Amino acid dipepetide frequency for Mycobacterium phage Amgine

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.107AlaAla: 22.107 ± 1.594
1.166AlaCys: 1.166 ± 0.222
8.468AlaAsp: 8.468 ± 0.603
9.786AlaGlu: 9.786 ± 0.903
3.296AlaPhe: 3.296 ± 0.54
8.873AlaGly: 8.873 ± 1.007
2.687AlaHis: 2.687 ± 0.367
4.969AlaIle: 4.969 ± 0.546
3.854AlaLys: 3.854 ± 0.379
11.662AlaLeu: 11.662 ± 0.726
3.701AlaMet: 3.701 ± 0.514
3.042AlaAsn: 3.042 ± 0.447
6.287AlaPro: 6.287 ± 0.659
4.969AlaGln: 4.969 ± 0.613
9.076AlaArg: 9.076 ± 0.75
6.034AlaSer: 6.034 ± 0.584
6.693AlaThr: 6.693 ± 0.668
8.772AlaVal: 8.772 ± 0.731
2.18AlaTrp: 2.18 ± 0.293
2.13AlaTyr: 2.13 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.913CysAla: 0.913 ± 0.192
0.101CysCys: 0.101 ± 0.077
0.608CysAsp: 0.608 ± 0.176
0.862CysGlu: 0.862 ± 0.21
0.203CysPhe: 0.203 ± 0.102
1.876CysGly: 1.876 ± 0.32
0.304CysHis: 0.304 ± 0.166
0.406CysIle: 0.406 ± 0.132
0.558CysLys: 0.558 ± 0.139
0.761CysLeu: 0.761 ± 0.156
0.203CysMet: 0.203 ± 0.103
0.507CysAsn: 0.507 ± 0.169
1.065CysPro: 1.065 ± 0.247
0.304CysGln: 0.304 ± 0.128
0.862CysArg: 0.862 ± 0.206
1.065CysSer: 1.065 ± 0.276
0.608CysThr: 0.608 ± 0.163
0.71CysVal: 0.71 ± 0.232
0.406CysTrp: 0.406 ± 0.154
0.152CysTyr: 0.152 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
7.454AspAla: 7.454 ± 0.623
1.116AspCys: 1.116 ± 0.249
5.425AspAsp: 5.425 ± 0.683
5.527AspGlu: 5.527 ± 0.661
0.963AspPhe: 0.963 ± 0.274
6.085AspGly: 6.085 ± 0.457
1.268AspHis: 1.268 ± 0.271
1.876AspIle: 1.876 ± 0.328
2.18AspLys: 2.18 ± 0.368
6.034AspLeu: 6.034 ± 0.619
1.065AspMet: 1.065 ± 0.228
1.927AspAsn: 1.927 ± 0.286
3.854AspPro: 3.854 ± 0.468
2.231AspGln: 2.231 ± 0.271
5.425AspArg: 5.425 ± 0.607
2.789AspSer: 2.789 ± 0.312
3.042AspThr: 3.042 ± 0.396
4.614AspVal: 4.614 ± 0.426
1.116AspTrp: 1.116 ± 0.205
1.268AspTyr: 1.268 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
8.366GluAla: 8.366 ± 0.743
0.862GluCys: 0.862 ± 0.209
3.093GluAsp: 3.093 ± 0.534
0.811GluGlu: 0.811 ± 0.213
1.572GluPhe: 1.572 ± 0.304
3.701GluGly: 3.701 ± 0.503
1.42GluHis: 1.42 ± 0.277
1.572GluIle: 1.572 ± 0.313
2.079GluLys: 2.079 ± 0.436
4.817GluLeu: 4.817 ± 0.529
1.521GluMet: 1.521 ± 0.235
1.217GluAsn: 1.217 ± 0.229
3.042GluPro: 3.042 ± 0.441
3.296GluGln: 3.296 ± 0.426
4.969GluArg: 4.969 ± 0.623
2.789GluSer: 2.789 ± 0.397
2.535GluThr: 2.535 ± 0.32
5.78GluVal: 5.78 ± 0.582
1.166GluTrp: 1.166 ± 0.256
1.623GluTyr: 1.623 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
2.738PheAla: 2.738 ± 0.366
0.355PheCys: 0.355 ± 0.126
2.839PheAsp: 2.839 ± 0.351
1.369PheGlu: 1.369 ± 0.256
0.456PhePhe: 0.456 ± 0.15
3.245PheGly: 3.245 ± 0.451
0.254PheHis: 0.254 ± 0.127
0.71PheIle: 0.71 ± 0.197
1.014PheLys: 1.014 ± 0.255
2.028PheLeu: 2.028 ± 0.314
0.507PheMet: 0.507 ± 0.142
1.623PheAsn: 1.623 ± 0.386
1.116PhePro: 1.116 ± 0.185
0.558PheGln: 0.558 ± 0.162
1.673PheArg: 1.673 ± 0.332
0.761PheSer: 0.761 ± 0.19
1.825PheThr: 1.825 ± 0.279
2.687PheVal: 2.687 ± 0.286
0.507PheTrp: 0.507 ± 0.134
0.558PheTyr: 0.558 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
9.837GlyAla: 9.837 ± 0.865
1.268GlyCys: 1.268 ± 0.339
5.273GlyAsp: 5.273 ± 0.483
5.425GlyGlu: 5.425 ± 0.497
2.18GlyPhe: 2.18 ± 0.335
9.533GlyGly: 9.533 ± 1.311
1.623GlyHis: 1.623 ± 0.342
3.042GlyIle: 3.042 ± 0.525
4.614GlyLys: 4.614 ± 0.464
6.693GlyLeu: 6.693 ± 0.704
1.47GlyMet: 1.47 ± 0.245
3.296GlyAsn: 3.296 ± 0.569
3.752GlyPro: 3.752 ± 0.573
2.383GlyGln: 2.383 ± 0.493
5.983GlyArg: 5.983 ± 0.579
4.868GlySer: 4.868 ± 0.638
5.476GlyThr: 5.476 ± 0.523
7.099GlyVal: 7.099 ± 0.544
2.586GlyTrp: 2.586 ± 0.357
1.876GlyTyr: 1.876 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
2.282HisAla: 2.282 ± 0.423
0.659HisCys: 0.659 ± 0.188
1.116HisAsp: 1.116 ± 0.3
0.913HisGlu: 0.913 ± 0.255
0.507HisPhe: 0.507 ± 0.156
2.079HisGly: 2.079 ± 0.306
0.608HisHis: 0.608 ± 0.157
1.014HisIle: 1.014 ± 0.246
0.558HisLys: 0.558 ± 0.155
1.927HisLeu: 1.927 ± 0.324
0.507HisMet: 0.507 ± 0.161
0.608HisAsn: 0.608 ± 0.148
1.42HisPro: 1.42 ± 0.319
0.761HisGln: 0.761 ± 0.219
1.623HisArg: 1.623 ± 0.293
0.862HisSer: 0.862 ± 0.211
1.521HisThr: 1.521 ± 0.266
2.079HisVal: 2.079 ± 0.327
0.558HisTrp: 0.558 ± 0.222
0.963HisTyr: 0.963 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
5.78IleAla: 5.78 ± 0.528
0.051IleCys: 0.051 ± 0.045
3.752IleAsp: 3.752 ± 0.435
3.042IleGlu: 3.042 ± 0.427
1.065IlePhe: 1.065 ± 0.254
4.107IleGly: 4.107 ± 0.804
0.304IleHis: 0.304 ± 0.118
0.811IleIle: 0.811 ± 0.234
1.521IleLys: 1.521 ± 0.257
2.332IleLeu: 2.332 ± 0.332
0.304IleMet: 0.304 ± 0.122
1.065IleAsn: 1.065 ± 0.233
1.673IlePro: 1.673 ± 0.334
0.761IleGln: 0.761 ± 0.163
2.535IleArg: 2.535 ± 0.382
1.268IleSer: 1.268 ± 0.298
2.332IleThr: 2.332 ± 0.358
3.194IleVal: 3.194 ± 0.379
0.862IleTrp: 0.862 ± 0.197
0.355IleTyr: 0.355 ± 0.13
0.0IleXaa: 0.0 ± 0.0
Lys
4.614LysAla: 4.614 ± 0.577
0.608LysCys: 0.608 ± 0.154
1.47LysAsp: 1.47 ± 0.257
0.761LysGlu: 0.761 ± 0.167
1.116LysPhe: 1.116 ± 0.227
3.6LysGly: 3.6 ± 0.518
0.963LysHis: 0.963 ± 0.281
1.977LysIle: 1.977 ± 0.349
0.659LysLys: 0.659 ± 0.172
3.701LysLeu: 3.701 ± 0.421
0.811LysMet: 0.811 ± 0.205
0.456LysAsn: 0.456 ± 0.144
3.144LysPro: 3.144 ± 0.429
0.862LysGln: 0.862 ± 0.184
2.789LysArg: 2.789 ± 0.491
1.014LysSer: 1.014 ± 0.216
2.231LysThr: 2.231 ± 0.349
3.093LysVal: 3.093 ± 0.407
0.659LysTrp: 0.659 ± 0.16
1.065LysTyr: 1.065 ± 0.293
0.0LysXaa: 0.0 ± 0.0
Leu
12.524LeuAla: 12.524 ± 0.882
0.862LeuCys: 0.862 ± 0.257
7.301LeuAsp: 7.301 ± 0.567
2.18LeuGlu: 2.18 ± 0.326
2.434LeuPhe: 2.434 ± 0.396
6.085LeuGly: 6.085 ± 0.531
1.825LeuHis: 1.825 ± 0.266
3.752LeuIle: 3.752 ± 0.433
2.839LeuLys: 2.839 ± 0.371
5.73LeuLeu: 5.73 ± 0.615
1.977LeuMet: 1.977 ± 0.362
2.079LeuAsn: 2.079 ± 0.277
4.716LeuPro: 4.716 ± 0.528
3.042LeuGln: 3.042 ± 0.491
6.389LeuArg: 6.389 ± 0.661
5.78LeuSer: 5.78 ± 0.438
5.223LeuThr: 5.223 ± 0.496
6.642LeuVal: 6.642 ± 0.563
1.42LeuTrp: 1.42 ± 0.339
1.724LeuTyr: 1.724 ± 0.318
0.0LeuXaa: 0.0 ± 0.0
Met
2.332MetAla: 2.332 ± 0.309
0.152MetCys: 0.152 ± 0.083
1.014MetAsp: 1.014 ± 0.236
0.456MetGlu: 0.456 ± 0.138
0.71MetPhe: 0.71 ± 0.205
1.318MetGly: 1.318 ± 0.272
0.811MetHis: 0.811 ± 0.184
1.014MetIle: 1.014 ± 0.211
0.507MetLys: 0.507 ± 0.147
1.521MetLeu: 1.521 ± 0.3
0.406MetMet: 0.406 ± 0.163
0.71MetAsn: 0.71 ± 0.188
1.217MetPro: 1.217 ± 0.306
0.71MetGln: 0.71 ± 0.174
1.876MetArg: 1.876 ± 0.316
2.383MetSer: 2.383 ± 0.28
1.673MetThr: 1.673 ± 0.255
1.47MetVal: 1.47 ± 0.263
0.304MetTrp: 0.304 ± 0.116
0.456MetTyr: 0.456 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.955AsnAla: 3.955 ± 0.632
0.456AsnCys: 0.456 ± 0.161
1.623AsnAsp: 1.623 ± 0.34
1.42AsnGlu: 1.42 ± 0.25
0.811AsnPhe: 0.811 ± 0.201
3.752AsnGly: 3.752 ± 0.583
0.304AsnHis: 0.304 ± 0.124
0.761AsnIle: 0.761 ± 0.214
0.913AsnLys: 0.913 ± 0.185
1.927AsnLeu: 1.927 ± 0.337
0.608AsnMet: 0.608 ± 0.154
0.71AsnAsn: 0.71 ± 0.193
2.332AsnPro: 2.332 ± 0.319
0.761AsnGln: 0.761 ± 0.266
1.47AsnArg: 1.47 ± 0.263
1.268AsnSer: 1.268 ± 0.272
2.13AsnThr: 2.13 ± 0.377
2.434AsnVal: 2.434 ± 0.338
0.304AsnTrp: 0.304 ± 0.115
0.608AsnTyr: 0.608 ± 0.123
0.0AsnXaa: 0.0 ± 0.0
Pro
7.454ProAla: 7.454 ± 0.719
0.507ProCys: 0.507 ± 0.164
3.144ProAsp: 3.144 ± 0.427
4.361ProGlu: 4.361 ± 0.454
1.927ProPhe: 1.927 ± 0.317
5.476ProGly: 5.476 ± 0.48
1.572ProHis: 1.572 ± 0.292
1.673ProIle: 1.673 ± 0.283
2.383ProLys: 2.383 ± 0.36
4.513ProLeu: 4.513 ± 0.487
1.065ProMet: 1.065 ± 0.293
1.369ProAsn: 1.369 ± 0.285
3.042ProPro: 3.042 ± 0.416
1.217ProGln: 1.217 ± 0.268
3.296ProArg: 3.296 ± 0.46
3.042ProSer: 3.042 ± 0.491
3.701ProThr: 3.701 ± 0.453
3.6ProVal: 3.6 ± 0.427
0.862ProTrp: 0.862 ± 0.24
1.369ProTyr: 1.369 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
4.918GlnAla: 4.918 ± 0.609
0.304GlnCys: 0.304 ± 0.148
0.862GlnAsp: 0.862 ± 0.187
1.318GlnGlu: 1.318 ± 0.245
0.761GlnPhe: 0.761 ± 0.272
2.535GlnGly: 2.535 ± 0.304
1.116GlnHis: 1.116 ± 0.218
1.775GlnIle: 1.775 ± 0.217
1.065GlnLys: 1.065 ± 0.251
2.789GlnLeu: 2.789 ± 0.412
0.761GlnMet: 0.761 ± 0.194
0.406GlnAsn: 0.406 ± 0.124
2.231GlnPro: 2.231 ± 0.278
1.318GlnGln: 1.318 ± 0.283
2.992GlnArg: 2.992 ± 0.311
1.623GlnSer: 1.623 ± 0.353
2.028GlnThr: 2.028 ± 0.355
2.434GlnVal: 2.434 ± 0.375
0.761GlnTrp: 0.761 ± 0.163
0.862GlnTyr: 0.862 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
7.504ArgAla: 7.504 ± 0.762
1.318ArgCys: 1.318 ± 0.268
4.665ArgAsp: 4.665 ± 0.446
3.904ArgGlu: 3.904 ± 0.478
1.876ArgPhe: 1.876 ± 0.328
5.578ArgGly: 5.578 ± 0.613
1.623ArgHis: 1.623 ± 0.339
2.941ArgIle: 2.941 ± 0.406
3.549ArgLys: 3.549 ± 0.407
7.352ArgLeu: 7.352 ± 0.665
1.825ArgMet: 1.825 ± 0.29
2.839ArgAsn: 2.839 ± 0.408
3.651ArgPro: 3.651 ± 0.529
2.282ArgGln: 2.282 ± 0.328
6.034ArgArg: 6.034 ± 0.629
3.549ArgSer: 3.549 ± 0.373
4.056ArgThr: 4.056 ± 0.544
5.172ArgVal: 5.172 ± 0.481
1.927ArgTrp: 1.927 ± 0.32
1.47ArgTyr: 1.47 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
7.048SerAla: 7.048 ± 0.728
0.456SerCys: 0.456 ± 0.183
2.586SerAsp: 2.586 ± 0.287
2.839SerGlu: 2.839 ± 0.335
1.572SerPhe: 1.572 ± 0.289
4.766SerGly: 4.766 ± 0.511
1.47SerHis: 1.47 ± 0.294
2.485SerIle: 2.485 ± 0.404
1.521SerLys: 1.521 ± 0.325
4.462SerLeu: 4.462 ± 0.403
1.166SerMet: 1.166 ± 0.191
1.369SerAsn: 1.369 ± 0.234
2.992SerPro: 2.992 ± 0.361
1.825SerGln: 1.825 ± 0.274
2.637SerArg: 2.637 ± 0.354
2.941SerSer: 2.941 ± 0.452
2.89SerThr: 2.89 ± 0.31
4.107SerVal: 4.107 ± 0.412
1.318SerTrp: 1.318 ± 0.285
0.963SerTyr: 0.963 ± 0.206
0.0SerXaa: 0.0 ± 0.0
Thr
6.287ThrAla: 6.287 ± 0.651
0.71ThrCys: 0.71 ± 0.195
2.89ThrAsp: 2.89 ± 0.406
3.6ThrGlu: 3.6 ± 0.329
2.282ThrPhe: 2.282 ± 0.255
5.527ThrGly: 5.527 ± 0.564
1.825ThrHis: 1.825 ± 0.387
2.535ThrIle: 2.535 ± 0.469
2.332ThrLys: 2.332 ± 0.34
4.918ThrLeu: 4.918 ± 0.463
1.014ThrMet: 1.014 ± 0.224
1.724ThrAsn: 1.724 ± 0.281
3.752ThrPro: 3.752 ± 0.491
1.724ThrGln: 1.724 ± 0.274
4.056ThrArg: 4.056 ± 0.487
2.89ThrSer: 2.89 ± 0.452
3.245ThrThr: 3.245 ± 0.375
4.462ThrVal: 4.462 ± 0.445
1.014ThrTrp: 1.014 ± 0.185
1.927ThrTyr: 1.927 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
8.772ValAla: 8.772 ± 0.786
0.963ValCys: 0.963 ± 0.209
6.693ValAsp: 6.693 ± 0.678
5.527ValGlu: 5.527 ± 0.75
2.18ValPhe: 2.18 ± 0.405
6.693ValGly: 6.693 ± 0.625
1.521ValHis: 1.521 ± 0.295
2.485ValIle: 2.485 ± 0.404
2.637ValLys: 2.637 ± 0.297
6.693ValLeu: 6.693 ± 0.568
1.369ValMet: 1.369 ± 0.195
2.485ValAsn: 2.485 ± 0.361
4.361ValPro: 4.361 ± 0.44
2.18ValGln: 2.18 ± 0.348
4.918ValArg: 4.918 ± 0.643
3.904ValSer: 3.904 ± 0.402
4.563ValThr: 4.563 ± 0.409
6.135ValVal: 6.135 ± 0.619
1.977ValTrp: 1.977 ± 0.316
1.47ValTyr: 1.47 ± 0.246
0.0ValXaa: 0.0 ± 0.0
Trp
2.231TrpAla: 2.231 ± 0.293
0.355TrpCys: 0.355 ± 0.137
1.014TrpAsp: 1.014 ± 0.226
0.608TrpGlu: 0.608 ± 0.147
0.507TrpPhe: 0.507 ± 0.151
1.166TrpGly: 1.166 ± 0.232
0.761TrpHis: 0.761 ± 0.178
0.862TrpIle: 0.862 ± 0.178
0.304TrpLys: 0.304 ± 0.12
2.535TrpLeu: 2.535 ± 0.357
0.254TrpMet: 0.254 ± 0.095
0.507TrpAsn: 0.507 ± 0.173
0.963TrpPro: 0.963 ± 0.207
1.065TrpGln: 1.065 ± 0.3
2.586TrpArg: 2.586 ± 0.446
1.166TrpSer: 1.166 ± 0.263
1.521TrpThr: 1.521 ± 0.277
1.521TrpVal: 1.521 ± 0.322
0.355TrpTrp: 0.355 ± 0.134
0.456TrpTyr: 0.456 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.637TyrAla: 2.637 ± 0.304
0.203TyrCys: 0.203 ± 0.092
1.42TyrAsp: 1.42 ± 0.283
1.521TyrGlu: 1.521 ± 0.289
0.406TyrPhe: 0.406 ± 0.136
2.282TyrGly: 2.282 ± 0.359
0.304TyrHis: 0.304 ± 0.136
0.456TyrIle: 0.456 ± 0.157
0.558TyrLys: 0.558 ± 0.179
2.13TyrLeu: 2.13 ± 0.376
0.456TyrMet: 0.456 ± 0.16
0.608TyrAsn: 0.608 ± 0.136
0.963TyrPro: 0.963 ± 0.23
0.608TyrGln: 0.608 ± 0.185
1.927TyrArg: 1.927 ± 0.302
1.42TyrSer: 1.42 ± 0.285
1.42TyrThr: 1.42 ± 0.227
1.521TyrVal: 1.521 ± 0.237
0.456TyrTrp: 0.456 ± 0.181
0.558TyrTyr: 0.558 ± 0.15
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (19723 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski