Enterorhabdus caecimuris B7
Average proteome isoelectric point is 5.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2435 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9KVX0|R9KVX0_9ACTN YggT family protein OS=Enterorhabdus caecimuris B7 OX=1235794 GN=C811_00935 PE=3 SV=1
MM1 pKa = 7.58 RR2 pKa = 11.84 MKK4 pKa = 10.61 PILAMLLGAALLPISACSGYY24 pKa = 10.4 DD25 pKa = 3.07 IPEE28 pKa = 4.3 EE29 pKa = 4.26 VPAPAQSDD37 pKa = 3.27 ASNPFEE43 pKa = 4.37 EE44 pKa = 4.79 AQDD47 pKa = 4.13 DD48 pKa = 4.16 ASLMAMLSHH57 pKa = 6.84 EE58 pKa = 4.45 AANPPLSDD66 pKa = 3.23 SGEE69 pKa = 4.09 RR70 pKa = 11.84 EE71 pKa = 3.87 PFPYY75 pKa = 10.37 DD76 pKa = 3.05 GGEE79 pKa = 3.62 FRR81 pKa = 11.84 LDD83 pKa = 3.23 YY84 pKa = 10.38 RR85 pKa = 11.84 FSVSGDD91 pKa = 3.24 LDD93 pKa = 3.69 SVGFLLFLDD102 pKa = 5.48 GKK104 pKa = 7.77 PQPYY108 pKa = 10.08 RR109 pKa = 11.84 IDD111 pKa = 3.89 GQSSDD116 pKa = 3.73 YY117 pKa = 11.21 AFCHH121 pKa = 5.93 TFDD124 pKa = 3.81 AAEE127 pKa = 4.4 DD128 pKa = 3.51 QDD130 pKa = 3.62 IAFLFEE136 pKa = 4.2 PVAGHH141 pKa = 7.09 SGDD144 pKa = 3.79 TLNLTIVSITNPDD157 pKa = 3.6 FQPDD161 pKa = 3.68 MEE163 pKa = 4.65 STSSYY168 pKa = 10.01 GWYY171 pKa = 10.3 HH172 pKa = 6.12 NALSASVEE180 pKa = 4.0 MRR182 pKa = 11.84 FNADD186 pKa = 3.66 PPSSAATAPNPVEE199 pKa = 4.73 AFSSCSVHH207 pKa = 5.54 EE208 pKa = 4.43 EE209 pKa = 4.25 KK210 pKa = 10.0 VTSRR214 pKa = 11.84 YY215 pKa = 10.05 LEE217 pKa = 4.05 NDD219 pKa = 3.53 LAQAGWPDD227 pKa = 3.06 ITMDD231 pKa = 3.77 TLDD234 pKa = 3.98 DD235 pKa = 4.74 GIYY238 pKa = 9.07 YY239 pKa = 9.56 TLTLDD244 pKa = 4.0 GDD246 pKa = 4.44 TVFDD250 pKa = 4.29 NLPLTEE256 pKa = 4.07 PVAVRR261 pKa = 11.84 FTLCGTPGARR271 pKa = 11.84 YY272 pKa = 9.78 NISFFLDD279 pKa = 3.67 HH280 pKa = 6.82 QPMAVNGEE288 pKa = 3.91 ASSAVEE294 pKa = 3.74 LSKK297 pKa = 11.47 GGVAVVEE304 pKa = 4.4 GVIDD308 pKa = 4.01 PSLLGKK314 pKa = 10.62 LSTFYY319 pKa = 10.87 CVAVPADD326 pKa = 3.52 NAGAPFFKK334 pKa = 10.37 TNSILLFQEE343 pKa = 4.22 AA344 pKa = 4.03
Molecular weight: 37.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.706
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.24
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.037
Patrickios 1.316
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.931
Protein with the highest isoelectric point:
>tr|R9LDB6|R9LDB6_9ACTN Dimethylsulfoxide reductase chain B OS=Enterorhabdus caecimuris B7 OX=1235794 GN=C811_00036 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.25 AAGGRR7 pKa = 11.84 GGRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 TPYY15 pKa = 8.01 NTPMRR20 pKa = 11.84 IFLYY24 pKa = 10.41 GISALSWWLSAPATPGEE41 pKa = 4.26 GNRR44 pKa = 11.84 VGQNALRR51 pKa = 11.84 NCKK54 pKa = 9.22 PDD56 pKa = 3.34 AAAIQYY62 pKa = 8.16 LAHH65 pKa = 7.2 CCPQIPRR72 pKa = 11.84 PYY74 pKa = 9.35 HH75 pKa = 4.31 VTVSVRR81 pKa = 11.84 MKK83 pKa = 10.12 RR84 pKa = 11.84 CPDD87 pKa = 2.72 GVAPHH92 pKa = 7.15 RR93 pKa = 11.84 SQFKK97 pKa = 9.78 LVGRR101 pKa = 11.84 PFCRR105 pKa = 11.84 VASGIYY111 pKa = 9.83 APCPEE116 pKa = 4.34 LCFVQVANNLDD127 pKa = 3.37 LHH129 pKa = 6.26 EE130 pKa = 4.35 LVKK133 pKa = 10.96 VGNALCGTFFIDD145 pKa = 3.68 PKK147 pKa = 10.86 ARR149 pKa = 11.84 NGLGKK154 pKa = 10.04 RR155 pKa = 11.84 RR156 pKa = 11.84 PLTSVRR162 pKa = 11.84 RR163 pKa = 11.84 IGAFIEE169 pKa = 4.3 RR170 pKa = 11.84 NPGILGAKK178 pKa = 7.85 PARR181 pKa = 11.84 RR182 pKa = 11.84 ALGLMVDD189 pKa = 4.94 GAASPPEE196 pKa = 4.25 VFLAMALGLPYY207 pKa = 10.56 RR208 pKa = 11.84 FGGYY212 pKa = 7.66 QLPGIAANRR221 pKa = 11.84 RR222 pKa = 11.84 IKK224 pKa = 10.46 PSSKK228 pKa = 10.12 ARR230 pKa = 11.84 AIAHH234 pKa = 7.08 RR235 pKa = 11.84 STLVPDD241 pKa = 3.95 ILCEE245 pKa = 4.04 SSRR248 pKa = 11.84 LDD250 pKa = 3.75 IEE252 pKa = 4.32 YY253 pKa = 10.72 DD254 pKa = 3.61 SNTEE258 pKa = 3.79 HH259 pKa = 6.57 ATAAQLTRR267 pKa = 11.84 DD268 pKa = 3.63 AQKK271 pKa = 11.04 RR272 pKa = 11.84 LALEE276 pKa = 3.89 ADD278 pKa = 4.16 GYY280 pKa = 11.48 KK281 pKa = 10.53 VITVTARR288 pKa = 11.84 QIGSQGEE295 pKa = 3.94 MRR297 pKa = 11.84 KK298 pKa = 9.12 IAEE301 pKa = 3.75 QSRR304 pKa = 11.84 RR305 pKa = 11.84 RR306 pKa = 11.84 MGTRR310 pKa = 11.84 LRR312 pKa = 11.84 PQSAAFRR319 pKa = 11.84 QQQRR323 pKa = 11.84 ALFRR327 pKa = 11.84 TGWSLSRR334 pKa = 11.84 YY335 pKa = 8.43 HH336 pKa = 6.55 RR337 pKa = 11.84 RR338 pKa = 11.84 EE339 pKa = 3.83 WLEE342 pKa = 3.82 GAPEE346 pKa = 4.03 PVAPAPLCPTPVDD359 pKa = 3.45 ALTAPGRR366 pKa = 11.84 ALRR369 pKa = 11.84 CPAVPKK375 pKa = 10.3 RR376 pKa = 11.84 WPLSDD381 pKa = 4.09 AGTFSGLKK389 pKa = 8.94 STTLEE394 pKa = 4.2 PNSPEE399 pKa = 3.98 IEE401 pKa = 4.51 GGASPGITPRR411 pKa = 11.84 GNTPEE416 pKa = 3.86 MM417 pKa = 4.55
Molecular weight: 45.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.589
IPC_protein 10.452
Toseland 10.555
ProMoST 10.306
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.818
Grimsley 10.73
Solomon 10.789
Lehninger 10.745
Nozaki 10.584
DTASelect 10.409
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.482
IPC_peptide 10.789
IPC2_peptide 9.692
IPC2.peptide.svr19 8.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2435
0
2435
846340
26
24897
347.6
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.98 ± 0.096
1.518 ± 0.046
6.097 ± 0.116
6.724 ± 0.058
3.642 ± 0.042
8.474 ± 0.048
1.671 ± 0.021
4.649 ± 0.071
3.614 ± 0.055
8.96 ± 0.125
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.049
2.851 ± 0.09
4.582 ± 0.033
2.636 ± 0.026
5.992 ± 0.086
5.488 ± 0.042
5.443 ± 0.117
8.05 ± 0.045
1.174 ± 0.021
2.902 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here