Acanthochromis polyacanthus (spiny chromis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q1H447|A0A3Q1H447_9TELE Zinc finger protein 556-like OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1
TT1 pKa = 7.16IAEE4 pKa = 4.77CLPCGKK10 pKa = 7.74QHH12 pKa = 7.23KK13 pKa = 10.58NFDD16 pKa = 4.47LDD18 pKa = 3.66PAPLIKK24 pKa = 10.42CPPGTCFRR32 pKa = 11.84QNMCIPSNSGRR43 pKa = 11.84FQCAPCPDD51 pKa = 4.17GFTGDD56 pKa = 4.38GVHH59 pKa = 7.02CDD61 pKa = 5.04DD62 pKa = 5.32VDD64 pKa = 3.52EE65 pKa = 5.54CKK67 pKa = 10.62FNPCFPGVRR76 pKa = 11.84CVNTAPGFRR85 pKa = 11.84CEE87 pKa = 4.09KK88 pKa = 10.56CPLGYY93 pKa = 9.12TGPLLTGVGVSYY105 pKa = 11.38AKK107 pKa = 9.9TNKK110 pKa = 9.05QVCEE114 pKa = 5.17DD115 pKa = 3.46IDD117 pKa = 3.98EE118 pKa = 4.7CLGPPDD124 pKa = 5.25NGGCTANSDD133 pKa = 3.94CHH135 pKa = 5.11NTMGSFRR142 pKa = 11.84CGNCKK147 pKa = 10.33SGFTGDD153 pKa = 3.73QVRR156 pKa = 11.84GCHH159 pKa = 6.9GDD161 pKa = 3.47RR162 pKa = 11.84LCPNGQPNPCDD173 pKa = 3.35INAEE177 pKa = 4.41CIVEE181 pKa = 4.0RR182 pKa = 11.84DD183 pKa = 3.56GSISCACGVGWAGNGYY199 pKa = 9.96VCGKK203 pKa = 9.08DD204 pKa = 3.26TDD206 pKa = 4.13IDD208 pKa = 3.69AHH210 pKa = 6.92PDD212 pKa = 3.21DD213 pKa = 4.71TLQCRR218 pKa = 11.84DD219 pKa = 3.77STCKK223 pKa = 9.81QDD225 pKa = 3.25NCVFVPNSGQEE236 pKa = 3.9DD237 pKa = 4.01ADD239 pKa = 4.23RR240 pKa = 11.84DD241 pKa = 4.12GMGDD245 pKa = 3.44ACDD248 pKa = 5.25DD249 pKa = 4.49DD250 pKa = 6.68ADD252 pKa = 4.25SDD254 pKa = 4.63GIMNIDD260 pKa = 3.79DD261 pKa = 4.27NCWLVPNVNQKK272 pKa = 10.44NSDD275 pKa = 3.57KK276 pKa = 10.78DD277 pKa = 3.66LHH279 pKa = 7.57GDD281 pKa = 3.17ACDD284 pKa = 3.37NCKK287 pKa = 9.07TVEE290 pKa = 4.15NPSQRR295 pKa = 11.84DD296 pKa = 3.21TDD298 pKa = 3.54QDD300 pKa = 3.95GLGDD304 pKa = 3.9EE305 pKa = 5.58CDD307 pKa = 4.71DD308 pKa = 5.76DD309 pKa = 4.25MDD311 pKa = 6.43GDD313 pKa = 4.13GLKK316 pKa = 10.81NILDD320 pKa = 3.68NCQRR324 pKa = 11.84VPNLDD329 pKa = 3.29QKK331 pKa = 11.54DD332 pKa = 3.66RR333 pKa = 11.84DD334 pKa = 3.67NDD336 pKa = 4.11GVGDD340 pKa = 4.22ACDD343 pKa = 3.93SCPDD347 pKa = 3.54MANPNQSDD355 pKa = 3.51SDD357 pKa = 4.12DD358 pKa = 4.47DD359 pKa = 4.96LVGDD363 pKa = 4.17TCDD366 pKa = 5.48DD367 pKa = 4.73NIDD370 pKa = 3.69SDD372 pKa = 4.95GDD374 pKa = 3.6GHH376 pKa = 7.04QNTKK380 pKa = 10.88DD381 pKa = 3.38NCPDD385 pKa = 3.72VINSSQLDD393 pKa = 3.62TDD395 pKa = 3.7KK396 pKa = 11.69DD397 pKa = 4.0GMGDD401 pKa = 3.49EE402 pKa = 5.68CDD404 pKa = 5.62DD405 pKa = 5.68DD406 pKa = 6.32DD407 pKa = 7.09DD408 pKa = 6.04NDD410 pKa = 5.26GILDD414 pKa = 3.81EE415 pKa = 6.04DD416 pKa = 4.7DD417 pKa = 3.7NCRR420 pKa = 11.84LVPNPDD426 pKa = 3.22QEE428 pKa = 4.67DD429 pKa = 3.77ANKK432 pKa = 10.9DD433 pKa = 3.63KK434 pKa = 11.66VGDD437 pKa = 3.7ACEE440 pKa = 4.28GDD442 pKa = 3.48FDD444 pKa = 4.85KK445 pKa = 11.65DD446 pKa = 3.6NVIDD450 pKa = 5.66IIDD453 pKa = 4.07HH454 pKa = 6.03CPEE457 pKa = 3.9NAEE460 pKa = 4.03VTLTDD465 pKa = 3.81FRR467 pKa = 11.84AYY469 pKa = 8.19QTVVLDD475 pKa = 4.24PEE477 pKa = 5.01GDD479 pKa = 3.9SQIDD483 pKa = 3.89PNWVVLNQGMEE494 pKa = 4.11IVQTMNSDD502 pKa = 3.19PGLAVGYY509 pKa = 9.83KK510 pKa = 10.02AFSGVDD516 pKa = 3.74FEE518 pKa = 5.24GTFHH522 pKa = 6.81VNTVTDD528 pKa = 3.57DD529 pKa = 4.08DD530 pKa = 4.35YY531 pKa = 12.0AGFIFGYY538 pKa = 9.58QDD540 pKa = 2.83SSSFYY545 pKa = 10.47VVMWKK550 pKa = 7.78QTEE553 pKa = 3.84QTYY556 pKa = 8.18WQAAPFRR563 pKa = 11.84AVADD567 pKa = 3.86PGIQLKK573 pKa = 10.07AVKK576 pKa = 10.09SKK578 pKa = 9.5TGPGEE583 pKa = 3.83YY584 pKa = 10.03LRR586 pKa = 11.84NSLWHH591 pKa = 6.45TGNTPDD597 pKa = 5.06QVRR600 pKa = 11.84LLWKK604 pKa = 10.3DD605 pKa = 3.22PRR607 pKa = 11.84NVGWKK612 pKa = 10.39DD613 pKa = 3.18KK614 pKa = 11.21VSYY617 pKa = 10.33RR618 pKa = 11.84WFLQHH623 pKa = 6.81RR624 pKa = 11.84PQVGYY629 pKa = 9.82IRR631 pKa = 11.84ARR633 pKa = 11.84FFEE636 pKa = 4.37GSNLVADD643 pKa = 4.25TGMITDD649 pKa = 3.77TSMRR653 pKa = 11.84GGRR656 pKa = 11.84LGVFCFSQEE665 pKa = 3.94NIIWSNLKK673 pKa = 9.68YY674 pKa = 10.31RR675 pKa = 11.84CNDD678 pKa = 4.36TIPADD683 pKa = 3.95YY684 pKa = 10.4QDD686 pKa = 3.95QPTAQTEE693 pKa = 4.24LSDD696 pKa = 3.9GLL698 pKa = 4.0

Molecular weight: 76.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q1F122|A0A3Q1F122_9TELE DENN domain containing 2B OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1
MM1 pKa = 6.95FQSSHH6 pKa = 3.95KK7 pKa = 8.31TFRR10 pKa = 11.84IKK12 pKa = 10.64RR13 pKa = 11.84FLAKK17 pKa = 9.71KK18 pKa = 9.58QKK20 pKa = 8.69QNRR23 pKa = 11.84PIPQWIRR30 pKa = 11.84MKK32 pKa = 9.89TGNKK36 pKa = 8.61IRR38 pKa = 11.84YY39 pKa = 7.09NSKK42 pKa = 8.3RR43 pKa = 11.84RR44 pKa = 11.84HH45 pKa = 3.95WRR47 pKa = 11.84RR48 pKa = 11.84TKK50 pKa = 10.83LGLL53 pKa = 3.67

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23617

9863

33480

18433522

16

8635

550.6

61.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.528 ± 0.011

2.261 ± 0.011

5.22 ± 0.009

6.817 ± 0.018

3.636 ± 0.01

6.288 ± 0.015

2.67 ± 0.006

4.343 ± 0.01

5.603 ± 0.013

9.491 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.006

3.813 ± 0.008

5.743 ± 0.017

4.771 ± 0.012

5.665 ± 0.012

8.853 ± 0.019

5.636 ± 0.01

6.336 ± 0.011

1.144 ± 0.005

2.739 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski