cyanobiont of Ornithocercus magnificus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; unclassified Synechococcales

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y3GAV8|A0A4Y3GAV8_9CYAN Uncharacterized protein OS=cyanobiont of Ornithocercus magnificus OX=2496102 GN=OMCYN_01631 PE=4 SV=1
MM1 pKa = 6.97VRR3 pKa = 11.84AIQARR8 pKa = 11.84AVDD11 pKa = 4.12SQADD15 pKa = 3.98SIPTGSYY22 pKa = 9.73DD23 pKa = 4.98QKK25 pKa = 11.13QPWDD29 pKa = 3.39AVEE32 pKa = 4.77TYY34 pKa = 9.74FEE36 pKa = 5.34CITTCSLDD44 pKa = 4.36DD45 pKa = 4.21GDD47 pKa = 5.61CITRR51 pKa = 11.84CVEE54 pKa = 3.84QLRR57 pKa = 11.84EE58 pKa = 3.6ADD60 pKa = 3.77YY61 pKa = 11.66

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y3FV91|A0A4Y3FV91_9CYAN Uncharacterized protein OS=cyanobiont of Ornithocercus magnificus OX=2496102 GN=OMCYN_01887 PE=4 SV=1
MM1 pKa = 7.46TKK3 pKa = 8.36HH4 pKa = 5.56TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 5.95TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84KK34 pKa = 7.52RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84TQLAVV45 pKa = 3.09

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1841

0

1841

510641

30

1544

277.4

30.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.214 ± 0.06

1.556 ± 0.024

4.853 ± 0.04

5.566 ± 0.048

3.199 ± 0.04

7.527 ± 0.043

2.317 ± 0.03

5.437 ± 0.045

3.193 ± 0.042

12.605 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.025

3.169 ± 0.034

5.041 ± 0.042

4.451 ± 0.04

7.13 ± 0.048

7.005 ± 0.037

5.024 ± 0.039

6.767 ± 0.045

1.692 ± 0.03

2.258 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski