Stagonospora sp. SRC1lsM3a

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Massarineae; Massarinaceae; Stagonospora; unclassified Stagonospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A178ASY3|A0A178ASY3_9PLEO Histone-fold-containing protein OS=Stagonospora sp. SRC1lsM3a OX=765868 GN=IQ06DRAFT_336281 PE=4 SV=1
MM1 pKa = 7.74DD2 pKa = 4.09TLEE5 pKa = 4.19RR6 pKa = 11.84QQLRR10 pKa = 11.84LTDD13 pKa = 3.81TEE15 pKa = 4.21DD16 pKa = 3.22TGPVLSFVDD25 pKa = 4.76FEE27 pKa = 4.68VGDD30 pKa = 5.04DD31 pKa = 4.39INDD34 pKa = 3.51TSIGYY39 pKa = 9.22EE40 pKa = 3.93GYY42 pKa = 11.03NYY44 pKa = 10.4VEE46 pKa = 4.31VSAVQSVLFEE56 pKa = 4.74LNYY59 pKa = 9.44PDD61 pKa = 4.54PYY63 pKa = 11.36GPISGKK69 pKa = 7.5TPRR72 pKa = 11.84LLPLGYY78 pKa = 10.15SITEE82 pKa = 4.19GVQDD86 pKa = 3.68SSGNGYY92 pKa = 9.83RR93 pKa = 11.84DD94 pKa = 2.91GRR96 pKa = 11.84ADD98 pKa = 3.72YY99 pKa = 10.87LVGAMTTVVV108 pKa = 3.09

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A178AWR1|A0A178AWR1_9PLEO Uncharacterized protein OS=Stagonospora sp. SRC1lsM3a OX=765868 GN=IQ06DRAFT_368481 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.43VSIIALFAAGAIAAPLAAPQAPSGTPVVPGFPGGAPSGGPGGFPGGFPSGFPAPTGGFPGFPSGGFPAPTGGFPRR77 pKa = 11.84PTGPRR82 pKa = 11.84PSGGPGGSRR91 pKa = 11.84PTGGPRR97 pKa = 11.84PTGGPRR103 pKa = 11.84PSGAPPVFSGGPAVPAPTITT123 pKa = 3.41

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14420

0

14420

6584226

49

7200

456.6

50.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.801 ± 0.018

1.327 ± 0.009

5.673 ± 0.015

5.998 ± 0.02

3.739 ± 0.013

6.784 ± 0.02

2.493 ± 0.01

4.92 ± 0.014

4.936 ± 0.017

8.715 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.007

3.711 ± 0.012

5.901 ± 0.024

4.053 ± 0.016

5.995 ± 0.017

8.08 ± 0.024

6.066 ± 0.014

6.118 ± 0.016

1.561 ± 0.008

2.866 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski