Mycobacterium phage Amgine

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZMH3|A0A222ZMH3_9CAUD Uncharacterized protein OS=Mycobacterium phage Amgine OX=2015817 GN=78 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84APSTVEE8 pKa = 4.21VPCPACGEE16 pKa = 4.36PIEE19 pKa = 4.79LSLGFEE25 pKa = 4.13SVEE28 pKa = 3.94PVEE31 pKa = 4.38GLNVASFVVTTPDD44 pKa = 3.29LQDD47 pKa = 3.3RR48 pKa = 11.84AQSHH52 pKa = 6.39GEE54 pKa = 3.93VCPVLTGGGRR64 pKa = 11.84DD65 pKa = 3.43EE66 pKa = 4.3

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZNF8|A0A222ZNF8_9CAUD Uncharacterized protein OS=Mycobacterium phage Amgine OX=2015817 GN=91 PE=4 SV=1
MM1 pKa = 7.49VILKK5 pKa = 7.16TAPWPFYY12 pKa = 8.73HH13 pKa = 6.25QRR15 pKa = 11.84RR16 pKa = 11.84AARR19 pKa = 11.84LLGLRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.05IGGLVARR34 pKa = 11.84GSLRR38 pKa = 11.84GVRR41 pKa = 11.84VHH43 pKa = 7.29DD44 pKa = 4.48GAGGWVWAVNCHH56 pKa = 6.95DD57 pKa = 4.55VDD59 pKa = 4.02TLAASPNYY67 pKa = 9.63RR68 pKa = 11.84HH69 pKa = 6.49ARR71 pKa = 11.84DD72 pKa = 3.51IPDD75 pKa = 2.86IRR77 pKa = 11.84MLRR80 pKa = 11.84AIDD83 pKa = 5.32DD84 pKa = 3.85ATQPWGNALRR94 pKa = 11.84GDD96 pKa = 3.79VTRR99 pKa = 11.84VLGGVPLDD107 pKa = 3.62SQLARR112 pKa = 11.84GEE114 pKa = 4.4VPGVPWKK121 pKa = 10.88VVLAKK126 pKa = 10.03FRR128 pKa = 11.84RR129 pKa = 11.84VAGRR133 pKa = 11.84GLADD137 pKa = 4.33GCDD140 pKa = 3.67CGCRR144 pKa = 11.84GDD146 pKa = 3.92WSLTDD151 pKa = 3.2KK152 pKa = 11.07GRR154 pKa = 11.84ALVEE158 pKa = 3.67LSRR161 pKa = 11.84CVDD164 pKa = 3.68APCYY168 pKa = 9.98RR169 pKa = 11.84HH170 pKa = 6.84AEE172 pKa = 4.15HH173 pKa = 7.5ADD175 pKa = 3.51RR176 pKa = 11.84AARR179 pKa = 11.84RR180 pKa = 11.84PGLRR184 pKa = 11.84AVLRR188 pKa = 4.17

Molecular weight: 20.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

19723

38

1382

203.3

22.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.975 ± 0.467

1.247 ± 0.155

6.525 ± 0.257

5.623 ± 0.301

2.682 ± 0.148

8.827 ± 0.492

2.256 ± 0.198

3.711 ± 0.197

3.564 ± 0.184

8.452 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.1

2.855 ± 0.201

5.557 ± 0.226

3.286 ± 0.147

7.311 ± 0.38

5.141 ± 0.255

5.871 ± 0.218

7.752 ± 0.262

2.074 ± 0.113

2.185 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski