Amino acid dipepetide frequency for Ruminococcus sp. AF31-8BH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.351AlaAla: 7.351 ± 0.101
1.137AlaCys: 1.137 ± 0.033
4.758AlaAsp: 4.758 ± 0.069
5.361AlaGlu: 5.361 ± 0.077
3.061AlaPhe: 3.061 ± 0.049
5.926AlaGly: 5.926 ± 0.081
1.161AlaHis: 1.161 ± 0.033
4.682AlaIle: 4.682 ± 0.067
4.804AlaLys: 4.804 ± 0.074
6.682AlaLeu: 6.682 ± 0.078
2.35AlaMet: 2.35 ± 0.046
2.59AlaAsn: 2.59 ± 0.05
2.041AlaPro: 2.041 ± 0.046
2.508AlaGln: 2.508 ± 0.049
3.007AlaArg: 3.007 ± 0.047
4.043AlaSer: 4.043 ± 0.069
3.123AlaThr: 3.123 ± 0.055
6.18AlaVal: 6.18 ± 0.075
0.639AlaTrp: 0.639 ± 0.024
2.77AlaTyr: 2.77 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.028
0.291CysCys: 0.291 ± 0.016
0.849CysAsp: 0.849 ± 0.027
0.958CysGlu: 0.958 ± 0.028
0.674CysPhe: 0.674 ± 0.025
1.541CysGly: 1.541 ± 0.044
0.325CysHis: 0.325 ± 0.018
1.12CysIle: 1.12 ± 0.029
0.887CysLys: 0.887 ± 0.026
1.244CysLeu: 1.244 ± 0.031
0.495CysMet: 0.495 ± 0.02
0.587CysAsn: 0.587 ± 0.026
0.669CysPro: 0.669 ± 0.03
0.551CysGln: 0.551 ± 0.028
0.76CysArg: 0.76 ± 0.025
0.94CysSer: 0.94 ± 0.026
0.804CysThr: 0.804 ± 0.03
1.026CysVal: 1.026 ± 0.028
0.15CysTrp: 0.15 ± 0.012
0.611CysTyr: 0.611 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.953AspAla: 3.953 ± 0.06
0.831AspCys: 0.831 ± 0.029
2.806AspAsp: 2.806 ± 0.066
4.676AspGlu: 4.676 ± 0.064
2.817AspPhe: 2.817 ± 0.047
4.52AspGly: 4.52 ± 0.078
0.924AspHis: 0.924 ± 0.03
4.285AspIle: 4.285 ± 0.054
3.44AspLys: 3.44 ± 0.055
4.582AspLeu: 4.582 ± 0.063
1.904AspMet: 1.904 ± 0.042
2.249AspAsn: 2.249 ± 0.04
1.86AspPro: 1.86 ± 0.037
1.628AspGln: 1.628 ± 0.039
2.261AspArg: 2.261 ± 0.045
3.184AspSer: 3.184 ± 0.054
3.137AspThr: 3.137 ± 0.059
3.785AspVal: 3.785 ± 0.057
0.626AspTrp: 0.626 ± 0.024
2.821AspTyr: 2.821 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.607GluAla: 5.607 ± 0.065
0.886GluCys: 0.886 ± 0.03
4.386GluAsp: 4.386 ± 0.068
7.38GluGlu: 7.38 ± 0.106
2.67GluPhe: 2.67 ± 0.048
4.331GluGly: 4.331 ± 0.069
1.408GluHis: 1.408 ± 0.032
5.631GluIle: 5.631 ± 0.079
7.375GluLys: 7.375 ± 0.095
6.667GluLeu: 6.667 ± 0.072
2.476GluMet: 2.476 ± 0.046
4.727GluAsn: 4.727 ± 0.065
1.886GluPro: 1.886 ± 0.042
2.915GluGln: 2.915 ± 0.058
3.197GluArg: 3.197 ± 0.055
3.3GluSer: 3.3 ± 0.056
3.801GluThr: 3.801 ± 0.058
4.332GluVal: 4.332 ± 0.066
0.735GluTrp: 0.735 ± 0.029
3.199GluTyr: 3.199 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.968PheAla: 2.968 ± 0.045
0.838PheCys: 0.838 ± 0.026
2.443PheAsp: 2.443 ± 0.045
2.576PheGlu: 2.576 ± 0.046
1.886PhePhe: 1.886 ± 0.043
3.092PheGly: 3.092 ± 0.054
0.935PheHis: 0.935 ± 0.025
2.602PheIle: 2.602 ± 0.051
1.9PheLys: 1.9 ± 0.042
4.086PheLeu: 4.086 ± 0.069
1.188PheMet: 1.188 ± 0.033
1.498PheAsn: 1.498 ± 0.037
1.442PhePro: 1.442 ± 0.033
1.527PheGln: 1.527 ± 0.036
1.71PheArg: 1.71 ± 0.034
2.963PheSer: 2.963 ± 0.061
2.446PheThr: 2.446 ± 0.048
2.707PheVal: 2.707 ± 0.048
0.477PheTrp: 0.477 ± 0.022
1.85PheTyr: 1.85 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
4.889GlyAla: 4.889 ± 0.086
1.267GlyCys: 1.267 ± 0.033
3.412GlyAsp: 3.412 ± 0.055
4.884GlyGlu: 4.884 ± 0.066
3.079GlyPhe: 3.079 ± 0.05
4.576GlyGly: 4.576 ± 0.09
1.255GlyHis: 1.255 ± 0.031
6.073GlyIle: 6.073 ± 0.084
5.78GlyLys: 5.78 ± 0.079
5.56GlyLeu: 5.56 ± 0.083
2.518GlyMet: 2.518 ± 0.045
3.353GlyAsn: 3.353 ± 0.059
1.202GlyPro: 1.202 ± 0.031
2.174GlyGln: 2.174 ± 0.047
2.956GlyArg: 2.956 ± 0.056
4.049GlySer: 4.049 ± 0.06
4.356GlyThr: 4.356 ± 0.067
4.796GlyVal: 4.796 ± 0.067
0.75GlyTrp: 0.75 ± 0.029
3.379GlyTyr: 3.379 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.131HisAla: 1.131 ± 0.036
0.336HisCys: 0.336 ± 0.017
0.898HisAsp: 0.898 ± 0.03
1.144HisGlu: 1.144 ± 0.032
0.879HisPhe: 0.879 ± 0.027
1.299HisGly: 1.299 ± 0.031
0.436HisHis: 0.436 ± 0.027
1.374HisIle: 1.374 ± 0.032
1.058HisLys: 1.058 ± 0.032
1.496HisLeu: 1.496 ± 0.035
0.564HisMet: 0.564 ± 0.026
0.783HisAsn: 0.783 ± 0.023
0.898HisPro: 0.898 ± 0.026
0.587HisGln: 0.587 ± 0.022
0.811HisArg: 0.811 ± 0.025
0.99HisSer: 0.99 ± 0.029
1.015HisThr: 1.015 ± 0.031
1.139HisVal: 1.139 ± 0.033
0.187HisTrp: 0.187 ± 0.014
0.814HisTyr: 0.814 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.313IleAla: 5.313 ± 0.073
1.355IleCys: 1.355 ± 0.031
3.664IleAsp: 3.664 ± 0.054
4.314IleGlu: 4.314 ± 0.069
3.017IlePhe: 3.017 ± 0.056
4.788IleGly: 4.788 ± 0.076
1.433IleHis: 1.433 ± 0.033
4.481IleIle: 4.481 ± 0.082
3.814IleLys: 3.814 ± 0.057
7.144IleLeu: 7.144 ± 0.092
1.953IleMet: 1.953 ± 0.047
2.723IleAsn: 2.723 ± 0.047
3.107IlePro: 3.107 ± 0.052
2.631IleGln: 2.631 ± 0.043
3.597IleArg: 3.597 ± 0.055
4.937IleSer: 4.937 ± 0.069
4.062IleThr: 4.062 ± 0.053
4.546IleVal: 4.546 ± 0.06
0.691IleTrp: 0.691 ± 0.024
2.796IleTyr: 2.796 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
5.318LysAla: 5.318 ± 0.07
0.836LysCys: 0.836 ± 0.03
3.93LysAsp: 3.93 ± 0.055
6.918LysGlu: 6.918 ± 0.09
1.959LysPhe: 1.959 ± 0.045
4.092LysGly: 4.092 ± 0.059
1.005LysHis: 1.005 ± 0.03
4.791LysIle: 4.791 ± 0.068
6.507LysLys: 6.507 ± 0.074
5.444LysLeu: 5.444 ± 0.066
2.396LysMet: 2.396 ± 0.044
3.718LysAsn: 3.718 ± 0.056
1.976LysPro: 1.976 ± 0.043
2.477LysGln: 2.477 ± 0.048
3.19LysArg: 3.19 ± 0.056
3.377LysSer: 3.377 ± 0.049
3.687LysThr: 3.687 ± 0.061
4.275LysVal: 4.275 ± 0.063
0.694LysTrp: 0.694 ± 0.023
2.895LysTyr: 2.895 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
6.66LeuAla: 6.66 ± 0.071
1.531LeuCys: 1.531 ± 0.035
5.245LeuAsp: 5.245 ± 0.06
6.536LeuGlu: 6.536 ± 0.095
3.705LeuPhe: 3.705 ± 0.067
5.991LeuGly: 5.991 ± 0.074
1.61LeuHis: 1.61 ± 0.037
5.692LeuIle: 5.692 ± 0.087
6.105LeuLys: 6.105 ± 0.078
8.604LeuLeu: 8.604 ± 0.131
2.687LeuMet: 2.687 ± 0.05
3.833LeuAsn: 3.833 ± 0.052
3.503LeuPro: 3.503 ± 0.058
3.05LeuGln: 3.05 ± 0.052
3.685LeuArg: 3.685 ± 0.059
6.096LeuSer: 6.096 ± 0.078
5.049LeuThr: 5.049 ± 0.065
5.551LeuVal: 5.551 ± 0.075
0.81LeuTrp: 0.81 ± 0.027
3.416LeuTyr: 3.416 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.473MetAla: 2.473 ± 0.044
0.391MetCys: 0.391 ± 0.016
1.975MetAsp: 1.975 ± 0.041
2.828MetGlu: 2.828 ± 0.048
0.989MetPhe: 0.989 ± 0.03
2.134MetGly: 2.134 ± 0.045
0.396MetHis: 0.396 ± 0.018
2.147MetIle: 2.147 ± 0.044
2.669MetLys: 2.669 ± 0.036
2.728MetLeu: 2.728 ± 0.047
0.941MetMet: 0.941 ± 0.03
1.661MetAsn: 1.661 ± 0.04
1.158MetPro: 1.158 ± 0.031
1.078MetGln: 1.078 ± 0.029
1.284MetArg: 1.284 ± 0.031
1.738MetSer: 1.738 ± 0.04
1.763MetThr: 1.763 ± 0.042
2.001MetVal: 2.001 ± 0.04
0.234MetTrp: 0.234 ± 0.014
0.949MetTyr: 0.949 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.218AsnAla: 3.218 ± 0.048
0.69AsnCys: 0.69 ± 0.024
2.104AsnAsp: 2.104 ± 0.04
2.829AsnGlu: 2.829 ± 0.038
1.65AsnPhe: 1.65 ± 0.035
3.707AsnGly: 3.707 ± 0.054
0.827AsnHis: 0.827 ± 0.027
3.282AsnIle: 3.282 ± 0.055
2.552AsnLys: 2.552 ± 0.043
4.053AsnLeu: 4.053 ± 0.062
1.448AsnMet: 1.448 ± 0.035
1.763AsnAsn: 1.763 ± 0.042
2.125AsnPro: 2.125 ± 0.04
1.668AsnGln: 1.668 ± 0.035
2.055AsnArg: 2.055 ± 0.039
2.476AsnSer: 2.476 ± 0.05
2.466AsnThr: 2.466 ± 0.044
2.833AsnVal: 2.833 ± 0.045
0.476AsnTrp: 0.476 ± 0.018
1.94AsnTyr: 1.94 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.425ProAla: 2.425 ± 0.047
0.451ProCys: 0.451 ± 0.018
2.411ProAsp: 2.411 ± 0.047
3.371ProGlu: 3.371 ± 0.057
1.538ProPhe: 1.538 ± 0.037
2.39ProGly: 2.39 ± 0.05
0.563ProHis: 0.563 ± 0.022
1.951ProIle: 1.951 ± 0.04
1.923ProLys: 1.923 ± 0.044
2.769ProLeu: 2.769 ± 0.046
0.907ProMet: 0.907 ± 0.026
1.208ProAsn: 1.208 ± 0.032
0.74ProPro: 0.74 ± 0.025
1.111ProGln: 1.111 ± 0.03
1.024ProArg: 1.024 ± 0.028
1.767ProSer: 1.767 ± 0.037
1.608ProThr: 1.608 ± 0.048
3.046ProVal: 3.046 ± 0.051
0.336ProTrp: 0.336 ± 0.017
1.537ProTyr: 1.537 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.666GlnAla: 2.666 ± 0.048
0.383GlnCys: 0.383 ± 0.018
1.804GlnAsp: 1.804 ± 0.037
3.208GlnGlu: 3.208 ± 0.063
1.171GlnPhe: 1.171 ± 0.03
2.049GlnGly: 2.049 ± 0.048
0.486GlnHis: 0.486 ± 0.019
2.742GlnIle: 2.742 ± 0.046
3.031GlnLys: 3.031 ± 0.057
3.108GlnLeu: 3.108 ± 0.051
1.267GlnMet: 1.267 ± 0.035
1.837GlnAsn: 1.837 ± 0.041
1.038GlnPro: 1.038 ± 0.033
1.316GlnGln: 1.316 ± 0.035
1.473GlnArg: 1.473 ± 0.035
1.745GlnSer: 1.745 ± 0.041
1.84GlnThr: 1.84 ± 0.041
2.145GlnVal: 2.145 ± 0.039
0.339GlnTrp: 0.339 ± 0.017
1.426GlnTyr: 1.426 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.612ArgAla: 2.612 ± 0.047
0.605ArgCys: 0.605 ± 0.019
2.126ArgAsp: 2.126 ± 0.047
3.708ArgGlu: 3.708 ± 0.063
1.808ArgPhe: 1.808 ± 0.044
2.323ArgGly: 2.323 ± 0.047
0.846ArgHis: 0.846 ± 0.026
3.433ArgIle: 3.433 ± 0.056
3.652ArgLys: 3.652 ± 0.057
3.854ArgLeu: 3.854 ± 0.06
1.557ArgMet: 1.557 ± 0.036
2.17ArgAsn: 2.17 ± 0.043
1.261ArgPro: 1.261 ± 0.034
1.837ArgGln: 1.837 ± 0.04
2.17ArgArg: 2.17 ± 0.047
2.175ArgSer: 2.175 ± 0.042
2.32ArgThr: 2.32 ± 0.043
2.447ArgVal: 2.447 ± 0.043
0.432ArgTrp: 0.432 ± 0.021
1.913ArgTyr: 1.913 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
4.134SerAla: 4.134 ± 0.059
0.874SerCys: 0.874 ± 0.027
3.293SerAsp: 3.293 ± 0.055
3.917SerGlu: 3.917 ± 0.055
2.706SerPhe: 2.706 ± 0.05
4.947SerGly: 4.947 ± 0.079
1.131SerHis: 1.131 ± 0.027
3.864SerIle: 3.864 ± 0.068
3.263SerLys: 3.263 ± 0.051
5.053SerLeu: 5.053 ± 0.065
1.861SerMet: 1.861 ± 0.037
2.151SerAsn: 2.151 ± 0.043
1.747SerPro: 1.747 ± 0.036
2.139SerGln: 2.139 ± 0.048
2.773SerArg: 2.773 ± 0.056
3.587SerSer: 3.587 ± 0.072
2.832SerThr: 2.832 ± 0.055
4.294SerVal: 4.294 ± 0.059
0.673SerTrp: 0.673 ± 0.025
2.607SerTyr: 2.607 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
4.458ThrAla: 4.458 ± 0.072
0.748ThrCys: 0.748 ± 0.035
3.427ThrAsp: 3.427 ± 0.062
3.844ThrGlu: 3.844 ± 0.061
2.224ThrPhe: 2.224 ± 0.045
4.603ThrGly: 4.603 ± 0.073
0.858ThrHis: 0.858 ± 0.026
3.793ThrIle: 3.793 ± 0.06
3.003ThrLys: 3.003 ± 0.043
4.775ThrLeu: 4.775 ± 0.059
1.469ThrMet: 1.469 ± 0.032
1.987ThrAsn: 1.987 ± 0.039
2.177ThrPro: 2.177 ± 0.053
1.609ThrGln: 1.609 ± 0.038
2.123ThrArg: 2.123 ± 0.042
3.094ThrSer: 3.094 ± 0.054
2.825ThrThr: 2.825 ± 0.063
4.302ThrVal: 4.302 ± 0.061
0.561ThrTrp: 0.561 ± 0.022
2.235ThrTyr: 2.235 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
4.53ValAla: 4.53 ± 0.07
1.191ValCys: 1.191 ± 0.032
3.652ValAsp: 3.652 ± 0.056
4.583ValGlu: 4.583 ± 0.074
3.004ValPhe: 3.004 ± 0.047
4.19ValGly: 4.19 ± 0.066
1.113ValHis: 1.113 ± 0.03
4.936ValIle: 4.936 ± 0.064
4.569ValLys: 4.569 ± 0.063
6.676ValLeu: 6.676 ± 0.08
2.039ValMet: 2.039 ± 0.042
2.847ValAsn: 2.847 ± 0.046
2.524ValPro: 2.524 ± 0.045
2.196ValGln: 2.196 ± 0.039
2.714ValArg: 2.714 ± 0.048
4.398ValSer: 4.398 ± 0.059
4.126ValThr: 4.126 ± 0.065
4.589ValVal: 4.589 ± 0.076
0.671ValTrp: 0.671 ± 0.023
2.753ValTyr: 2.753 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.594TrpAla: 0.594 ± 0.019
0.172TrpCys: 0.172 ± 0.012
0.568TrpAsp: 0.568 ± 0.02
0.793TrpGlu: 0.793 ± 0.026
0.412TrpPhe: 0.412 ± 0.018
0.687TrpGly: 0.687 ± 0.023
0.212TrpHis: 0.212 ± 0.014
0.694TrpIle: 0.694 ± 0.022
0.864TrpLys: 0.864 ± 0.03
0.974TrpLeu: 0.974 ± 0.027
0.34TrpMet: 0.34 ± 0.017
0.621TrpAsn: 0.621 ± 0.023
0.215TrpPro: 0.215 ± 0.013
0.379TrpGln: 0.379 ± 0.018
0.383TrpArg: 0.383 ± 0.016
0.534TrpSer: 0.534 ± 0.02
0.492TrpThr: 0.492 ± 0.023
0.525TrpVal: 0.525 ± 0.02
0.113TrpTrp: 0.113 ± 0.011
0.415TrpTyr: 0.415 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.804TyrAla: 2.804 ± 0.053
0.738TyrCys: 0.738 ± 0.026
2.542TyrAsp: 2.542 ± 0.049
3.08TyrGlu: 3.08 ± 0.049
1.884TyrPhe: 1.884 ± 0.049
3.122TyrGly: 3.122 ± 0.053
0.916TyrHis: 0.916 ± 0.03
2.792TyrIle: 2.792 ± 0.051
2.29TyrLys: 2.29 ± 0.04
3.857TyrLeu: 3.857 ± 0.059
1.179TyrMet: 1.179 ± 0.032
1.857TyrAsn: 1.857 ± 0.036
1.504TyrPro: 1.504 ± 0.035
1.694TyrGln: 1.694 ± 0.04
2.078TyrArg: 2.078 ± 0.038
2.455TyrSer: 2.455 ± 0.049
2.366TyrThr: 2.366 ± 0.048
2.781TyrVal: 2.781 ± 0.044
0.389TyrTrp: 0.389 ± 0.022
1.903TyrTyr: 1.903 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4089 proteins (1301907 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski