Xenococcus sp. PCC 7305
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5347 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L8M053|L8M053_9CYAN Uncharacterized protein OS=Xenococcus sp. PCC 7305 OX=102125 GN=Xen7305DRAFT_00004360 PE=4 SV=1
MM1 pKa = 7.78 AEE3 pKa = 4.33 NLIMWIKK10 pKa = 10.49 QLGSPEE16 pKa = 4.21 NDD18 pKa = 2.98 LSQGIAIDD26 pKa = 3.66 SNGNVFISGSTRR38 pKa = 11.84 GTLGNTNFGNSDD50 pKa = 3.24 TWVAKK55 pKa = 10.27 YY56 pKa = 10.29 DD57 pKa = 3.68 HH58 pKa = 6.6 QGNLLWTQQFGTSNLDD74 pKa = 3.45 DD75 pKa = 4.4 FEE77 pKa = 6.4 GLATDD82 pKa = 3.86 INGHH86 pKa = 5.21 VYY88 pKa = 10.16 IAGSSLGTIEE98 pKa = 4.25 GTFQIDD104 pKa = 2.6 SDD106 pKa = 3.77 AWVNKK111 pKa = 10.15 YY112 pKa = 10.33 DD113 pKa = 4.06 GQGNLLWSQQLGFSGFDD130 pKa = 3.13 SATGVATDD138 pKa = 4.22 LGGNVYY144 pKa = 10.34 ISGFTNGISDD154 pKa = 3.75 EE155 pKa = 4.4 ANGGGSVAWVTKK167 pKa = 10.75 YY168 pKa = 10.62 DD169 pKa = 3.66 VQGNLLWNQQLDD181 pKa = 3.63 ASGSEE186 pKa = 3.61 ISAGVTTDD194 pKa = 2.43 IHH196 pKa = 6.79 GNFYY200 pKa = 10.56 ISGDD204 pKa = 3.68 TTRR207 pKa = 11.84 SNSEE211 pKa = 3.66 NTNAWVAKK219 pKa = 9.92 YY220 pKa = 9.99 DD221 pKa = 4.29 AEE223 pKa = 5.91 GNLLWNQQLDD233 pKa = 3.8 SAEE236 pKa = 4.34 AEE238 pKa = 3.95 FDD240 pKa = 3.44 YY241 pKa = 11.2 SQAVTTDD248 pKa = 3.29 LSGNVYY254 pKa = 10.18 IAGGTNGSIDD264 pKa = 3.64 RR265 pKa = 11.84 GNAGLNDD272 pKa = 3.44 AWVAKK277 pKa = 10.53 YY278 pKa = 10.48 DD279 pKa = 4.28 GEE281 pKa = 5.22 GNLLWTEE288 pKa = 3.9 QLGTVGNDD296 pKa = 2.85 AAEE299 pKa = 5.22 GITTDD304 pKa = 3.7 SEE306 pKa = 4.78 GNVYY310 pKa = 10.33 ISGFTNEE317 pKa = 4.45 TLGEE321 pKa = 4.13 ANAGGSDD328 pKa = 3.21 AWVAKK333 pKa = 10.31 YY334 pKa = 10.59 DD335 pKa = 4.3 SNGNLLWTEE344 pKa = 4.01 QFGSPEE350 pKa = 4.52 DD351 pKa = 4.62 DD352 pKa = 4.11 DD353 pKa = 4.64 SQGVAIDD360 pKa = 4.28 SNGSLYY366 pKa = 10.9 LSGSTEE372 pKa = 4.07 GNLDD376 pKa = 3.36 GVNFGGEE383 pKa = 4.4 DD384 pKa = 2.94 AWVAQISQPIIPDD397 pKa = 3.17 NGGNGGNNGNGGGSAPLLTTPINRR421 pKa = 11.84 FQNNSLPGTYY431 pKa = 10.26 LFAGEE436 pKa = 5.27 DD437 pKa = 3.62 EE438 pKa = 4.63 SQGIRR443 pKa = 11.84 ANFPNFVEE451 pKa = 4.28 EE452 pKa = 4.42 GQAFRR457 pKa = 11.84 VGVEE461 pKa = 4.07 PGDD464 pKa = 3.37 NLIRR468 pKa = 11.84 LNRR471 pKa = 11.84 FQNSNVPGTYY481 pKa = 9.86 LYY483 pKa = 10.85 AGEE486 pKa = 4.96 EE487 pKa = 4.24 EE488 pKa = 4.52 SQSIRR493 pKa = 11.84 ANFSNFIEE501 pKa = 4.29 EE502 pKa = 4.7 GIAFYY507 pKa = 10.94 VYY509 pKa = 10.78 AGTADD514 pKa = 3.12 IGTDD518 pKa = 3.66 FYY520 pKa = 11.4 RR521 pKa = 11.84 FQNQNVPGTYY531 pKa = 9.43 IFVGGNEE538 pKa = 4.11 RR539 pKa = 11.84 QNILANFPHH548 pKa = 6.09 FHH550 pKa = 6.78 EE551 pKa = 4.79 EE552 pKa = 4.45 GIAFEE557 pKa = 4.46 VGTT560 pKa = 4.42
Molecular weight: 59.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 1.341
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|L8LYW6|L8LYW6_9CYAN Uncharacterized protein OS=Xenococcus sp. PCC 7305 OX=102125 GN=Xen7305DRAFT_00009040 PE=4 SV=1
MM1 pKa = 7.55 TKK3 pKa = 9.01 RR4 pKa = 11.84 TLGGTSRR11 pKa = 11.84 KK12 pKa = 7.83 QKK14 pKa = 9.77 RR15 pKa = 11.84 KK16 pKa = 8.61 SGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TKK26 pKa = 10.37 NGRR29 pKa = 11.84 KK30 pKa = 9.19 VIQARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.98 KK38 pKa = 9.65 GRR40 pKa = 11.84 HH41 pKa = 5.0 RR42 pKa = 11.84 LSVV45 pKa = 3.12
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5347
0
5347
1695650
20
10636
317.1
35.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.621 ± 0.036
0.928 ± 0.014
5.477 ± 0.053
6.55 ± 0.033
4.161 ± 0.025
6.652 ± 0.054
1.682 ± 0.018
7.544 ± 0.029
5.289 ± 0.044
10.763 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.719 ± 0.021
5.029 ± 0.036
4.196 ± 0.024
5.055 ± 0.035
4.518 ± 0.031
6.711 ± 0.026
5.527 ± 0.038
6.074 ± 0.029
1.34 ± 0.015
3.162 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here