Streptomyces sp. Ag109_O5-1
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10263 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4S336|A0A3N4S336_9ACTN Uncharacterized protein OS=Streptomyces sp. Ag109_O5-1 OX=1938851 GN=EDD90_2844 PE=4 SV=1
MM1 pKa = 7.61 AKK3 pKa = 10.23 AFQSRR8 pKa = 11.84 WRR10 pKa = 11.84 QLLGIGRR17 pKa = 11.84 NTARR21 pKa = 11.84 RR22 pKa = 11.84 VAAALLLDD30 pKa = 3.53 PTVPVGNFPTGVAINSAGTLAYY52 pKa = 9.78 VANEE56 pKa = 4.06 GDD58 pKa = 3.98 DD59 pKa = 3.86 SVSVIDD65 pKa = 3.69 TATNAVIATINVGSSPFGVAVTPDD89 pKa = 3.13 GAFVYY94 pKa = 8.04 VTNVASDD101 pKa = 3.8 SVSVIDD107 pKa = 3.7 TATNAVTDD115 pKa = 4.13 TVSVGISPFGIAINAAGTLAYY136 pKa = 9.49 VANVGDD142 pKa = 3.9 NTVSVIDD149 pKa = 3.74 TSTNTVVTDD158 pKa = 3.6 IPVGANPFGVALTPDD173 pKa = 3.19 GAFAYY178 pKa = 7.53 VTNNASGTVSVVDD191 pKa = 3.42 TATNAEE197 pKa = 4.12 IDD199 pKa = 4.05 TIAVGTSPTGVAITSDD215 pKa = 3.3 GVLAYY220 pKa = 8.19 VTNNSDD226 pKa = 3.52 NTVSAIDD233 pKa = 3.36 IATNTVSDD241 pKa = 3.84 TFDD244 pKa = 3.15 VGSAPTSVAFTPDD257 pKa = 3.07 DD258 pKa = 3.48 AFAYY262 pKa = 7.0 VTNNASDD269 pKa = 3.77 TVSVVITATNDD280 pKa = 2.96 VTFDD284 pKa = 3.47 IPVGSGPFGVAITPDD299 pKa = 3.63 GEE301 pKa = 4.21 HH302 pKa = 7.3 AYY304 pKa = 8.65 VTNNASDD311 pKa = 3.97 TVSVTDD317 pKa = 3.51 TVPFPTTTTLTSAPDD332 pKa = 3.55 PSAFGEE338 pKa = 4.27 AKK340 pKa = 10.54 VLTATVTSSTGTPDD354 pKa = 3.14 GTVTFLDD361 pKa = 4.29 GATPLGTVTLAGGVATLTTSSLAVGSHH388 pKa = 6.26 SLTADD393 pKa = 3.2 YY394 pKa = 10.95 AGNVNFSVSTSAADD408 pKa = 3.3 IQTVNAADD416 pKa = 3.69 TATTLTSSPDD426 pKa = 3.23 PSVFGEE432 pKa = 4.14 AKK434 pKa = 10.48 VLTATVTAVAPGSGTPTGSVSFLDD458 pKa = 3.99 GATVIGIGTLDD469 pKa = 3.67 GGGVATFSTSSLSAGTHH486 pKa = 5.8 SLTAVYY492 pKa = 10.44 AGDD495 pKa = 3.71 GSFNGSTSPVDD506 pKa = 3.76 TQTVTAANTTTALTSSPDD524 pKa = 3.37 PSVFGQAKK532 pKa = 9.82 VLTATVTAVAPGAGVPTGTVTFFDD556 pKa = 4.19 GMTSLGTGTLDD567 pKa = 3.36 GSGVATLSVSNLSVGAHH584 pKa = 6.22 SLTAGYY590 pKa = 10.69 AGTADD595 pKa = 4.09 YY596 pKa = 11.17 NSSTSAVDD604 pKa = 3.57 SQTVTAANTTTALTSSPDD622 pKa = 3.37 PSVFGQAKK630 pKa = 9.71 VLTATVSAVAPGAGVPTGTVTFFDD654 pKa = 4.19 GMTSLGTGTLNGSGVATLSVSNLSVGAHH682 pKa = 6.22 SLTAGYY688 pKa = 10.69 AGTADD693 pKa = 4.09 YY694 pKa = 11.17 NSSTSAVDD702 pKa = 3.57 SQTVTAANTTTALTSSPDD720 pKa = 3.37 PSVFGQAKK728 pKa = 9.71 VLTATVSAVAPGAGVPTGTVTFFDD752 pKa = 4.19 GMTSLGTGTLNGSGVATLSVSNLSVGAHH780 pKa = 6.22 SLTAGYY786 pKa = 10.69 AGTADD791 pKa = 4.09 YY792 pKa = 11.17 NSSTSAVDD800 pKa = 3.57 SQTVTAANTTTALTSSPDD818 pKa = 3.38 PSVFGQGKK826 pKa = 9.01 VLTATVSAVAPGAGVPTGTVTFFDD850 pKa = 4.19 GMTSLGTGILVGSGVATLSVSNLSVGSHH878 pKa = 6.86 ALTAAYY884 pKa = 10.05 GGDD887 pKa = 3.13 TSYY890 pKa = 11.55 NGSTSPIDD898 pKa = 3.69 TQTVNAADD906 pKa = 3.74 TATALTSSPDD916 pKa = 3.29 PSVFGEE922 pKa = 4.14 AKK924 pKa = 10.44 VLTATVSAVAPGAGVPTGTVTFFDD948 pKa = 4.19 GMTSLGTGILNGSGVATLSVSNLSVGSHH976 pKa = 6.86 ALTAAYY982 pKa = 10.05 GGDD985 pKa = 3.13 TSYY988 pKa = 11.55 NGSTSPIDD996 pKa = 3.69 TQTVNAADD1004 pKa = 3.74 TTTALTSSPDD1014 pKa = 3.29 PSVFGEE1020 pKa = 4.45 TKK1022 pKa = 10.61 VLTATVTPVAPGTGVPTGTVTFFDD1046 pKa = 4.19 GMTSLGTGTLDD1057 pKa = 3.36 GSGVATLSVSNLSVGTHH1074 pKa = 7.12 PLTAAYY1080 pKa = 10.06 GGDD1083 pKa = 3.13 TSYY1086 pKa = 11.52 NGSTSPVDD1094 pKa = 3.66 TQTVNAADD1102 pKa = 3.74 TATALTSSPDD1112 pKa = 3.29 PSVFGEE1118 pKa = 4.14 AKK1120 pKa = 10.48 VLTATVTAVAPGSGTPTGSVSFLDD1144 pKa = 3.99 GATVIGIGTLDD1155 pKa = 3.67 GGGVATFTTSSLSAGTHH1172 pKa = 5.8 SLTAVYY1178 pKa = 10.44 AGDD1181 pKa = 3.71 GSFNGSTSPVDD1192 pKa = 3.66 TQTVNAANTTTALTSSPDD1210 pKa = 3.29 PSVFGEE1216 pKa = 4.14 AKK1218 pKa = 10.44 VLTATVSAVAPGAGVPTGTVTFFDD1242 pKa = 4.19 GMTSLGTGTLDD1253 pKa = 3.36 GSGVATLSVSNLSVGSHH1270 pKa = 6.86 ALTAAYY1276 pKa = 10.05 GGDD1279 pKa = 3.13 TSYY1282 pKa = 11.55 NGSTSPIDD1290 pKa = 3.69 TQTVNAADD1298 pKa = 3.74 TATALTSSPDD1308 pKa = 3.29 PSVFGEE1314 pKa = 4.14 AKK1316 pKa = 10.44 VLTATVSAVAPGAGVPTGTVTFFDD1340 pKa = 4.19 GMTSLGTGILDD1351 pKa = 3.69 GSGVATLSVSNLSVGTHH1368 pKa = 7.12 PLTAAYY1374 pKa = 10.06 GGDD1377 pKa = 3.13 TSYY1380 pKa = 11.55 NGSTSPIDD1388 pKa = 3.69 TQTVNAANTTTALTSSPDD1406 pKa = 3.29 PSVFGEE1412 pKa = 4.45 TKK1414 pKa = 10.61 VLTATVTPVAPGTGVPTGTVTFFDD1438 pKa = 4.19 GMTSLGTGTLDD1449 pKa = 3.36 GSGVATLSVSNLSVGTHH1466 pKa = 7.12 PLTAAYY1472 pKa = 10.06 GGDD1475 pKa = 3.13 TSYY1478 pKa = 11.52 NGSTSPVDD1486 pKa = 3.66 TQTVNAADD1494 pKa = 3.74 TATALTSSPDD1504 pKa = 3.29 PSVFGEE1510 pKa = 4.14 AKK1512 pKa = 10.48 VLTATVTAVAPGSGTPTGSVSFLDD1536 pKa = 3.99 GATVIGIGTLDD1547 pKa = 3.67 GGGVATFTTSSLSAGTHH1564 pKa = 5.8 SLTAVYY1570 pKa = 10.44 AGDD1573 pKa = 3.71 GSFNGSTSPVDD1584 pKa = 3.66 TQTVNAANTTTALTSSPDD1602 pKa = 3.29 PSVFGEE1608 pKa = 4.14 AKK1610 pKa = 10.44 VLTATVSAVAPGAGVPTGTVTFFDD1634 pKa = 4.19 GMTSLGTGTLDD1645 pKa = 3.36 GSGVATLSVSNLSVGTHH1662 pKa = 7.12 PLTAAYY1668 pKa = 10.06 GGDD1671 pKa = 3.13 TSYY1674 pKa = 11.58 NGSTSPTDD1682 pKa = 3.51 TQTVNAADD1690 pKa = 3.74 TTTALTSSPDD1700 pKa = 3.36 PSVFGQTKK1708 pKa = 9.87 VLTATVSAVAPGAGVPTGTVTFFDD1732 pKa = 4.19 GMTSLGTGTLDD1743 pKa = 3.4 GTGVATLSVSNLSVGTHH1760 pKa = 7.05 PLTATYY1766 pKa = 10.72 AGDD1769 pKa = 3.34 TSYY1772 pKa = 11.5 NGSTSPIDD1780 pKa = 3.69 TQTVNAADD1788 pKa = 3.74 TATTLTSSPDD1798 pKa = 3.31 PSVFGQAKK1806 pKa = 9.71 VLTATVSPVAPGGGIPTGTVTFFDD1830 pKa = 4.19 GMTSLGTGTLDD1841 pKa = 3.4 GTGVATLSVSNLSVGTHH1858 pKa = 7.09 PLTATYY1864 pKa = 10.67 GGDD1867 pKa = 3.25 SSFNGSTSPADD1878 pKa = 3.71 TQTVNAANTTTALTSSPDD1896 pKa = 3.38 PSVFGQGKK1904 pKa = 9.01 VLTATVSPVAPGGGIPTGTVTFFDD1928 pKa = 4.19 GMTSLGTGTLDD1939 pKa = 3.36 GSGVATLTVSNLSVGSHH1956 pKa = 6.8 ALTATYY1962 pKa = 10.6 GGDD1965 pKa = 3.25 SSFNGSTSPADD1976 pKa = 3.71 TQTVNAANTTTTLTSSPDD1994 pKa = 3.23 PSVFGEE2000 pKa = 4.14 AKK2002 pKa = 10.48 VLTATVTAVAPGSGTPTGSVSFLDD2026 pKa = 3.99 GATVIGIGTLDD2037 pKa = 3.67 GGGVATFTTSTLGAGTHH2054 pKa = 6.08 SLTAVYY2060 pKa = 10.44 AGDD2063 pKa = 3.71 GSFNGSTSPVDD2074 pKa = 3.66 TQTVNAANTTTALTSSPDD2092 pKa = 3.38 PSVFGQGKK2100 pKa = 9.01 VLTATVSPVAPGGGIPTGTVTFFDD2124 pKa = 4.19 GMTSLGTGTLNGSGVATLTVSNLSVGSHH2152 pKa = 6.18 SLTATYY2158 pKa = 10.66 GGDD2161 pKa = 3.25 SSFNGSTSPADD2172 pKa = 3.71 TQTVNAADD2180 pKa = 3.74 TTTALTSSPDD2190 pKa = 3.36 PSVFGQTKK2198 pKa = 9.87 VLTATVSAVAPGAGVPTGTVTFFDD2222 pKa = 4.19 GMTSLGTGTLDD2233 pKa = 3.4 GTGVATLSVSNLSVGTHH2250 pKa = 7.09 PLTATYY2256 pKa = 10.63 GGDD2259 pKa = 3.01 TSYY2262 pKa = 11.59 NGSTSPADD2270 pKa = 3.58 TQTVNTANTTATLTSSPDD2288 pKa = 3.29 PSVFGQTKK2296 pKa = 10.04 VLTATVTPVAPGTGVPTGTVTFFDD2320 pKa = 4.19 GMTSLGTGTLDD2331 pKa = 3.36 GSGVATLSVSNLSVGTHH2348 pKa = 7.12 PLTAAYY2354 pKa = 10.11 GGDD2357 pKa = 3.49 TSHH2360 pKa = 6.95 NGSTSPIDD2368 pKa = 3.69 TQTVNAANTTTALTSSPDD2386 pKa = 3.36 PSVFGQTKK2394 pKa = 10.04 VLTATVTPVAPGTGVPTGTVTFFDD2418 pKa = 4.19 GMTSLGTGTLNGSGVATLSVSNLSVGTHH2446 pKa = 7.12 PLTAAYY2452 pKa = 10.11 GGDD2455 pKa = 3.49 TSHH2458 pKa = 6.94 NGSTSPVDD2466 pKa = 3.4 TQTVNKK2472 pKa = 10.28 ANTTTAVSSSPNPSVFGQSVNLTATVAVVAPGGGVPGGTVTFFVGGIPQTPAVPLNGSGVATLSISTLSVGTRR2545 pKa = 11.84 SVRR2548 pKa = 11.84 ASYY2551 pKa = 10.65 NGSAGYY2557 pKa = 7.79 NTSTSPTISQVVSKK2571 pKa = 11.02 ANTSTTLTSSPDD2583 pKa = 3.31 PSVFGQTKK2591 pKa = 9.87 VLTATVSPVAPGGGTPIGTVSFFDD2615 pKa = 3.94 GMTLLGTGTLSGGVATLSTSALALGSHH2642 pKa = 6.51 SLTATYY2648 pKa = 10.36 GGSANFNGSTSPVDD2662 pKa = 3.62 TQTVNPANTTTVLTSSPDD2680 pKa = 3.51 PSVLGQAKK2688 pKa = 10.02 VLTATVSATAPGAGTPSGTVTFFDD2712 pKa = 4.26 GMTSLGTGALDD2723 pKa = 3.79 GSGVATLSVSNLSVGSHH2740 pKa = 5.89 TLTATYY2746 pKa = 10.29 GGSANFNGSTSAVDD2760 pKa = 3.58 TQTVNPANTMTALTSSPDD2778 pKa = 3.49 PSVTGQAKK2786 pKa = 10.08 VLTATVTAVAPGTGTPSGSVTFFDD2810 pKa = 5.01 GMTSLGTGTLDD2821 pKa = 3.36 GSGVATLSVSNLSVGNHH2838 pKa = 6.07 SLTAAYY2844 pKa = 9.67 GGSGSYY2850 pKa = 10.26 NGSTSSADD2858 pKa = 3.31 TQTVNKK2864 pKa = 10.27 ANTTTAVSSSPNPSVFGQSVNLTATVAVVAPGGGVPGGTVTFFIGGIPQTPAVPLNGSGVATLPISTLSVGTRR2937 pKa = 11.84 SVRR2940 pKa = 11.84 ASYY2943 pKa = 10.65 NGSAGYY2949 pKa = 7.79 NTSTSPTISQVVSKK2963 pKa = 11.02 ANTSTTLVSAPNPSMSGQSVTLTATVAPVAPGGGVPTGSVSFLDD3007 pKa = 4.3 GMTLLGTGTLSGGVATFSTSSLSVGSHH3034 pKa = 6.1 SLTAAYY3040 pKa = 9.84 AGSGSYY3046 pKa = 10.23 NGSTSPAVTQTVSS3059 pKa = 2.63
Molecular weight: 294.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.656
IPC_protein 3.745
Toseland 3.49
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.757
Rodwell 3.567
Grimsley 3.389
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.24
Thurlkill 3.554
EMBOSS 3.757
Sillero 3.872
Patrickios 0.795
IPC_peptide 3.757
IPC2_peptide 3.846
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A3N4SG66|A0A3N4SG66_9ACTN Uncharacterized protein OS=Streptomyces sp. Ag109_O5-1 OX=1938851 GN=EDD90_5732 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.46 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10263
0
10263
3383640
29
10866
329.7
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.353 ± 0.035
0.805 ± 0.007
5.968 ± 0.019
5.445 ± 0.026
2.754 ± 0.014
9.323 ± 0.024
2.376 ± 0.015
3.201 ± 0.017
2.181 ± 0.023
10.246 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.702 ± 0.01
1.893 ± 0.013
6.032 ± 0.021
2.895 ± 0.017
7.889 ± 0.031
5.271 ± 0.022
6.434 ± 0.028
8.459 ± 0.043
1.582 ± 0.01
2.191 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here