Fusarium vanettenii (strain ATCC MYA-4622 / CBS 123669 / FGSC 9596 / NRRL 45880 / 77-13-4) (Fusarium solani subsp. pisi)
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7YQ68|C7YQ68_FUSV7 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 OS=Fusarium vanettenii (strain ATCC MYA-4622 / CBS 123669 / FGSC 9596 / NRRL 45880 / 77-13-4) OX=660122 GN=NECHADRAFT_78998 PE=3 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 VLLSRR7 pKa = 11.84 LIALAIFLPLGVGFTIVTNSDD28 pKa = 3.13 GNTLGNAMMPGSGITLVSGTYY49 pKa = 9.28 TGASVAAGTFSDD61 pKa = 4.6 GPFGISSGTILTSGRR76 pKa = 11.84 ADD78 pKa = 3.4 GATVAGSDD86 pKa = 4.12 NDD88 pKa = 3.78 NGQPGYY94 pKa = 10.14 MGGNTFDD101 pKa = 4.03 AAVLSLDD108 pKa = 3.41 ITIAPPFTGFEE119 pKa = 4.32 LEE121 pKa = 4.66 FAFASNDD128 pKa = 3.38 YY129 pKa = 10.93 GGQQQFDD136 pKa = 4.38 DD137 pKa = 4.17 EE138 pKa = 4.83 AADD141 pKa = 3.58 NTRR144 pKa = 11.84 TIFDD148 pKa = 4.14 LVNTFMDD155 pKa = 4.3 PPYY158 pKa = 10.57 SINPPNSLTAYY169 pKa = 9.26 DD170 pKa = 4.01 KK171 pKa = 11.2 SSPPVLIGFATGSGAFNVKK190 pKa = 9.76 IAVYY194 pKa = 10.01 DD195 pKa = 4.06 LNDD198 pKa = 3.64 GFEE201 pKa = 4.71 DD202 pKa = 3.51 SAALIRR208 pKa = 11.84 MRR210 pKa = 11.84 GCEE213 pKa = 3.82 NCAAGPVINYY223 pKa = 5.8 EE224 pKa = 4.35 TKK226 pKa = 10.03 TVTVGPGQAEE236 pKa = 4.18 YY237 pKa = 10.67 FSTIKK242 pKa = 10.88 ASGTATGYY250 pKa = 9.14 YY251 pKa = 9.24 EE252 pKa = 4.57 KK253 pKa = 10.85 GVAATTTADD262 pKa = 3.21 TTTTEE267 pKa = 4.18 EE268 pKa = 4.32 TTTTAEE274 pKa = 4.15 TTTTAEE280 pKa = 4.1 TTTTEE285 pKa = 4.05 EE286 pKa = 4.16 TTTAEE291 pKa = 4.14 TTTTEE296 pKa = 4.11 EE297 pKa = 4.29 TTTTAEE303 pKa = 4.1 TTTEE307 pKa = 4.1 FTTEE311 pKa = 3.49 TTTYY315 pKa = 7.93 FTTEE319 pKa = 3.68 TTTDD323 pKa = 3.73 FTTEE327 pKa = 3.74 TTTDD331 pKa = 3.73 FTTEE335 pKa = 3.74 TTTDD339 pKa = 3.73 FTTEE343 pKa = 3.41 TTTYY347 pKa = 9.14 VTTDD351 pKa = 3.11 TTTVPDD357 pKa = 3.79 TTTTMDD363 pKa = 3.25 TTTTMDD369 pKa = 3.16 TTTAIDD375 pKa = 3.83 TTTTTYY381 pKa = 7.38 TTTAEE386 pKa = 4.43 DD387 pKa = 3.65 STTTADD393 pKa = 3.36 STTTTDD399 pKa = 5.35 LEE401 pKa = 4.65 TTTDD405 pKa = 3.24 STTTTDD411 pKa = 2.53 ITTTADD417 pKa = 3.2 TTVPTDD423 pKa = 3.29 STAVIDD429 pKa = 3.72 STTIIDD435 pKa = 3.79 STTTTNPTEE444 pKa = 4.41 DD445 pKa = 4.12 LSTSIDD451 pKa = 3.47 STTTFQSSEE460 pKa = 3.96 NTQGSTTDD468 pKa = 3.93 DD469 pKa = 4.04 GEE471 pKa = 4.79 STTTTSDD478 pKa = 3.43 LATTDD483 pKa = 3.12 TTEE486 pKa = 4.08 NLGEE490 pKa = 4.29 TTSTAITTSAQIPSIASTEE509 pKa = 4.17 SDD511 pKa = 3.35 TTTAILSTLEE521 pKa = 3.92 SSSALEE527 pKa = 4.06 ATPTSQSMTEE537 pKa = 4.01 TTPDD541 pKa = 3.32 STSDD545 pKa = 3.01 ISTLEE550 pKa = 4.08 SNEE553 pKa = 4.43 PEE555 pKa = 4.3 PQPSTASEE563 pKa = 4.52 LFTSSVDD570 pKa = 3.48 TPTGSVEE577 pKa = 4.13 PPSTNTPVGQSSTSALLPEE596 pKa = 4.65 TLSPDD601 pKa = 3.12 TSSISFPLPEE611 pKa = 4.38 SLSTGSDD618 pKa = 3.08 SQAPLSTTLSTQDD631 pKa = 3.36 SVTVDD636 pKa = 3.62 LTASSSPLATSISADD651 pKa = 3.37 SSILSSPDD659 pKa = 3.03 STSTLSGSTPNASNAPTIEE678 pKa = 3.9 RR679 pKa = 11.84 YY680 pKa = 10.28 VYY682 pKa = 9.78 FGCLSSVQGYY692 pKa = 9.1 PSFNIVGSATDD703 pKa = 3.7 MTPQKK708 pKa = 10.22 CISLAAGRR716 pKa = 11.84 RR717 pKa = 11.84 FVGVHH722 pKa = 5.48 LTSCYY727 pKa = 10.83 ASDD730 pKa = 3.83 SLVSSTLTQDD740 pKa = 3.74 ASCDD744 pKa = 3.94 LPCPGDD750 pKa = 3.41 PTLFCGGFLNADD762 pKa = 3.09 GGMSKK767 pKa = 9.31 RR768 pKa = 11.84 HH769 pKa = 5.02 RR770 pKa = 11.84 LEE772 pKa = 4.19 RR773 pKa = 11.84 RR774 pKa = 11.84 ISPRR778 pKa = 11.84 DD779 pKa = 3.59 APPGILLTLYY789 pKa = 10.52 LFADD793 pKa = 5.24 AIIEE797 pKa = 4.23 SSSEE801 pKa = 3.47 IAAVSTSSTSPAQTVASQSNSVDD824 pKa = 3.77 FSISPTLSEE833 pKa = 4.21 PVRR836 pKa = 11.84 TSAAGPGDD844 pKa = 3.63 VSLSSTLNNPDD855 pKa = 3.26 QTSVSAVNSEE865 pKa = 4.59 DD866 pKa = 3.26 ISLEE870 pKa = 3.88 PTLIGPDD877 pKa = 3.34 QTSASIGDD885 pKa = 3.74 ASLTDD890 pKa = 4.09 SPPSVPTTFVPGVNPPPLPSSPFNPGNLSRR920 pKa = 11.84 SATFEE925 pKa = 4.67 AIVTTVVYY933 pKa = 9.26 TVLDD937 pKa = 3.83 PHH939 pKa = 7.16 DD940 pKa = 4.12 PAYY943 pKa = 10.01 LTVAEE948 pKa = 4.79 FCTTLKK954 pKa = 10.82 YY955 pKa = 10.16 PPCRR959 pKa = 11.84 NCQFQRR965 pKa = 11.84 LPTVEE970 pKa = 3.95 MTTILTACDD979 pKa = 2.88 ACGYY983 pKa = 9.94 HH984 pKa = 6.44 GEE986 pKa = 4.13 NSIEE990 pKa = 3.86 LTIPLGANPALMTSNPYY1007 pKa = 9.64 TPDD1010 pKa = 3.74 DD1011 pKa = 3.97 VPGGSPAAAHH1021 pKa = 6.98 DD1022 pKa = 4.35 EE1023 pKa = 4.31 NSGPEE1028 pKa = 3.97 YY1029 pKa = 10.39 RR1030 pKa = 11.84 PRR1032 pKa = 11.84 PGGAEE1037 pKa = 3.68 ARR1039 pKa = 11.84 PTVVPVGHH1047 pKa = 7.09 PPYY1050 pKa = 9.94 GGHH1053 pKa = 6.84 DD1054 pKa = 3.3 RR1055 pKa = 11.84 PVIHH1059 pKa = 7.2 HH1060 pKa = 6.86 EE1061 pKa = 4.27 GTTGEE1066 pKa = 4.24 HH1067 pKa = 5.75 NKK1069 pKa = 9.8 EE1070 pKa = 4.01 NKK1072 pKa = 9.84 PPGDD1076 pKa = 3.1 NHH1078 pKa = 7.69 APIGEE1083 pKa = 4.52 PGPSQHH1089 pKa = 5.95 EE1090 pKa = 4.09 QVPSNHH1096 pKa = 6.6 HH1097 pKa = 6.49 GEE1099 pKa = 4.28 PAGPEE1104 pKa = 3.99 YY1105 pKa = 10.44 EE1106 pKa = 4.14 PQIGPGKK1113 pKa = 10.37 DD1114 pKa = 3.1 GATVKK1119 pKa = 9.09 PTLAPSVEE1127 pKa = 4.22 AVPKK1131 pKa = 9.94 PEE1133 pKa = 4.11 PAGASADD1140 pKa = 3.48 LKK1142 pKa = 11.21 LFTVPMANIPTTPPDD1157 pKa = 3.4 SPVIVAAGHH1166 pKa = 4.75 VARR1169 pKa = 11.84 GIDD1172 pKa = 3.64 GVLTLFALVAGFFLMLL1188 pKa = 4.56
Molecular weight: 122.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.961
Patrickios 1.583
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|C7YRP9|C7YRP9_FUSV7 Uncharacterized protein CHR2115 OS=Fusarium vanettenii (strain ATCC MYA-4622 / CBS 123669 / FGSC 9596 / NRRL 45880 / 77-13-4) OX=660122 GN=CHR2115 PE=3 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.65 RR8 pKa = 11.84 QTTPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APARR26 pKa = 11.84 SHH28 pKa = 5.86 HH29 pKa = 6.11 TVTTTTTTTRR39 pKa = 11.84 KK40 pKa = 8.7 PRR42 pKa = 11.84 RR43 pKa = 11.84 GFLGGGRR50 pKa = 11.84 RR51 pKa = 11.84 THH53 pKa = 7.04 AAPVHH58 pKa = 5.1 HH59 pKa = 5.69 QQRR62 pKa = 11.84 RR63 pKa = 11.84 PSMKK67 pKa = 10.24 DD68 pKa = 2.95 KK69 pKa = 11.37 VSGALLRR76 pKa = 11.84 LKK78 pKa = 10.81 GSLTRR83 pKa = 11.84 RR84 pKa = 11.84 PGVKK88 pKa = 9.89 AAGTRR93 pKa = 11.84 RR94 pKa = 11.84 MHH96 pKa = 5.68 GTDD99 pKa = 2.6 GRR101 pKa = 11.84 GSHH104 pKa = 5.81 RR105 pKa = 11.84 RR106 pKa = 11.84 ARR108 pKa = 11.84 HH109 pKa = 4.06 FF110 pKa = 3.77
Molecular weight: 12.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.49
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.442
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.149
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15709
0
15709
7536017
48
7649
479.7
53.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.48 ± 0.02
1.354 ± 0.008
5.815 ± 0.015
6.178 ± 0.019
3.891 ± 0.012
6.942 ± 0.017
2.392 ± 0.01
5.029 ± 0.011
4.833 ± 0.016
9.045 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.007
3.589 ± 0.01
5.943 ± 0.018
3.894 ± 0.013
5.958 ± 0.017
7.865 ± 0.019
5.882 ± 0.016
6.235 ± 0.014
1.647 ± 0.007
2.803 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here