Bacillus phage Mater

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Matervirus; Bacillus virus Mater

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RMU8|A0A0A0RMU8_9CAUD Baseplate protein OS=Bacillus phage Mater OX=1540090 GN=CPT_Mater155 PE=4 SV=1
MM1 pKa = 7.28TNVNVTEE8 pKa = 4.07MEE10 pKa = 4.28KK11 pKa = 10.91AVMDD15 pKa = 4.5VIKK18 pKa = 10.17STMDD22 pKa = 3.62DD23 pKa = 3.77YY24 pKa = 11.96CDD26 pKa = 3.74GFSDD30 pKa = 5.03VMTEE34 pKa = 4.24DD35 pKa = 3.94LVSATGYY42 pKa = 11.14KK43 pKa = 8.82MNQVKK48 pKa = 10.64GILSSLDD55 pKa = 3.13KK56 pKa = 11.12KK57 pKa = 10.93GFVYY61 pKa = 10.68FMDD64 pKa = 3.6VNGEE68 pKa = 4.27YY69 pKa = 10.75NVFALTQTGADD80 pKa = 3.14ALGYY84 pKa = 8.91EE85 pKa = 4.18LEE87 pKa = 4.4YY88 pKa = 11.3YY89 pKa = 10.08EE90 pKa = 5.09EE91 pKa = 4.61LMGG94 pKa = 5.5

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RUT1|A0A0A0RUT1_9CAUD Uncharacterized protein OS=Bacillus phage Mater OX=1540090 GN=CPT_Mater181 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84KK4 pKa = 9.26KK5 pKa = 10.52ARR7 pKa = 11.84RR8 pKa = 11.84PKK10 pKa = 10.47LFQNAQAPKK19 pKa = 9.86QAMKK23 pKa = 10.81NVGNALVQKK32 pKa = 9.5TLDD35 pKa = 3.56AGMKK39 pKa = 9.46AAQAEE44 pKa = 4.43KK45 pKa = 10.41PKK47 pKa = 10.26DD48 pKa = 3.46AKK50 pKa = 10.47VVRR53 pKa = 11.84KK54 pKa = 9.03PKK56 pKa = 9.65YY57 pKa = 10.39LEE59 pKa = 3.93VTEE62 pKa = 4.1TRR64 pKa = 11.84MNKK67 pKa = 10.23LGVIDD72 pKa = 4.52LKK74 pKa = 11.08PYY76 pKa = 10.44FSRR79 pKa = 11.84SSHH82 pKa = 5.78KK83 pKa = 10.69VMKK86 pKa = 10.58KK87 pKa = 9.78GGGWYY92 pKa = 10.2LRR94 pKa = 11.84VPIKK98 pKa = 10.43RR99 pKa = 11.84KK100 pKa = 10.01KK101 pKa = 10.02KK102 pKa = 10.01DD103 pKa = 3.15MSKK106 pKa = 10.69RR107 pKa = 11.84MYY109 pKa = 8.7TQLRR113 pKa = 11.84TTSLGEE119 pKa = 3.89SDD121 pKa = 3.69SRR123 pKa = 11.84TIISDD128 pKa = 3.27YY129 pKa = 10.88LYY131 pKa = 10.8DD132 pKa = 3.57RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84ASGSNMLDD143 pKa = 3.55YY144 pKa = 11.02SPKK147 pKa = 9.98SYY149 pKa = 10.98NITRR153 pKa = 11.84QKK155 pKa = 8.89TGSRR159 pKa = 11.84KK160 pKa = 9.35SVYY163 pKa = 8.78IAYY166 pKa = 7.91RR167 pKa = 11.84TVSNNSPASSWIINRR182 pKa = 11.84DD183 pKa = 3.27RR184 pKa = 11.84VNSDD188 pKa = 3.18DD189 pKa = 3.57TSKK192 pKa = 9.93TFVRR196 pKa = 11.84NMNRR200 pKa = 11.84LMKK203 pKa = 9.94WKK205 pKa = 9.3MKK207 pKa = 10.65NGWEE211 pKa = 3.98

Molecular weight: 24.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

222

0

222

46840

27

1319

211.0

23.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.005 ± 0.176

0.666 ± 0.069

6.27 ± 0.143

7.605 ± 0.278

3.992 ± 0.099

6.674 ± 0.245

1.83 ± 0.1

6.452 ± 0.141

7.408 ± 0.185

7.963 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.09

5.506 ± 0.154

3.266 ± 0.119

3.813 ± 0.143

4.281 ± 0.121

5.822 ± 0.174

6.52 ± 0.218

6.768 ± 0.163

0.997 ± 0.055

4.46 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski