Eimeria praecox
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7635 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U6H057|U6H057_9EIME Uncharacterized protein OS=Eimeria praecox OX=51316 GN=EPH_0017690 PE=4 SV=1
MM1 pKa = 7.17 LWAYY5 pKa = 10.84 VYY7 pKa = 11.23 LNLYY11 pKa = 9.66 KK12 pKa = 10.18 IYY14 pKa = 10.63 AAAAADD20 pKa = 4.88 DD21 pKa = 5.36 DD22 pKa = 5.95 DD23 pKa = 7.66 DD24 pKa = 6.5 DD25 pKa = 6.48 VDD27 pKa = 5.84 DD28 pKa = 6.19 DD29 pKa = 4.99 DD30 pKa = 4.38 VAAAAALGAVAVKK43 pKa = 10.16 QRR45 pKa = 11.84 AQAAAAAAAAVLQQLLLQQQQQQQQMVYY73 pKa = 10.07 VVLLSGRR80 pKa = 11.84 LSSSEE85 pKa = 4.04 CMQTAADD92 pKa = 3.94 TAAATAAATAAAIAAAAAIAAAAAAAAAAAYY123 pKa = 10.1 RR124 pKa = 11.84 LL125 pKa = 3.86
Molecular weight: 12.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.821
IPC_protein 3.783
Toseland 3.541
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.821
Rodwell 3.605
Grimsley 3.465
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.266
Thurlkill 3.643
EMBOSS 3.821
Sillero 3.91
Patrickios 0.782
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|U6GTK9|U6GTK9_9EIME Uncharacterized protein OS=Eimeria praecox OX=51316 GN=EPH_0010890 PE=4 SV=1
MM1 pKa = 7.53 GPPRR5 pKa = 11.84 GPLNRR10 pKa = 11.84 APKK13 pKa = 9.53 RR14 pKa = 11.84 APKK17 pKa = 9.76 RR18 pKa = 11.84 APKK21 pKa = 10.07 GPPKK25 pKa = 10.3 GPPKK29 pKa = 10.47 GPPKK33 pKa = 10.21 RR34 pKa = 11.84 AFQGPPKK41 pKa = 9.72 RR42 pKa = 11.84 APKK45 pKa = 10.2 GPPKK49 pKa = 10.33 RR50 pKa = 11.84 PPNGTLKK57 pKa = 10.42 RR58 pKa = 11.84 APKK61 pKa = 9.64 GAPKK65 pKa = 10.48 GPPNTPHH72 pKa = 7.13 GGGPPKK78 pKa = 10.4 GPPKK82 pKa = 10.51 GPPKK86 pKa = 10.67 GPLKK90 pKa = 10.18 RR91 pKa = 11.84 APKK94 pKa = 9.86 GAPP97 pKa = 3.06
Molecular weight: 9.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.499
IPC2_protein 11.228
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.939
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.896
Grimsley 12.969
Solomon 13.408
Lehninger 13.32
Nozaki 12.925
DTASelect 12.91
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.618
IPC_peptide 13.422
IPC2_peptide 12.398
IPC2.peptide.svr19 9.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7635
0
7635
3653206
42
7677
478.5
51.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.597 ± 0.123
1.614 ± 0.017
3.433 ± 0.025
6.053 ± 0.043
1.993 ± 0.02
5.193 ± 0.038
2.164 ± 0.016
2.409 ± 0.02
3.301 ± 0.029
8.605 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.332 ± 0.012
2.496 ± 0.026
5.026 ± 0.038
14.76 ± 0.156
5.332 ± 0.032
9.562 ± 0.059
4.865 ± 0.027
4.161 ± 0.032
0.773 ± 0.008
1.331 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here