Rippkaea orientalis (strain PCC 8801) (Cyanothece sp. (strain PCC 8801))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Rippkaea; Rippkaea orientalis

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7K2V5|B7K2V5_RIPO1 Uncharacterized protein OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=PCC8801_3700 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.36NLVRR6 pKa = 11.84FGVSLPVASILVATSISSAQAAGFTPGDD34 pKa = 3.72IFSGTGDD41 pKa = 3.54SFTLTGTTDD50 pKa = 3.29PTDD53 pKa = 3.68PALYY57 pKa = 10.8ALFCDD62 pKa = 5.15GPINGPSVGIASGGPTSQCAKK83 pKa = 9.63VDD85 pKa = 3.61SSSSFNVSGNPANMTGGFAPFLNTDD110 pKa = 3.88PNALVYY116 pKa = 10.92SFGPANTGPTGTNTGSNIVPVFTTDD141 pKa = 2.85NGALFLTISVDD152 pKa = 3.42TASLVISGAGPGNPVNFFYY171 pKa = 10.55TGIAEE176 pKa = 4.19VTGADD181 pKa = 3.81TYY183 pKa = 11.14SAPITFSFTSQNANLVNNTALVNFAPGQDD212 pKa = 2.94GGIYY216 pKa = 8.77DD217 pKa = 3.72TTIGVNTWSVTVLAVEE233 pKa = 4.55SVPEE237 pKa = 4.05PSTLLGLGLLACGGAAGAIKK257 pKa = 10.34RR258 pKa = 11.84RR259 pKa = 11.84LNKK262 pKa = 10.23

Molecular weight: 26.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7JUB7|B7JUB7_RIPO1 Glyco_hydro_63 domain-containing protein OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=PCC8801_0399 PE=3 SV=1
MM1 pKa = 7.58LISVGFSHH9 pKa = 6.97QEE11 pKa = 3.28ILMSQVQAQQEE22 pKa = 4.08WGRR25 pKa = 11.84LGEE28 pKa = 4.13RR29 pKa = 11.84SVGAQADD36 pKa = 3.97RR37 pKa = 11.84DD38 pKa = 4.35TIIVTGSQGTFRR50 pKa = 11.84QLKK53 pKa = 8.99FVVLRR58 pKa = 11.84HH59 pKa = 6.43PIRR62 pKa = 11.84IYY64 pKa = 10.51RR65 pKa = 11.84IVVHH69 pKa = 5.98YY70 pKa = 10.95GNGRR74 pKa = 11.84SEE76 pKa = 4.35TIEE79 pKa = 3.76TRR81 pKa = 11.84EE82 pKa = 4.16LIRR85 pKa = 11.84AGSEE89 pKa = 3.69SRR91 pKa = 11.84VINLRR96 pKa = 11.84GGDD99 pKa = 3.61RR100 pKa = 11.84VIKK103 pKa = 10.58KK104 pKa = 9.6IDD106 pKa = 3.23FWYY109 pKa = 7.16EE110 pKa = 3.84TKK112 pKa = 10.85SLGKK116 pKa = 9.05NRR118 pKa = 11.84AIIRR122 pKa = 11.84VYY124 pKa = 10.7GRR126 pKa = 11.84SS127 pKa = 3.22

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4335

0

4335

1335749

29

3086

308.1

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.895 ± 0.04

1.024 ± 0.011

4.884 ± 0.024

6.53 ± 0.04

3.992 ± 0.026

6.462 ± 0.041

1.877 ± 0.018

7.398 ± 0.029

5.283 ± 0.035

11.336 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.877 ± 0.017

4.599 ± 0.036

4.745 ± 0.031

5.565 ± 0.037

4.722 ± 0.027

6.346 ± 0.028

5.677 ± 0.032

6.087 ± 0.03

1.445 ± 0.019

3.254 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski