ICIVGSYY57 pKa = 9.76 TYY59 pKa = 11.3 DD60 pKa = 3.42 DD61 pKa = 3.78 VLVPDD66 pKa = 5.61 EE67 pKa = 4.54 IADD70 pKa = 4.14 FYY72 pKa = 11.72 EE73 pKa = 5.4 DD74 pKa = 5.31 LLEE77 pKa = 5.62 LDD79 pKa = 4.43 LTGKK83 pKa = 10.09 IYY85 pKa = 10.66 GVIGSGDD92 pKa = 3.13 TFYY95 pKa = 10.87 PCFCQVVDD103 pKa = 3.95 QFDD106 pKa = 3.58 KK107 pKa = 11.3 AFQSTGAIKK116 pKa = 10.27 GAEE119 pKa = 4.13 SVKK122 pKa = 10.33 VDD124 pKa = 4.23 LAAEE128 pKa = 4.15 EE129 pKa = 4.35 DD130 pKa = 4.51 DD131 pKa = 4.4 IVALEE136 pKa = 4.25 AFADD140 pKa = 3.95 ALVKK144 pKa = 10.74 QLDD147 pKa = 3.47
Molecular weight: 16.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.554
IPC_protein 3.554
Toseland 3.338
ProMoST 3.681
Dawson 3.541
Bjellqvist 3.732
Wikipedia 3.478
Rodwell 3.376
Grimsley 3.249
Solomon 3.528
Lehninger 3.478
Nozaki 3.656
DTASelect 3.872
Thurlkill 3.389
EMBOSS 3.478
Sillero 3.668
Patrickios 0.947
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|A0A1J0A8D0|A0A1J0A8D0_9ENTE Lysine--tRNA ligase OS=Vagococcus teuberi OX=519472 GN=lysS PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.7 QPKK8 pKa = 8.83 KK9 pKa = 9.05 RR10 pKa = 11.84 KK11 pKa = 7.43 RR12 pKa = 11.84 QKK14 pKa = 8.87 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.8 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLAAA44 pKa = 4.31
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.435
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.457
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.179
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1935
0
1935
603234
37
1783
311.7
35.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.06 ± 0.057
0.655 ± 0.014
5.814 ± 0.053
7.216 ± 0.063
4.44 ± 0.051
6.405 ± 0.061
1.859 ± 0.024
8.052 ± 0.059
7.432 ± 0.056
9.738 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.029
4.83 ± 0.05
3.33 ± 0.035
3.701 ± 0.04
3.467 ± 0.045
6.497 ± 0.051
6.019 ± 0.05
7.116 ± 0.052
0.819 ± 0.018
3.724 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here