Amino acid dipepetide frequency for Bacillus phage Mater

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.893AlaAla: 5.893 ± 0.401
0.406AlaCys: 0.406 ± 0.086
4.676AlaAsp: 4.676 ± 0.369
5.124AlaGlu: 5.124 ± 0.376
2.989AlaPhe: 2.989 ± 0.248
5.017AlaGly: 5.017 ± 0.369
1.067AlaHis: 1.067 ± 0.165
4.547AlaIle: 4.547 ± 0.327
5.167AlaLys: 5.167 ± 0.335
6.085AlaLeu: 6.085 ± 0.419
1.879AlaMet: 1.879 ± 0.228
3.053AlaAsn: 3.053 ± 0.271
2.583AlaPro: 2.583 ± 0.349
2.818AlaGln: 2.818 ± 0.291
3.053AlaArg: 3.053 ± 0.243
3.288AlaSer: 3.288 ± 0.281
4.483AlaThr: 4.483 ± 0.319
4.355AlaVal: 4.355 ± 0.303
0.79AlaTrp: 0.79 ± 0.146
2.775AlaTyr: 2.775 ± 0.31
0.0AlaXaa: 0.0 ± 0.0
Cys
0.256CysAla: 0.256 ± 0.069
0.064CysCys: 0.064 ± 0.038
0.512CysAsp: 0.512 ± 0.116
0.448CysGlu: 0.448 ± 0.095
0.278CysPhe: 0.278 ± 0.08
0.555CysGly: 0.555 ± 0.126
0.149CysHis: 0.149 ± 0.06
0.576CysIle: 0.576 ± 0.109
0.448CysLys: 0.448 ± 0.109
0.448CysLeu: 0.448 ± 0.089
0.128CysMet: 0.128 ± 0.05
0.342CysAsn: 0.342 ± 0.086
0.299CysPro: 0.299 ± 0.1
0.149CysGln: 0.149 ± 0.06
0.256CysArg: 0.256 ± 0.08
0.448CysSer: 0.448 ± 0.103
0.406CysThr: 0.406 ± 0.091
0.32CysVal: 0.32 ± 0.073
0.128CysTrp: 0.128 ± 0.06
0.448CysTyr: 0.448 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
4.27AspAla: 4.27 ± 0.355
0.448AspCys: 0.448 ± 0.094
3.501AspAsp: 3.501 ± 0.351
4.142AspGlu: 4.142 ± 0.361
2.669AspPhe: 2.669 ± 0.257
4.313AspGly: 4.313 ± 0.356
1.003AspHis: 1.003 ± 0.145
4.697AspIle: 4.697 ± 0.345
5.38AspLys: 5.38 ± 0.347
4.74AspLeu: 4.74 ± 0.305
2.199AspMet: 2.199 ± 0.239
3.608AspAsn: 3.608 ± 0.27
1.986AspPro: 1.986 ± 0.219
1.964AspGln: 1.964 ± 0.199
2.519AspArg: 2.519 ± 0.262
3.181AspSer: 3.181 ± 0.292
4.099AspThr: 4.099 ± 0.327
4.291AspVal: 4.291 ± 0.295
0.79AspTrp: 0.79 ± 0.131
2.904AspTyr: 2.904 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
5.209GluAla: 5.209 ± 0.367
0.427GluCys: 0.427 ± 0.103
4.697GluAsp: 4.697 ± 0.374
8.092GluGlu: 8.092 ± 0.764
2.775GluPhe: 2.775 ± 0.267
5.359GluGly: 5.359 ± 0.364
1.281GluHis: 1.281 ± 0.174
5.124GluIle: 5.124 ± 0.417
5.423GluLys: 5.423 ± 0.512
7.28GluLeu: 7.28 ± 0.454
2.69GluMet: 2.69 ± 0.245
3.565GluAsn: 3.565 ± 0.277
1.559GluPro: 1.559 ± 0.209
3.309GluGln: 3.309 ± 0.299
3.202GluArg: 3.202 ± 0.333
2.968GluSer: 2.968 ± 0.271
3.416GluThr: 3.416 ± 0.256
5.273GluVal: 5.273 ± 0.346
0.939GluTrp: 0.939 ± 0.134
3.437GluTyr: 3.437 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.242PheAla: 2.242 ± 0.217
0.427PheCys: 0.427 ± 0.115
2.754PheAsp: 2.754 ± 0.245
2.562PheGlu: 2.562 ± 0.21
1.473PhePhe: 1.473 ± 0.187
2.391PheGly: 2.391 ± 0.267
0.875PheHis: 0.875 ± 0.147
3.395PheIle: 3.395 ± 0.278
2.818PheLys: 2.818 ± 0.261
2.69PheLeu: 2.69 ± 0.24
0.982PheMet: 0.982 ± 0.157
2.477PheAsn: 2.477 ± 0.284
1.196PhePro: 1.196 ± 0.146
1.345PheGln: 1.345 ± 0.179
1.452PheArg: 1.452 ± 0.199
3.096PheSer: 3.096 ± 0.306
3.01PheThr: 3.01 ± 0.294
2.583PheVal: 2.583 ± 0.209
0.32PheTrp: 0.32 ± 0.073
1.836PheTyr: 1.836 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
4.419GlyAla: 4.419 ± 0.381
0.555GlyCys: 0.555 ± 0.12
3.95GlyAsp: 3.95 ± 0.299
3.864GlyGlu: 3.864 ± 0.261
2.647GlyPhe: 2.647 ± 0.278
5.401GlyGly: 5.401 ± 0.747
1.324GlyHis: 1.324 ± 0.185
3.779GlyIle: 3.779 ± 0.287
4.889GlyLys: 4.889 ± 0.344
5.252GlyLeu: 5.252 ± 0.345
1.751GlyMet: 1.751 ± 0.228
3.971GlyAsn: 3.971 ± 0.336
1.132GlyPro: 1.132 ± 0.213
2.22GlyGln: 2.22 ± 0.177
2.69GlyArg: 2.69 ± 0.205
4.526GlySer: 4.526 ± 0.352
5.188GlyThr: 5.188 ± 0.41
4.547GlyVal: 4.547 ± 0.286
0.897GlyTrp: 0.897 ± 0.157
3.736GlyTyr: 3.736 ± 0.336
0.0GlyXaa: 0.0 ± 0.0
His
0.918HisAla: 0.918 ± 0.164
0.128HisCys: 0.128 ± 0.057
0.79HisAsp: 0.79 ± 0.128
1.025HisGlu: 1.025 ± 0.166
0.918HisPhe: 0.918 ± 0.148
1.26HisGly: 1.26 ± 0.161
0.512HisHis: 0.512 ± 0.131
1.494HisIle: 1.494 ± 0.234
1.601HisLys: 1.601 ± 0.19
1.793HisLeu: 1.793 ± 0.234
0.491HisMet: 0.491 ± 0.114
1.067HisAsn: 1.067 ± 0.155
0.512HisPro: 0.512 ± 0.094
0.47HisGln: 0.47 ± 0.099
1.046HisArg: 1.046 ± 0.153
0.875HisSer: 0.875 ± 0.121
1.26HisThr: 1.26 ± 0.16
1.238HisVal: 1.238 ± 0.189
0.192HisTrp: 0.192 ± 0.062
0.705HisTyr: 0.705 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
5.081IleAla: 5.081 ± 0.372
0.555IleCys: 0.555 ± 0.105
4.974IleAsp: 4.974 ± 0.369
5.145IleGlu: 5.145 ± 0.363
2.391IlePhe: 2.391 ± 0.275
3.736IleGly: 3.736 ± 0.337
1.025IleHis: 1.025 ± 0.181
4.035IleIle: 4.035 ± 0.341
4.718IleLys: 4.718 ± 0.34
4.313IleLeu: 4.313 ± 0.296
1.43IleMet: 1.43 ± 0.17
3.694IleAsn: 3.694 ± 0.298
2.498IlePro: 2.498 ± 0.24
2.327IleGln: 2.327 ± 0.211
2.775IleArg: 2.775 ± 0.263
4.035IleSer: 4.035 ± 0.274
4.547IleThr: 4.547 ± 0.41
4.398IleVal: 4.398 ± 0.342
0.427IleTrp: 0.427 ± 0.085
2.413IleTyr: 2.413 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
5.7LysAla: 5.7 ± 0.352
0.363LysCys: 0.363 ± 0.115
4.313LysAsp: 4.313 ± 0.289
6.682LysGlu: 6.682 ± 0.479
2.583LysPhe: 2.583 ± 0.25
5.103LysGly: 5.103 ± 0.416
1.473LysHis: 1.473 ± 0.218
4.014LysIle: 4.014 ± 0.258
5.807LysLys: 5.807 ± 0.458
6.106LysLeu: 6.106 ± 0.366
2.135LysMet: 2.135 ± 0.218
3.373LysAsn: 3.373 ± 0.271
2.861LysPro: 2.861 ± 0.309
3.117LysGln: 3.117 ± 0.256
3.587LysArg: 3.587 ± 0.326
4.462LysSer: 4.462 ± 0.371
3.672LysThr: 3.672 ± 0.289
5.337LysVal: 5.337 ± 0.333
0.576LysTrp: 0.576 ± 0.107
2.818LysTyr: 2.818 ± 0.21
0.0LysXaa: 0.0 ± 0.0
Leu
5.658LeuAla: 5.658 ± 0.273
0.534LeuCys: 0.534 ± 0.116
5.231LeuAsp: 5.231 ± 0.369
6.682LeuGlu: 6.682 ± 0.434
3.01LeuPhe: 3.01 ± 0.272
4.974LeuGly: 4.974 ± 0.364
1.409LeuHis: 1.409 ± 0.182
5.081LeuIle: 5.081 ± 0.398
5.679LeuLys: 5.679 ± 0.402
5.551LeuLeu: 5.551 ± 0.416
2.071LeuMet: 2.071 ± 0.26
4.59LeuAsn: 4.59 ± 0.314
3.16LeuPro: 3.16 ± 0.27
3.758LeuGln: 3.758 ± 0.329
3.587LeuArg: 3.587 ± 0.268
5.06LeuSer: 5.06 ± 0.333
5.06LeuThr: 5.06 ± 0.375
4.547LeuVal: 4.547 ± 0.282
0.833LeuTrp: 0.833 ± 0.128
3.16LeuTyr: 3.16 ± 0.232
0.0LeuXaa: 0.0 ± 0.0
Met
2.327MetAla: 2.327 ± 0.192
0.192MetCys: 0.192 ± 0.069
1.751MetAsp: 1.751 ± 0.188
2.092MetGlu: 2.092 ± 0.215
1.067MetPhe: 1.067 ± 0.15
1.302MetGly: 1.302 ± 0.19
0.406MetHis: 0.406 ± 0.102
1.58MetIle: 1.58 ± 0.188
2.05MetLys: 2.05 ± 0.208
2.178MetLeu: 2.178 ± 0.211
0.662MetMet: 0.662 ± 0.113
1.665MetAsn: 1.665 ± 0.178
0.854MetPro: 0.854 ± 0.157
1.046MetGln: 1.046 ± 0.164
1.132MetArg: 1.132 ± 0.154
1.857MetSer: 1.857 ± 0.203
1.751MetThr: 1.751 ± 0.192
1.601MetVal: 1.601 ± 0.207
0.32MetTrp: 0.32 ± 0.087
1.196MetTyr: 1.196 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.565AsnAla: 3.565 ± 0.255
0.363AsnCys: 0.363 ± 0.094
3.032AsnAsp: 3.032 ± 0.224
3.587AsnGlu: 3.587 ± 0.251
2.135AsnPhe: 2.135 ± 0.219
4.334AsnGly: 4.334 ± 0.361
1.174AsnHis: 1.174 ± 0.152
3.608AsnIle: 3.608 ± 0.29
4.035AsnLys: 4.035 ± 0.284
4.12AsnLeu: 4.12 ± 0.276
1.623AsnMet: 1.623 ± 0.186
3.416AsnAsn: 3.416 ± 0.373
2.391AsnPro: 2.391 ± 0.267
1.836AsnGln: 1.836 ± 0.224
2.348AsnArg: 2.348 ± 0.236
3.352AsnSer: 3.352 ± 0.315
3.822AsnThr: 3.822 ± 0.28
3.715AsnVal: 3.715 ± 0.265
0.512AsnTrp: 0.512 ± 0.101
2.092AsnTyr: 2.092 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
2.455ProAla: 2.455 ± 0.286
0.149ProCys: 0.149 ± 0.062
2.22ProAsp: 2.22 ± 0.225
2.861ProGlu: 2.861 ± 0.332
1.11ProPhe: 1.11 ± 0.145
1.132ProGly: 1.132 ± 0.15
0.683ProHis: 0.683 ± 0.118
2.178ProIle: 2.178 ± 0.243
2.199ProLys: 2.199 ± 0.246
2.605ProLeu: 2.605 ± 0.237
0.726ProMet: 0.726 ± 0.127
1.751ProAsn: 1.751 ± 0.219
0.726ProPro: 0.726 ± 0.129
1.409ProGln: 1.409 ± 0.195
1.174ProArg: 1.174 ± 0.151
2.05ProSer: 2.05 ± 0.249
2.904ProThr: 2.904 ± 0.3
2.797ProVal: 2.797 ± 0.311
0.171ProTrp: 0.171 ± 0.064
1.366ProTyr: 1.366 ± 0.144
0.0ProXaa: 0.0 ± 0.0
Gln
3.074GlnAla: 3.074 ± 0.33
0.192GlnCys: 0.192 ± 0.055
2.05GlnAsp: 2.05 ± 0.268
3.267GlnGlu: 3.267 ± 0.311
1.623GlnPhe: 1.623 ± 0.189
2.22GlnGly: 2.22 ± 0.235
0.961GlnHis: 0.961 ± 0.175
1.729GlnIle: 1.729 ± 0.206
2.306GlnLys: 2.306 ± 0.229
3.565GlnLeu: 3.565 ± 0.288
1.302GlnMet: 1.302 ± 0.191
1.964GlnAsn: 1.964 ± 0.22
1.217GlnPro: 1.217 ± 0.302
1.644GlnGln: 1.644 ± 0.228
1.473GlnArg: 1.473 ± 0.163
2.306GlnSer: 2.306 ± 0.251
2.306GlnThr: 2.306 ± 0.237
2.498GlnVal: 2.498 ± 0.213
0.491GlnTrp: 0.491 ± 0.115
1.943GlnTyr: 1.943 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
2.797ArgAla: 2.797 ± 0.281
0.235ArgCys: 0.235 ± 0.075
2.69ArgAsp: 2.69 ± 0.235
3.16ArgGlu: 3.16 ± 0.244
1.943ArgPhe: 1.943 ± 0.196
2.861ArgGly: 2.861 ± 0.293
0.726ArgHis: 0.726 ± 0.121
3.01ArgIle: 3.01 ± 0.215
3.48ArgLys: 3.48 ± 0.272
3.971ArgLeu: 3.971 ± 0.305
1.452ArgMet: 1.452 ± 0.166
2.263ArgAsn: 2.263 ± 0.25
1.43ArgPro: 1.43 ± 0.193
1.559ArgGln: 1.559 ± 0.179
1.452ArgArg: 1.452 ± 0.174
1.986ArgSer: 1.986 ± 0.226
2.37ArgThr: 2.37 ± 0.222
3.01ArgVal: 3.01 ± 0.308
0.406ArgTrp: 0.406 ± 0.088
2.007ArgTyr: 2.007 ± 0.198
0.0ArgXaa: 0.0 ± 0.0
Ser
3.331SerAla: 3.331 ± 0.288
0.384SerCys: 0.384 ± 0.106
3.267SerAsp: 3.267 ± 0.278
4.078SerGlu: 4.078 ± 0.319
2.797SerPhe: 2.797 ± 0.265
4.59SerGly: 4.59 ± 0.421
0.747SerHis: 0.747 ± 0.127
3.928SerIle: 3.928 ± 0.273
4.355SerLys: 4.355 ± 0.34
4.505SerLeu: 4.505 ± 0.317
1.345SerMet: 1.345 ± 0.176
3.331SerAsn: 3.331 ± 0.321
1.793SerPro: 1.793 ± 0.255
1.708SerGln: 1.708 ± 0.198
2.647SerArg: 2.647 ± 0.269
4.163SerSer: 4.163 ± 0.38
3.651SerThr: 3.651 ± 0.298
3.928SerVal: 3.928 ± 0.367
0.598SerTrp: 0.598 ± 0.103
3.074SerTyr: 3.074 ± 0.253
0.0SerXaa: 0.0 ± 0.0
Thr
4.633ThrAla: 4.633 ± 0.431
0.278ThrCys: 0.278 ± 0.079
3.886ThrAsp: 3.886 ± 0.34
4.291ThrGlu: 4.291 ± 0.351
2.519ThrPhe: 2.519 ± 0.221
4.868ThrGly: 4.868 ± 0.425
1.067ThrHis: 1.067 ± 0.133
4.398ThrIle: 4.398 ± 0.346
4.313ThrLys: 4.313 ± 0.323
4.974ThrLeu: 4.974 ± 0.397
1.067ThrMet: 1.067 ± 0.149
3.822ThrAsn: 3.822 ± 0.319
2.626ThrPro: 2.626 ± 0.294
2.434ThrGln: 2.434 ± 0.212
2.519ThrArg: 2.519 ± 0.261
3.245ThrSer: 3.245 ± 0.328
4.526ThrThr: 4.526 ± 0.444
5.722ThrVal: 5.722 ± 0.404
0.705ThrTrp: 0.705 ± 0.131
3.309ThrTyr: 3.309 ± 0.344
0.0ThrXaa: 0.0 ± 0.0
Val
4.612ValAla: 4.612 ± 0.306
0.448ValCys: 0.448 ± 0.099
4.761ValAsp: 4.761 ± 0.383
4.996ValGlu: 4.996 ± 0.359
2.861ValPhe: 2.861 ± 0.258
3.8ValGly: 3.8 ± 0.323
1.452ValHis: 1.452 ± 0.186
3.715ValIle: 3.715 ± 0.307
5.273ValLys: 5.273 ± 0.357
4.868ValLeu: 4.868 ± 0.358
1.729ValMet: 1.729 ± 0.178
3.736ValAsn: 3.736 ± 0.299
2.541ValPro: 2.541 ± 0.286
2.989ValGln: 2.989 ± 0.298
3.416ValArg: 3.416 ± 0.3
3.864ValSer: 3.864 ± 0.256
5.039ValThr: 5.039 ± 0.349
3.992ValVal: 3.992 ± 0.33
0.491ValTrp: 0.491 ± 0.085
3.096ValTyr: 3.096 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
0.79TrpAla: 0.79 ± 0.144
0.064TrpCys: 0.064 ± 0.04
0.897TrpAsp: 0.897 ± 0.135
0.833TrpGlu: 0.833 ± 0.15
0.491TrpPhe: 0.491 ± 0.134
0.619TrpGly: 0.619 ± 0.117
0.149TrpHis: 0.149 ± 0.054
0.342TrpIle: 0.342 ± 0.083
0.79TrpLys: 0.79 ± 0.115
0.747TrpLeu: 0.747 ± 0.128
0.192TrpMet: 0.192 ± 0.055
0.448TrpAsn: 0.448 ± 0.096
0.085TrpPro: 0.085 ± 0.041
0.406TrpGln: 0.406 ± 0.092
0.213TrpArg: 0.213 ± 0.058
0.598TrpSer: 0.598 ± 0.111
0.811TrpThr: 0.811 ± 0.144
0.769TrpVal: 0.769 ± 0.123
0.107TrpTrp: 0.107 ± 0.05
0.619TrpTyr: 0.619 ± 0.098
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.818TyrAla: 2.818 ± 0.257
0.448TyrCys: 0.448 ± 0.093
2.754TyrAsp: 2.754 ± 0.248
3.117TyrGlu: 3.117 ± 0.294
1.644TyrPhe: 1.644 ± 0.204
2.904TyrGly: 2.904 ± 0.294
0.811TyrHis: 0.811 ± 0.161
3.288TyrIle: 3.288 ± 0.281
3.352TyrLys: 3.352 ± 0.305
3.843TyrLeu: 3.843 ± 0.298
1.153TyrMet: 1.153 ± 0.154
2.925TyrAsn: 2.925 ± 0.227
1.217TyrPro: 1.217 ± 0.164
1.623TyrGln: 1.623 ± 0.158
2.263TyrArg: 2.263 ± 0.226
2.861TyrSer: 2.861 ± 0.261
2.882TyrThr: 2.882 ± 0.296
2.754TyrVal: 2.754 ± 0.245
0.278TyrTrp: 0.278 ± 0.073
1.665TyrTyr: 1.665 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 222 proteins (46840 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski