Klebsiella quasipneumoniae subsp. similipneumoniae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A663B3H6|A0A663B3H6_9ENTR Uncharacterized protein OS=Klebsiella quasipneumoniae subsp. similipneumoniae OX=1463164 GN=SB00610_01721 PE=4 SV=1
MM1 pKa = 7.63SDD3 pKa = 3.42AFFEE7 pKa = 4.28EE8 pKa = 4.82LTDD11 pKa = 3.83GDD13 pKa = 5.25AIPDD17 pKa = 3.48QSQFDD22 pKa = 3.06IWYY25 pKa = 9.67EE26 pKa = 3.95EE27 pKa = 4.11LCAYY31 pKa = 10.4ALDD34 pKa = 3.95HH35 pKa = 6.96SGRR38 pKa = 11.84APYY41 pKa = 10.25KK42 pKa = 9.02MAWLEE47 pKa = 3.91FFEE50 pKa = 4.57QGMTVEE56 pKa = 4.26VAFDD60 pKa = 3.35KK61 pKa = 10.93FYY63 pKa = 11.12EE64 pKa = 4.16

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A663BJW4|A0A663BJW4_9ENTR Putative hydrolase YxeP OS=Klebsiella quasipneumoniae subsp. similipneumoniae OX=1463164 GN=yxeP_3 PE=4 SV=1
MM1 pKa = 7.41HH2 pKa = 7.72LILLRR7 pKa = 11.84IRR9 pKa = 11.84RR10 pKa = 11.84DD11 pKa = 3.28NFITLIQRR19 pKa = 11.84IIIIGRR25 pKa = 11.84QRR27 pKa = 11.84QINILRR33 pKa = 11.84AVWQGIGQIARR44 pKa = 11.84VLIANRR50 pKa = 11.84RR51 pKa = 11.84QQ52 pKa = 3.04

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5434

0

5434

1237787

29

1660

227.8

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.204 ± 0.044

1.099 ± 0.014

5.164 ± 0.029

5.405 ± 0.037

4.033 ± 0.025

7.52 ± 0.037

2.538 ± 0.024

5.654 ± 0.028

3.984 ± 0.034

10.772 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.015

3.462 ± 0.025

4.476 ± 0.025

4.642 ± 0.027

6.289 ± 0.031

5.65 ± 0.031

5.089 ± 0.025

7.14 ± 0.028

1.475 ± 0.017

2.653 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski