Sanxia tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGK5|A0A1L3KGK5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 4 OX=1923388 PE=4 SV=1
MM1 pKa = 7.74DD2 pKa = 4.29VKK4 pKa = 10.73PFEE7 pKa = 4.25KK8 pKa = 10.25TLDD11 pKa = 3.72NVVSGIKK18 pKa = 9.74EE19 pKa = 4.01RR20 pKa = 11.84IMYY23 pKa = 9.94IDD25 pKa = 3.96DD26 pKa = 4.02AGTRR30 pKa = 11.84RR31 pKa = 11.84PVCRR35 pKa = 11.84RR36 pKa = 11.84NAGEE40 pKa = 4.58LKK42 pKa = 10.73HH43 pKa = 6.41LTDD46 pKa = 4.62KK47 pKa = 11.3LADD50 pKa = 3.75CVPNPTRR57 pKa = 11.84MTRR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 4.14FIASRR67 pKa = 11.84NGRR70 pKa = 11.84LKK72 pKa = 10.52KK73 pKa = 10.49VYY75 pKa = 9.82EE76 pKa = 4.4RR77 pKa = 11.84ANEE80 pKa = 4.0MLDD83 pKa = 3.82DD84 pKa = 4.07QPSSLEE90 pKa = 3.89DD91 pKa = 3.39LARR94 pKa = 11.84TSLFTKK100 pKa = 9.6WEE102 pKa = 4.14RR103 pKa = 11.84SVHH106 pKa = 5.9KK107 pKa = 9.82PGKK110 pKa = 6.56TTVPRR115 pKa = 11.84IINPRR120 pKa = 11.84SPQFNILLGRR130 pKa = 11.84YY131 pKa = 7.03LTPIEE136 pKa = 4.03HH137 pKa = 6.67QIFEE141 pKa = 4.63GLQEE145 pKa = 4.25MLEE148 pKa = 4.31SPHH151 pKa = 6.38PVIAKK156 pKa = 10.17GLTQSEE162 pKa = 4.43KK163 pKa = 10.61GQIIADD169 pKa = 3.84IVNDD173 pKa = 4.82GYY175 pKa = 11.72VVVGLDD181 pKa = 3.28ASRR184 pKa = 11.84FDD186 pKa = 3.41QCISEE191 pKa = 4.54EE192 pKa = 4.05LLKK195 pKa = 10.24MEE197 pKa = 4.75HH198 pKa = 6.26NVYY201 pKa = 10.33LKK203 pKa = 10.53CYY205 pKa = 10.1QNDD208 pKa = 3.66RR209 pKa = 11.84LLRR212 pKa = 11.84ALLKK216 pKa = 10.75CQLDD220 pKa = 3.36NSGQYY225 pKa = 9.39IGRR228 pKa = 11.84DD229 pKa = 3.15GRR231 pKa = 11.84VKK233 pKa = 10.4VRR235 pKa = 11.84YY236 pKa = 8.79GAIRR240 pKa = 11.84CSGDD244 pKa = 3.03MNTSLGNCIISVLLSVLFCDD264 pKa = 4.17EE265 pKa = 4.24NGIGDD270 pKa = 3.87FRR272 pKa = 11.84VFCDD276 pKa = 4.84GDD278 pKa = 4.02DD279 pKa = 3.74LLLAVRR285 pKa = 11.84RR286 pKa = 11.84GDD288 pKa = 3.91LNKK291 pKa = 10.47LNPLQQWYY299 pKa = 8.46LQWGLRR305 pKa = 11.84MKK307 pKa = 10.59VEE309 pKa = 4.41EE310 pKa = 4.05PAYY313 pKa = 9.33TPEE316 pKa = 4.14AVEE319 pKa = 4.84FCQSRR324 pKa = 11.84PVCIDD329 pKa = 2.59GRR331 pKa = 11.84YY332 pKa = 9.67VLIRR336 pKa = 11.84DD337 pKa = 3.25VRR339 pKa = 11.84KK340 pKa = 10.17CINVDD345 pKa = 2.98YY346 pKa = 11.18SGFVNLQDD354 pKa = 3.38RR355 pKa = 11.84DD356 pKa = 3.78YY357 pKa = 9.72MLKK360 pKa = 9.51YY361 pKa = 9.9LRR363 pKa = 11.84AVGVCGSYY371 pKa = 10.34LAAGCPVLQAWYY383 pKa = 9.67GFGVRR388 pKa = 11.84VGTTGKK394 pKa = 10.53LDD396 pKa = 3.45VLDD399 pKa = 3.98TEE401 pKa = 5.26RR402 pKa = 11.84GFTRR406 pKa = 11.84QAKK409 pKa = 9.67LEE411 pKa = 4.03QRR413 pKa = 11.84SGAVMYY419 pKa = 10.49RR420 pKa = 11.84PIDD423 pKa = 4.08DD424 pKa = 3.97GARR427 pKa = 11.84EE428 pKa = 4.18SFRR431 pKa = 11.84LAFGIGAAEE440 pKa = 3.81QLLLEE445 pKa = 4.74SIFDD449 pKa = 3.6EE450 pKa = 4.38MTLEE454 pKa = 4.27RR455 pKa = 11.84GQLDD459 pKa = 3.47SHH461 pKa = 6.13PQEE464 pKa = 3.84ITDD467 pKa = 4.17FSNTFAFLVQPQHH480 pKa = 5.65GG481 pKa = 3.65

Molecular weight: 54.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGK5|A0A1L3KGK5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 4 OX=1923388 PE=4 SV=1
MM1 pKa = 7.26VKK3 pKa = 10.52LSNRR7 pKa = 11.84LVGKK11 pKa = 10.48LIDD14 pKa = 3.76RR15 pKa = 11.84ASGQRR20 pKa = 11.84PRR22 pKa = 11.84NGRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VKK29 pKa = 10.15RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84AAPRR36 pKa = 11.84GKK38 pKa = 9.54AATMRR43 pKa = 11.84RR44 pKa = 11.84EE45 pKa = 3.53IHH47 pKa = 6.13VPHH50 pKa = 6.89ANVLANPSTAPLHH63 pKa = 5.72GCVGIYY69 pKa = 10.0EE70 pKa = 4.47GEE72 pKa = 4.01QGNITRR78 pKa = 11.84LSLDD82 pKa = 3.32YY83 pKa = 10.7TIGGGAGNTCAYY95 pKa = 10.67YY96 pKa = 10.03IFHH99 pKa = 7.77PNTGLVSGAQTTAGSTAIVPAFGVGAGTSPGYY131 pKa = 10.77NLLNTNARR139 pKa = 11.84KK140 pKa = 9.88VRR142 pKa = 11.84GIAAGMRR149 pKa = 11.84FSIPSLSLTTIVGEE163 pKa = 4.32VCVGVCSADD172 pKa = 2.99TFMNVPNFTVDD183 pKa = 3.36QLFLHH188 pKa = 6.16SAGRR192 pKa = 11.84GPINKK197 pKa = 8.12ITQDD201 pKa = 3.77CAWYY205 pKa = 8.96PGSFDD210 pKa = 4.35ARR212 pKa = 11.84YY213 pKa = 8.1STYY216 pKa = 11.0AGTIADD222 pKa = 4.09TGSDD226 pKa = 3.4LSDD229 pKa = 3.14TNVFYY234 pKa = 10.15FAIRR238 pKa = 11.84GIPVSTNVQVKK249 pKa = 10.28LDD251 pKa = 3.98WVCEE255 pKa = 4.18WVAKK259 pKa = 10.57AGTGLVPSYY268 pKa = 10.39TSNPGSNHH276 pKa = 3.87QTTVAHH282 pKa = 6.18LHH284 pKa = 6.05EE285 pKa = 4.53NHH287 pKa = 7.01PGWFHH292 pKa = 5.57SVKK295 pKa = 10.62SEE297 pKa = 3.95AEE299 pKa = 3.92AVGKK303 pKa = 10.39NIVRR307 pKa = 11.84EE308 pKa = 4.19GVKK311 pKa = 10.04AARR314 pKa = 11.84HH315 pKa = 5.28KK316 pKa = 10.67AEE318 pKa = 3.75KK319 pKa = 9.14WVPTMLEE326 pKa = 3.67SGFAAFGLL334 pKa = 4.17

Molecular weight: 35.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1026

211

481

342.0

38.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.602 ± 1.394

2.339 ± 0.323

5.556 ± 1.078

5.653 ± 0.982

4.191 ± 0.413

9.064 ± 0.796

2.242 ± 0.471

4.971 ± 0.266

5.166 ± 0.927

7.895 ± 1.686

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.336

4.581 ± 0.436

3.996 ± 0.599

3.021 ± 1.046

7.7 ± 0.495

5.166 ± 0.749

5.556 ± 0.908

8.382 ± 0.711

1.17 ± 0.191

3.509 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski