Mycobacterium phage CaptainTrips
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0RLU9|A0A0A0RLU9_9CAUD Glycosyltransferase OS=Mycobacterium phage CaptainTrips OX=1556289 GN=103 PE=4 SV=1
MM1 pKa = 7.98 PDD3 pKa = 3.43 SDD5 pKa = 5.65 PIDD8 pKa = 3.45 FTAAGEE14 pKa = 4.07 AFAEE18 pKa = 4.23 IFMDD22 pKa = 4.96 GIRR25 pKa = 11.84 AIIAQEE31 pKa = 3.5 LDD33 pKa = 3.05 ARR35 pKa = 11.84 GVKK38 pKa = 10.45 GPSTVVNNVVPYY50 pKa = 10.34 SLPDD54 pKa = 3.52 SQDD57 pKa = 2.82 AQYY60 pKa = 11.04 IEE62 pKa = 4.16 ATYY65 pKa = 10.25 EE66 pKa = 3.86 ADD68 pKa = 3.32 VV69 pKa = 3.7
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.149
IPC2_protein 3.63
IPC_protein 3.554
Toseland 3.35
ProMoST 3.757
Dawson 3.567
Bjellqvist 3.732
Wikipedia 3.541
Rodwell 3.389
Grimsley 3.274
Solomon 3.516
Lehninger 3.478
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.541
Sillero 3.681
Patrickios 3.083
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.695
Protein with the highest isoelectric point:
>tr|A0A0A0RLP9|A0A0A0RLP9_9CAUD Tail assembly chaperone OS=Mycobacterium phage CaptainTrips OX=1556289 GN=13 PE=4 SV=1
MM1 pKa = 6.68 TQRR4 pKa = 11.84 KK5 pKa = 9.5 GGFDD9 pKa = 4.35 WIRR12 pKa = 11.84 STYY15 pKa = 9.77 RR16 pKa = 11.84 VPAKK20 pKa = 10.02 RR21 pKa = 11.84 GMRR24 pKa = 11.84 VVFDD28 pKa = 3.72 GRR30 pKa = 11.84 PGRR33 pKa = 11.84 ILSVDD38 pKa = 3.4 GPYY41 pKa = 11.37 LMLHH45 pKa = 6.91 LDD47 pKa = 3.96 SDD49 pKa = 4.41 PKK51 pKa = 10.37 NLRR54 pKa = 11.84 TRR56 pKa = 11.84 VHH58 pKa = 5.46 PTWRR62 pKa = 11.84 MEE64 pKa = 3.91 YY65 pKa = 10.61 LPP67 pKa = 4.85
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.94
IPC_protein 11.008
Toseland 10.965
ProMoST 11.052
Dawson 11.038
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.038
Grimsley 11.096
Solomon 11.301
Lehninger 11.242
Nozaki 10.935
DTASelect 10.877
Thurlkill 10.965
EMBOSS 11.403
Sillero 10.994
Patrickios 10.804
IPC_peptide 11.301
IPC2_peptide 10.072
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
107
0
107
18442
28
1174
172.4
18.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.829 ± 0.467
1.28 ± 0.164
6.61 ± 0.213
5.872 ± 0.305
2.95 ± 0.189
8.481 ± 0.527
2.261 ± 0.192
4.295 ± 0.174
3.34 ± 0.178
7.402 ± 0.185
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.144
3.286 ± 0.16
6.106 ± 0.22
3.438 ± 0.202
6.935 ± 0.401
5.807 ± 0.274
6.648 ± 0.213
7.315 ± 0.269
2.332 ± 0.148
2.462 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here