Siphoviridae sp. ctpQM7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8Q7|A0A5Q2W8Q7_9CAUD Cytosine-specific methyltransferase OS=Siphoviridae sp. ctpQM7 OX=2656722 PE=3 SV=1
MM1 pKa = 7.48AFSRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.01GPRR10 pKa = 11.84DD11 pKa = 3.26VRR13 pKa = 11.84VFPSAGTIAKK23 pKa = 9.89NDD25 pKa = 3.27LVQFDD30 pKa = 4.27EE31 pKa = 4.41NGEE34 pKa = 4.42VVVAATNKK42 pKa = 10.33DD43 pKa = 3.05IVGIALEE50 pKa = 4.46AATSSTNVHH59 pKa = 5.83VDD61 pKa = 2.88VLYY64 pKa = 10.34PGHH67 pKa = 6.39EE68 pKa = 4.43VYY70 pKa = 11.28ADD72 pKa = 3.19ISTGSMAADD81 pKa = 4.37EE82 pKa = 4.7IGEE85 pKa = 4.14EE86 pKa = 4.77ADD88 pKa = 5.38LEE90 pKa = 4.51DD91 pKa = 4.74EE92 pKa = 4.46NSLTLTEE99 pKa = 4.71SNNDD103 pKa = 3.76FIINGWDD110 pKa = 3.45GVSTGKK116 pKa = 10.74CYY118 pKa = 10.54GHH120 pKa = 7.14LRR122 pKa = 11.84FLAFGSSAAPKK133 pKa = 8.84TT134 pKa = 3.83

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W956|A0A5Q2W956_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctpQM7 OX=2656722 PE=4 SV=1
MM1 pKa = 7.52SPLKK5 pKa = 10.34RR6 pKa = 11.84GSSQAVISANIRR18 pKa = 11.84EE19 pKa = 4.58LVRR22 pKa = 11.84SGRR25 pKa = 11.84PQRR28 pKa = 11.84QAIAIAHH35 pKa = 6.54RR36 pKa = 11.84SAGLSRR42 pKa = 11.84KK43 pKa = 8.42RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.34NKK48 pKa = 9.48RR49 pKa = 11.84RR50 pKa = 11.84AKK52 pKa = 10.33

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13081

29

953

186.9

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.791 ± 0.388

1.506 ± 0.228

5.045 ± 0.282

6.169 ± 0.505

4.159 ± 0.235

7.859 ± 0.306

2.248 ± 0.211

5.397 ± 0.202

5.527 ± 0.362

8.501 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.147

3.654 ± 0.238

4.709 ± 0.245

3.746 ± 0.265

7.293 ± 0.325

6.995 ± 0.318

5.886 ± 0.469

6.047 ± 0.301

1.414 ± 0.132

2.722 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski