Pelomicrobium methylotrophicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Hydrogenophilalia; Hydrogenophilales; Hydrogenophilales incertae sedis; Pelomicrobium

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C7ESQ5|A0A5C7ESQ5_9PROT DNA internalization-related competence protein ComEC/Rec2 OS=Pelomicrobium methylotrophicum OX=2602750 GN=FR698_09030 PE=4 SV=1
MM1 pKa = 7.71AYY3 pKa = 9.35IATVKK8 pKa = 10.41IVVDD12 pKa = 3.86EE13 pKa = 4.68ANEE16 pKa = 3.72AMVYY20 pKa = 10.69DD21 pKa = 5.28GINEE25 pKa = 4.11ILRR28 pKa = 11.84EE29 pKa = 4.06AQYY32 pKa = 11.14GGPNGEE38 pKa = 3.89FRR40 pKa = 11.84GWIADD45 pKa = 3.43WAFWSVEE52 pKa = 3.9PASEE56 pKa = 4.06EE57 pKa = 4.27VEE59 pKa = 4.04KK60 pKa = 11.27SLASGNYY67 pKa = 9.02EE68 pKa = 3.85EE69 pKa = 6.16GEE71 pKa = 4.17AFPDD75 pKa = 3.88CPARR79 pKa = 11.84STAEE83 pKa = 3.98PSSGCSS89 pKa = 3.04

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C7ELF2|A0A5C7ELF2_9PROT D-alanine--D-alanine ligase OS=Pelomicrobium methylotrophicum OX=2602750 GN=ddl PE=3 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84DD3 pKa = 3.05TVRR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 8.03MAAGGYY14 pKa = 6.75VHH16 pKa = 6.52YY17 pKa = 10.37RR18 pKa = 11.84ALLCLGKK25 pKa = 10.37LDD27 pKa = 4.06GLEE30 pKa = 4.23TATSCARR37 pKa = 11.84GLLRR41 pKa = 11.84AHH43 pKa = 6.38SVAVSLRR50 pKa = 11.84TVEE53 pKa = 4.09RR54 pKa = 11.84ACRR57 pKa = 11.84SLRR60 pKa = 11.84AEE62 pKa = 3.83LGMFRR67 pKa = 11.84LAKK70 pKa = 7.62GQKK73 pKa = 8.15WAFPLEE79 pKa = 4.56TKK81 pKa = 9.34WLFCAKK87 pKa = 7.38TTLFRR92 pKa = 5.88

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3202

0

3202

968774

23

2552

302.6

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.03 ± 0.061

1.014 ± 0.017

4.841 ± 0.027

6.463 ± 0.045

3.552 ± 0.03

8.129 ± 0.04

2.223 ± 0.022

4.554 ± 0.031

3.431 ± 0.036

10.939 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.02

2.338 ± 0.023

5.422 ± 0.032

3.368 ± 0.026

8.195 ± 0.048

4.68 ± 0.025

4.711 ± 0.029

7.961 ± 0.04

1.417 ± 0.018

2.506 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski