Amino acid dipepetide frequency for Acanthochromis polyacanthus (spiny chromis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.363AlaAla: 6.363 ± 0.033	1.263AlaCys: 1.263 ± 0.01	3.187AlaAsp: 3.187 ± 0.014	4.577AlaGlu: 4.577 ± 0.024	2.383AlaPhe: 2.383 ± 0.013	4.282AlaGly: 4.282 ± 0.018	1.5AlaHis: 1.5 ± 0.009	2.648AlaIle: 2.648 ± 0.013	3.358AlaLys: 3.358 ± 0.019	6.398AlaLeu: 6.398 ± 0.027	1.594AlaMet: 1.594 ± 0.012	2.181AlaAsn: 2.181 ± 0.013	3.485AlaPro: 3.485 ± 0.02	2.928AlaGln: 2.928 ± 0.016	3.071AlaArg: 3.071 ± 0.015	5.537AlaSer: 5.537 ± 0.024	3.478AlaThr: 3.478 ± 0.015	4.911AlaVal: 4.911 ± 0.016	0.636AlaTrp: 0.636 ± 0.007	1.491AlaTyr: 1.491 ± 0.01	0.004AlaXaa: 0.004 ± 0.0
Cys	1.154CysAla: 1.154 ± 0.009	0.701CysCys: 0.701 ± 0.008	1.165CysAsp: 1.165 ± 0.013	1.258CysGlu: 1.258 ± 0.014	0.934CysPhe: 0.934 ± 0.009	1.587CysGly: 1.587 ± 0.016	0.668CysHis: 0.668 ± 0.006	0.976CysIle: 0.976 ± 0.009	1.154CysLys: 1.154 ± 0.01	2.195CysLeu: 2.195 ± 0.014	0.481CysMet: 0.481 ± 0.006	0.845CysAsn: 0.845 ± 0.009	1.292CysPro: 1.292 ± 0.014	1.054CysGln: 1.054 ± 0.01	1.302CysArg: 1.302 ± 0.01	2.202CysSer: 2.202 ± 0.018	1.16CysThr: 1.16 ± 0.009	1.541CysVal: 1.541 ± 0.011	0.307CysTrp: 0.307 ± 0.005	0.631CysTyr: 0.631 ± 0.006	0.001CysXaa: 0.001 ± 0.0
Asp	2.947AspAla: 2.947 ± 0.013	1.165AspCys: 1.165 ± 0.011	3.185AspAsp: 3.185 ± 0.023	3.756AspGlu: 3.756 ± 0.022	2.118AspPhe: 2.118 ± 0.012	3.717AspGly: 3.717 ± 0.017	1.208AspHis: 1.208 ± 0.009	2.665AspIle: 2.665 ± 0.015	2.757AspLys: 2.757 ± 0.015	4.914AspLeu: 4.914 ± 0.018	1.297AspMet: 1.297 ± 0.01	1.956AspAsn: 1.956 ± 0.012	2.844AspPro: 2.844 ± 0.014	2.003AspGln: 2.003 ± 0.012	2.848AspArg: 2.848 ± 0.017	4.621AspSer: 4.621 ± 0.018	2.641AspThr: 2.641 ± 0.013	3.31AspVal: 3.31 ± 0.014	0.675AspTrp: 0.675 ± 0.007	1.566AspTyr: 1.566 ± 0.01	0.001AspXaa: 0.001 ± 0.0
Glu	4.722GluAla: 4.722 ± 0.025	1.22GluCys: 1.22 ± 0.013	4.465GluAsp: 4.465 ± 0.021	7.865GluGlu: 7.865 ± 0.05	1.922GluPhe: 1.922 ± 0.011	4.056GluGly: 4.056 ± 0.019	1.456GluHis: 1.456 ± 0.01	2.788GluIle: 2.788 ± 0.015	4.657GluLys: 4.657 ± 0.031	6.1GluLeu: 6.1 ± 0.029	1.732GluMet: 1.732 ± 0.011	2.717GluAsn: 2.717 ± 0.016	2.814GluPro: 2.814 ± 0.017	3.089GluGln: 3.089 ± 0.018	4.237GluArg: 4.237 ± 0.028	4.35GluSer: 4.35 ± 0.02	3.443GluThr: 3.443 ± 0.015	4.301GluVal: 4.301 ± 0.019	0.674GluTrp: 0.674 ± 0.007	1.559GluTyr: 1.559 ± 0.009	0.003GluXaa: 0.003 ± 0.0
Phe	1.919PheAla: 1.919 ± 0.013	1.006PheCys: 1.006 ± 0.009	1.76PheAsp: 1.76 ± 0.009	1.823PheGlu: 1.823 ± 0.012	1.634PhePhe: 1.634 ± 0.012	2.196PheGly: 2.196 ± 0.014	1.036PheHis: 1.036 ± 0.008	1.935PheIle: 1.935 ± 0.013	1.796PheLys: 1.796 ± 0.012	3.904PheLeu: 3.904 ± 0.021	0.84PheMet: 0.84 ± 0.008	1.477PheAsn: 1.477 ± 0.011	1.812PhePro: 1.812 ± 0.01	1.613PheGln: 1.613 ± 0.01	1.863PheArg: 1.863 ± 0.011	3.48PheSer: 3.48 ± 0.015	2.279PheThr: 2.279 ± 0.013	2.282PheVal: 2.282 ± 0.014	0.479PheTrp: 0.479 ± 0.006	1.224PheTyr: 1.224 ± 0.008	0.002PheXaa: 0.002 ± 0.0
Gly	3.933GlyAla: 3.933 ± 0.016	1.243GlyCys: 1.243 ± 0.01	3.22GlyAsp: 3.22 ± 0.014	4.06GlyGlu: 4.06 ± 0.021	2.427GlyPhe: 2.427 ± 0.016	5.469GlyGly: 5.469 ± 0.037	1.665GlyHis: 1.665 ± 0.01	2.571GlyIle: 2.571 ± 0.014	3.582GlyLys: 3.582 ± 0.019	5.393GlyLeu: 5.393 ± 0.019	1.492GlyMet: 1.492 ± 0.012	2.438GlyAsn: 2.438 ± 0.014	3.223GlyPro: 3.223 ± 0.03	2.754GlyGln: 2.754 ± 0.015	3.678GlyArg: 3.678 ± 0.019	5.828GlySer: 5.828 ± 0.025	3.353GlyThr: 3.353 ± 0.016	3.962GlyVal: 3.962 ± 0.017	0.764GlyTrp: 0.764 ± 0.009	1.816GlyTyr: 1.816 ± 0.014	0.004GlyXaa: 0.004 ± 0.0
His	1.316HisAla: 1.316 ± 0.01	0.78HisCys: 0.78 ± 0.008	0.985HisAsp: 0.985 ± 0.008	1.204HisGlu: 1.204 ± 0.01	1.066HisPhe: 1.066 ± 0.008	1.56HisGly: 1.56 ± 0.012	1.142HisHis: 1.142 ± 0.014	1.32HisIle: 1.32 ± 0.009	1.311HisLys: 1.311 ± 0.009	2.742HisLeu: 2.742 ± 0.014	0.709HisMet: 0.709 ± 0.008	1.043HisAsn: 1.043 ± 0.008	1.612HisPro: 1.612 ± 0.011	1.325HisGln: 1.325 ± 0.012	1.703HisArg: 1.703 ± 0.011	2.593HisSer: 2.593 ± 0.015	1.66HisThr: 1.66 ± 0.012	1.442HisVal: 1.442 ± 0.01	0.33HisTrp: 0.33 ± 0.005	0.856HisTyr: 0.856 ± 0.006	0.001HisXaa: 0.001 ± 0.0
Ile	2.459IleAla: 2.459 ± 0.013	1.09IleCys: 1.09 ± 0.009	2.075IleAsp: 2.075 ± 0.014	2.338IleGlu: 2.338 ± 0.014	1.801IlePhe: 1.801 ± 0.013	2.266IleGly: 2.266 ± 0.013	1.294IleHis: 1.294 ± 0.011	2.37IleIle: 2.37 ± 0.014	2.487IleLys: 2.487 ± 0.012	4.25IleLeu: 4.25 ± 0.02	1.06IleMet: 1.06 ± 0.008	1.926IleAsn: 1.926 ± 0.012	2.459IlePro: 2.459 ± 0.013	2.186IleGln: 2.186 ± 0.013	2.426IleArg: 2.426 ± 0.012	3.805IleSer: 3.805 ± 0.018	2.741IleThr: 2.741 ± 0.015	2.532IleVal: 2.532 ± 0.014	0.474IleTrp: 0.474 ± 0.005	1.387IleTyr: 1.387 ± 0.009	0.002IleXaa: 0.002 ± 0.0
Lys	3.736LysAla: 3.736 ± 0.018	1.036LysCys: 1.036 ± 0.011	3.241LysAsp: 3.241 ± 0.017	4.678LysGlu: 4.678 ± 0.025	1.603LysPhe: 1.603 ± 0.011	3.12LysGly: 3.12 ± 0.017	1.421LysHis: 1.421 ± 0.01	2.445LysIle: 2.445 ± 0.013	4.447LysLys: 4.447 ± 0.029	4.884LysLeu: 4.884 ± 0.021	1.488LysMet: 1.488 ± 0.01	2.255LysAsn: 2.255 ± 0.012	2.997LysPro: 2.997 ± 0.018	2.556LysGln: 2.556 ± 0.015	3.43LysArg: 3.43 ± 0.016	3.861LysSer: 3.861 ± 0.018	3.274LysThr: 3.274 ± 0.017	3.521LysVal: 3.521 ± 0.016	0.558LysTrp: 0.558 ± 0.005	1.474LysTyr: 1.474 ± 0.011	0.002LysXaa: 0.002 ± 0.0
Leu	5.953LeuAla: 5.953 ± 0.025	2.247LeuCys: 2.247 ± 0.016	4.862LeuAsp: 4.862 ± 0.018	6.309LeuGlu: 6.309 ± 0.029	3.42LeuPhe: 3.42 ± 0.018	5.09LeuGly: 5.09 ± 0.021	2.747LeuHis: 2.747 ± 0.016	3.802LeuIle: 3.802 ± 0.018	5.536LeuLys: 5.536 ± 0.022	10.043LeuLeu: 10.043 ± 0.043	2.147LeuMet: 2.147 ± 0.012	3.599LeuAsn: 3.599 ± 0.016	5.347LeuPro: 5.347 ± 0.023	5.493LeuGln: 5.493 ± 0.024	5.57LeuArg: 5.57 ± 0.022	8.392LeuSer: 8.392 ± 0.027	5.25LeuThr: 5.25 ± 0.016	5.413LeuVal: 5.413 ± 0.021	1.077LeuTrp: 1.077 ± 0.008	2.607LeuTyr: 2.607 ± 0.013	0.005LeuXaa: 0.005 ± 0.0
Met	1.915MetAla: 1.915 ± 0.011	0.491MetCys: 0.491 ± 0.006	1.46MetAsp: 1.46 ± 0.009	1.994MetGlu: 1.994 ± 0.011	0.873MetPhe: 0.873 ± 0.007	1.428MetGly: 1.428 ± 0.01	0.501MetHis: 0.501 ± 0.005	0.881MetIle: 0.881 ± 0.008	1.506MetLys: 1.506 ± 0.01	2.109MetLeu: 2.109 ± 0.011	0.721MetMet: 0.721 ± 0.007	0.93MetAsn: 0.93 ± 0.008	1.117MetPro: 1.117 ± 0.01	1.017MetGln: 1.017 ± 0.008	1.24MetArg: 1.24 ± 0.01	1.93MetSer: 1.93 ± 0.011	1.293MetThr: 1.293 ± 0.008	1.528MetVal: 1.528 ± 0.009	0.265MetTrp: 0.265 ± 0.004	0.63MetTyr: 0.63 ± 0.007	0.001MetXaa: 0.001 ± 0.0
Asn	2.162AsnAla: 2.162 ± 0.012	0.872AsnCys: 0.872 ± 0.008	1.669AsnAsp: 1.669 ± 0.013	2.07AsnGlu: 2.07 ± 0.012	1.417AsnPhe: 1.417 ± 0.01	2.781AsnGly: 2.781 ± 0.018	1.032AsnHis: 1.032 ± 0.008	2.107AsnIle: 2.107 ± 0.012	2.187AsnLys: 2.187 ± 0.012	3.569AsnLeu: 3.569 ± 0.017	1.054AsnMet: 1.054 ± 0.008	1.797AsnAsn: 1.797 ± 0.014	2.24AsnPro: 2.24 ± 0.014	1.789AsnGln: 1.789 ± 0.012	2.01AsnArg: 2.01 ± 0.012	3.264AsnSer: 3.264 ± 0.015	2.268AsnThr: 2.268 ± 0.012	2.287AsnVal: 2.287 ± 0.012	0.429AsnTrp: 0.429 ± 0.006	1.122AsnTyr: 1.122 ± 0.009	0.002AsnXaa: 0.002 ± 0.0
Pro	4.226ProAla: 4.226 ± 0.021	1.069ProCys: 1.069 ± 0.012	2.927ProAsp: 2.927 ± 0.014	3.775ProGlu: 3.775 ± 0.016	1.814ProPhe: 1.814 ± 0.013	3.962ProGly: 3.962 ± 0.032	1.535ProHis: 1.535 ± 0.012	1.884ProIle: 1.884 ± 0.01	2.559ProLys: 2.559 ± 0.018	4.892ProLeu: 4.892 ± 0.019	1.033ProMet: 1.033 ± 0.009	1.935ProAsn: 1.935 ± 0.012	5.619ProPro: 5.619 ± 0.044	2.757ProGln: 2.757 ± 0.025	2.751ProArg: 2.751 ± 0.018	5.722ProSer: 5.722 ± 0.032	3.144ProThr: 3.144 ± 0.019	3.797ProVal: 3.797 ± 0.017	0.549ProTrp: 0.549 ± 0.005	1.47ProTyr: 1.47 ± 0.011	0.008ProXaa: 0.008 ± 0.001
Gln	3.153GlnAla: 3.153 ± 0.018	0.971GlnCys: 0.971 ± 0.01	2.351GlnAsp: 2.351 ± 0.012	3.513GlnGlu: 3.513 ± 0.022	1.354GlnPhe: 1.354 ± 0.009	2.708GlnGly: 2.708 ± 0.017	1.397GlnHis: 1.397 ± 0.012	1.922GlnIle: 1.922 ± 0.011	2.603GlnLys: 2.603 ± 0.016	4.535GlnLeu: 4.535 ± 0.023	1.161GlnMet: 1.161 ± 0.01	1.807GlnAsn: 1.807 ± 0.01	2.691GlnPro: 2.691 ± 0.02	3.522GlnGln: 3.522 ± 0.035	3.145GlnArg: 3.145 ± 0.015	3.609GlnSer: 3.609 ± 0.019	2.675GlnThr: 2.675 ± 0.015	2.816GlnVal: 2.816 ± 0.016	0.542GlnTrp: 0.542 ± 0.006	1.23GlnTyr: 1.23 ± 0.008	0.002GlnXaa: 0.002 ± 0.0
Arg	3.379ArgAla: 3.379 ± 0.016	1.295ArgCys: 1.295 ± 0.013	2.95ArgAsp: 2.95 ± 0.016	3.889ArgGlu: 3.889 ± 0.022	1.968ArgPhe: 1.968 ± 0.011	3.428ArgGly: 3.428 ± 0.019	1.631ArgHis: 1.631 ± 0.011	2.372ArgIle: 2.372 ± 0.013	3.564ArgLys: 3.564 ± 0.017	5.331ArgLeu: 5.331 ± 0.021	1.32ArgMet: 1.32 ± 0.009	2.124ArgAsn: 2.124 ± 0.01	3.006ArgPro: 3.006 ± 0.019	2.717ArgGln: 2.717 ± 0.014	4.485ArgArg: 4.485 ± 0.024	4.664ArgSer: 4.664 ± 0.026	2.994ArgThr: 2.994 ± 0.017	3.254ArgVal: 3.254 ± 0.015	0.686ArgTrp: 0.686 ± 0.006	1.59ArgTyr: 1.59 ± 0.01	0.002ArgXaa: 0.002 ± 0.0
Ser	5.664SerAla: 5.664 ± 0.021	2.046SerCys: 2.046 ± 0.015	4.469SerAsp: 4.469 ± 0.02	4.963SerGlu: 4.963 ± 0.023	3.173SerPhe: 3.173 ± 0.016	5.699SerGly: 5.699 ± 0.021	2.317SerHis: 2.317 ± 0.013	3.368SerIle: 3.368 ± 0.017	4.059SerLys: 4.059 ± 0.02	8.401SerLeu: 8.401 ± 0.027	1.866SerMet: 1.866 ± 0.01	3.04SerAsn: 3.04 ± 0.014	6.139SerPro: 6.139 ± 0.035	3.974SerGln: 3.974 ± 0.018	4.659SerArg: 4.659 ± 0.022	10.964SerSer: 10.964 ± 0.052	4.97SerThr: 4.97 ± 0.022	5.561SerVal: 5.561 ± 0.019	0.995SerTrp: 0.995 ± 0.008	2.2SerTyr: 2.2 ± 0.014	0.004SerXaa: 0.004 ± 0.0
Thr	4.07ThrAla: 4.07 ± 0.015	1.375ThrCys: 1.375 ± 0.015	2.981ThrAsp: 2.981 ± 0.015	3.753ThrGlu: 3.753 ± 0.017	2.111ThrPhe: 2.111 ± 0.011	3.705ThrGly: 3.705 ± 0.017	1.463ThrHis: 1.463 ± 0.01	2.397ThrIle: 2.397 ± 0.014	2.68ThrLys: 2.68 ± 0.014	5.318ThrLeu: 5.318 ± 0.019	1.231ThrMet: 1.231 ± 0.009	1.963ThrAsn: 1.963 ± 0.011	3.694ThrPro: 3.694 ± 0.022	2.373ThrGln: 2.373 ± 0.014	2.501ThrArg: 2.501 ± 0.012	4.985ThrSer: 4.985 ± 0.018	3.461ThrThr: 3.461 ± 0.026	4.216ThrVal: 4.216 ± 0.017	0.653ThrTrp: 0.653 ± 0.006	1.432ThrTyr: 1.432 ± 0.011	0.003ThrXaa: 0.003 ± 0.0
Val	4.132ValAla: 4.132 ± 0.016	1.766ValCys: 1.766 ± 0.015	3.207ValAsp: 3.207 ± 0.014	4.035ValGlu: 4.035 ± 0.019	2.667ValPhe: 2.667 ± 0.015	3.516ValGly: 3.516 ± 0.014	1.608ValHis: 1.608 ± 0.01	2.977ValIle: 2.977 ± 0.016	3.615ValLys: 3.615 ± 0.018	6.173ValLeu: 6.173 ± 0.024	1.566ValMet: 1.566 ± 0.01	2.417ValAsn: 2.417 ± 0.014	3.27ValPro: 3.27 ± 0.018	2.808ValGln: 2.808 ± 0.016	3.28ValArg: 3.28 ± 0.015	5.383ValSer: 5.383 ± 0.02	3.94ValThr: 3.94 ± 0.018	4.429ValVal: 4.429 ± 0.021	0.781ValTrp: 0.781 ± 0.008	1.788ValTyr: 1.788 ± 0.011	0.002ValXaa: 0.002 ± 0.0
Trp	0.653TrpAla: 0.653 ± 0.007	0.246TrpCys: 0.246 ± 0.004	0.645TrpAsp: 0.645 ± 0.006	0.737TrpGlu: 0.737 ± 0.007	0.471TrpPhe: 0.471 ± 0.005	0.622TrpGly: 0.622 ± 0.009	0.274TrpHis: 0.274 ± 0.004	0.543TrpIle: 0.543 ± 0.007	0.716TrpLys: 0.716 ± 0.007	1.128TrpLeu: 1.128 ± 0.009	0.354TrpMet: 0.354 ± 0.004	0.5TrpAsn: 0.5 ± 0.006	0.432TrpPro: 0.432 ± 0.005	0.49TrpGln: 0.49 ± 0.005	0.753TrpArg: 0.753 ± 0.007	0.947TrpSer: 0.947 ± 0.009	0.717TrpThr: 0.717 ± 0.008	0.685TrpVal: 0.685 ± 0.006	0.19TrpTrp: 0.19 ± 0.003	0.341TrpTyr: 0.341 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.418TyrAla: 1.418 ± 0.01	0.726TyrCys: 0.726 ± 0.008	1.391TyrAsp: 1.391 ± 0.01	1.57TyrGlu: 1.57 ± 0.01	1.204TyrPhe: 1.204 ± 0.009	1.679TyrGly: 1.679 ± 0.012	0.801TyrHis: 0.801 ± 0.008	1.456TyrIle: 1.456 ± 0.009	1.45TyrLys: 1.45 ± 0.013	2.626TyrLeu: 2.626 ± 0.013	0.679TyrMet: 0.679 ± 0.007	1.176TyrAsn: 1.176 ± 0.008	1.325TyrPro: 1.325 ± 0.01	1.258TyrGln: 1.258 ± 0.009	1.696TyrArg: 1.696 ± 0.011	2.39TyrSer: 2.39 ± 0.014	1.619TyrThr: 1.619 ± 0.011	1.566TyrVal: 1.566 ± 0.011	0.38TyrTrp: 0.38 ± 0.006	0.974TyrTyr: 0.974 ± 0.008	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.005XaaGly: 0.005 ± 0.0	0.002XaaHis: 0.002 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.004XaaLeu: 0.004 ± 0.001	0.002XaaMet: 0.002 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.007XaaPro: 0.007 ± 0.001	0.002XaaGln: 0.002 ± 0.0	0.003XaaArg: 0.003 ± 0.0	0.005XaaSer: 0.005 ± 0.001	0.002XaaThr: 0.002 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.572XaaXaa: 0.572 ± 0.064

Statistics based on 33480 proteins (18433522 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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