Atta cephalotes (Leafcutter ant)
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10634 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A158NYE1|A0A158NYE1_ATTCE Uncharacterized protein OS=Atta cephalotes OX=12957 GN=105625848 PE=3 SV=1
GG1 pKa = 7.27 SPLSRR6 pKa = 11.84 ADD8 pKa = 3.51 LRR10 pKa = 11.84 AVNVNLCDD18 pKa = 3.41 MCCILSAKK26 pKa = 9.88 VPSNDD31 pKa = 4.82 DD32 pKa = 2.99 PTLADD37 pKa = 4.05 KK38 pKa = 10.75 EE39 pKa = 4.6 AILASLNIKK48 pKa = 10.61 AMTFDD53 pKa = 3.55 DD54 pKa = 4.76 TIGVLSQDD62 pKa = 3.57 NEE64 pKa = 4.19 XGNLIAVGSPIVLQRR79 pKa = 11.84 RR80 pKa = 11.84 GSVYY84 pKa = 10.2 GANVPMITEE93 pKa = 4.79 LVNDD97 pKa = 4.27 SNVQFLDD104 pKa = 3.94 QDD106 pKa = 4.38 DD107 pKa = 5.59 DD108 pKa = 4.84 DD109 pKa = 6.57 DD110 pKa = 6.1 PDD112 pKa = 3.87 TEE114 pKa = 5.57 LYY116 pKa = 8.74 LTQPFACGTAFAVSVLDD133 pKa = 3.74 SLMSTTYY140 pKa = 10.43 FNQNALTLIRR150 pKa = 11.84 SLITGGATPEE160 pKa = 4.32 LEE162 pKa = 5.17 LILAEE167 pKa = 4.32 GAGLRR172 pKa = 11.84 GGYY175 pKa = 7.88 STADD179 pKa = 3.06 SLANRR184 pKa = 11.84 DD185 pKa = 3.44 RR186 pKa = 11.84 CRR188 pKa = 11.84 VGQIALYY195 pKa = 10.12 DD196 pKa = 3.97 GPLAQYY202 pKa = 11.29 GEE204 pKa = 4.26 GGKK207 pKa = 10.74 YY208 pKa = 10.55 GDD210 pKa = 4.82 LFVAALKK217 pKa = 10.61 SYY219 pKa = 10.77 GMLCIGLYY227 pKa = 10.17
Molecular weight: 24.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.986
IPC_protein 3.986
Toseland 3.757
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.368
Thurlkill 3.821
EMBOSS 3.948
Sillero 4.113
Patrickios 1.952
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A158NRM6|A0A158NRM6_ATTCE Dipeptidase OS=Atta cephalotes OX=12957 GN=105623397 PE=3 SV=1
MM1 pKa = 7.91 PPPPPPPSIVVFARR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 MASSSLSSSMPPPLSLPPPPPPPPPPPPPPPPPIGVIVFARR58 pKa = 11.84 HH59 pKa = 5.72 RR60 pKa = 11.84 LASSSSLSSMSSPPSPPNVVVFARR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 LTLSSSSSLL95 pKa = 3.58
Molecular weight: 9.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10483
151
10634
6079146
34
16023
571.7
64.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.957 ± 0.023
1.984 ± 0.034
5.467 ± 0.021
6.77 ± 0.041
3.579 ± 0.019
5.19 ± 0.035
2.52 ± 0.013
6.315 ± 0.023
6.541 ± 0.039
9.085 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.404 ± 0.013
5.308 ± 0.021
4.823 ± 0.028
4.357 ± 0.024
5.423 ± 0.02
8.111 ± 0.033
6.034 ± 0.021
5.832 ± 0.018
1.076 ± 0.007
3.21 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here