Flavisolibacter ginsenosidimutans
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4236 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8UEH1|A0A5B8UEH1_9BACT Endolytic murein transglycosylase OS=Flavisolibacter ginsenosidimutans OX=661481 GN=mltG PE=3 SV=1
MM1 pKa = 7.27 YY2 pKa = 10.1 SYY4 pKa = 11.71 GLLEE8 pKa = 4.53 PGCYY12 pKa = 10.03 YY13 pKa = 10.87 LVQEE17 pKa = 4.42 EE18 pKa = 4.25 TDD20 pKa = 4.12 SIVEE24 pKa = 4.42 LIKK27 pKa = 10.41 VTVEE31 pKa = 4.01 TDD33 pKa = 2.23 HH34 pKa = 7.18 CMYY37 pKa = 10.08 VFKK40 pKa = 11.32 YY41 pKa = 10.34 GDD43 pKa = 3.5 EE44 pKa = 4.73 LVTEE48 pKa = 4.83 WRR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 7.24 TDD54 pKa = 3.95 AIFDD58 pKa = 4.27 IIEE61 pKa = 4.35 CLDD64 pKa = 3.74 DD65 pKa = 4.51 KK66 pKa = 11.31 KK67 pKa = 11.05 IEE69 pKa = 4.04 AWEE72 pKa = 3.79 KK73 pKa = 9.95 QYY75 pKa = 11.86 NNNEE79 pKa = 3.48 EE80 pKa = 4.17 SYY82 pKa = 10.65 YY83 pKa = 10.91 EE84 pKa = 4.08 EE85 pKa = 5.74 DD86 pKa = 3.6 EE87 pKa = 4.37 EE88 pKa = 4.83
Molecular weight: 10.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.906
IPC2_protein 4.088
IPC_protein 3.999
Toseland 3.834
ProMoST 4.075
Dawson 3.948
Bjellqvist 4.151
Wikipedia 3.821
Rodwell 3.834
Grimsley 3.745
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.19
Thurlkill 3.846
EMBOSS 3.834
Sillero 4.113
Patrickios 0.528
IPC_peptide 3.948
IPC2_peptide 4.101
IPC2.peptide.svr19 4.042
Protein with the highest isoelectric point:
>tr|A0A5B8ULW4|A0A5B8ULW4_9BACT DUF1211 domain-containing protein OS=Flavisolibacter ginsenosidimutans OX=661481 GN=FSB75_16565 PE=3 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPHH8 pKa = 4.58 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 6.91 TVHH16 pKa = 6.14 GFRR19 pKa = 11.84 KK20 pKa = 10.08 RR21 pKa = 11.84 MQSANGRR28 pKa = 11.84 KK29 pKa = 8.94 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 HH40 pKa = 5.59 KK41 pKa = 10.43 LTVSDD46 pKa = 3.83 EE47 pKa = 4.56 RR48 pKa = 11.84 KK49 pKa = 10.22 LKK51 pKa = 10.73
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.252
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4236
0
4236
1490540
32
2602
351.9
39.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.042 ± 0.036
0.905 ± 0.015
5.131 ± 0.025
5.704 ± 0.045
5.004 ± 0.027
7.013 ± 0.033
1.906 ± 0.018
6.033 ± 0.028
6.913 ± 0.035
9.442 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.018
5.359 ± 0.041
3.978 ± 0.024
3.858 ± 0.021
4.232 ± 0.026
6.325 ± 0.031
5.959 ± 0.047
6.814 ± 0.029
1.312 ± 0.015
3.937 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here