Amino acid dipepetide frequency for Arthrobacter phage Molivia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.757AlaAla: 11.757 ± 1.85
0.118AlaCys: 0.118 ± 0.074
6.408AlaAsp: 6.408 ± 0.732
6.055AlaGlu: 6.055 ± 0.642
2.998AlaPhe: 2.998 ± 0.448
7.289AlaGly: 7.289 ± 0.81
2.351AlaHis: 2.351 ± 0.432
5.114AlaIle: 5.114 ± 0.697
6.525AlaLys: 6.525 ± 0.768
7.348AlaLeu: 7.348 ± 0.635
2.939AlaMet: 2.939 ± 0.377
4.056AlaAsn: 4.056 ± 0.711
3.88AlaPro: 3.88 ± 0.616
4.056AlaGln: 4.056 ± 0.69
5.232AlaArg: 5.232 ± 0.543
5.879AlaSer: 5.879 ± 0.671
6.114AlaThr: 6.114 ± 0.791
7.289AlaVal: 7.289 ± 0.76
1.646AlaTrp: 1.646 ± 0.342
1.881AlaTyr: 1.881 ± 0.384
0.0AlaXaa: 0.0 ± 0.0
Cys
0.999CysAla: 0.999 ± 0.253
0.118CysCys: 0.118 ± 0.076
0.529CysAsp: 0.529 ± 0.167
0.588CysGlu: 0.588 ± 0.193
0.059CysPhe: 0.059 ± 0.057
1.176CysGly: 1.176 ± 0.277
0.176CysHis: 0.176 ± 0.103
0.353CysIle: 0.353 ± 0.142
0.47CysLys: 0.47 ± 0.173
0.411CysLeu: 0.411 ± 0.168
0.059CysMet: 0.059 ± 0.06
0.353CysAsn: 0.353 ± 0.149
0.588CysPro: 0.588 ± 0.184
0.353CysGln: 0.353 ± 0.148
0.588CysArg: 0.588 ± 0.187
0.353CysSer: 0.353 ± 0.145
0.235CysThr: 0.235 ± 0.135
0.47CysVal: 0.47 ± 0.196
0.176CysTrp: 0.176 ± 0.105
0.353CysTyr: 0.353 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
5.702AspAla: 5.702 ± 0.604
0.529AspCys: 0.529 ± 0.192
3.645AspAsp: 3.645 ± 0.458
4.526AspGlu: 4.526 ± 0.619
2.704AspPhe: 2.704 ± 0.404
5.291AspGly: 5.291 ± 0.514
0.999AspHis: 0.999 ± 0.261
3.351AspIle: 3.351 ± 0.442
3.057AspLys: 3.057 ± 0.449
4.879AspLeu: 4.879 ± 0.569
1.94AspMet: 1.94 ± 0.335
2.939AspAsn: 2.939 ± 0.373
2.88AspPro: 2.88 ± 0.464
1.881AspGln: 1.881 ± 0.313
3.292AspArg: 3.292 ± 0.418
3.233AspSer: 3.233 ± 0.488
3.527AspThr: 3.527 ± 0.413
3.703AspVal: 3.703 ± 0.481
1.411AspTrp: 1.411 ± 0.358
2.528AspTyr: 2.528 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
8.289GluAla: 8.289 ± 0.705
0.529GluCys: 0.529 ± 0.193
3.586GluAsp: 3.586 ± 0.538
5.291GluGlu: 5.291 ± 0.751
3.057GluPhe: 3.057 ± 0.475
4.35GluGly: 4.35 ± 0.492
1.646GluHis: 1.646 ± 0.421
3.939GluIle: 3.939 ± 0.406
2.998GluLys: 2.998 ± 0.458
6.525GluLeu: 6.525 ± 0.66
2.057GluMet: 2.057 ± 0.36
2.704GluAsn: 2.704 ± 0.39
2.822GluPro: 2.822 ± 0.386
2.88GluGln: 2.88 ± 0.296
3.468GluArg: 3.468 ± 0.408
3.762GluSer: 3.762 ± 0.44
4.468GluThr: 4.468 ± 0.581
5.643GluVal: 5.643 ± 0.472
1.47GluTrp: 1.47 ± 0.332
2.116GluTyr: 2.116 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
2.88PheAla: 2.88 ± 0.381
0.411PheCys: 0.411 ± 0.149
2.41PheAsp: 2.41 ± 0.373
1.881PheGlu: 1.881 ± 0.287
1.058PhePhe: 1.058 ± 0.232
2.88PheGly: 2.88 ± 0.473
0.823PheHis: 0.823 ± 0.227
1.293PheIle: 1.293 ± 0.315
1.411PheLys: 1.411 ± 0.258
1.999PheLeu: 1.999 ± 0.354
0.823PheMet: 0.823 ± 0.32
1.47PheAsn: 1.47 ± 0.299
0.941PhePro: 0.941 ± 0.203
1.822PheGln: 1.822 ± 0.393
2.057PheArg: 2.057 ± 0.415
2.763PheSer: 2.763 ± 0.4
2.528PheThr: 2.528 ± 0.37
2.822PheVal: 2.822 ± 0.415
0.235PheTrp: 0.235 ± 0.114
0.764PheTyr: 0.764 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
6.878GlyAla: 6.878 ± 1.083
0.47GlyCys: 0.47 ± 0.165
3.762GlyAsp: 3.762 ± 0.455
5.702GlyGlu: 5.702 ± 0.445
2.763GlyPhe: 2.763 ± 0.385
5.114GlyGly: 5.114 ± 0.74
1.822GlyHis: 1.822 ± 0.375
3.174GlyIle: 3.174 ± 0.436
4.585GlyLys: 4.585 ± 0.657
5.585GlyLeu: 5.585 ± 0.516
2.057GlyMet: 2.057 ± 0.369
3.174GlyAsn: 3.174 ± 0.428
2.234GlyPro: 2.234 ± 0.348
2.587GlyGln: 2.587 ± 0.362
3.586GlyArg: 3.586 ± 0.414
4.174GlySer: 4.174 ± 0.459
4.468GlyThr: 4.468 ± 0.457
6.231GlyVal: 6.231 ± 0.702
1.764GlyTrp: 1.764 ± 0.349
2.998GlyTyr: 2.998 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.411HisAla: 1.411 ± 0.28
0.059HisCys: 0.059 ± 0.056
1.47HisAsp: 1.47 ± 0.311
1.293HisGlu: 1.293 ± 0.279
0.411HisPhe: 0.411 ± 0.128
1.293HisGly: 1.293 ± 0.274
0.353HisHis: 0.353 ± 0.127
1.234HisIle: 1.234 ± 0.255
0.941HisLys: 0.941 ± 0.245
1.764HisLeu: 1.764 ± 0.319
0.588HisMet: 0.588 ± 0.188
0.764HisAsn: 0.764 ± 0.214
0.882HisPro: 0.882 ± 0.233
0.588HisGln: 0.588 ± 0.208
1.646HisArg: 1.646 ± 0.352
0.941HisSer: 0.941 ± 0.252
1.176HisThr: 1.176 ± 0.289
1.352HisVal: 1.352 ± 0.285
0.235HisTrp: 0.235 ± 0.106
0.588HisTyr: 0.588 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
5.232IleAla: 5.232 ± 0.54
0.764IleCys: 0.764 ± 0.207
4.174IleAsp: 4.174 ± 0.422
4.174IleGlu: 4.174 ± 0.44
1.176IlePhe: 1.176 ± 0.242
3.174IleGly: 3.174 ± 0.472
0.823IleHis: 0.823 ± 0.224
2.234IleIle: 2.234 ± 0.373
2.645IleLys: 2.645 ± 0.486
2.704IleLeu: 2.704 ± 0.346
1.293IleMet: 1.293 ± 0.324
2.293IleAsn: 2.293 ± 0.301
2.645IlePro: 2.645 ± 0.43
2.175IleGln: 2.175 ± 0.317
2.822IleArg: 2.822 ± 0.389
2.057IleSer: 2.057 ± 0.364
3.586IleThr: 3.586 ± 0.576
3.762IleVal: 3.762 ± 0.419
0.764IleTrp: 0.764 ± 0.199
1.47IleTyr: 1.47 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
6.055LysAla: 6.055 ± 0.571
0.411LysCys: 0.411 ± 0.14
2.763LysAsp: 2.763 ± 0.403
3.703LysGlu: 3.703 ± 0.56
1.176LysPhe: 1.176 ± 0.246
3.174LysGly: 3.174 ± 0.442
1.058LysHis: 1.058 ± 0.264
3.292LysIle: 3.292 ± 0.435
2.998LysLys: 2.998 ± 0.364
5.232LysLeu: 5.232 ± 0.517
1.587LysMet: 1.587 ± 0.29
2.175LysAsn: 2.175 ± 0.417
2.057LysPro: 2.057 ± 0.372
2.822LysGln: 2.822 ± 0.457
3.292LysArg: 3.292 ± 0.45
2.998LysSer: 2.998 ± 0.427
3.057LysThr: 3.057 ± 0.454
4.35LysVal: 4.35 ± 0.554
0.999LysTrp: 0.999 ± 0.262
1.705LysTyr: 1.705 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
8.23LeuAla: 8.23 ± 0.725
0.47LeuCys: 0.47 ± 0.169
5.232LeuAsp: 5.232 ± 0.484
5.643LeuGlu: 5.643 ± 0.809
2.234LeuPhe: 2.234 ± 0.382
6.114LeuGly: 6.114 ± 0.578
1.411LeuHis: 1.411 ± 0.267
4.174LeuIle: 4.174 ± 0.498
3.88LeuLys: 3.88 ± 0.421
5.702LeuLeu: 5.702 ± 0.671
2.234LeuMet: 2.234 ± 0.373
3.703LeuAsn: 3.703 ± 0.348
3.351LeuPro: 3.351 ± 0.413
3.233LeuGln: 3.233 ± 0.525
4.056LeuArg: 4.056 ± 0.473
4.526LeuSer: 4.526 ± 0.567
5.467LeuThr: 5.467 ± 0.674
5.585LeuVal: 5.585 ± 0.535
1.528LeuTrp: 1.528 ± 0.41
2.587LeuTyr: 2.587 ± 0.336
0.0LeuXaa: 0.0 ± 0.0
Met
2.469MetAla: 2.469 ± 0.407
0.235MetCys: 0.235 ± 0.115
0.941MetAsp: 0.941 ± 0.189
1.881MetGlu: 1.881 ± 0.305
0.882MetPhe: 0.882 ± 0.248
1.646MetGly: 1.646 ± 0.325
0.647MetHis: 0.647 ± 0.221
0.882MetIle: 0.882 ± 0.238
1.411MetLys: 1.411 ± 0.261
1.822MetLeu: 1.822 ± 0.299
0.235MetMet: 0.235 ± 0.126
0.999MetAsn: 0.999 ± 0.26
1.293MetPro: 1.293 ± 0.299
0.941MetGln: 0.941 ± 0.263
1.881MetArg: 1.881 ± 0.377
1.764MetSer: 1.764 ± 0.313
2.822MetThr: 2.822 ± 0.389
1.528MetVal: 1.528 ± 0.323
0.411MetTrp: 0.411 ± 0.152
0.411MetTyr: 0.411 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
4.762AsnAla: 4.762 ± 0.594
0.294AsnCys: 0.294 ± 0.128
2.763AsnAsp: 2.763 ± 0.363
3.586AsnGlu: 3.586 ± 0.424
1.411AsnPhe: 1.411 ± 0.269
3.645AsnGly: 3.645 ± 0.464
0.47AsnHis: 0.47 ± 0.181
1.999AsnIle: 1.999 ± 0.476
2.88AsnLys: 2.88 ± 0.466
3.41AsnLeu: 3.41 ± 0.625
1.352AsnMet: 1.352 ± 0.263
1.293AsnAsn: 1.293 ± 0.287
2.88AsnPro: 2.88 ± 0.389
0.999AsnGln: 0.999 ± 0.293
2.528AsnArg: 2.528 ± 0.32
2.822AsnSer: 2.822 ± 0.402
2.057AsnThr: 2.057 ± 0.312
2.763AsnVal: 2.763 ± 0.435
0.705AsnTrp: 0.705 ± 0.21
1.47AsnTyr: 1.47 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
3.292ProAla: 3.292 ± 0.421
0.529ProCys: 0.529 ± 0.18
3.116ProAsp: 3.116 ± 0.569
3.821ProGlu: 3.821 ± 0.457
1.705ProPhe: 1.705 ± 0.26
2.469ProGly: 2.469 ± 0.388
0.705ProHis: 0.705 ± 0.177
1.47ProIle: 1.47 ± 0.267
2.704ProLys: 2.704 ± 0.501
2.998ProLeu: 2.998 ± 0.482
0.647ProMet: 0.647 ± 0.193
2.116ProAsn: 2.116 ± 0.393
1.528ProPro: 1.528 ± 0.28
1.176ProGln: 1.176 ± 0.249
1.94ProArg: 1.94 ± 0.275
2.587ProSer: 2.587 ± 0.445
3.41ProThr: 3.41 ± 0.478
3.351ProVal: 3.351 ± 0.551
0.705ProTrp: 0.705 ± 0.21
1.646ProTyr: 1.646 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
4.291GlnAla: 4.291 ± 0.643
0.411GlnCys: 0.411 ± 0.169
1.999GlnAsp: 1.999 ± 0.327
2.763GlnGlu: 2.763 ± 0.403
1.705GlnPhe: 1.705 ± 0.262
2.293GlnGly: 2.293 ± 0.503
0.764GlnHis: 0.764 ± 0.224
2.587GlnIle: 2.587 ± 0.415
1.528GlnLys: 1.528 ± 0.319
3.057GlnLeu: 3.057 ± 0.453
1.058GlnMet: 1.058 ± 0.254
1.587GlnAsn: 1.587 ± 0.335
1.352GlnPro: 1.352 ± 0.277
2.293GlnGln: 2.293 ± 0.589
1.999GlnArg: 1.999 ± 0.346
1.764GlnSer: 1.764 ± 0.359
2.41GlnThr: 2.41 ± 0.402
2.234GlnVal: 2.234 ± 0.388
0.823GlnTrp: 0.823 ± 0.212
1.176GlnTyr: 1.176 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
4.526ArgAla: 4.526 ± 0.572
0.588ArgCys: 0.588 ± 0.186
2.939ArgAsp: 2.939 ± 0.395
3.233ArgGlu: 3.233 ± 0.474
1.705ArgPhe: 1.705 ± 0.336
3.821ArgGly: 3.821 ± 0.45
1.176ArgHis: 1.176 ± 0.341
3.174ArgIle: 3.174 ± 0.397
2.88ArgLys: 2.88 ± 0.45
5.056ArgLeu: 5.056 ± 0.521
1.117ArgMet: 1.117 ± 0.231
2.234ArgAsn: 2.234 ± 0.255
1.94ArgPro: 1.94 ± 0.359
2.469ArgGln: 2.469 ± 0.318
2.88ArgArg: 2.88 ± 0.459
2.939ArgSer: 2.939 ± 0.337
3.351ArgThr: 3.351 ± 0.501
4.997ArgVal: 4.997 ± 0.64
0.941ArgTrp: 0.941 ± 0.211
1.764ArgTyr: 1.764 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
5.467SerAla: 5.467 ± 0.709
0.353SerCys: 0.353 ± 0.146
3.703SerAsp: 3.703 ± 0.462
3.351SerGlu: 3.351 ± 0.448
1.94SerPhe: 1.94 ± 0.41
4.762SerGly: 4.762 ± 0.581
0.588SerHis: 0.588 ± 0.186
3.116SerIle: 3.116 ± 0.333
3.41SerLys: 3.41 ± 0.503
5.643SerLeu: 5.643 ± 0.62
1.352SerMet: 1.352 ± 0.278
3.057SerAsn: 3.057 ± 0.427
1.411SerPro: 1.411 ± 0.296
1.764SerGln: 1.764 ± 0.317
1.999SerArg: 1.999 ± 0.395
3.292SerSer: 3.292 ± 0.373
4.056SerThr: 4.056 ± 0.555
4.762SerVal: 4.762 ± 0.732
0.705SerTrp: 0.705 ± 0.215
1.822SerTyr: 1.822 ± 0.382
0.0SerXaa: 0.0 ± 0.0
Thr
7.054ThrAla: 7.054 ± 0.704
0.411ThrCys: 0.411 ± 0.159
3.527ThrAsp: 3.527 ± 0.387
5.173ThrGlu: 5.173 ± 0.583
2.528ThrPhe: 2.528 ± 0.363
5.349ThrGly: 5.349 ± 0.69
1.058ThrHis: 1.058 ± 0.299
3.939ThrIle: 3.939 ± 0.474
3.762ThrLys: 3.762 ± 0.47
6.231ThrLeu: 6.231 ± 0.772
1.176ThrMet: 1.176 ± 0.285
2.88ThrAsn: 2.88 ± 0.521
2.939ThrPro: 2.939 ± 0.477
1.822ThrGln: 1.822 ± 0.329
3.41ThrArg: 3.41 ± 0.458
3.057ThrSer: 3.057 ± 0.35
4.468ThrThr: 4.468 ± 0.723
4.82ThrVal: 4.82 ± 0.653
1.058ThrTrp: 1.058 ± 0.285
1.764ThrTyr: 1.764 ± 0.292
0.0ThrXaa: 0.0 ± 0.0
Val
6.055ValAla: 6.055 ± 0.745
0.999ValCys: 0.999 ± 0.269
5.349ValAsp: 5.349 ± 0.428
5.408ValGlu: 5.408 ± 0.571
2.116ValPhe: 2.116 ± 0.374
5.526ValGly: 5.526 ± 0.654
1.176ValHis: 1.176 ± 0.248
2.822ValIle: 2.822 ± 0.443
4.233ValLys: 4.233 ± 0.529
5.173ValLeu: 5.173 ± 0.654
1.47ValMet: 1.47 ± 0.283
3.939ValAsn: 3.939 ± 0.454
3.88ValPro: 3.88 ± 0.494
2.822ValGln: 2.822 ± 0.369
3.821ValArg: 3.821 ± 0.518
5.232ValSer: 5.232 ± 0.589
5.232ValThr: 5.232 ± 0.585
4.82ValVal: 4.82 ± 0.635
1.352ValTrp: 1.352 ± 0.276
2.528ValTyr: 2.528 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
1.587TrpAla: 1.587 ± 0.295
0.235TrpCys: 0.235 ± 0.119
1.528TrpAsp: 1.528 ± 0.312
1.352TrpGlu: 1.352 ± 0.281
0.647TrpPhe: 0.647 ± 0.205
1.117TrpGly: 1.117 ± 0.237
0.588TrpHis: 0.588 ± 0.19
0.47TrpIle: 0.47 ± 0.145
0.823TrpLys: 0.823 ± 0.244
1.646TrpLeu: 1.646 ± 0.392
0.235TrpMet: 0.235 ± 0.117
0.647TrpAsn: 0.647 ± 0.209
1.058TrpPro: 1.058 ± 0.278
0.647TrpGln: 0.647 ± 0.279
1.234TrpArg: 1.234 ± 0.276
0.823TrpSer: 0.823 ± 0.281
0.941TrpThr: 0.941 ± 0.223
1.234TrpVal: 1.234 ± 0.286
0.529TrpTrp: 0.529 ± 0.151
0.647TrpTyr: 0.647 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.999TyrAla: 1.999 ± 0.44
0.47TyrCys: 0.47 ± 0.167
2.234TyrAsp: 2.234 ± 0.282
2.175TyrGlu: 2.175 ± 0.317
0.999TyrPhe: 0.999 ± 0.223
2.645TyrGly: 2.645 ± 0.362
0.353TyrHis: 0.353 ± 0.154
1.352TyrIle: 1.352 ± 0.293
1.764TyrLys: 1.764 ± 0.371
2.175TyrLeu: 2.175 ± 0.421
0.823TyrMet: 0.823 ± 0.24
1.705TyrAsn: 1.705 ± 0.278
1.352TyrPro: 1.352 ± 0.261
0.764TyrGln: 0.764 ± 0.205
1.999TyrArg: 1.999 ± 0.311
1.587TyrSer: 1.587 ± 0.293
3.057TyrThr: 3.057 ± 0.358
2.234TyrVal: 2.234 ± 0.397
0.529TyrTrp: 0.529 ± 0.135
1.352TyrTyr: 1.352 ± 0.283
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (17012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski