Akkermansia glycaniphila
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2530 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C7PEE3|A0A1C7PEE3_9BACT von willebrand factor type a OS=Akkermansia glycaniphila OX=1679444 GN=PYTT_0121 PE=4 SV=1
MM1 pKa = 7.57 SNNIPDD7 pKa = 3.83 NLLYY11 pKa = 10.98 SRR13 pKa = 11.84 DD14 pKa = 4.03 HH15 pKa = 6.36 EE16 pKa = 4.49 WVDD19 pKa = 3.28 VDD21 pKa = 5.38 GDD23 pKa = 3.55 IATIGISDD31 pKa = 4.01 HH32 pKa = 6.44 AQAEE36 pKa = 4.33 LGDD39 pKa = 4.07 VVFVDD44 pKa = 4.81 LPEE47 pKa = 5.27 PGTSIAANDD56 pKa = 3.99 SIAVVEE62 pKa = 4.45 SVKK65 pKa = 10.63 AASDD69 pKa = 3.79 VYY71 pKa = 11.15 SPVSGEE77 pKa = 3.64 IIEE80 pKa = 4.34 VNEE83 pKa = 3.99 EE84 pKa = 3.98 LGNEE88 pKa = 3.97 PGAVNSDD95 pKa = 3.58 PYY97 pKa = 11.06 GNGWICKK104 pKa = 9.27 IRR106 pKa = 11.84 LQDD109 pKa = 3.54 MSEE112 pKa = 4.14 LDD114 pKa = 3.97 SLLSPADD121 pKa = 3.49 YY122 pKa = 11.47 SEE124 pKa = 4.58 FCSS127 pKa = 3.96
Molecular weight: 13.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.605
IPC_protein 3.579
Toseland 3.376
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.745
Wikipedia 3.528
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.516
Nozaki 3.706
DTASelect 3.91
Thurlkill 3.439
EMBOSS 3.528
Sillero 3.706
Patrickios 0.846
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.685
Protein with the highest isoelectric point:
>tr|A0A1H6M0L2|A0A1H6M0L2_9BACT 6-phosphofructokinase OS=Akkermansia glycaniphila OX=1679444 GN=PYTT_1977 PE=4 SV=1
MM1 pKa = 7.41 LLVEE5 pKa = 4.53 KK6 pKa = 9.23 TAKK9 pKa = 9.92 RR10 pKa = 11.84 LKK12 pKa = 10.72 SGGLPPVRR20 pKa = 11.84 KK21 pKa = 9.87 NGLLRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 GFGKK32 pKa = 10.49 APARR36 pKa = 11.84 LLPPAIRR43 pKa = 11.84 MGKK46 pKa = 9.97 NIGHH50 pKa = 6.3 GAKK53 pKa = 10.28 KK54 pKa = 10.22 LFEE57 pKa = 4.24 TGRR60 pKa = 11.84 PPKK63 pKa = 10.34 RR64 pKa = 11.84 RR65 pKa = 11.84 TKK67 pKa = 10.22 PILRR71 pKa = 11.84 HH72 pKa = 5.43 RR73 pKa = 11.84 RR74 pKa = 11.84 SKK76 pKa = 10.56 IRR78 pKa = 11.84 TKK80 pKa = 8.6 QRR82 pKa = 11.84 KK83 pKa = 7.33 IAGRR87 pKa = 11.84 QIQSRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 IARR97 pKa = 11.84 RR98 pKa = 11.84 LLARR102 pKa = 11.84 HH103 pKa = 5.76 RR104 pKa = 11.84 QPPQLLPRR112 pKa = 11.84 RR113 pKa = 11.84 AQRR116 pKa = 11.84 THH118 pKa = 6.53 LRR120 pKa = 11.84 HH121 pKa = 6.19 KK122 pKa = 10.11 PPEE125 pKa = 3.86 PRR127 pKa = 11.84 LIRR130 pKa = 11.84 RR131 pKa = 11.84 SGTRR135 pKa = 11.84 LSPPPGSPRR144 pKa = 11.84 HH145 pKa = 6.08 IPHH148 pKa = 6.32 EE149 pKa = 3.74 QRR151 pKa = 11.84 IRR153 pKa = 11.84 IRR155 pKa = 11.84 LRR157 pKa = 11.84 LRR159 pKa = 11.84 MEE161 pKa = 3.92 RR162 pKa = 11.84 QTTVNRR168 pKa = 11.84 RR169 pKa = 11.84 PAPLILPRR177 pKa = 11.84 PRR179 pKa = 11.84 PLRR182 pKa = 11.84 LRR184 pKa = 11.84 LHH186 pKa = 6.94 PLPEE190 pKa = 4.54 ILNLQIRR197 pKa = 11.84 PLLHH201 pKa = 6.97 RR202 pKa = 11.84 VQTDD206 pKa = 3.22 KK207 pKa = 11.51 AHH209 pKa = 6.8 PLAFTEE215 pKa = 4.63 TAGNTSFTHH224 pKa = 7.4 LIDD227 pKa = 3.62 LQRR230 pKa = 11.84 IQMRR234 pKa = 11.84 AQHH237 pKa = 5.69 SRR239 pKa = 11.84 AGIIAAVIVRR249 pKa = 11.84 HH250 pKa = 5.98 RR251 pKa = 11.84 KK252 pKa = 7.43 QAAVKK257 pKa = 9.92 HH258 pKa = 5.46 LRR260 pKa = 11.84 LPITEE265 pKa = 3.94 RR266 pKa = 11.84 SKK268 pKa = 9.96 TIRR271 pKa = 11.84 SQNRR275 pKa = 11.84 SSS277 pKa = 3.07
Molecular weight: 32.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.395
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.252
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.959
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2530
0
2530
925710
32
4417
365.9
40.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.942 ± 0.062
1.442 ± 0.022
5.493 ± 0.031
5.989 ± 0.063
3.646 ± 0.032
7.817 ± 0.088
2.201 ± 0.028
5.502 ± 0.045
4.655 ± 0.053
9.615 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.641 ± 0.024
3.615 ± 0.058
5.096 ± 0.054
3.549 ± 0.032
5.991 ± 0.067
6.126 ± 0.053
5.747 ± 0.084
6.31 ± 0.057
1.512 ± 0.019
3.113 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here