Streptomyces sp. Ag109_O5-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3N4S336|A0A3N4S336_9ACTN Uncharacterized protein OS=Streptomyces sp. Ag109_O5-1 OX=1938851 GN=EDD90_2844 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.23AFQSRR8 pKa = 11.84WRR10 pKa = 11.84QLLGIGRR17 pKa = 11.84NTARR21 pKa = 11.84RR22 pKa = 11.84VAAALLLDD30 pKa = 3.53PTVPVGNFPTGVAINSAGTLAYY52 pKa = 9.78VANEE56 pKa = 4.06GDD58 pKa = 3.98DD59 pKa = 3.86SVSVIDD65 pKa = 3.69TATNAVIATINVGSSPFGVAVTPDD89 pKa = 3.13GAFVYY94 pKa = 8.04VTNVASDD101 pKa = 3.8SVSVIDD107 pKa = 3.7TATNAVTDD115 pKa = 4.13TVSVGISPFGIAINAAGTLAYY136 pKa = 9.49VANVGDD142 pKa = 3.9NTVSVIDD149 pKa = 3.74TSTNTVVTDD158 pKa = 3.6IPVGANPFGVALTPDD173 pKa = 3.19GAFAYY178 pKa = 7.53VTNNASGTVSVVDD191 pKa = 3.42TATNAEE197 pKa = 4.12IDD199 pKa = 4.05TIAVGTSPTGVAITSDD215 pKa = 3.3GVLAYY220 pKa = 8.19VTNNSDD226 pKa = 3.52NTVSAIDD233 pKa = 3.36IATNTVSDD241 pKa = 3.84TFDD244 pKa = 3.15VGSAPTSVAFTPDD257 pKa = 3.07DD258 pKa = 3.48AFAYY262 pKa = 7.0VTNNASDD269 pKa = 3.77TVSVVITATNDD280 pKa = 2.96VTFDD284 pKa = 3.47IPVGSGPFGVAITPDD299 pKa = 3.63GEE301 pKa = 4.21HH302 pKa = 7.3AYY304 pKa = 8.65VTNNASDD311 pKa = 3.97TVSVTDD317 pKa = 3.51TVPFPTTTTLTSAPDD332 pKa = 3.55PSAFGEE338 pKa = 4.27AKK340 pKa = 10.54VLTATVTSSTGTPDD354 pKa = 3.14GTVTFLDD361 pKa = 4.29GATPLGTVTLAGGVATLTTSSLAVGSHH388 pKa = 6.26SLTADD393 pKa = 3.2YY394 pKa = 10.95AGNVNFSVSTSAADD408 pKa = 3.3IQTVNAADD416 pKa = 3.69TATTLTSSPDD426 pKa = 3.23PSVFGEE432 pKa = 4.14AKK434 pKa = 10.48VLTATVTAVAPGSGTPTGSVSFLDD458 pKa = 3.99GATVIGIGTLDD469 pKa = 3.67GGGVATFSTSSLSAGTHH486 pKa = 5.8SLTAVYY492 pKa = 10.44AGDD495 pKa = 3.71GSFNGSTSPVDD506 pKa = 3.76TQTVTAANTTTALTSSPDD524 pKa = 3.37PSVFGQAKK532 pKa = 9.82VLTATVTAVAPGAGVPTGTVTFFDD556 pKa = 4.19GMTSLGTGTLDD567 pKa = 3.36GSGVATLSVSNLSVGAHH584 pKa = 6.22SLTAGYY590 pKa = 10.69AGTADD595 pKa = 4.09YY596 pKa = 11.17NSSTSAVDD604 pKa = 3.57SQTVTAANTTTALTSSPDD622 pKa = 3.37PSVFGQAKK630 pKa = 9.71VLTATVSAVAPGAGVPTGTVTFFDD654 pKa = 4.19GMTSLGTGTLNGSGVATLSVSNLSVGAHH682 pKa = 6.22SLTAGYY688 pKa = 10.69AGTADD693 pKa = 4.09YY694 pKa = 11.17NSSTSAVDD702 pKa = 3.57SQTVTAANTTTALTSSPDD720 pKa = 3.37PSVFGQAKK728 pKa = 9.71VLTATVSAVAPGAGVPTGTVTFFDD752 pKa = 4.19GMTSLGTGTLNGSGVATLSVSNLSVGAHH780 pKa = 6.22SLTAGYY786 pKa = 10.69AGTADD791 pKa = 4.09YY792 pKa = 11.17NSSTSAVDD800 pKa = 3.57SQTVTAANTTTALTSSPDD818 pKa = 3.38PSVFGQGKK826 pKa = 9.01VLTATVSAVAPGAGVPTGTVTFFDD850 pKa = 4.19GMTSLGTGILVGSGVATLSVSNLSVGSHH878 pKa = 6.86ALTAAYY884 pKa = 10.05GGDD887 pKa = 3.13TSYY890 pKa = 11.55NGSTSPIDD898 pKa = 3.69TQTVNAADD906 pKa = 3.74TATALTSSPDD916 pKa = 3.29PSVFGEE922 pKa = 4.14AKK924 pKa = 10.44VLTATVSAVAPGAGVPTGTVTFFDD948 pKa = 4.19GMTSLGTGILNGSGVATLSVSNLSVGSHH976 pKa = 6.86ALTAAYY982 pKa = 10.05GGDD985 pKa = 3.13TSYY988 pKa = 11.55NGSTSPIDD996 pKa = 3.69TQTVNAADD1004 pKa = 3.74TTTALTSSPDD1014 pKa = 3.29PSVFGEE1020 pKa = 4.45TKK1022 pKa = 10.61VLTATVTPVAPGTGVPTGTVTFFDD1046 pKa = 4.19GMTSLGTGTLDD1057 pKa = 3.36GSGVATLSVSNLSVGTHH1074 pKa = 7.12PLTAAYY1080 pKa = 10.06GGDD1083 pKa = 3.13TSYY1086 pKa = 11.52NGSTSPVDD1094 pKa = 3.66TQTVNAADD1102 pKa = 3.74TATALTSSPDD1112 pKa = 3.29PSVFGEE1118 pKa = 4.14AKK1120 pKa = 10.48VLTATVTAVAPGSGTPTGSVSFLDD1144 pKa = 3.99GATVIGIGTLDD1155 pKa = 3.67GGGVATFTTSSLSAGTHH1172 pKa = 5.8SLTAVYY1178 pKa = 10.44AGDD1181 pKa = 3.71GSFNGSTSPVDD1192 pKa = 3.66TQTVNAANTTTALTSSPDD1210 pKa = 3.29PSVFGEE1216 pKa = 4.14AKK1218 pKa = 10.44VLTATVSAVAPGAGVPTGTVTFFDD1242 pKa = 4.19GMTSLGTGTLDD1253 pKa = 3.36GSGVATLSVSNLSVGSHH1270 pKa = 6.86ALTAAYY1276 pKa = 10.05GGDD1279 pKa = 3.13TSYY1282 pKa = 11.55NGSTSPIDD1290 pKa = 3.69TQTVNAADD1298 pKa = 3.74TATALTSSPDD1308 pKa = 3.29PSVFGEE1314 pKa = 4.14AKK1316 pKa = 10.44VLTATVSAVAPGAGVPTGTVTFFDD1340 pKa = 4.19GMTSLGTGILDD1351 pKa = 3.69GSGVATLSVSNLSVGTHH1368 pKa = 7.12PLTAAYY1374 pKa = 10.06GGDD1377 pKa = 3.13TSYY1380 pKa = 11.55NGSTSPIDD1388 pKa = 3.69TQTVNAANTTTALTSSPDD1406 pKa = 3.29PSVFGEE1412 pKa = 4.45TKK1414 pKa = 10.61VLTATVTPVAPGTGVPTGTVTFFDD1438 pKa = 4.19GMTSLGTGTLDD1449 pKa = 3.36GSGVATLSVSNLSVGTHH1466 pKa = 7.12PLTAAYY1472 pKa = 10.06GGDD1475 pKa = 3.13TSYY1478 pKa = 11.52NGSTSPVDD1486 pKa = 3.66TQTVNAADD1494 pKa = 3.74TATALTSSPDD1504 pKa = 3.29PSVFGEE1510 pKa = 4.14AKK1512 pKa = 10.48VLTATVTAVAPGSGTPTGSVSFLDD1536 pKa = 3.99GATVIGIGTLDD1547 pKa = 3.67GGGVATFTTSSLSAGTHH1564 pKa = 5.8SLTAVYY1570 pKa = 10.44AGDD1573 pKa = 3.71GSFNGSTSPVDD1584 pKa = 3.66TQTVNAANTTTALTSSPDD1602 pKa = 3.29PSVFGEE1608 pKa = 4.14AKK1610 pKa = 10.44VLTATVSAVAPGAGVPTGTVTFFDD1634 pKa = 4.19GMTSLGTGTLDD1645 pKa = 3.36GSGVATLSVSNLSVGTHH1662 pKa = 7.12PLTAAYY1668 pKa = 10.06GGDD1671 pKa = 3.13TSYY1674 pKa = 11.58NGSTSPTDD1682 pKa = 3.51TQTVNAADD1690 pKa = 3.74TTTALTSSPDD1700 pKa = 3.36PSVFGQTKK1708 pKa = 9.87VLTATVSAVAPGAGVPTGTVTFFDD1732 pKa = 4.19GMTSLGTGTLDD1743 pKa = 3.4GTGVATLSVSNLSVGTHH1760 pKa = 7.05PLTATYY1766 pKa = 10.72AGDD1769 pKa = 3.34TSYY1772 pKa = 11.5NGSTSPIDD1780 pKa = 3.69TQTVNAADD1788 pKa = 3.74TATTLTSSPDD1798 pKa = 3.31PSVFGQAKK1806 pKa = 9.71VLTATVSPVAPGGGIPTGTVTFFDD1830 pKa = 4.19GMTSLGTGTLDD1841 pKa = 3.4GTGVATLSVSNLSVGTHH1858 pKa = 7.09PLTATYY1864 pKa = 10.67GGDD1867 pKa = 3.25SSFNGSTSPADD1878 pKa = 3.71TQTVNAANTTTALTSSPDD1896 pKa = 3.38PSVFGQGKK1904 pKa = 9.01VLTATVSPVAPGGGIPTGTVTFFDD1928 pKa = 4.19GMTSLGTGTLDD1939 pKa = 3.36GSGVATLTVSNLSVGSHH1956 pKa = 6.8ALTATYY1962 pKa = 10.6GGDD1965 pKa = 3.25SSFNGSTSPADD1976 pKa = 3.71TQTVNAANTTTTLTSSPDD1994 pKa = 3.23PSVFGEE2000 pKa = 4.14AKK2002 pKa = 10.48VLTATVTAVAPGSGTPTGSVSFLDD2026 pKa = 3.99GATVIGIGTLDD2037 pKa = 3.67GGGVATFTTSTLGAGTHH2054 pKa = 6.08SLTAVYY2060 pKa = 10.44AGDD2063 pKa = 3.71GSFNGSTSPVDD2074 pKa = 3.66TQTVNAANTTTALTSSPDD2092 pKa = 3.38PSVFGQGKK2100 pKa = 9.01VLTATVSPVAPGGGIPTGTVTFFDD2124 pKa = 4.19GMTSLGTGTLNGSGVATLTVSNLSVGSHH2152 pKa = 6.18SLTATYY2158 pKa = 10.66GGDD2161 pKa = 3.25SSFNGSTSPADD2172 pKa = 3.71TQTVNAADD2180 pKa = 3.74TTTALTSSPDD2190 pKa = 3.36PSVFGQTKK2198 pKa = 9.87VLTATVSAVAPGAGVPTGTVTFFDD2222 pKa = 4.19GMTSLGTGTLDD2233 pKa = 3.4GTGVATLSVSNLSVGTHH2250 pKa = 7.09PLTATYY2256 pKa = 10.63GGDD2259 pKa = 3.01TSYY2262 pKa = 11.59NGSTSPADD2270 pKa = 3.58TQTVNTANTTATLTSSPDD2288 pKa = 3.29PSVFGQTKK2296 pKa = 10.04VLTATVTPVAPGTGVPTGTVTFFDD2320 pKa = 4.19GMTSLGTGTLDD2331 pKa = 3.36GSGVATLSVSNLSVGTHH2348 pKa = 7.12PLTAAYY2354 pKa = 10.11GGDD2357 pKa = 3.49TSHH2360 pKa = 6.95NGSTSPIDD2368 pKa = 3.69TQTVNAANTTTALTSSPDD2386 pKa = 3.36PSVFGQTKK2394 pKa = 10.04VLTATVTPVAPGTGVPTGTVTFFDD2418 pKa = 4.19GMTSLGTGTLNGSGVATLSVSNLSVGTHH2446 pKa = 7.12PLTAAYY2452 pKa = 10.11GGDD2455 pKa = 3.49TSHH2458 pKa = 6.94NGSTSPVDD2466 pKa = 3.4TQTVNKK2472 pKa = 10.28ANTTTAVSSSPNPSVFGQSVNLTATVAVVAPGGGVPGGTVTFFVGGIPQTPAVPLNGSGVATLSISTLSVGTRR2545 pKa = 11.84SVRR2548 pKa = 11.84ASYY2551 pKa = 10.65NGSAGYY2557 pKa = 7.79NTSTSPTISQVVSKK2571 pKa = 11.02ANTSTTLTSSPDD2583 pKa = 3.31PSVFGQTKK2591 pKa = 9.87VLTATVSPVAPGGGTPIGTVSFFDD2615 pKa = 3.94GMTLLGTGTLSGGVATLSTSALALGSHH2642 pKa = 6.51SLTATYY2648 pKa = 10.36GGSANFNGSTSPVDD2662 pKa = 3.62TQTVNPANTTTVLTSSPDD2680 pKa = 3.51PSVLGQAKK2688 pKa = 10.02VLTATVSATAPGAGTPSGTVTFFDD2712 pKa = 4.26GMTSLGTGALDD2723 pKa = 3.79GSGVATLSVSNLSVGSHH2740 pKa = 5.89TLTATYY2746 pKa = 10.29GGSANFNGSTSAVDD2760 pKa = 3.58TQTVNPANTMTALTSSPDD2778 pKa = 3.49PSVTGQAKK2786 pKa = 10.08VLTATVTAVAPGTGTPSGSVTFFDD2810 pKa = 5.01GMTSLGTGTLDD2821 pKa = 3.36GSGVATLSVSNLSVGNHH2838 pKa = 6.07SLTAAYY2844 pKa = 9.67GGSGSYY2850 pKa = 10.26NGSTSSADD2858 pKa = 3.31TQTVNKK2864 pKa = 10.27ANTTTAVSSSPNPSVFGQSVNLTATVAVVAPGGGVPGGTVTFFIGGIPQTPAVPLNGSGVATLPISTLSVGTRR2937 pKa = 11.84SVRR2940 pKa = 11.84ASYY2943 pKa = 10.65NGSAGYY2949 pKa = 7.79NTSTSPTISQVVSKK2963 pKa = 11.02ANTSTTLVSAPNPSMSGQSVTLTATVAPVAPGGGVPTGSVSFLDD3007 pKa = 4.3GMTLLGTGTLSGGVATFSTSSLSVGSHH3034 pKa = 6.1SLTAAYY3040 pKa = 9.84AGSGSYY3046 pKa = 10.23NGSTSPAVTQTVSS3059 pKa = 2.63

Molecular weight:
294.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3N4SG66|A0A3N4SG66_9ACTN Uncharacterized protein OS=Streptomyces sp. Ag109_O5-1 OX=1938851 GN=EDD90_5732 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight:
5.22 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10263

0

10263

3383640

29

10866

329.7

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.353 ± 0.035

0.805 ± 0.007

5.968 ± 0.019

5.445 ± 0.026

2.754 ± 0.014

9.323 ± 0.024

2.376 ± 0.015

3.201 ± 0.017

2.181 ± 0.023

10.246 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.702 ± 0.01

1.893 ± 0.013

6.032 ± 0.021

2.895 ± 0.017

7.889 ± 0.031

5.271 ± 0.022

6.434 ± 0.028

8.459 ± 0.043

1.582 ± 0.01

2.191 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski