Ruminococcus sp. AF31-8BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4089 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373NMV5|A0A373NMV5_9FIRM Hydrogenase OS=Ruminococcus sp. AF31-8BH OX=2293174 GN=DWZ38_06675 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.1KK3 pKa = 9.85SKK5 pKa = 10.82AVISLLTASAMTVGLMSTGTVAFAAEE31 pKa = 4.61EE32 pKa = 4.33STSDD36 pKa = 3.7LNVMLEE42 pKa = 4.33TPVQSLDD49 pKa = 3.42PQQATDD55 pKa = 3.57GTSFEE60 pKa = 4.7VIADD64 pKa = 3.82YY65 pKa = 11.2TDD67 pKa = 4.31GLMQMDD73 pKa = 4.45SDD75 pKa = 4.26GQAVPAIAEE84 pKa = 4.38SYY86 pKa = 10.74DD87 pKa = 3.68LSDD90 pKa = 5.66DD91 pKa = 3.51GLTYY95 pKa = 10.41TFHH98 pKa = 7.78LRR100 pKa = 11.84DD101 pKa = 3.67DD102 pKa = 4.53AQWSNGDD109 pKa = 3.83PVTASDD115 pKa = 6.33FVFGWQRR122 pKa = 11.84AVDD125 pKa = 3.68PDD127 pKa = 3.78VASEE131 pKa = 3.99YY132 pKa = 10.9AYY134 pKa = 9.8MLSDD138 pKa = 3.33IGQVEE143 pKa = 4.06NAAEE147 pKa = 4.3IIAGEE152 pKa = 4.1KK153 pKa = 10.32DD154 pKa = 3.25KK155 pKa = 11.97SEE157 pKa = 4.61LGVTAVDD164 pKa = 4.36DD165 pKa = 3.65KK166 pKa = 11.29TLEE169 pKa = 4.09VKK171 pKa = 10.94LNVPVSYY178 pKa = 10.0FLSLMYY184 pKa = 10.48FPTFYY189 pKa = 10.31PVNEE193 pKa = 4.38AFFEE197 pKa = 4.54SCADD201 pKa = 3.58TFGTSPEE208 pKa = 4.23TTLSNGAFVLDD219 pKa = 4.21TYY221 pKa = 11.16EE222 pKa = 4.03PAATEE227 pKa = 3.46IHH229 pKa = 5.7LTKK232 pKa = 10.83NADD235 pKa = 3.43YY236 pKa = 11.1YY237 pKa = 11.44DD238 pKa = 4.51ADD240 pKa = 4.42NISLPGLNYY249 pKa = 10.15QVIQDD254 pKa = 3.85SQQALMSYY262 pKa = 8.01QTGDD266 pKa = 3.38LDD268 pKa = 3.73LTLVNGEE275 pKa = 4.11QVDD278 pKa = 3.99QVEE281 pKa = 4.76DD282 pKa = 3.93DD283 pKa = 4.35PEE285 pKa = 4.45FMTIGAGYY293 pKa = 9.66LWYY296 pKa = 10.58VSPNISAVPEE306 pKa = 3.96LANLNIRR313 pKa = 11.84LAMTMAIDD321 pKa = 3.95RR322 pKa = 11.84EE323 pKa = 4.59SITTDD328 pKa = 3.12VLKK331 pKa = 10.97DD332 pKa = 3.64GSLPTYY338 pKa = 9.0TAVPMQFAAGPDD350 pKa = 3.54GSDD353 pKa = 3.46FSEE356 pKa = 4.48DD357 pKa = 3.31QEE359 pKa = 4.58KK360 pKa = 10.76FSDD363 pKa = 3.61VCTYY367 pKa = 10.76DD368 pKa = 3.07AEE370 pKa = 4.33KK371 pKa = 10.75AADD374 pKa = 3.58YY375 pKa = 7.66WAKK378 pKa = 10.5GLEE381 pKa = 4.18EE382 pKa = 4.42LGEE385 pKa = 4.4TEE387 pKa = 4.75VEE389 pKa = 3.47LDD391 pKa = 4.0MIVDD395 pKa = 4.57ADD397 pKa = 4.02DD398 pKa = 5.05APQKK402 pKa = 9.76VAQVLKK408 pKa = 9.3EE409 pKa = 3.88QWEE412 pKa = 4.24TALPGLTVNLTVEE425 pKa = 4.27PKK427 pKa = 9.36KK428 pKa = 10.91QRR430 pKa = 11.84VEE432 pKa = 4.08DD433 pKa = 4.04MQNGTYY439 pKa = 9.88EE440 pKa = 4.1VALTRR445 pKa = 11.84WGPDD449 pKa = 2.99YY450 pKa = 10.98ADD452 pKa = 3.44PMTYY456 pKa = 10.43LGMWITDD463 pKa = 3.18NSNNYY468 pKa = 9.1GLWSSEE474 pKa = 4.1EE475 pKa = 3.93YY476 pKa = 10.84DD477 pKa = 4.72SIIAEE482 pKa = 4.44CTTGDD487 pKa = 3.74LCTDD491 pKa = 3.57AEE493 pKa = 4.58GRR495 pKa = 11.84WARR498 pKa = 11.84MYY500 pKa = 10.72DD501 pKa = 3.46AEE503 pKa = 4.74KK504 pKa = 10.87LVMDD508 pKa = 5.21DD509 pKa = 4.86AVIFPLYY516 pKa = 8.56TQANAEE522 pKa = 4.2MMSSSVTGVDD532 pKa = 4.28FHH534 pKa = 6.99PCALNRR540 pKa = 11.84VYY542 pKa = 11.08KK543 pKa = 10.74NAVKK547 pKa = 10.66SEE549 pKa = 3.83

Molecular weight: 60.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373NU95|A0A373NU95_9FIRM Peptidyl-tRNA hydrolase OS=Ruminococcus sp. AF31-8BH OX=2293174 GN=pth PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4089

0

4089

1301907

24

3759

318.4

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.417 ± 0.043

1.555 ± 0.016

5.629 ± 0.035

7.691 ± 0.045

4.113 ± 0.028

7.031 ± 0.04

1.784 ± 0.018

7.037 ± 0.031

6.949 ± 0.036

8.936 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.154 ± 0.02

4.416 ± 0.028

3.308 ± 0.026

3.507 ± 0.023

4.325 ± 0.034

5.785 ± 0.033

5.412 ± 0.034

6.73 ± 0.03

0.998 ± 0.012

4.223 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski