Arthrobacter phage Molivia
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286S2C6|A0A286S2C6_9CAUD DnaQ-like exonuclease OS=Arthrobacter phage Molivia OX=2015839 GN=50 PE=4 SV=1
MM1 pKa = 7.41 TEE3 pKa = 4.14 SNVEE7 pKa = 3.83 PVEE10 pKa = 4.19 TEE12 pKa = 4.04 EE13 pKa = 4.33 EE14 pKa = 4.46 VVEE17 pKa = 4.3 PEE19 pKa = 4.42 LKK21 pKa = 10.52 EE22 pKa = 3.62 VDD24 pKa = 4.05 NIVSQIRR31 pKa = 11.84 YY32 pKa = 9.54 APDD35 pKa = 3.87 PCSTCGG41 pKa = 3.28
Molecular weight: 4.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.848
IPC2_protein 3.897
IPC_protein 3.668
Toseland 3.541
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.516
Rodwell 3.528
Grimsley 3.478
Solomon 3.579
Lehninger 3.528
Nozaki 3.783
DTASelect 3.795
Thurlkill 3.592
EMBOSS 3.541
Sillero 3.783
Patrickios 1.799
IPC_peptide 3.579
IPC2_peptide 3.757
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A286N4C6|A0A286N4C6_9CAUD Uncharacterized protein OS=Arthrobacter phage Molivia OX=2015839 GN=2 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.61 NSGPRR7 pKa = 11.84 VKK9 pKa = 10.24 IEE11 pKa = 3.71 IAHH14 pKa = 4.99 KK15 pKa = 9.41 QGRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LTTFLRR26 pKa = 11.84 ADD28 pKa = 3.76 NATTWDD34 pKa = 4.5 DD35 pKa = 3.29 IQRR38 pKa = 11.84 AAEE41 pKa = 3.91 WQANLVGVPGGFQQHH56 pKa = 5.33 SRR58 pKa = 11.84 EE59 pKa = 4.01 IRR61 pKa = 11.84 PVLAHH66 pKa = 6.32 KK67 pKa = 10.33 LYY69 pKa = 10.95 YY70 pKa = 10.26 RR71 pKa = 11.84 GATQTITEE79 pKa = 4.29 MPP81 pKa = 3.84
Molecular weight: 9.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.745
ProMoST 10.467
Dawson 10.833
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 10.979
Lehninger 10.95
Nozaki 10.716
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.76
Patrickios 10.804
IPC_peptide 10.994
IPC2_peptide 9.355
IPC2.peptide.svr19 8.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
88
0
88
17012
33
1580
193.3
21.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.893 ± 0.592
0.841 ± 0.145
6.207 ± 0.188
7.16 ± 0.395
3.327 ± 0.129
7.312 ± 0.329
1.899 ± 0.177
4.996 ± 0.132
5.49 ± 0.282
7.936 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.141
4.409 ± 0.183
4.191 ± 0.245
3.809 ± 0.229
5.373 ± 0.215
5.655 ± 0.235
6.642 ± 0.322
7.395 ± 0.258
1.799 ± 0.165
3.157 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here