Thermoplasmatales archaeon Gpl
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1917 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0LVC9|T0LVC9_9ARCH Uncharacterized protein OS=Thermoplasmatales archaeon Gpl OX=261391 GN=AMDU5_GPLC00016G0017 PE=4 SV=1
MM1 pKa = 6.99 EE2 pKa = 4.7 TEE4 pKa = 4.62 CNVCGGSLQVPDD16 pKa = 5.1 DD17 pKa = 4.37 VIKK20 pKa = 11.21 GEE22 pKa = 4.27 IVSCPDD28 pKa = 3.08 CGVEE32 pKa = 4.13 YY33 pKa = 10.06 EE34 pKa = 4.21 VSEE37 pKa = 4.19 IRR39 pKa = 11.84 DD40 pKa = 3.7 GNPVLRR46 pKa = 11.84 AAEE49 pKa = 4.63 EE50 pKa = 3.86 IRR52 pKa = 11.84 EE53 pKa = 4.23 DD54 pKa = 3.17 WGEE57 pKa = 3.71
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.91
IPC_protein 3.783
Toseland 3.617
ProMoST 3.872
Dawson 3.745
Bjellqvist 3.986
Wikipedia 3.643
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 3.973
Thurlkill 3.656
EMBOSS 3.656
Sillero 3.897
Patrickios 1.85
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|T0LVU9|T0LVU9_9ARCH Metallophos domain-containing protein OS=Thermoplasmatales archaeon Gpl OX=261391 GN=AMDU5_GPLC00010G0014 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.48 GIAGRR7 pKa = 11.84 QSRR10 pKa = 11.84 GLPLGARR17 pKa = 11.84 MVCADD22 pKa = 3.43 NSGAKK27 pKa = 9.25 IVSLINVKK35 pKa = 9.65 AWHH38 pKa = 5.66 GVARR42 pKa = 11.84 RR43 pKa = 11.84 VPAAGVGDD51 pKa = 3.8 MFIASVKK58 pKa = 10.55 KK59 pKa = 10.43 GGPDD63 pKa = 3.03 VRR65 pKa = 11.84 AKK67 pKa = 10.3 VVYY70 pKa = 10.31 AVVIRR75 pKa = 11.84 QKK77 pKa = 10.56 RR78 pKa = 11.84 PYY80 pKa = 9.65 RR81 pKa = 11.84 RR82 pKa = 11.84 ADD84 pKa = 3.16 GSIISFEE91 pKa = 4.09 EE92 pKa = 3.73 NAAVLVTPDD101 pKa = 3.33 GEE103 pKa = 4.6 VRR105 pKa = 11.84 GSEE108 pKa = 3.61 IKK110 pKa = 11.02 GPVARR115 pKa = 11.84 EE116 pKa = 3.52 AAEE119 pKa = 3.75 RR120 pKa = 11.84 WPRR123 pKa = 11.84 IAAIASTIVV132 pKa = 2.82
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.677
IPC_protein 10.526
Toseland 10.935
ProMoST 10.672
Dawson 10.979
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.184
Grimsley 11.008
Solomon 11.169
Lehninger 11.125
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.906
EMBOSS 11.345
Sillero 10.921
Patrickios 10.921
IPC_peptide 11.169
IPC2_peptide 9.633
IPC2.peptide.svr19 8.741
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1917
0
1917
536142
22
1911
279.7
31.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.367 ± 0.053
0.694 ± 0.02
5.118 ± 0.053
6.894 ± 0.077
4.877 ± 0.05
6.988 ± 0.051
1.518 ± 0.02
9.116 ± 0.055
7.104 ± 0.069
8.892 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.086 ± 0.033
5.334 ± 0.053
3.543 ± 0.032
2.338 ± 0.027
4.711 ± 0.043
7.944 ± 0.06
5.112 ± 0.043
6.426 ± 0.041
0.866 ± 0.018
4.067 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here