Amino acid dipepetide frequency for Siphoviridae sp. ctpQM7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.951AlaAla: 7.951 ± 0.894
1.376AlaCys: 1.376 ± 0.352
4.511AlaAsp: 4.511 ± 0.584
5.199AlaGlu: 5.199 ± 0.813
2.982AlaPhe: 2.982 ± 0.433
7.492AlaGly: 7.492 ± 0.914
1.3AlaHis: 1.3 ± 0.321
5.657AlaIle: 5.657 ± 0.634
4.893AlaLys: 4.893 ± 0.655
8.028AlaLeu: 8.028 ± 0.872
2.523AlaMet: 2.523 ± 0.388
3.823AlaAsn: 3.823 ± 0.366
3.517AlaPro: 3.517 ± 0.502
2.982AlaGln: 2.982 ± 0.495
6.575AlaArg: 6.575 ± 0.924
5.275AlaSer: 5.275 ± 0.628
4.74AlaThr: 4.74 ± 0.689
5.122AlaVal: 5.122 ± 0.683
0.994AlaTrp: 0.994 ± 0.312
2.982AlaTyr: 2.982 ± 0.412
0.0AlaXaa: 0.0 ± 0.0
Cys
1.758CysAla: 1.758 ± 0.447
0.153CysCys: 0.153 ± 0.096
0.994CysAsp: 0.994 ± 0.331
0.765CysGlu: 0.765 ± 0.237
0.994CysPhe: 0.994 ± 0.251
1.147CysGly: 1.147 ± 0.303
0.382CysHis: 0.382 ± 0.155
0.765CysIle: 0.765 ± 0.249
0.459CysLys: 0.459 ± 0.226
1.147CysLeu: 1.147 ± 0.374
0.229CysMet: 0.229 ± 0.133
0.306CysAsn: 0.306 ± 0.158
0.765CysPro: 0.765 ± 0.275
0.994CysGln: 0.994 ± 0.367
0.994CysArg: 0.994 ± 0.293
0.765CysSer: 0.765 ± 0.274
0.765CysThr: 0.765 ± 0.239
1.3CysVal: 1.3 ± 0.334
0.076CysTrp: 0.076 ± 0.067
0.306CysTyr: 0.306 ± 0.164
0.0CysXaa: 0.0 ± 0.0
Asp
5.275AspAla: 5.275 ± 0.699
0.765AspCys: 0.765 ± 0.266
2.982AspAsp: 2.982 ± 0.55
2.905AspGlu: 2.905 ± 0.612
3.058AspPhe: 3.058 ± 0.437
4.205AspGly: 4.205 ± 0.529
1.07AspHis: 1.07 ± 0.331
3.364AspIle: 3.364 ± 0.599
1.835AspLys: 1.835 ± 0.313
3.899AspLeu: 3.899 ± 0.535
0.765AspMet: 0.765 ± 0.264
1.835AspAsn: 1.835 ± 0.408
1.988AspPro: 1.988 ± 0.391
1.376AspGln: 1.376 ± 0.246
2.905AspArg: 2.905 ± 0.401
2.905AspSer: 2.905 ± 0.436
3.058AspThr: 3.058 ± 0.387
4.281AspVal: 4.281 ± 0.415
0.765AspTrp: 0.765 ± 0.239
1.223AspTyr: 1.223 ± 0.418
0.0AspXaa: 0.0 ± 0.0
Glu
4.969GluAla: 4.969 ± 0.694
0.917GluCys: 0.917 ± 0.273
3.823GluAsp: 3.823 ± 0.369
5.887GluGlu: 5.887 ± 0.786
1.223GluPhe: 1.223 ± 0.264
4.205GluGly: 4.205 ± 0.4
1.606GluHis: 1.606 ± 0.343
3.211GluIle: 3.211 ± 0.422
5.428GluLys: 5.428 ± 0.626
3.976GluLeu: 3.976 ± 0.505
1.529GluMet: 1.529 ± 0.264
1.988GluAsn: 1.988 ± 0.377
1.911GluPro: 1.911 ± 0.514
2.829GluGln: 2.829 ± 0.419
5.122GluArg: 5.122 ± 0.75
3.67GluSer: 3.67 ± 0.441
3.823GluThr: 3.823 ± 0.453
2.523GluVal: 2.523 ± 0.393
0.994GluTrp: 0.994 ± 0.293
2.064GluTyr: 2.064 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
2.905PheAla: 2.905 ± 0.356
1.3PheCys: 1.3 ± 0.346
2.523PheAsp: 2.523 ± 0.449
1.3PheGlu: 1.3 ± 0.32
2.905PhePhe: 2.905 ± 0.514
2.523PheGly: 2.523 ± 0.48
1.07PheHis: 1.07 ± 0.294
1.758PheIle: 1.758 ± 0.444
1.07PheLys: 1.07 ± 0.271
5.122PheLeu: 5.122 ± 0.706
0.459PheMet: 0.459 ± 0.184
0.765PheAsn: 0.765 ± 0.219
3.211PhePro: 3.211 ± 0.547
1.606PheGln: 1.606 ± 0.361
3.976PheArg: 3.976 ± 0.725
3.517PheSer: 3.517 ± 0.48
1.911PheThr: 1.911 ± 0.411
2.37PheVal: 2.37 ± 0.537
0.382PheTrp: 0.382 ± 0.146
0.917PheTyr: 0.917 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
6.957GlyAla: 6.957 ± 1.146
0.917GlyCys: 0.917 ± 0.244
4.128GlyAsp: 4.128 ± 0.534
6.346GlyGlu: 6.346 ± 0.957
2.37GlyPhe: 2.37 ± 0.32
8.18GlyGly: 8.18 ± 1.37
1.3GlyHis: 1.3 ± 0.318
5.657GlyIle: 5.657 ± 0.67
5.505GlyLys: 5.505 ± 0.602
6.04GlyLeu: 6.04 ± 0.616
2.064GlyMet: 2.064 ± 0.301
2.905GlyAsn: 2.905 ± 0.336
1.223GlyPro: 1.223 ± 0.244
2.141GlyGln: 2.141 ± 0.359
4.74GlyArg: 4.74 ± 0.621
5.199GlySer: 5.199 ± 0.866
4.817GlyThr: 4.817 ± 0.659
4.434GlyVal: 4.434 ± 0.584
1.529GlyTrp: 1.529 ± 0.333
2.141GlyTyr: 2.141 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.911HisAla: 1.911 ± 0.335
0.306HisCys: 0.306 ± 0.178
0.765HisAsp: 0.765 ± 0.221
1.07HisGlu: 1.07 ± 0.275
0.841HisPhe: 0.841 ± 0.269
1.835HisGly: 1.835 ± 0.391
1.223HisHis: 1.223 ± 0.321
0.917HisIle: 0.917 ± 0.241
1.453HisLys: 1.453 ± 0.361
1.988HisLeu: 1.988 ± 0.357
0.306HisMet: 0.306 ± 0.166
1.147HisAsn: 1.147 ± 0.323
0.994HisPro: 0.994 ± 0.316
0.841HisGln: 0.841 ± 0.328
2.217HisArg: 2.217 ± 0.548
1.606HisSer: 1.606 ± 0.375
0.841HisThr: 0.841 ± 0.259
1.376HisVal: 1.376 ± 0.311
0.382HisTrp: 0.382 ± 0.151
0.459HisTyr: 0.459 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
5.887IleAla: 5.887 ± 0.746
0.765IleCys: 0.765 ± 0.263
3.287IleAsp: 3.287 ± 0.474
4.205IleGlu: 4.205 ± 0.697
1.911IlePhe: 1.911 ± 0.434
4.205IleGly: 4.205 ± 0.455
1.3IleHis: 1.3 ± 0.327
1.911IleIle: 1.911 ± 0.379
1.911IleLys: 1.911 ± 0.464
5.122IleLeu: 5.122 ± 0.737
1.147IleMet: 1.147 ± 0.304
1.988IleAsn: 1.988 ± 0.324
2.905IlePro: 2.905 ± 0.41
1.758IleGln: 1.758 ± 0.31
3.593IleArg: 3.593 ± 0.568
3.364IleSer: 3.364 ± 0.457
2.905IleThr: 2.905 ± 0.399
4.434IleVal: 4.434 ± 0.51
0.459IleTrp: 0.459 ± 0.184
0.917IleTyr: 0.917 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
4.511LysAla: 4.511 ± 0.579
0.688LysCys: 0.688 ± 0.218
2.676LysAsp: 2.676 ± 0.351
3.899LysGlu: 3.899 ± 0.766
1.988LysPhe: 1.988 ± 0.366
3.517LysGly: 3.517 ± 0.701
1.223LysHis: 1.223 ± 0.325
2.982LysIle: 2.982 ± 0.508
4.358LysLys: 4.358 ± 0.578
3.44LysLeu: 3.44 ± 0.585
1.758LysMet: 1.758 ± 0.3
2.294LysAsn: 2.294 ± 0.394
2.294LysPro: 2.294 ± 0.509
1.758LysGln: 1.758 ± 0.367
4.511LysArg: 4.511 ± 0.625
3.67LysSer: 3.67 ± 0.656
4.205LysThr: 4.205 ± 0.468
2.829LysVal: 2.829 ± 0.456
0.535LysTrp: 0.535 ± 0.164
2.141LysTyr: 2.141 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
7.875LeuAla: 7.875 ± 0.801
1.911LeuCys: 1.911 ± 0.376
3.517LeuAsp: 3.517 ± 0.525
3.746LeuGlu: 3.746 ± 0.506
3.67LeuPhe: 3.67 ± 0.592
4.358LeuGly: 4.358 ± 0.527
2.294LeuHis: 2.294 ± 0.534
3.058LeuIle: 3.058 ± 0.451
4.205LeuLys: 4.205 ± 0.529
7.187LeuLeu: 7.187 ± 0.828
1.529LeuMet: 1.529 ± 0.364
3.287LeuAsn: 3.287 ± 0.649
5.046LeuPro: 5.046 ± 0.833
3.823LeuGln: 3.823 ± 0.723
7.951LeuArg: 7.951 ± 0.729
8.028LeuSer: 8.028 ± 1.022
5.046LeuThr: 5.046 ± 0.821
4.358LeuVal: 4.358 ± 0.615
1.606LeuTrp: 1.606 ± 0.383
2.446LeuTyr: 2.446 ± 0.456
0.0LeuXaa: 0.0 ± 0.0
Met
2.446MetAla: 2.446 ± 0.41
0.306MetCys: 0.306 ± 0.144
1.07MetAsp: 1.07 ± 0.432
1.758MetGlu: 1.758 ± 0.328
0.994MetPhe: 0.994 ± 0.294
1.682MetGly: 1.682 ± 0.396
0.229MetHis: 0.229 ± 0.124
1.529MetIle: 1.529 ± 0.289
1.07MetLys: 1.07 ± 0.233
1.3MetLeu: 1.3 ± 0.314
0.382MetMet: 0.382 ± 0.21
0.612MetAsn: 0.612 ± 0.249
1.147MetPro: 1.147 ± 0.306
1.147MetGln: 1.147 ± 0.261
2.294MetArg: 2.294 ± 0.409
1.606MetSer: 1.606 ± 0.338
1.3MetThr: 1.3 ± 0.357
1.223MetVal: 1.223 ± 0.283
0.459MetTrp: 0.459 ± 0.156
0.765MetTyr: 0.765 ± 0.268
0.0MetXaa: 0.0 ± 0.0
Asn
4.511AsnAla: 4.511 ± 0.538
0.306AsnCys: 0.306 ± 0.159
1.606AsnAsp: 1.606 ± 0.361
1.529AsnGlu: 1.529 ± 0.359
1.606AsnPhe: 1.606 ± 0.248
2.905AsnGly: 2.905 ± 0.49
0.688AsnHis: 0.688 ± 0.25
2.599AsnIle: 2.599 ± 0.378
1.529AsnLys: 1.529 ± 0.269
2.446AsnLeu: 2.446 ± 0.279
0.765AsnMet: 0.765 ± 0.242
1.529AsnAsn: 1.529 ± 0.308
1.682AsnPro: 1.682 ± 0.486
1.606AsnGln: 1.606 ± 0.352
1.758AsnArg: 1.758 ± 0.344
2.294AsnSer: 2.294 ± 0.549
2.829AsnThr: 2.829 ± 0.578
3.211AsnVal: 3.211 ± 0.414
0.535AsnTrp: 0.535 ± 0.185
0.612AsnTyr: 0.612 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
3.287ProAla: 3.287 ± 0.469
0.535ProCys: 0.535 ± 0.229
2.141ProAsp: 2.141 ± 0.517
2.829ProGlu: 2.829 ± 0.468
2.217ProPhe: 2.217 ± 0.513
1.988ProGly: 1.988 ± 0.366
1.606ProHis: 1.606 ± 0.355
2.294ProIle: 2.294 ± 0.343
1.606ProLys: 1.606 ± 0.373
4.052ProLeu: 4.052 ± 0.631
1.606ProMet: 1.606 ± 0.282
1.223ProAsn: 1.223 ± 0.335
4.893ProPro: 4.893 ± 1.063
2.446ProGln: 2.446 ± 0.449
3.058ProArg: 3.058 ± 0.633
4.205ProSer: 4.205 ± 0.488
2.37ProThr: 2.37 ± 0.373
2.676ProVal: 2.676 ± 0.466
0.765ProTrp: 0.765 ± 0.246
1.3ProTyr: 1.3 ± 0.338
0.0ProXaa: 0.0 ± 0.0
Gln
2.905GlnAla: 2.905 ± 0.427
0.535GlnCys: 0.535 ± 0.217
1.606GlnAsp: 1.606 ± 0.35
2.599GlnGlu: 2.599 ± 0.614
1.911GlnPhe: 1.911 ± 0.41
3.135GlnGly: 3.135 ± 0.577
0.917GlnHis: 0.917 ± 0.302
1.223GlnIle: 1.223 ± 0.261
3.364GlnLys: 3.364 ± 0.682
2.37GlnLeu: 2.37 ± 0.409
1.223GlnMet: 1.223 ± 0.263
1.758GlnAsn: 1.758 ± 0.355
0.917GlnPro: 0.917 ± 0.243
1.835GlnGln: 1.835 ± 0.415
2.982GlnArg: 2.982 ± 0.463
2.37GlnSer: 2.37 ± 0.594
2.064GlnThr: 2.064 ± 0.373
1.988GlnVal: 1.988 ± 0.333
0.841GlnTrp: 0.841 ± 0.274
0.917GlnTyr: 0.917 ± 0.252
0.0GlnXaa: 0.0 ± 0.0
Arg
4.893ArgAla: 4.893 ± 0.58
0.917ArgCys: 0.917 ± 0.322
3.823ArgAsp: 3.823 ± 0.512
5.046ArgGlu: 5.046 ± 0.535
2.829ArgPhe: 2.829 ± 0.393
6.116ArgGly: 6.116 ± 0.734
1.606ArgHis: 1.606 ± 0.34
5.122ArgIle: 5.122 ± 0.646
5.122ArgLys: 5.122 ± 0.637
7.034ArgLeu: 7.034 ± 0.679
2.294ArgMet: 2.294 ± 0.379
2.905ArgAsn: 2.905 ± 0.512
2.217ArgPro: 2.217 ± 0.371
2.446ArgGln: 2.446 ± 0.404
6.116ArgArg: 6.116 ± 0.751
5.046ArgSer: 5.046 ± 0.556
2.446ArgThr: 2.446 ± 0.431
4.511ArgVal: 4.511 ± 0.596
1.223ArgTrp: 1.223 ± 0.318
1.223ArgTyr: 1.223 ± 0.288
0.0ArgXaa: 0.0 ± 0.0
Ser
5.81SerAla: 5.81 ± 0.607
1.147SerCys: 1.147 ± 0.327
2.905SerAsp: 2.905 ± 0.538
2.829SerGlu: 2.829 ± 0.491
3.44SerPhe: 3.44 ± 0.381
8.18SerGly: 8.18 ± 1.345
1.376SerHis: 1.376 ± 0.369
3.517SerIle: 3.517 ± 0.555
2.905SerLys: 2.905 ± 0.534
7.034SerLeu: 7.034 ± 0.783
1.453SerMet: 1.453 ± 0.339
2.599SerAsn: 2.599 ± 0.396
3.899SerPro: 3.899 ± 0.63
2.064SerGln: 2.064 ± 0.518
3.593SerArg: 3.593 ± 0.495
5.81SerSer: 5.81 ± 0.845
4.358SerThr: 4.358 ± 0.579
4.358SerVal: 4.358 ± 0.563
1.147SerTrp: 1.147 ± 0.279
1.529SerTyr: 1.529 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
5.581ThrAla: 5.581 ± 0.611
0.459ThrCys: 0.459 ± 0.18
2.446ThrAsp: 2.446 ± 0.449
2.676ThrGlu: 2.676 ± 0.347
3.287ThrPhe: 3.287 ± 0.602
5.046ThrGly: 5.046 ± 0.829
1.147ThrHis: 1.147 ± 0.285
2.676ThrIle: 2.676 ± 0.4
2.905ThrLys: 2.905 ± 0.486
5.581ThrLeu: 5.581 ± 0.719
1.147ThrMet: 1.147 ± 0.32
2.217ThrAsn: 2.217 ± 0.479
3.364ThrPro: 3.364 ± 0.504
2.37ThrGln: 2.37 ± 0.43
3.287ThrArg: 3.287 ± 0.465
3.976ThrSer: 3.976 ± 0.717
4.817ThrThr: 4.817 ± 1.143
3.899ThrVal: 3.899 ± 0.715
0.306ThrTrp: 0.306 ± 0.154
1.682ThrTyr: 1.682 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
4.74ValAla: 4.74 ± 0.512
1.07ValCys: 1.07 ± 0.243
3.135ValAsp: 3.135 ± 0.359
4.281ValGlu: 4.281 ± 0.564
1.529ValPhe: 1.529 ± 0.411
4.128ValGly: 4.128 ± 0.548
0.841ValHis: 0.841 ± 0.225
3.058ValIle: 3.058 ± 0.432
2.829ValLys: 2.829 ± 0.488
5.81ValLeu: 5.81 ± 0.598
1.223ValMet: 1.223 ± 0.365
2.064ValAsn: 2.064 ± 0.391
4.128ValPro: 4.128 ± 0.585
1.606ValGln: 1.606 ± 0.438
4.511ValArg: 4.511 ± 0.558
4.052ValSer: 4.052 ± 0.493
4.893ValThr: 4.893 ± 0.792
3.593ValVal: 3.593 ± 0.634
0.459ValTrp: 0.459 ± 0.182
2.523ValTyr: 2.523 ± 0.511
0.0ValXaa: 0.0 ± 0.0
Trp
1.147TrpAla: 1.147 ± 0.303
0.076TrpCys: 0.076 ± 0.082
0.153TrpAsp: 0.153 ± 0.103
1.376TrpGlu: 1.376 ± 0.32
0.841TrpPhe: 0.841 ± 0.283
1.147TrpGly: 1.147 ± 0.271
0.459TrpHis: 0.459 ± 0.192
1.07TrpIle: 1.07 ± 0.277
1.682TrpLys: 1.682 ± 0.339
1.376TrpLeu: 1.376 ± 0.276
0.459TrpMet: 0.459 ± 0.15
0.535TrpAsn: 0.535 ± 0.201
0.153TrpPro: 0.153 ± 0.112
0.994TrpGln: 0.994 ± 0.32
0.917TrpArg: 0.917 ± 0.209
0.382TrpSer: 0.382 ± 0.169
0.459TrpThr: 0.459 ± 0.214
0.306TrpVal: 0.306 ± 0.161
0.153TrpTrp: 0.153 ± 0.125
0.459TrpTyr: 0.459 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.599TyrAla: 2.599 ± 0.477
0.612TyrCys: 0.612 ± 0.195
2.37TyrAsp: 2.37 ± 0.314
1.453TyrGlu: 1.453 ± 0.291
0.994TyrPhe: 0.994 ± 0.242
2.599TyrGly: 2.599 ± 0.446
0.841TyrHis: 0.841 ± 0.198
1.606TyrIle: 1.606 ± 0.352
1.147TyrLys: 1.147 ± 0.285
2.064TyrLeu: 2.064 ± 0.381
0.382TyrMet: 0.382 ± 0.146
0.765TyrAsn: 0.765 ± 0.223
0.841TyrPro: 0.841 ± 0.243
0.841TyrGln: 0.841 ± 0.261
1.835TyrArg: 1.835 ± 0.333
2.217TyrSer: 2.217 ± 0.366
1.223TyrThr: 1.223 ± 0.336
1.682TyrVal: 1.682 ± 0.276
0.535TyrTrp: 0.535 ± 0.209
0.612TyrTyr: 0.612 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (13081 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski