Drosophila kikkawai (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P4I829|A0A6P4I829_DROKI uncharacterized protein LOC108076654 OS=Drosophila kikkawai OX=30033 GN=LOC108076654 PE=4 SV=1
MM1 pKa = 7.2QSKK4 pKa = 10.56LCVVLLALGLSQILAVPAPSLVLTHH29 pKa = 7.18PEE31 pKa = 3.95TNFMQFLMQSRR42 pKa = 11.84DD43 pKa = 3.3LANDD47 pKa = 3.61GTHH50 pKa = 6.71SLEE53 pKa = 4.15CLSYY57 pKa = 8.74YY58 pKa = 10.41TPLLNSAVEE67 pKa = 4.32KK68 pKa = 10.83YY69 pKa = 11.13NNDD72 pKa = 2.84FKK74 pKa = 11.66ACLDD78 pKa = 3.69EE79 pKa = 4.76ATLQVAAINDD89 pKa = 3.66DD90 pKa = 3.64TKK92 pKa = 11.47EE93 pKa = 3.83NRR95 pKa = 11.84TLIDD99 pKa = 3.14QSATDD104 pKa = 3.35SCAALEE110 pKa = 4.41TCSQLTAADD119 pKa = 4.72EE120 pKa = 4.29YY121 pKa = 11.03FEE123 pKa = 5.19CYY125 pKa = 10.21SEE127 pKa = 4.96AGSSNAKK134 pKa = 10.53SMFTISANASEE145 pKa = 4.15LLAYY149 pKa = 10.38VKK151 pKa = 10.2EE152 pKa = 4.15QVHH155 pKa = 6.61IIQVNEE161 pKa = 4.09YY162 pKa = 8.98ICTNKK167 pKa = 8.13TQRR170 pKa = 11.84TYY172 pKa = 11.64AEE174 pKa = 4.21DD175 pKa = 3.3TADD178 pKa = 3.26IYY180 pKa = 11.41EE181 pKa = 4.25SLSLCFSGAPIPTEE195 pKa = 4.11TSSTAAPEE203 pKa = 4.37TSSSLDD209 pKa = 3.59PEE211 pKa = 4.51SSTDD215 pKa = 3.36SSSSEE220 pKa = 4.15PEE222 pKa = 3.93SSSTAEE228 pKa = 4.38PEE230 pKa = 4.2SSSTDD235 pKa = 3.14SSSAEE240 pKa = 4.06PEE242 pKa = 4.39SSSSAAPEE250 pKa = 4.03SSTDD254 pKa = 3.33SSSVEE259 pKa = 4.06PEE261 pKa = 4.13SSSTAAPEE269 pKa = 4.02SSTDD273 pKa = 3.26SSSAEE278 pKa = 4.06PEE280 pKa = 4.39SSSSAAPEE288 pKa = 4.47DD289 pKa = 3.94SSSAEE294 pKa = 4.13PEE296 pKa = 4.39SSSSAAPEE304 pKa = 4.47DD305 pKa = 3.94SSSAEE310 pKa = 4.17PEE312 pKa = 4.32SSSTAAPEE320 pKa = 4.52DD321 pKa = 3.85SSSAEE326 pKa = 4.13PEE328 pKa = 4.39SSSSAAPEE336 pKa = 4.47DD337 pKa = 3.94SSSAEE342 pKa = 4.18PEE344 pKa = 4.17SSSAAPEE351 pKa = 4.08SSTAEE356 pKa = 4.2PEE358 pKa = 4.45SSSSAAPDD366 pKa = 3.52APEE369 pKa = 4.45EE370 pKa = 4.04DD371 pKa = 3.68LKK373 pKa = 11.28QIYY376 pKa = 8.72TGNSNKK382 pKa = 9.77NLQDD386 pKa = 3.18ILKK389 pKa = 10.2NLQTWFKK396 pKa = 7.99TQRR399 pKa = 11.84AA400 pKa = 3.9

Molecular weight: 41.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P4IPG3|A0A6P4IPG3_DROKI Cell division protein kinase 7 OS=Drosophila kikkawai OX=30033 GN=LOC108080235 PE=3 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84LLLAIVGCLFCALFGQTSGTPEE24 pKa = 4.13CRR26 pKa = 11.84PPLVILPKK34 pKa = 10.01PDD36 pKa = 3.07INSRR40 pKa = 11.84RR41 pKa = 11.84ARR43 pKa = 11.84PVIRR47 pKa = 11.84PLPRR51 pKa = 11.84PPPPKK56 pKa = 9.99VVRR59 pKa = 11.84PPVKK63 pKa = 10.12PPIKK67 pKa = 9.88RR68 pKa = 11.84PPIVPPRR75 pKa = 11.84ITRR78 pKa = 11.84RR79 pKa = 11.84PITPPRR85 pKa = 11.84ITRR88 pKa = 11.84RR89 pKa = 11.84PPIPPPRR96 pKa = 11.84ITRR99 pKa = 11.84RR100 pKa = 11.84PPIQPPRR107 pKa = 11.84ITRR110 pKa = 11.84RR111 pKa = 11.84PPIPPVTRR119 pKa = 11.84KK120 pKa = 7.21PTRR123 pKa = 11.84PPVTQRR129 pKa = 11.84ATAKK133 pKa = 8.87ATTRR137 pKa = 11.84KK138 pKa = 9.36FLIWSKK144 pKa = 10.78SPTRR148 pKa = 11.84RR149 pKa = 11.84VPTRR153 pKa = 11.84PNIRR157 pKa = 11.84PTLWTRR163 pKa = 11.84PNIRR167 pKa = 11.84PTRR170 pKa = 11.84PPVRR174 pKa = 11.84PPSPTVRR181 pKa = 11.84PTVPRR186 pKa = 11.84VRR188 pKa = 11.84PTLWTRR194 pKa = 11.84PPVRR198 pKa = 11.84VTVRR202 pKa = 11.84PTNRR206 pKa = 11.84APVTRR211 pKa = 11.84PPVRR215 pKa = 11.84PTNRR219 pKa = 11.84ATLWTRR225 pKa = 11.84NPMGTTRR232 pKa = 11.84PPRR235 pKa = 11.84PTMKK239 pKa = 10.26PSRR242 pKa = 11.84PTLWTRR248 pKa = 11.84SPTRR252 pKa = 11.84STQQTTRR259 pKa = 11.84HH260 pKa = 4.92LTFIFEE266 pKa = 4.45GSSAAPVTPMDD277 pKa = 3.67VTEE280 pKa = 5.1DD281 pKa = 3.68PQITRR286 pKa = 11.84TTKK289 pKa = 9.96SLIFIFPRR297 pKa = 11.84DD298 pKa = 3.65TTAMARR304 pKa = 11.84GPNWRR309 pKa = 11.84VHH311 pKa = 4.35PTGRR315 pKa = 11.84PRR317 pKa = 11.84RR318 pKa = 11.84VTMVSS323 pKa = 2.91

Molecular weight: 36.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15043

4949

19992

12791145

32

18810

639.8

71.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.849 ± 0.02

1.72 ± 0.024

5.156 ± 0.014

6.542 ± 0.025

3.308 ± 0.014

6.354 ± 0.023

2.655 ± 0.01

4.665 ± 0.014

5.415 ± 0.018

9.048 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.009

4.589 ± 0.012

5.626 ± 0.024

5.54 ± 0.027

5.562 ± 0.015

8.44 ± 0.027

5.688 ± 0.014

5.796 ± 0.015

0.956 ± 0.005

2.806 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski