Pigeon adenovirus 1
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X5M219|X5M219_9ADEN Protein ORF62 OS=Pigeon adenovirus 1 OX=764030 GN=ORF62 PE=4 SV=1
MM1 pKa = 7.37 PQPSNVAVVAPKK13 pKa = 9.49 TKK15 pKa = 10.35 PEE17 pKa = 3.97 APRR20 pKa = 11.84 KK21 pKa = 8.71 RR22 pKa = 11.84 QLSAAALAAEE32 pKa = 4.25 QQQAEE37 pKa = 4.02 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 9.63 RR43 pKa = 11.84 LRR45 pKa = 11.84 RR46 pKa = 11.84 DD47 pKa = 3.03 ATDD50 pKa = 3.94 PDD52 pKa = 4.26 LNLVYY57 pKa = 9.81 PFWYY61 pKa = 8.98 TVEE64 pKa = 4.68 DD65 pKa = 3.62 PHH67 pKa = 8.45 VVNPPFLAPTGPLYY81 pKa = 10.78 DD82 pKa = 4.93 DD83 pKa = 4.55 NGNLNIRR90 pKa = 11.84 LTPPVTLVEE99 pKa = 4.82 DD100 pKa = 4.59 GVGLAYY106 pKa = 10.29 DD107 pKa = 3.55 QSLKK111 pKa = 10.98 VSDD114 pKa = 3.86 ATGAAAGVLGVQVDD128 pKa = 3.99 TEE130 pKa = 4.56 GPLTVTEE137 pKa = 5.51 DD138 pKa = 3.84 GLDD141 pKa = 3.78 LNTDD145 pKa = 3.34 DD146 pKa = 5.5 SLAVNDD152 pKa = 4.05 DD153 pKa = 3.12 WQLGVRR159 pKa = 11.84 FAEE162 pKa = 4.0 NQPFSVTTAGVSLLVDD178 pKa = 3.66 DD179 pKa = 4.98 TLLIAAADD187 pKa = 3.98 EE188 pKa = 4.3 EE189 pKa = 5.24 TPTSYY194 pKa = 11.23 EE195 pKa = 3.98 LGVHH199 pKa = 6.84 LNQNGPITADD209 pKa = 3.1 ADD211 pKa = 4.66 GIDD214 pKa = 4.89 LEE216 pKa = 4.79 VDD218 pKa = 3.21 NQTLQVTTNEE228 pKa = 3.85 QSQGVLAVKK237 pKa = 10.29 LKK239 pKa = 10.02 QQGGLSAGTSGIGINYY255 pKa = 9.32 DD256 pKa = 3.29 NEE258 pKa = 4.16 GALVVSDD265 pKa = 3.86 NTLQLKK271 pKa = 10.29 IGTDD275 pKa = 3.66 QPFSTTNGLSLNYY288 pKa = 9.78 DD289 pKa = 3.6 PNSFTVSGVTTSGTTTTMSQLAVKK313 pKa = 9.75 VASSNPLGKK322 pKa = 10.31 DD323 pKa = 3.03 NSGLKK328 pKa = 10.0 LVYY331 pKa = 9.06 NTQDD335 pKa = 3.17 FFDD338 pKa = 5.5 DD339 pKa = 3.81 NSSGLSAVTPIQYY352 pKa = 10.5 LSPYY356 pKa = 8.05 CTYY359 pKa = 10.95 SAGSPGLDD367 pKa = 2.94 TFDD370 pKa = 3.27 VTVRR374 pKa = 11.84 SSSGEE379 pKa = 3.66 NWTIAAYY386 pKa = 10.14 VVIANSGGICNGILYY401 pKa = 9.8 LHH403 pKa = 7.09 FDD405 pKa = 3.72 KK406 pKa = 11.14 KK407 pKa = 10.92 QLGTLGTGQGGEE419 pKa = 3.95 TGNYY423 pKa = 7.95 LRR425 pKa = 11.84 CAIVVNPSGNPGGNHH440 pKa = 6.48 SNFSNPRR447 pKa = 11.84 WWPEE451 pKa = 3.37 GSNSFLTPPGPPNAPPVNAVVSAIGRR477 pKa = 11.84 NNWYY481 pKa = 10.15 YY482 pKa = 9.84 GPPWRR487 pKa = 11.84 GVEE490 pKa = 4.11 VKK492 pKa = 10.59 LKK494 pKa = 10.62 SRR496 pKa = 11.84 STNTIGSTTTVTNTDD511 pKa = 2.66 AVAYY515 pKa = 6.92 YY516 pKa = 10.58 LPATAQGSNGNPNVIYY532 pKa = 9.68 FIYY535 pKa = 9.99 EE536 pKa = 4.13 VKK538 pKa = 10.44 FDD540 pKa = 3.9 WYY542 pKa = 11.35 NGTTNNGMGVQTSDD556 pKa = 5.04 PIPFSYY562 pKa = 10.36 LGSLPQFPRR571 pKa = 11.84 NSTGTISTTTTTTTTVITPALLPDD595 pKa = 3.99 VVPAPGPEE603 pKa = 4.5 PGTLPAPEE611 pKa = 5.38 AGEE614 pKa = 4.28 TQDD617 pKa = 3.41 TAEE620 pKa = 5.17 DD621 pKa = 4.51 LPHH624 pKa = 6.48 QPPEE628 pKa = 4.32 DD629 pKa = 3.49 PALEE633 pKa = 4.19 VEE635 pKa = 4.35 PDD637 pKa = 3.39 EE638 pKa = 5.87 DD639 pKa = 3.65 PALEE643 pKa = 4.22 VEE645 pKa = 4.46 APEE648 pKa = 4.52 DD649 pKa = 3.21 AAAEE653 pKa = 4.3 VPVVDD658 pKa = 5.07 DD659 pKa = 3.99 AALEE663 pKa = 4.11 EE664 pKa = 4.72 VPLEE668 pKa = 4.59 DD669 pKa = 4.54 PSEE672 pKa = 4.28 GSPAEE677 pKa = 4.29 DD678 pKa = 2.82 AALPEE683 pKa = 4.29 EE684 pKa = 4.83 DD685 pKa = 4.47 VAPEE689 pKa = 3.9 DD690 pKa = 3.64 TSVVDD695 pKa = 4.76 EE696 pKa = 5.29 LPPPPPQEE704 pKa = 4.28 PPAPDD709 pKa = 4.04 PEE711 pKa = 4.96 PPVTPPAEE719 pKa = 4.17 QPSLVIVDD727 pKa = 5.57 FVADD731 pKa = 4.28 PDD733 pKa = 4.55 PYY735 pKa = 11.15 LQFPQDD741 pKa = 3.62 HH742 pKa = 4.94 QHH744 pKa = 6.81 DD745 pKa = 4.56 RR746 pKa = 11.84 IILL749 pKa = 3.88
Molecular weight: 79.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.077
IPC2_protein 3.923
IPC_protein 3.948
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.643
Solomon 3.923
Lehninger 3.884
Nozaki 4.024
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.062
Patrickios 3.236
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.98
Protein with the highest isoelectric point:
>tr|X5LU16|X5LU16_9ADEN Hexon assembly protein 100K OS=Pigeon adenovirus 1 OX=764030 GN=100K PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.46 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 PRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGSTGRR31 pKa = 11.84 LVLRR35 pKa = 11.84 RR36 pKa = 11.84 LLGLGTTTSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 TRR53 pKa = 11.84 RR54 pKa = 11.84 VRR56 pKa = 11.84 GGAARR61 pKa = 11.84 RR62 pKa = 11.84 STTSTTRR69 pKa = 11.84 VIAVRR74 pKa = 11.84 TTRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 3.29
Molecular weight: 9.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.618
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.325
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.278
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
37
0
37
13690
37
1571
370.0
41.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.072 ± 0.509
1.819 ± 0.248
5.398 ± 0.282
5.778 ± 0.393
3.937 ± 0.253
6.275 ± 0.339
2.462 ± 0.253
2.476 ± 0.168
2.345 ± 0.185
9.905 ± 0.432
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.833 ± 0.152
3.492 ± 0.492
7.714 ± 0.458
3.901 ± 0.262
8.787 ± 0.666
6.45 ± 0.297
6.326 ± 0.664
6.896 ± 0.286
1.461 ± 0.153
3.674 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here