Streptomyces phage phiCAM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Camvirus; Streptomyces virus phiCAM

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4PAV4|K4PAV4_9CAUD Uncharacterized protein OS=Streptomyces phage phiCAM OX=1239386 PE=4 SV=1
MM1 pKa = 7.81SIYY4 pKa = 8.27TTEE7 pKa = 3.89ALADD11 pKa = 3.37IARR14 pKa = 11.84VLKK17 pKa = 9.96TDD19 pKa = 3.76EE20 pKa = 4.35LPEE23 pKa = 4.42EE24 pKa = 3.95IVAIVNSLVEE34 pKa = 4.16NEE36 pKa = 4.39RR37 pKa = 11.84QDD39 pKa = 4.71AYY41 pKa = 11.2DD42 pKa = 4.65DD43 pKa = 3.94GHH45 pKa = 6.39TDD47 pKa = 3.8GYY49 pKa = 11.39SEE51 pKa = 5.41GEE53 pKa = 3.76DD54 pKa = 3.27FAYY57 pKa = 11.02NEE59 pKa = 5.01GYY61 pKa = 10.38DD62 pKa = 3.28AGRR65 pKa = 11.84SEE67 pKa = 5.75GYY69 pKa = 10.48DD70 pKa = 3.14EE71 pKa = 4.51GHH73 pKa = 7.06ADD75 pKa = 4.38GYY77 pKa = 11.48DD78 pKa = 3.13EE79 pKa = 4.52GVNDD83 pKa = 3.63GRR85 pKa = 11.84EE86 pKa = 3.94EE87 pKa = 4.24AEE89 pKa = 3.69QDD91 pKa = 3.28AAA93 pKa = 4.73

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4NZT9|K4NZT9_9CAUD HNHc domain-containing protein OS=Streptomyces phage phiCAM OX=1239386 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 9.32TKK5 pKa = 10.06TVDD8 pKa = 4.07FRR10 pKa = 11.84YY11 pKa = 10.16CEE13 pKa = 4.39CGLKK17 pKa = 10.34RR18 pKa = 11.84GFRR21 pKa = 11.84TEE23 pKa = 3.72RR24 pKa = 11.84DD25 pKa = 2.93ADD27 pKa = 3.74KK28 pKa = 11.41ALGRR32 pKa = 11.84AQAKK36 pKa = 9.35RR37 pKa = 11.84DD38 pKa = 3.43RR39 pKa = 11.84AAEE42 pKa = 3.76ARR44 pKa = 11.84GTRR47 pKa = 11.84RR48 pKa = 11.84GSHH51 pKa = 5.7RR52 pKa = 11.84EE53 pKa = 3.71SRR55 pKa = 11.84FYY57 pKa = 11.38ACDD60 pKa = 3.46FGLFHH65 pKa = 6.9LTSEE69 pKa = 4.49SRR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 5.18FNDD76 pKa = 3.04RR77 pKa = 11.84LVAAA81 pKa = 5.45

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13684

39

1323

190.1

20.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.356 ± 0.422

0.702 ± 0.118

6.255 ± 0.276

6.65 ± 0.454

3.062 ± 0.238

8.726 ± 0.386

1.98 ± 0.197

4.312 ± 0.337

4.567 ± 0.323

8.272 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.174

3.12 ± 0.153

4.86 ± 0.239

3.128 ± 0.167

6.226 ± 0.48

5.978 ± 0.294

6.738 ± 0.393

7.001 ± 0.232

2.061 ± 0.152

2.777 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski