Amino acid dipepetide frequency for Streptomyces phage phiCAM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.886AlaAla: 13.886 ± 1.321
0.731AlaCys: 0.731 ± 0.213
7.162AlaAsp: 7.162 ± 0.693
7.601AlaGlu: 7.601 ± 0.84
3.143AlaPhe: 3.143 ± 0.465
8.916AlaGly: 8.916 ± 0.899
1.316AlaHis: 1.316 ± 0.321
4.677AlaIle: 4.677 ± 0.789
5.043AlaLys: 5.043 ± 0.65
10.963AlaLeu: 10.963 ± 1.439
2.923AlaMet: 2.923 ± 0.482
3.508AlaAsn: 3.508 ± 0.513
4.897AlaPro: 4.897 ± 0.697
4.458AlaGln: 4.458 ± 0.574
6.578AlaArg: 6.578 ± 0.687
5.847AlaSer: 5.847 ± 0.683
6.724AlaThr: 6.724 ± 0.837
8.916AlaVal: 8.916 ± 0.921
2.704AlaTrp: 2.704 ± 0.411
3.581AlaTyr: 3.581 ± 0.628
0.0AlaXaa: 0.0 ± 0.0
Cys
0.219CysAla: 0.219 ± 0.131
0.073CysCys: 0.073 ± 0.079
0.585CysAsp: 0.585 ± 0.199
0.365CysGlu: 0.365 ± 0.177
0.219CysPhe: 0.219 ± 0.133
1.023CysGly: 1.023 ± 0.236
0.439CysHis: 0.439 ± 0.215
0.146CysIle: 0.146 ± 0.095
0.219CysLys: 0.219 ± 0.116
0.585CysLeu: 0.585 ± 0.204
0.073CysMet: 0.073 ± 0.062
0.219CysAsn: 0.219 ± 0.109
0.658CysPro: 0.658 ± 0.268
0.146CysGln: 0.146 ± 0.11
0.365CysArg: 0.365 ± 0.191
0.439CysSer: 0.439 ± 0.16
0.365CysThr: 0.365 ± 0.168
0.439CysVal: 0.439 ± 0.202
0.146CysTrp: 0.146 ± 0.098
0.292CysTyr: 0.292 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
6.212AspAla: 6.212 ± 0.732
0.658AspCys: 0.658 ± 0.254
3.508AspAsp: 3.508 ± 0.628
5.262AspGlu: 5.262 ± 0.853
1.681AspPhe: 1.681 ± 0.347
6.943AspGly: 6.943 ± 0.734
1.169AspHis: 1.169 ± 0.32
3.727AspIle: 3.727 ± 0.59
2.119AspLys: 2.119 ± 0.514
5.408AspLeu: 5.408 ± 0.804
1.389AspMet: 1.389 ± 0.353
1.608AspAsn: 1.608 ± 0.38
3.07AspPro: 3.07 ± 0.553
2.558AspGln: 2.558 ± 0.537
3.143AspArg: 3.143 ± 0.52
2.704AspSer: 2.704 ± 0.439
3.947AspThr: 3.947 ± 0.484
4.75AspVal: 4.75 ± 0.555
1.535AspTrp: 1.535 ± 0.313
1.169AspTyr: 1.169 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
7.82GluAla: 7.82 ± 0.996
0.804GluCys: 0.804 ± 0.291
4.897GluAsp: 4.897 ± 0.722
3.435GluGlu: 3.435 ± 0.669
2.266GluPhe: 2.266 ± 0.408
6.431GluGly: 6.431 ± 0.775
1.462GluHis: 1.462 ± 0.372
2.777GluIle: 2.777 ± 0.553
2.631GluLys: 2.631 ± 0.397
6.724GluLeu: 6.724 ± 0.694
1.023GluMet: 1.023 ± 0.288
1.9GluAsn: 1.9 ± 0.35
2.923GluPro: 2.923 ± 0.517
2.558GluGln: 2.558 ± 0.433
4.677GluArg: 4.677 ± 1.036
3.508GluSer: 3.508 ± 0.557
3.289GluThr: 3.289 ± 0.461
4.093GluVal: 4.093 ± 0.573
1.462GluTrp: 1.462 ± 0.344
1.827GluTyr: 1.827 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
3.362PheAla: 3.362 ± 0.456
0.219PheCys: 0.219 ± 0.111
1.973PheAsp: 1.973 ± 0.361
1.973PheGlu: 1.973 ± 0.358
1.023PhePhe: 1.023 ± 0.236
3.07PheGly: 3.07 ± 0.414
0.512PheHis: 0.512 ± 0.172
1.023PheIle: 1.023 ± 0.237
1.462PheLys: 1.462 ± 0.37
2.119PheLeu: 2.119 ± 0.55
0.804PheMet: 0.804 ± 0.258
1.242PheAsn: 1.242 ± 0.296
1.462PhePro: 1.462 ± 0.289
0.585PheGln: 0.585 ± 0.208
2.339PheArg: 2.339 ± 0.412
1.608PheSer: 1.608 ± 0.411
2.046PheThr: 2.046 ± 0.37
1.973PheVal: 1.973 ± 0.44
0.731PheTrp: 0.731 ± 0.192
1.096PheTyr: 1.096 ± 0.304
0.0PheXaa: 0.0 ± 0.0
Gly
8.478GlyAla: 8.478 ± 1.075
0.073GlyCys: 0.073 ± 0.077
5.92GlyAsp: 5.92 ± 0.841
5.262GlyGlu: 5.262 ± 0.891
2.777GlyPhe: 2.777 ± 0.479
7.235GlyGly: 7.235 ± 0.834
2.119GlyHis: 2.119 ± 0.502
4.385GlyIle: 4.385 ± 0.991
5.189GlyLys: 5.189 ± 0.68
6.724GlyLeu: 6.724 ± 0.7
2.119GlyMet: 2.119 ± 0.375
2.266GlyAsn: 2.266 ± 0.399
3.727GlyPro: 3.727 ± 0.678
2.923GlyGln: 2.923 ± 0.54
4.312GlyArg: 4.312 ± 0.535
5.408GlySer: 5.408 ± 0.883
6.212GlyThr: 6.212 ± 0.805
7.162GlyVal: 7.162 ± 0.626
2.339GlyTrp: 2.339 ± 0.438
2.631GlyTyr: 2.631 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.754HisAla: 1.754 ± 0.332
0.365HisCys: 0.365 ± 0.166
0.95HisAsp: 0.95 ± 0.312
1.023HisGlu: 1.023 ± 0.279
1.389HisPhe: 1.389 ± 0.277
1.169HisGly: 1.169 ± 0.317
0.731HisHis: 0.731 ± 0.245
0.658HisIle: 0.658 ± 0.214
0.292HisLys: 0.292 ± 0.166
2.119HisLeu: 2.119 ± 0.559
0.219HisMet: 0.219 ± 0.116
0.585HisAsn: 0.585 ± 0.225
1.316HisPro: 1.316 ± 0.366
0.512HisGln: 0.512 ± 0.209
1.608HisArg: 1.608 ± 0.416
1.169HisSer: 1.169 ± 0.39
1.316HisThr: 1.316 ± 0.348
0.95HisVal: 0.95 ± 0.348
0.731HisTrp: 0.731 ± 0.285
0.95HisTyr: 0.95 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
5.701IleAla: 5.701 ± 0.663
0.292IleCys: 0.292 ± 0.129
2.631IleAsp: 2.631 ± 0.462
3.654IleGlu: 3.654 ± 0.493
0.877IlePhe: 0.877 ± 0.249
3.947IleGly: 3.947 ± 0.516
0.512IleHis: 0.512 ± 0.21
1.9IleIle: 1.9 ± 0.865
1.608IleLys: 1.608 ± 0.409
2.923IleLeu: 2.923 ± 0.632
0.585IleMet: 0.585 ± 0.211
1.242IleAsn: 1.242 ± 0.32
2.266IlePro: 2.266 ± 0.391
1.681IleGln: 1.681 ± 0.38
2.558IleArg: 2.558 ± 0.386
2.485IleSer: 2.485 ± 0.493
2.777IleThr: 2.777 ± 0.553
3.362IleVal: 3.362 ± 0.58
0.585IleTrp: 0.585 ± 0.211
1.535IleTyr: 1.535 ± 0.325
0.0IleXaa: 0.0 ± 0.0
Lys
6.724LysAla: 6.724 ± 0.909
0.073LysCys: 0.073 ± 0.077
2.631LysAsp: 2.631 ± 0.418
1.9LysGlu: 1.9 ± 0.318
1.389LysPhe: 1.389 ± 0.325
4.166LysGly: 4.166 ± 0.472
1.169LysHis: 1.169 ± 0.28
2.193LysIle: 2.193 ± 0.403
2.485LysLys: 2.485 ± 0.421
3.435LysLeu: 3.435 ± 0.577
1.316LysMet: 1.316 ± 0.319
1.096LysAsn: 1.096 ± 0.31
3.435LysPro: 3.435 ± 0.752
0.95LysGln: 0.95 ± 0.28
2.631LysArg: 2.631 ± 0.469
2.339LysSer: 2.339 ± 0.474
3.362LysThr: 3.362 ± 0.496
3.07LysVal: 3.07 ± 0.577
0.365LysTrp: 0.365 ± 0.132
0.95LysTyr: 0.95 ± 0.314
0.0LysXaa: 0.0 ± 0.0
Leu
11.036LeuAla: 11.036 ± 1.128
0.585LeuCys: 0.585 ± 0.222
5.481LeuAsp: 5.481 ± 0.598
4.604LeuGlu: 4.604 ± 0.661
1.462LeuPhe: 1.462 ± 0.335
7.674LeuGly: 7.674 ± 0.94
1.023LeuHis: 1.023 ± 0.318
3.508LeuIle: 3.508 ± 0.556
4.02LeuLys: 4.02 ± 0.771
6.066LeuLeu: 6.066 ± 0.803
1.827LeuMet: 1.827 ± 0.404
3.727LeuAsn: 3.727 ± 0.633
3.947LeuPro: 3.947 ± 0.729
1.827LeuGln: 1.827 ± 0.296
5.774LeuArg: 5.774 ± 0.874
6.578LeuSer: 6.578 ± 0.639
4.677LeuThr: 4.677 ± 0.445
5.043LeuVal: 5.043 ± 0.607
1.242LeuTrp: 1.242 ± 0.3
2.631LeuTyr: 2.631 ± 0.401
0.0LeuXaa: 0.0 ± 0.0
Met
2.777MetAla: 2.777 ± 0.442
0.073MetCys: 0.073 ± 0.062
0.877MetAsp: 0.877 ± 0.24
1.389MetGlu: 1.389 ± 0.381
0.877MetPhe: 0.877 ± 0.368
1.535MetGly: 1.535 ± 0.332
0.292MetHis: 0.292 ± 0.146
1.316MetIle: 1.316 ± 0.349
1.242MetLys: 1.242 ± 0.285
2.046MetLeu: 2.046 ± 0.405
0.292MetMet: 0.292 ± 0.143
0.731MetAsn: 0.731 ± 0.208
1.242MetPro: 1.242 ± 0.335
0.731MetGln: 0.731 ± 0.261
1.316MetArg: 1.316 ± 0.395
2.193MetSer: 2.193 ± 0.385
1.9MetThr: 1.9 ± 0.387
1.096MetVal: 1.096 ± 0.213
0.146MetTrp: 0.146 ± 0.096
0.219MetTyr: 0.219 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
3.289AsnAla: 3.289 ± 0.489
0.439AsnCys: 0.439 ± 0.222
2.193AsnAsp: 2.193 ± 0.341
2.119AsnGlu: 2.119 ± 0.396
1.023AsnPhe: 1.023 ± 0.295
2.923AsnGly: 2.923 ± 0.501
0.658AsnHis: 0.658 ± 0.26
0.804AsnIle: 0.804 ± 0.22
1.316AsnLys: 1.316 ± 0.348
3.07AsnLeu: 3.07 ± 0.477
0.512AsnMet: 0.512 ± 0.256
1.023AsnAsn: 1.023 ± 0.289
1.608AsnPro: 1.608 ± 0.347
0.95AsnGln: 0.95 ± 0.3
1.827AsnArg: 1.827 ± 0.324
1.389AsnSer: 1.389 ± 0.365
2.996AsnThr: 2.996 ± 0.556
1.827AsnVal: 1.827 ± 0.362
0.365AsnTrp: 0.365 ± 0.18
0.877AsnTyr: 0.877 ± 0.196
0.0AsnXaa: 0.0 ± 0.0
Pro
4.97ProAla: 4.97 ± 0.587
0.585ProCys: 0.585 ± 0.186
2.85ProAsp: 2.85 ± 0.409
4.458ProGlu: 4.458 ± 0.621
1.169ProPhe: 1.169 ± 0.321
4.093ProGly: 4.093 ± 0.531
0.731ProHis: 0.731 ± 0.235
2.266ProIle: 2.266 ± 0.57
2.631ProLys: 2.631 ± 0.538
2.85ProLeu: 2.85 ± 0.638
1.169ProMet: 1.169 ± 0.269
1.535ProAsn: 1.535 ± 0.354
2.119ProPro: 2.119 ± 0.542
1.096ProGln: 1.096 ± 0.389
2.631ProArg: 2.631 ± 0.455
3.581ProSer: 3.581 ± 0.552
4.02ProThr: 4.02 ± 0.784
3.727ProVal: 3.727 ± 0.464
1.023ProTrp: 1.023 ± 0.243
1.096ProTyr: 1.096 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
4.239GlnAla: 4.239 ± 0.668
0.219GlnCys: 0.219 ± 0.124
1.316GlnAsp: 1.316 ± 0.385
1.973GlnGlu: 1.973 ± 0.48
1.023GlnPhe: 1.023 ± 0.312
2.193GlnGly: 2.193 ± 0.525
0.585GlnHis: 0.585 ± 0.235
1.535GlnIle: 1.535 ± 0.302
1.608GlnLys: 1.608 ± 0.38
2.266GlnLeu: 2.266 ± 0.502
1.023GlnMet: 1.023 ± 0.337
1.169GlnAsn: 1.169 ± 0.293
1.316GlnPro: 1.316 ± 0.334
0.585GlnGln: 0.585 ± 0.199
2.485GlnArg: 2.485 ± 0.504
1.681GlnSer: 1.681 ± 0.338
2.046GlnThr: 2.046 ± 0.548
2.339GlnVal: 2.339 ± 0.435
0.731GlnTrp: 0.731 ± 0.22
0.95GlnTyr: 0.95 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
5.481ArgAla: 5.481 ± 0.632
0.439ArgCys: 0.439 ± 0.169
3.654ArgAsp: 3.654 ± 0.729
4.824ArgGlu: 4.824 ± 0.838
2.412ArgPhe: 2.412 ± 0.498
4.239ArgGly: 4.239 ± 0.511
1.096ArgHis: 1.096 ± 0.288
2.339ArgIle: 2.339 ± 0.437
3.727ArgLys: 3.727 ± 0.627
6.651ArgLeu: 6.651 ± 0.868
1.608ArgMet: 1.608 ± 0.37
1.9ArgAsn: 1.9 ± 0.338
2.266ArgPro: 2.266 ± 0.637
2.119ArgGln: 2.119 ± 0.502
5.554ArgArg: 5.554 ± 1.013
3.435ArgSer: 3.435 ± 0.532
4.02ArgThr: 4.02 ± 0.537
3.435ArgVal: 3.435 ± 0.573
1.242ArgTrp: 1.242 ± 0.359
1.827ArgTyr: 1.827 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
7.016SerAla: 7.016 ± 0.772
0.146SerCys: 0.146 ± 0.115
3.654SerAsp: 3.654 ± 0.499
4.166SerGlu: 4.166 ± 0.543
1.754SerPhe: 1.754 ± 0.315
5.189SerGly: 5.189 ± 0.953
1.242SerHis: 1.242 ± 0.262
2.558SerIle: 2.558 ± 0.575
1.973SerLys: 1.973 ± 0.381
4.97SerLeu: 4.97 ± 0.587
1.023SerMet: 1.023 ± 0.294
1.9SerAsn: 1.9 ± 0.31
2.996SerPro: 2.996 ± 0.546
1.754SerGln: 1.754 ± 0.344
3.508SerArg: 3.508 ± 0.496
3.362SerSer: 3.362 ± 0.498
4.897SerThr: 4.897 ± 0.684
4.531SerVal: 4.531 ± 0.572
1.681SerTrp: 1.681 ± 0.422
1.462SerTyr: 1.462 ± 0.3
0.0SerXaa: 0.0 ± 0.0
Thr
6.724ThrAla: 6.724 ± 0.908
0.585ThrCys: 0.585 ± 0.179
4.02ThrAsp: 4.02 ± 0.681
3.947ThrGlu: 3.947 ± 0.684
3.362ThrPhe: 3.362 ± 0.587
6.066ThrGly: 6.066 ± 0.86
1.827ThrHis: 1.827 ± 0.542
2.631ThrIle: 2.631 ± 0.414
2.704ThrLys: 2.704 ± 0.42
5.92ThrLeu: 5.92 ± 0.74
1.242ThrMet: 1.242 ± 0.238
1.462ThrAsn: 1.462 ± 0.321
3.947ThrPro: 3.947 ± 0.785
2.266ThrGln: 2.266 ± 0.514
3.508ThrArg: 3.508 ± 0.54
4.531ThrSer: 4.531 ± 0.731
4.458ThrThr: 4.458 ± 0.685
4.824ThrVal: 4.824 ± 0.559
1.316ThrTrp: 1.316 ± 0.323
2.046ThrTyr: 2.046 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
7.747ValAla: 7.747 ± 0.644
0.219ValCys: 0.219 ± 0.118
4.239ValAsp: 4.239 ± 0.503
4.897ValGlu: 4.897 ± 0.749
1.462ValPhe: 1.462 ± 0.317
5.701ValGly: 5.701 ± 0.746
1.827ValHis: 1.827 ± 0.383
3.216ValIle: 3.216 ± 0.484
3.508ValLys: 3.508 ± 0.502
4.97ValLeu: 4.97 ± 0.838
1.973ValMet: 1.973 ± 0.376
2.266ValAsn: 2.266 ± 0.474
3.727ValPro: 3.727 ± 0.499
2.412ValGln: 2.412 ± 0.497
3.581ValArg: 3.581 ± 0.683
4.458ValSer: 4.458 ± 0.588
5.408ValThr: 5.408 ± 0.703
5.481ValVal: 5.481 ± 0.653
1.169ValTrp: 1.169 ± 0.316
1.681ValTyr: 1.681 ± 0.341
0.0ValXaa: 0.0 ± 0.0
Trp
2.412TrpAla: 2.412 ± 0.399
0.219TrpCys: 0.219 ± 0.13
1.827TrpAsp: 1.827 ± 0.404
1.096TrpGlu: 1.096 ± 0.283
0.512TrpPhe: 0.512 ± 0.201
1.535TrpGly: 1.535 ± 0.284
0.585TrpHis: 0.585 ± 0.212
0.365TrpIle: 0.365 ± 0.164
1.169TrpLys: 1.169 ± 0.282
1.242TrpLeu: 1.242 ± 0.28
0.658TrpMet: 0.658 ± 0.189
0.877TrpAsn: 0.877 ± 0.254
0.585TrpPro: 0.585 ± 0.213
0.512TrpGln: 0.512 ± 0.153
1.827TrpArg: 1.827 ± 0.417
1.169TrpSer: 1.169 ± 0.35
1.608TrpThr: 1.608 ± 0.386
1.535TrpVal: 1.535 ± 0.263
0.439TrpTrp: 0.439 ± 0.191
0.439TrpTyr: 0.439 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.727TyrAla: 3.727 ± 0.434
0.219TyrCys: 0.219 ± 0.127
2.193TyrAsp: 2.193 ± 0.46
2.558TyrGlu: 2.558 ± 0.459
0.804TyrPhe: 0.804 ± 0.257
3.216TyrGly: 3.216 ± 0.625
0.512TyrHis: 0.512 ± 0.23
1.096TyrIle: 1.096 ± 0.28
0.731TyrLys: 0.731 ± 0.258
1.681TyrLeu: 1.681 ± 0.353
0.439TyrMet: 0.439 ± 0.165
0.95TyrAsn: 0.95 ± 0.243
1.096TyrPro: 1.096 ± 0.283
0.658TyrGln: 0.658 ± 0.224
2.046TyrArg: 2.046 ± 0.44
1.9TyrSer: 1.9 ± 0.394
1.316TyrThr: 1.316 ± 0.326
1.462TyrVal: 1.462 ± 0.367
0.658TyrTrp: 0.658 ± 0.213
0.512TyrTyr: 0.512 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13684 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski