Sphingobium sp. CAP-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5YKK7|A0A6I5YKK7_9SPHN DNA polymerase I OS=Sphingobium sp. CAP-1 OX=2676077 GN=polA PE=3 SV=1
MM1 pKa = 7.79PKK3 pKa = 10.4LIVVNRR9 pKa = 11.84AGEE12 pKa = 4.04EE13 pKa = 3.9QAVDD17 pKa = 3.52GDD19 pKa = 3.97NGLSVMEE26 pKa = 4.52VIRR29 pKa = 11.84DD30 pKa = 3.58NGFDD34 pKa = 3.96EE35 pKa = 5.76LLALCGGCCSCATCHH50 pKa = 6.48VYY52 pKa = 10.53VDD54 pKa = 3.67PAFADD59 pKa = 3.61VLPTLSEE66 pKa = 4.44DD67 pKa = 3.92EE68 pKa = 4.53NDD70 pKa = 4.31LLDD73 pKa = 4.81SSDD76 pKa = 3.59HH77 pKa = 6.39RR78 pKa = 11.84NEE80 pKa = 3.85TSRR83 pKa = 11.84LSCQLVLSDD92 pKa = 4.83ALDD95 pKa = 3.8GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5YRP2|A0A6I5YRP2_9SPHN LLM class flavin-dependent oxidoreductase OS=Sphingobium sp. CAP-1 OX=2676077 GN=GL174_19480 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4137

0

4137

1348542

28

4224

326.0

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.385 ± 0.054

0.792 ± 0.013

6.207 ± 0.032

4.954 ± 0.037

3.492 ± 0.024

8.937 ± 0.052

2.051 ± 0.021

5.208 ± 0.024

2.981 ± 0.027

9.907 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.021

2.559 ± 0.028

5.34 ± 0.033

3.282 ± 0.021

7.319 ± 0.039

5.212 ± 0.036

5.149 ± 0.031

6.906 ± 0.022

1.453 ± 0.017

2.332 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski