Lacibacter luteus
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4180 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q1CNH8|A0A4Q1CNH8_9BACT Beta-glucosidase OS=Lacibacter luteus OX=2508719 GN=ESA94_04415 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.46 QFLPYY7 pKa = 9.97 KK8 pKa = 8.45 RR9 pKa = 11.84 NCMGAAIKK17 pKa = 10.24 NLHH20 pKa = 6.56 LLFTLSIALFVSTNVANAQCTCTGNIVVNPSFEE53 pKa = 4.39 SGTTGWSWSGGSFNAGTGAIACGSKK78 pKa = 10.49 SGDD81 pKa = 3.42 FQISNTSSNWVSQTIGTDD99 pKa = 3.72 LAPGTKK105 pKa = 9.47 INASVYY111 pKa = 10.77 AGTHH115 pKa = 5.07 NNSYY119 pKa = 8.75 YY120 pKa = 10.35 HH121 pKa = 5.9 QVAIDD126 pKa = 3.92 FFDD129 pKa = 5.82 ANWNWISNSKK139 pKa = 10.38 VEE141 pKa = 4.21 VNKK144 pKa = 10.1 VLSTSPVGPQLYY156 pKa = 8.27 TWSGTVPVGTKK167 pKa = 7.39 YY168 pKa = 9.61 TNVSFSGNGDD178 pKa = 3.84 WIKK181 pKa = 10.2 TDD183 pKa = 3.21 QWCVTLTPPAGGSIGDD199 pKa = 4.23 RR200 pKa = 11.84 VWLDD204 pKa = 3.06 ADD206 pKa = 4.07 GDD208 pKa = 4.27 GLQDD212 pKa = 3.4 ASEE215 pKa = 4.26 TSGIAGVTVQLKK227 pKa = 10.25 NSLGSVIATTTTNASGNYY245 pKa = 9.76 LFTGLAAGNYY255 pKa = 6.93 TVVFPTSISGAVVTPQNVGLDD276 pKa = 3.97 DD277 pKa = 4.96 NVDD280 pKa = 3.47 SDD282 pKa = 4.49 ASQTTGATSTITLATNQNITNVDD305 pKa = 3.06 AGYY308 pKa = 10.83 CPTTLSLGNRR318 pKa = 11.84 VWYY321 pKa = 7.73 DD322 pKa = 3.48 TNNDD326 pKa = 4.14 GINSNEE332 pKa = 3.8 NGIPNLTVYY341 pKa = 10.55 LYY343 pKa = 11.09 KK344 pKa = 10.48 DD345 pKa = 3.74 DD346 pKa = 5.55 NNDD349 pKa = 3.19 NVADD353 pKa = 3.93 AAAIATAVTDD363 pKa = 3.82 ANGYY367 pKa = 10.47 YY368 pKa = 9.01 MFSNLAPGNYY378 pKa = 8.66 IVGAVTPAGYY388 pKa = 9.23 MSSLVNGGDD397 pKa = 3.46 PDD399 pKa = 5.01 DD400 pKa = 5.17 NNNSDD405 pKa = 4.55 DD406 pKa = 3.97 NGEE409 pKa = 4.24 VTVGAEE415 pKa = 4.05 TRR417 pKa = 11.84 GLAITLVAGTEE428 pKa = 3.99 PLVDD432 pKa = 3.89 GNTNSNSNITYY443 pKa = 10.52 DD444 pKa = 4.2 FGFLPDD450 pKa = 4.37 CNCTSSSSNLLINGSFEE467 pKa = 4.18 NGTTGWSASGGSVSSGTGYY486 pKa = 9.57 VACGAANGFNSWSSGTSRR504 pKa = 11.84 VWQDD508 pKa = 2.87 VAVAAGSTVVFKK520 pKa = 11.02 GFAGVHH526 pKa = 4.68 TAGLTCSPKK535 pKa = 10.61 LSLIFYY541 pKa = 9.14 NSSNTVISQNDD552 pKa = 3.44 VVVTRR557 pKa = 11.84 DD558 pKa = 2.69 VDD560 pKa = 3.52 KK561 pKa = 11.62 YY562 pKa = 10.68 FGQLEE567 pKa = 4.44 QYY569 pKa = 10.42 SITAVAPAGTVKK581 pKa = 10.25 VRR583 pKa = 11.84 VQSAITCNYY592 pKa = 7.32 MKK594 pKa = 10.7 LDD596 pKa = 4.12 ALCLTATAPLALGDD610 pKa = 4.32 RR611 pKa = 11.84 VFFDD615 pKa = 4.9 LNDD618 pKa = 3.21 NGTRR622 pKa = 11.84 DD623 pKa = 3.55 GSEE626 pKa = 3.68 TGLAGVTVKK635 pKa = 10.48 LYY637 pKa = 11.12 ADD639 pKa = 3.83 NNNDD643 pKa = 3.62 NIADD647 pKa = 4.08 GAALQTTTTDD657 pKa = 2.77 ANGFYY662 pKa = 10.5 RR663 pKa = 11.84 FSNLTAGNYY672 pKa = 8.73 IVGVVVPAGYY682 pKa = 10.07 RR683 pKa = 11.84 SSTLTGVDD691 pKa = 3.23 PDD693 pKa = 4.25 NNVDD697 pKa = 5.66 LDD699 pKa = 4.37 DD700 pKa = 4.81 NGNNTLISGEE710 pKa = 3.88 IRR712 pKa = 11.84 GNAVTLSAGSEE723 pKa = 4.01 PQEE726 pKa = 3.91 SGNFNNTYY734 pKa = 11.03 DD735 pKa = 3.87 FGFTGTGSIGDD746 pKa = 3.96 FVWNDD751 pKa = 2.98 LNANGIQDD759 pKa = 3.58 AGEE762 pKa = 4.59 PGLSGVTVTLTYY774 pKa = 10.48 PGGTTVTATTDD785 pKa = 3.12 ANGAYY790 pKa = 9.54 QFNNLPPGTYY800 pKa = 9.71 SISFSTPSGYY810 pKa = 9.81 IAGSANQGADD820 pKa = 3.58 DD821 pKa = 5.22 AKK823 pKa = 11.26 DD824 pKa = 3.49 SDD826 pKa = 4.47 PVSGTVSGIVLTAGQNNTTIDD847 pKa = 3.26 AGFVNNQLVLGNFVWYY863 pKa = 9.94 DD864 pKa = 3.22 KK865 pKa = 11.56 DD866 pKa = 3.59 NDD868 pKa = 3.97 GVQDD872 pKa = 3.57 AGEE875 pKa = 4.13 PGIAGVTVNLYY886 pKa = 10.68 KK887 pKa = 10.72 DD888 pKa = 3.69 ANGDD892 pKa = 3.93 NIADD896 pKa = 4.06 GAAVATTTTNASGLYY911 pKa = 10.22 SFTGLTAGNYY921 pKa = 8.63 IVGVVLPSGYY931 pKa = 10.16 AASAVSSATPNNDD944 pKa = 2.47 VNTDD948 pKa = 3.41 NNGVTTASGEE958 pKa = 4.09 VRR960 pKa = 11.84 SNYY963 pKa = 8.16 ITLSTAGEE971 pKa = 4.23 PTTDD975 pKa = 3.79 GDD977 pKa = 4.29 DD978 pKa = 3.74 ANGNLTLDD986 pKa = 3.78 FGLRR990 pKa = 11.84 GTASIGDD997 pKa = 4.17 YY998 pKa = 10.36 VWNDD1002 pKa = 3.21 LNGNGIQNTGEE1013 pKa = 4.26 PGLSGVTVTLTYY1025 pKa = 10.5 PDD1027 pKa = 3.87 GTTVTTTTDD1036 pKa = 2.77 GSGAYY1041 pKa = 10.08 LFANLAPGTYY1051 pKa = 9.48 SVAFATPNGSIASPSNAGADD1071 pKa = 3.79 DD1072 pKa = 4.22 TKK1074 pKa = 11.47 DD1075 pKa = 3.34 SDD1077 pKa = 4.05 PVSGVVSGITLTAGQTNTTIDD1098 pKa = 4.02 AGFQSNGLNLGNFVWYY1114 pKa = 9.76 DD1115 pKa = 3.42 RR1116 pKa = 11.84 NNNGVQDD1123 pKa = 3.74 AGEE1126 pKa = 4.19 PGIAGATVKK1135 pKa = 10.58 LYY1137 pKa = 11.18 ADD1139 pKa = 4.12 ANLDD1143 pKa = 4.04 NIADD1147 pKa = 4.14 GAALQTTTTDD1157 pKa = 2.98 ANGIYY1162 pKa = 9.92 SFTGLTPGNYY1172 pKa = 8.72 IAGVVLPAGYY1182 pKa = 9.66 AASSVSSSTPNNDD1195 pKa = 2.62 VNTDD1199 pKa = 3.45 NNGVTTTSGEE1209 pKa = 3.97 VRR1211 pKa = 11.84 SNYY1214 pKa = 8.04 ITLITASEE1222 pKa = 4.28 PTTDD1226 pKa = 3.77 GDD1228 pKa = 4.01 GSNGNLTLDD1237 pKa = 4.29 FGLKK1241 pKa = 8.67 GTGSIGDD1248 pKa = 3.92 FVWNDD1253 pKa = 3.34 LNGDD1257 pKa = 4.58 GIQNAGEE1264 pKa = 4.45 PGLSAVTVTLTYY1276 pKa = 10.34 PGGATATTTTDD1287 pKa = 2.93 ANGAYY1292 pKa = 9.87 QFTNLAPGTYY1302 pKa = 8.98 SVSFTTPSGYY1312 pKa = 9.66 IAAPANQGADD1322 pKa = 3.71 DD1323 pKa = 5.19 AKK1325 pKa = 11.26 DD1326 pKa = 3.49 SDD1328 pKa = 4.41 PVSGTVAGIVLTAGQTNTTIDD1349 pKa = 3.17 AGFVSNQLTLGNFVWYY1365 pKa = 9.89 DD1366 pKa = 3.22 KK1367 pKa = 11.56 DD1368 pKa = 3.59 NDD1370 pKa = 3.97 GVQDD1374 pKa = 3.68 AGEE1377 pKa = 4.19 LGIAAVTVNLYY1388 pKa = 10.7 KK1389 pKa = 10.68 DD1390 pKa = 3.68 ANGDD1394 pKa = 3.6 NVADD1398 pKa = 4.4 GAAVATTTTNASGIYY1413 pKa = 9.3 NFSGLTPGNYY1423 pKa = 8.78 IAGVVLPAGYY1433 pKa = 10.31 APSSVSSTTPNNDD1446 pKa = 2.35 INTDD1450 pKa = 3.51 NNGVTTAAGEE1460 pKa = 4.18 VRR1462 pKa = 11.84 SNYY1465 pKa = 8.26 ITLTTAGEE1473 pKa = 4.16 PTTDD1477 pKa = 3.29 GDD1479 pKa = 4.07 GSNGNLTLDD1488 pKa = 3.73 FGLRR1492 pKa = 11.84 GTASIGDD1499 pKa = 4.01 FVWNDD1504 pKa = 3.34 LNGDD1508 pKa = 4.65 GIQTAGEE1515 pKa = 3.91 PGIAGVTVTLTTAGGTVLEE1534 pKa = 4.64 TTTTNASGFYY1544 pKa = 9.96 QFSNLLPGTYY1554 pKa = 9.71 NVVFATPTGFVASAANAGADD1574 pKa = 3.82 DD1575 pKa = 4.62 TKK1577 pKa = 11.47 DD1578 pKa = 3.34 SDD1580 pKa = 4.25 PVSGTVTGVVLNADD1594 pKa = 3.93 EE1595 pKa = 4.79 NDD1597 pKa = 3.53 NTVDD1601 pKa = 5.54 AGFQSNQLKK1610 pKa = 9.54 VGNYY1614 pKa = 6.99 VWYY1617 pKa = 9.93 DD1618 pKa = 3.23 KK1619 pKa = 11.42 NNNGFQDD1626 pKa = 3.51 ADD1628 pKa = 3.53 EE1629 pKa = 4.4 TGIAGVSVKK1638 pKa = 10.74 LYY1640 pKa = 11.09 VDD1642 pKa = 4.11 ADD1644 pKa = 3.84 ANNIPDD1650 pKa = 3.77 AAAIASTTTDD1660 pKa = 2.99 ANGLYY1665 pKa = 9.9 SFSGLTPGNYY1675 pKa = 8.92 IVGITAPSGYY1685 pKa = 11.24 AMAFTTLSSLLPNNDD1700 pKa = 3.19 NNTDD1704 pKa = 3.56 NNGITIIGTEE1714 pKa = 3.68 ITSNYY1719 pKa = 7.36 ITLITNSEE1727 pKa = 4.26 PVTDD1731 pKa = 3.88 GDD1733 pKa = 4.39 GNNGNLTLDD1742 pKa = 4.21 FGLKK1746 pKa = 8.67 GTGSIGDD1753 pKa = 4.43 LVWDD1757 pKa = 4.67 DD1758 pKa = 4.79 LNANGKK1764 pKa = 9.13 QDD1766 pKa = 3.36 AGEE1769 pKa = 4.09 PGIAGVTVTLTYY1781 pKa = 10.33 PGGSTTSVITDD1792 pKa = 3.18 ATGTYY1797 pKa = 10.08 LFSNLAPGSYY1807 pKa = 9.83 SVSFDD1812 pKa = 3.26 KK1813 pKa = 11.0 PLGYY1817 pKa = 9.69 IEE1819 pKa = 4.08 TTANQVADD1827 pKa = 3.93 DD1828 pKa = 4.4 TKK1830 pKa = 11.37 DD1831 pKa = 3.23 SDD1833 pKa = 4.41 VISGSVSNVTLTAGQTNFTVDD1854 pKa = 2.67 AGYY1857 pKa = 10.76 YY1858 pKa = 9.26 KK1859 pKa = 10.22 PASIGDD1865 pKa = 3.92 FVWNDD1870 pKa = 2.98 LNANGIQDD1878 pKa = 3.55 AGEE1881 pKa = 4.11 PGIAGATVNLTGTDD1895 pKa = 3.27 ATGAAVNLTVSTEE1908 pKa = 3.88 ADD1910 pKa = 3.16 GSYY1913 pKa = 10.65 SFSSLLPGTYY1923 pKa = 9.32 NVSFVTPAGYY1933 pKa = 7.82 VTATSNAGADD1943 pKa = 3.81 DD1944 pKa = 4.36 TKK1946 pKa = 11.47 DD1947 pKa = 3.33 SDD1949 pKa = 4.24 PVSGAVNGITLISGQNNATVDD1970 pKa = 3.24 AGFIQQTPIVLSGHH1984 pKa = 5.79 VFYY1987 pKa = 10.79 DD1988 pKa = 3.77 DD1989 pKa = 4.81 NGMSDD1994 pKa = 3.55 GFVNGTSVIPSGINAVLYY2012 pKa = 10.59 DD2013 pKa = 4.2 PLTNTVVAVQPVPGYY2028 pKa = 8.42 GQPGAGTFSFNVLTYY2043 pKa = 8.32 STYY2046 pKa = 10.69 RR2047 pKa = 11.84 VLITTANPAVGSQAPVTSVLPASFRR2072 pKa = 11.84 STGEE2076 pKa = 4.14 TIGTAAGNDD2085 pKa = 3.62 GTIDD2089 pKa = 3.72 GKK2091 pKa = 11.12 SSIINTTNQNIIEE2104 pKa = 4.26 VNFAIKK2110 pKa = 10.02 RR2111 pKa = 11.84 VVLIVVDD2118 pKa = 4.03
Molecular weight: 216.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.541
IPC_protein 3.63
Toseland 3.376
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.274
Solomon 3.63
Lehninger 3.592
Nozaki 3.745
DTASelect 4.075
Thurlkill 3.427
EMBOSS 3.617
Sillero 3.745
Patrickios 0.312
IPC_peptide 3.63
IPC2_peptide 3.719
IPC2.peptide.svr19 3.695
Protein with the highest isoelectric point:
>tr|A0A4V1M7T8|A0A4V1M7T8_9BACT Alpha-E domain-containing protein OS=Lacibacter luteus OX=2508719 GN=ESA94_00335 PE=4 SV=1
MM1 pKa = 7.99 RR2 pKa = 11.84 SYY4 pKa = 10.71 FKK6 pKa = 10.46 QWTGMRR12 pKa = 11.84 LLRR15 pKa = 11.84 LLMGVVAIVQGIASPTPLLWVIGAVLLVQAFLNIGCMDD53 pKa = 4.36 SSCNIPVQQQKK64 pKa = 7.25 MHH66 pKa = 6.54 RR67 pKa = 3.77
Molecular weight: 7.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.545
IPC_protein 10.131
Toseland 10.467
ProMoST 10.833
Dawson 10.599
Bjellqvist 10.306
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.701
Lehninger 10.672
Nozaki 10.526
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.804
IPC_peptide 10.701
IPC2_peptide 9.677
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4180
0
4180
1521579
15
3128
364.0
40.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.746 ± 0.034
0.854 ± 0.013
5.036 ± 0.026
5.643 ± 0.038
5.035 ± 0.027
6.787 ± 0.037
1.829 ± 0.017
6.668 ± 0.029
7.036 ± 0.039
9.131 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.021
5.76 ± 0.04
3.726 ± 0.021
3.963 ± 0.023
3.863 ± 0.023
6.241 ± 0.032
6.185 ± 0.048
6.697 ± 0.025
1.375 ± 0.014
4.102 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here