Crocosphaera subtropica (strain ATCC 51142 / BH68) (Cyanothece sp. (strain ATCC 51142))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Crocosphaera; Crocosphaera subtropica

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1WQG7|B1WQG7_CROS5 Putative Fe-S metabolism associated SufE OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=cce_2328 PE=3 SV=1
MM1 pKa = 7.47IFKK4 pKa = 10.51SSNCMGICQQKK15 pKa = 9.06WLTTLSLGIIFSLSGAISAKK35 pKa = 10.06AATVSYY41 pKa = 9.23EE42 pKa = 3.99YY43 pKa = 10.54EE44 pKa = 3.91PASIGGNDD52 pKa = 3.79NDD54 pKa = 4.59PFLDD58 pKa = 3.3IAGVSGGFATLGYY71 pKa = 9.65QFSVNPDD78 pKa = 3.34PTDD81 pKa = 3.29VNLEE85 pKa = 3.84MTAFGVYY92 pKa = 10.47ASRR95 pKa = 11.84TNIIEE100 pKa = 4.22PNNPEE105 pKa = 4.68DD106 pKa = 4.68FFLLPEE112 pKa = 4.02QQTCFTLTGQTCFTLPEE129 pKa = 4.32TVHH132 pKa = 6.63EE133 pKa = 4.31VALWEE138 pKa = 4.13VDD140 pKa = 4.02SNGNPLNPAPVRR152 pKa = 11.84KK153 pKa = 8.52ITIAPTIDD161 pKa = 3.92DD162 pKa = 4.57PNNPGTSITNPLLAFVDD179 pKa = 4.02DD180 pKa = 4.8FAYY183 pKa = 10.67LALDD187 pKa = 4.94DD188 pKa = 5.44PDD190 pKa = 5.99DD191 pKa = 5.57PITPDD196 pKa = 5.27DD197 pKa = 4.36EE198 pKa = 6.8LDD200 pKa = 5.47DD201 pKa = 4.26ILTLSGSQQFFRR213 pKa = 11.84LGVTYY218 pKa = 10.51TDD220 pKa = 4.2DD221 pKa = 3.47TDD223 pKa = 3.93QAWVSAFTGGSVVVPNEE240 pKa = 4.28DD241 pKa = 3.48LDD243 pKa = 4.11GVPNEE248 pKa = 4.4GAAVDD253 pKa = 4.45PMLQLLPDD261 pKa = 3.95NPGQGYY267 pKa = 10.2YY268 pKa = 10.65GIPSDD273 pKa = 5.1PMTDD277 pKa = 3.17PLAFPDD283 pKa = 3.69QTTLPFFGTSNPFFVAGNILFGPLPPGFPVAATTTGTGTSTGTGTSTGTGTSTGTSTGTGTSTGTSTGTGTSTGTGTSTGTSTGTGGVPTIPEE376 pKa = 4.15PNLIHH381 pKa = 6.71GLLAITLGGIFTRR394 pKa = 11.84YY395 pKa = 8.21NHH397 pKa = 5.85GKK399 pKa = 6.87TRR401 pKa = 11.84RR402 pKa = 3.5

Molecular weight: 41.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1WWL6|B1WWL6_CROS5 Uncharacterized protein OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=cce_1390 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 8.99RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84KK25 pKa = 9.23SNGRR29 pKa = 11.84KK30 pKa = 9.11VIQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.98KK38 pKa = 9.65GRR40 pKa = 11.84HH41 pKa = 5.0RR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5239

0

5239

1562895

17

3193

298.3

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.575 ± 0.04

1.0 ± 0.012

5.078 ± 0.031

6.621 ± 0.035

4.141 ± 0.026

6.434 ± 0.039

1.897 ± 0.018

7.509 ± 0.032

5.574 ± 0.037

11.073 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.016

4.927 ± 0.037

4.527 ± 0.023

5.208 ± 0.031

4.505 ± 0.028

6.378 ± 0.024

5.766 ± 0.03

6.083 ± 0.028

1.424 ± 0.015

3.288 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski