Congregibacter litoralis KT71
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3868 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4A7D8|A4A7D8_9GAMM Acetolactate synthase large subunit OS=Congregibacter litoralis KT71 OX=314285 GN=KT71_03132 PE=3 SV=1
MM1 pKa = 7.4 LTPGKK6 pKa = 9.31 VTDD9 pKa = 3.65 KK10 pKa = 11.05 ALWIAMLGMLTALACAAPRR29 pKa = 11.84 ADD31 pKa = 3.54 SLYY34 pKa = 10.39 VGAGAYY40 pKa = 7.66 FTDD43 pKa = 3.66 VEE45 pKa = 4.43 LSVGGEE51 pKa = 4.04 DD52 pKa = 5.66 DD53 pKa = 3.21 ITPAGFIGYY62 pKa = 8.97 QFLDD66 pKa = 3.81 SNVLMLSAEE75 pKa = 3.8 AGYY78 pKa = 10.97 YY79 pKa = 10.37 DD80 pKa = 4.21 LGAWSGTLDD89 pKa = 3.58 DD90 pKa = 3.98 VRR92 pKa = 11.84 YY93 pKa = 10.28 SVDD96 pKa = 3.25 ASALTVAGVAYY107 pKa = 10.55 VPLGPFFEE115 pKa = 5.22 VYY117 pKa = 10.66 AKK119 pKa = 10.75 AGFAAVEE126 pKa = 4.38 VKK128 pKa = 10.67 SRR130 pKa = 11.84 FADD133 pKa = 3.35 IPRR136 pKa = 11.84 TDD138 pKa = 4.82 DD139 pKa = 3.39 DD140 pKa = 4.18 TEE142 pKa = 4.22 TFIGAGVALDD152 pKa = 4.33 FFDD155 pKa = 4.0 TVDD158 pKa = 3.53 IYY160 pKa = 11.68 AEE162 pKa = 3.94 YY163 pKa = 10.66 LQFDD167 pKa = 3.8 NTINSQMVGVGIRR180 pKa = 11.84 FDD182 pKa = 3.77 FFF184 pKa = 5.35
Molecular weight: 19.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.49
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.126
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.846
Patrickios 0.998
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A4A3M6|A4A3M6_9GAMM Cytochrome c-type biogenesis protein OS=Congregibacter litoralis KT71 OX=314285 GN=KT71_16556 PE=3 SV=2
MM1 pKa = 7.68 KK2 pKa = 10.34 FPFWRR7 pKa = 11.84 RR8 pKa = 11.84 SLARR12 pKa = 11.84 TIFLGVAALGVLLWAASSQLGLGLNSLLAQFLVLMAALLLLIGLAAVVGIILGMLRR68 pKa = 11.84 RR69 pKa = 11.84 KK70 pKa = 9.64 RR71 pKa = 3.56
Molecular weight: 7.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3868
0
3868
1301601
29
4182
336.5
36.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.833 ± 0.046
0.97 ± 0.015
6.101 ± 0.037
6.377 ± 0.037
3.723 ± 0.024
7.94 ± 0.037
2.065 ± 0.019
4.935 ± 0.028
3.132 ± 0.03
10.763 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.018
3.026 ± 0.025
4.698 ± 0.029
3.65 ± 0.026
6.691 ± 0.035
6.476 ± 0.034
5.093 ± 0.041
7.123 ± 0.038
1.364 ± 0.014
2.638 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here