Xenorhabdus szentirmaii DSM 16338
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4565 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W1IWR3|W1IWR3_9GAMM HpaG2 protein OS=Xenorhabdus szentirmaii DSM 16338 OX=1427518 GN=XSR1_260021 PE=4 SV=1
MM1 pKa = 7.49 AKK3 pKa = 9.87 IGIFVGTVYY12 pKa = 11.17 GNALAVAEE20 pKa = 4.42 EE21 pKa = 4.12 ALQILTQQGHH31 pKa = 4.14 QVEE34 pKa = 4.44 IFEE37 pKa = 5.48 DD38 pKa = 3.43 ATLEE42 pKa = 3.84 QWQEE46 pKa = 3.75 YY47 pKa = 8.39 LQDD50 pKa = 3.85 VILVVTSTTGNGDD63 pKa = 3.72 LPDD66 pKa = 5.15 GIEE69 pKa = 4.38 PLYY72 pKa = 10.83 TDD74 pKa = 5.22 LNDD77 pKa = 3.75 KK78 pKa = 10.79 LGYY81 pKa = 10.2 QPDD84 pKa = 3.83 LRR86 pKa = 11.84 YY87 pKa = 10.94 GMIVLGDD94 pKa = 3.54 SSYY97 pKa = 11.18 DD98 pKa = 3.6 TFCGGGRR105 pKa = 11.84 AFDD108 pKa = 4.4 EE109 pKa = 4.35 LLQEE113 pKa = 4.14 QGAKK117 pKa = 10.33 RR118 pKa = 11.84 IGDD121 pKa = 3.8 MLLIDD126 pKa = 5.6 AIDD129 pKa = 3.59 VTEE132 pKa = 4.44 PEE134 pKa = 4.75 IFAQDD139 pKa = 3.69 WIEE142 pKa = 3.92 GWGTLLL148 pKa = 4.92
Molecular weight: 16.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.516
EMBOSS 3.592
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|W1J3U8|W1J3U8_9GAMM Cell division protein ZapB OS=Xenorhabdus szentirmaii DSM 16338 OX=1427518 GN=zapB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4565
0
4565
1315782
16
4487
288.2
32.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.028 ± 0.038
1.112 ± 0.014
5.169 ± 0.031
6.057 ± 0.036
4.063 ± 0.029
6.813 ± 0.044
2.466 ± 0.022
6.913 ± 0.042
5.262 ± 0.037
10.505 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.626 ± 0.02
4.531 ± 0.033
4.245 ± 0.028
4.568 ± 0.036
5.066 ± 0.034
6.48 ± 0.028
5.272 ± 0.026
6.218 ± 0.03
1.368 ± 0.015
3.238 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here