Amino acid dipepetide frequency for Stagonospora sp. SRC1lsM3a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.995AlaAla: 8.995 ± 0.051
1.171AlaCys: 1.171 ± 0.014
4.28AlaAsp: 4.28 ± 0.025
5.022AlaGlu: 5.022 ± 0.031
3.28AlaPhe: 3.28 ± 0.027
5.832AlaGly: 5.832 ± 0.042
1.967AlaHis: 1.967 ± 0.018
4.406AlaIle: 4.406 ± 0.029
4.299AlaLys: 4.299 ± 0.028
7.85AlaLeu: 7.85 ± 0.04
2.096AlaMet: 2.096 ± 0.018
3.161AlaAsn: 3.161 ± 0.024
5.098AlaPro: 5.098 ± 0.043
3.703AlaGln: 3.703 ± 0.029
4.993AlaArg: 4.993 ± 0.03
7.398AlaSer: 7.398 ± 0.042
5.475AlaThr: 5.475 ± 0.034
5.332AlaVal: 5.332 ± 0.034
1.286AlaTrp: 1.286 ± 0.015
2.369AlaTyr: 2.369 ± 0.022
0.0AlaXaa: 0.0 ± 0.0
Cys
1.079CysAla: 1.079 ± 0.013
0.288CysCys: 0.288 ± 0.009
0.713CysAsp: 0.713 ± 0.012
0.625CysGlu: 0.625 ± 0.01
0.555CysPhe: 0.555 ± 0.012
1.011CysGly: 1.011 ± 0.013
0.345CysHis: 0.345 ± 0.008
0.797CysIle: 0.797 ± 0.01
0.579CysLys: 0.579 ± 0.01
1.237CysLeu: 1.237 ± 0.016
0.315CysMet: 0.315 ± 0.007
0.496CysAsn: 0.496 ± 0.009
0.682CysPro: 0.682 ± 0.01
0.456CysGln: 0.456 ± 0.009
0.808CysArg: 0.808 ± 0.013
1.001CysSer: 1.001 ± 0.013
0.806CysThr: 0.806 ± 0.013
0.849CysVal: 0.849 ± 0.011
0.228CysTrp: 0.228 ± 0.006
0.399CysTyr: 0.399 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
5.05AspAla: 5.05 ± 0.028
0.644AspCys: 0.644 ± 0.01
4.179AspAsp: 4.179 ± 0.037
4.325AspGlu: 4.325 ± 0.032
2.286AspPhe: 2.286 ± 0.02
3.939AspGly: 3.939 ± 0.027
1.275AspHis: 1.275 ± 0.015
3.056AspIle: 3.056 ± 0.023
2.452AspLys: 2.452 ± 0.02
4.915AspLeu: 4.915 ± 0.028
1.374AspMet: 1.374 ± 0.014
1.862AspAsn: 1.862 ± 0.02
3.128AspPro: 3.128 ± 0.024
1.818AspGln: 1.818 ± 0.018
2.982AspArg: 2.982 ± 0.027
3.922AspSer: 3.922 ± 0.029
3.07AspThr: 3.07 ± 0.023
3.853AspVal: 3.853 ± 0.023
0.97AspTrp: 0.97 ± 0.012
1.632AspTyr: 1.632 ± 0.017
0.0AspXaa: 0.0 ± 0.0
Glu
5.249GluAla: 5.249 ± 0.037
0.679GluCys: 0.679 ± 0.011
4.175GluAsp: 4.175 ± 0.029
5.161GluGlu: 5.161 ± 0.043
1.838GluPhe: 1.838 ± 0.017
3.747GluGly: 3.747 ± 0.026
1.557GluHis: 1.557 ± 0.015
2.834GluIle: 2.834 ± 0.022
3.696GluLys: 3.696 ± 0.03
5.032GluLeu: 5.032 ± 0.033
1.451GluMet: 1.451 ± 0.015
2.103GluAsn: 2.103 ± 0.017
2.613GluPro: 2.613 ± 0.027
2.592GluGln: 2.592 ± 0.02
3.874GluArg: 3.874 ± 0.031
3.963GluSer: 3.963 ± 0.026
3.223GluThr: 3.223 ± 0.026
3.557GluVal: 3.557 ± 0.026
0.945GluTrp: 0.945 ± 0.013
1.688GluTyr: 1.688 ± 0.016
0.0GluXaa: 0.0 ± 0.0
Phe
3.312PheAla: 3.312 ± 0.026
0.573PheCys: 0.573 ± 0.009
2.347PheAsp: 2.347 ± 0.018
2.198PheGlu: 2.198 ± 0.019
1.6PhePhe: 1.6 ± 0.02
2.913PheGly: 2.913 ± 0.026
0.883PheHis: 0.883 ± 0.012
1.755PheIle: 1.755 ± 0.02
1.565PheLys: 1.565 ± 0.016
3.359PheLeu: 3.359 ± 0.028
0.805PheMet: 0.805 ± 0.011
1.44PheAsn: 1.44 ± 0.018
1.919PhePro: 1.919 ± 0.019
1.376PheGln: 1.376 ± 0.014
1.95PheArg: 1.95 ± 0.018
2.938PheSer: 2.938 ± 0.024
2.226PheThr: 2.226 ± 0.018
2.432PheVal: 2.432 ± 0.021
0.689PheTrp: 0.689 ± 0.011
1.109PheTyr: 1.109 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
5.481GlyAla: 5.481 ± 0.037
0.948GlyCys: 0.948 ± 0.013
3.526GlyAsp: 3.526 ± 0.024
3.576GlyGlu: 3.576 ± 0.026
2.766GlyPhe: 2.766 ± 0.023
5.685GlyGly: 5.685 ± 0.055
1.702GlyHis: 1.702 ± 0.018
3.543GlyIle: 3.543 ± 0.029
3.572GlyLys: 3.572 ± 0.027
5.852GlyLeu: 5.852 ± 0.038
1.714GlyMet: 1.714 ± 0.02
2.531GlyAsn: 2.531 ± 0.022
3.181GlyPro: 3.181 ± 0.028
2.528GlyGln: 2.528 ± 0.024
3.97GlyArg: 3.97 ± 0.027
5.483GlySer: 5.483 ± 0.035
4.033GlyThr: 4.033 ± 0.03
4.347GlyVal: 4.347 ± 0.03
1.237GlyTrp: 1.237 ± 0.014
2.165GlyTyr: 2.165 ± 0.024
0.0GlyXaa: 0.0 ± 0.0
His
2.037HisAla: 2.037 ± 0.017
0.375HisCys: 0.375 ± 0.008
1.461HisAsp: 1.461 ± 0.016
1.395HisGlu: 1.395 ± 0.016
0.992HisPhe: 0.992 ± 0.013
1.769HisGly: 1.769 ± 0.017
0.867HisHis: 0.867 ± 0.016
1.29HisIle: 1.29 ± 0.014
0.991HisLys: 0.991 ± 0.012
2.211HisLeu: 2.211 ± 0.018
0.546HisMet: 0.546 ± 0.009
0.921HisAsn: 0.921 ± 0.012
1.608HisPro: 1.608 ± 0.017
0.981HisGln: 0.981 ± 0.013
1.523HisArg: 1.523 ± 0.017
1.873HisSer: 1.873 ± 0.019
1.417HisThr: 1.417 ± 0.015
1.582HisVal: 1.582 ± 0.017
0.376HisTrp: 0.376 ± 0.007
0.714HisTyr: 0.714 ± 0.011
0.0HisXaa: 0.0 ± 0.0
Ile
4.506IleAla: 4.506 ± 0.031
0.797IleCys: 0.797 ± 0.012
2.939IleAsp: 2.939 ± 0.022
2.924IleGlu: 2.924 ± 0.021
1.997IlePhe: 1.997 ± 0.02
3.264IleGly: 3.264 ± 0.03
1.184IleHis: 1.184 ± 0.013
2.484IleIle: 2.484 ± 0.024
2.176IleLys: 2.176 ± 0.021
4.401IleLeu: 4.401 ± 0.036
1.075IleMet: 1.075 ± 0.012
1.808IleAsn: 1.808 ± 0.016
2.917IlePro: 2.917 ± 0.022
1.822IleGln: 1.822 ± 0.018
2.681IleArg: 2.681 ± 0.02
3.724IleSer: 3.724 ± 0.028
2.94IleThr: 2.94 ± 0.023
3.317IleVal: 3.317 ± 0.025
0.779IleTrp: 0.779 ± 0.013
1.465IleTyr: 1.465 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
4.342LysAla: 4.342 ± 0.03
0.557LysCys: 0.557 ± 0.01
2.977LysAsp: 2.977 ± 0.026
3.434LysGlu: 3.434 ± 0.031
1.487LysPhe: 1.487 ± 0.015
3.125LysGly: 3.125 ± 0.025
1.238LysHis: 1.238 ± 0.014
2.286LysIle: 2.286 ± 0.02
3.478LysLys: 3.478 ± 0.041
4.042LysLeu: 4.042 ± 0.024
1.063LysMet: 1.063 ± 0.013
1.803LysAsn: 1.803 ± 0.017
2.708LysPro: 2.708 ± 0.026
1.927LysGln: 1.927 ± 0.02
3.421LysArg: 3.421 ± 0.03
3.552LysSer: 3.552 ± 0.026
2.876LysThr: 2.876 ± 0.024
2.897LysVal: 2.897 ± 0.024
0.731LysTrp: 0.731 ± 0.011
1.419LysTyr: 1.419 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
7.727LeuAla: 7.727 ± 0.039
1.273LeuCys: 1.273 ± 0.016
5.072LeuAsp: 5.072 ± 0.032
5.246LeuGlu: 5.246 ± 0.035
3.255LeuPhe: 3.255 ± 0.027
5.764LeuGly: 5.764 ± 0.034
2.293LeuHis: 2.293 ± 0.018
3.847LeuIle: 3.847 ± 0.025
4.169LeuLys: 4.169 ± 0.028
8.069LeuLeu: 8.069 ± 0.042
1.775LeuMet: 1.775 ± 0.018
3.154LeuAsn: 3.154 ± 0.022
5.427LeuPro: 5.427 ± 0.035
3.851LeuGln: 3.851 ± 0.027
5.581LeuArg: 5.581 ± 0.037
7.007LeuSer: 7.007 ± 0.044
4.71LeuThr: 4.71 ± 0.026
5.306LeuVal: 5.306 ± 0.032
1.255LeuTrp: 1.255 ± 0.015
2.374LeuTyr: 2.374 ± 0.02
0.0LeuXaa: 0.0 ± 0.0
Met
2.218MetAla: 2.218 ± 0.022
0.28MetCys: 0.28 ± 0.006
1.234MetAsp: 1.234 ± 0.015
1.252MetGlu: 1.252 ± 0.014
0.816MetPhe: 0.816 ± 0.012
1.491MetGly: 1.491 ± 0.016
0.558MetHis: 0.558 ± 0.009
1.01MetIle: 1.01 ± 0.014
1.083MetLys: 1.083 ± 0.012
1.96MetLeu: 1.96 ± 0.018
0.61MetMet: 0.61 ± 0.011
0.822MetAsn: 0.822 ± 0.012
1.356MetPro: 1.356 ± 0.015
0.958MetGln: 0.958 ± 0.012
1.381MetArg: 1.381 ± 0.014
2.023MetSer: 2.023 ± 0.018
1.314MetThr: 1.314 ± 0.015
1.328MetVal: 1.328 ± 0.015
0.304MetTrp: 0.304 ± 0.007
0.62MetTyr: 0.62 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
3.396AsnAla: 3.396 ± 0.024
0.474AsnCys: 0.474 ± 0.008
2.1AsnAsp: 2.1 ± 0.019
2.032AsnGlu: 2.032 ± 0.019
1.42AsnPhe: 1.42 ± 0.015
3.017AsnGly: 3.017 ± 0.025
0.853AsnHis: 0.853 ± 0.011
2.082AsnIle: 2.082 ± 0.018
1.625AsnLys: 1.625 ± 0.016
3.149AsnLeu: 3.149 ± 0.025
0.918AsnMet: 0.918 ± 0.01
1.511AsnAsn: 1.511 ± 0.017
2.299AsnPro: 2.299 ± 0.017
1.294AsnGln: 1.294 ± 0.016
1.849AsnArg: 1.849 ± 0.019
2.665AsnSer: 2.665 ± 0.023
2.318AsnThr: 2.318 ± 0.02
2.447AsnVal: 2.447 ± 0.018
0.588AsnTrp: 0.588 ± 0.011
1.075AsnTyr: 1.075 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
5.319ProAla: 5.319 ± 0.04
0.543ProCys: 0.543 ± 0.01
3.014ProAsp: 3.014 ± 0.021
3.511ProGlu: 3.511 ± 0.028
2.042ProPhe: 2.042 ± 0.02
3.687ProGly: 3.687 ± 0.03
1.404ProHis: 1.404 ± 0.018
2.552ProIle: 2.552 ± 0.019
2.725ProLys: 2.725 ± 0.026
4.598ProLeu: 4.598 ± 0.03
1.111ProMet: 1.111 ± 0.013
2.229ProAsn: 2.229 ± 0.021
4.833ProPro: 4.833 ± 0.06
2.5ProGln: 2.5 ± 0.028
3.297ProArg: 3.297 ± 0.026
5.778ProSer: 5.778 ± 0.044
4.106ProThr: 4.106 ± 0.033
3.406ProVal: 3.406 ± 0.027
0.773ProTrp: 0.773 ± 0.013
1.579ProTyr: 1.579 ± 0.016
0.0ProXaa: 0.0 ± 0.0
Gln
3.548GlnAla: 3.548 ± 0.027
0.517GlnCys: 0.517 ± 0.008
2.166GlnAsp: 2.166 ± 0.021
2.366GlnGlu: 2.366 ± 0.02
1.301GlnPhe: 1.301 ± 0.015
2.402GlnGly: 2.402 ± 0.021
1.206GlnHis: 1.206 ± 0.014
1.919GlnIle: 1.919 ± 0.017
1.972GlnLys: 1.972 ± 0.018
3.433GlnLeu: 3.433 ± 0.027
0.898GlnMet: 0.898 ± 0.013
1.612GlnAsn: 1.612 ± 0.017
2.518GlnPro: 2.518 ± 0.024
2.536GlnGln: 2.536 ± 0.049
2.687GlnArg: 2.687 ± 0.023
3.145GlnSer: 3.145 ± 0.025
2.302GlnThr: 2.302 ± 0.019
2.135GlnVal: 2.135 ± 0.02
0.606GlnTrp: 0.606 ± 0.008
1.26GlnTyr: 1.26 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
4.728ArgAla: 4.728 ± 0.031
0.794ArgCys: 0.794 ± 0.012
3.29ArgAsp: 3.29 ± 0.028
3.598ArgGlu: 3.598 ± 0.027
2.096ArgPhe: 2.096 ± 0.017
3.63ArgGly: 3.63 ± 0.03
1.577ArgHis: 1.577 ± 0.017
2.932ArgIle: 2.932 ± 0.023
3.444ArgLys: 3.444 ± 0.026
5.23ArgLeu: 5.23 ± 0.032
1.398ArgMet: 1.398 ± 0.016
2.346ArgAsn: 2.346 ± 0.019
3.406ArgPro: 3.406 ± 0.026
2.546ArgGln: 2.546 ± 0.02
4.845ArgArg: 4.845 ± 0.043
4.694ArgSer: 4.694 ± 0.036
3.434ArgThr: 3.434 ± 0.026
3.281ArgVal: 3.281 ± 0.022
0.979ArgTrp: 0.979 ± 0.012
1.701ArgTyr: 1.701 ± 0.015
0.0ArgXaa: 0.0 ± 0.0
Ser
6.781SerAla: 6.781 ± 0.038
0.95SerCys: 0.95 ± 0.012
4.149SerAsp: 4.149 ± 0.033
3.934SerGlu: 3.934 ± 0.028
2.989SerPhe: 2.989 ± 0.02
5.317SerGly: 5.317 ± 0.037
1.991SerHis: 1.991 ± 0.02
4.041SerIle: 4.041 ± 0.026
3.783SerLys: 3.783 ± 0.026
6.882SerLeu: 6.882 ± 0.038
1.768SerMet: 1.768 ± 0.018
3.082SerAsn: 3.082 ± 0.027
5.208SerPro: 5.208 ± 0.044
3.158SerGln: 3.158 ± 0.028
4.856SerArg: 4.856 ± 0.037
8.408SerSer: 8.408 ± 0.07
5.702SerThr: 5.702 ± 0.038
4.458SerVal: 4.458 ± 0.026
1.193SerTrp: 1.193 ± 0.015
2.155SerTyr: 2.155 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
5.284ThrAla: 5.284 ± 0.028
0.876ThrCys: 0.876 ± 0.014
2.832ThrAsp: 2.832 ± 0.02
2.988ThrGlu: 2.988 ± 0.022
2.416ThrPhe: 2.416 ± 0.022
4.021ThrGly: 4.021 ± 0.028
1.386ThrHis: 1.386 ± 0.015
3.227ThrIle: 3.227 ± 0.025
2.749ThrLys: 2.749 ± 0.02
5.314ThrLeu: 5.314 ± 0.035
1.263ThrMet: 1.263 ± 0.014
2.203ThrAsn: 2.203 ± 0.021
4.353ThrPro: 4.353 ± 0.036
2.214ThrGln: 2.214 ± 0.019
3.165ThrArg: 3.165 ± 0.024
5.522ThrSer: 5.522 ± 0.036
4.464ThrThr: 4.464 ± 0.042
3.673ThrVal: 3.673 ± 0.028
0.968ThrTrp: 0.968 ± 0.012
1.742ThrTyr: 1.742 ± 0.017
0.0ThrXaa: 0.0 ± 0.0
Val
5.414ValAla: 5.414 ± 0.031
0.844ValCys: 0.844 ± 0.013
3.629ValAsp: 3.629 ± 0.024
3.903ValGlu: 3.903 ± 0.026
2.444ValPhe: 2.444 ± 0.024
4.083ValGly: 4.083 ± 0.029
1.466ValHis: 1.466 ± 0.017
2.851ValIle: 2.851 ± 0.028
2.975ValLys: 2.975 ± 0.027
5.546ValLeu: 5.546 ± 0.03
1.354ValMet: 1.354 ± 0.016
2.185ValAsn: 2.185 ± 0.019
3.578ValPro: 3.578 ± 0.029
2.501ValGln: 2.501 ± 0.022
3.458ValArg: 3.458 ± 0.024
4.461ValSer: 4.461 ± 0.027
3.482ValThr: 3.482 ± 0.028
4.314ValVal: 4.314 ± 0.03
0.934ValTrp: 0.934 ± 0.012
1.754ValTyr: 1.754 ± 0.018
0.0ValXaa: 0.0 ± 0.0
Trp
1.199TrpAla: 1.199 ± 0.012
0.231TrpCys: 0.231 ± 0.006
0.927TrpAsp: 0.927 ± 0.014
0.869TrpGlu: 0.869 ± 0.011
0.569TrpPhe: 0.569 ± 0.009
1.011TrpGly: 1.011 ± 0.014
0.392TrpHis: 0.392 ± 0.009
0.846TrpIle: 0.846 ± 0.012
0.845TrpLys: 0.845 ± 0.01
1.419TrpLeu: 1.419 ± 0.015
0.403TrpMet: 0.403 ± 0.008
0.675TrpAsn: 0.675 ± 0.011
0.656TrpPro: 0.656 ± 0.011
0.636TrpGln: 0.636 ± 0.01
1.021TrpArg: 1.021 ± 0.014
1.166TrpSer: 1.166 ± 0.013
1.012TrpThr: 1.012 ± 0.013
0.947TrpVal: 0.947 ± 0.013
0.295TrpTrp: 0.295 ± 0.007
0.493TrpTyr: 0.493 ± 0.009
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.348TyrAla: 2.348 ± 0.02
0.454TyrCys: 0.454 ± 0.009
1.722TyrAsp: 1.722 ± 0.018
1.617TyrGlu: 1.617 ± 0.016
1.244TyrPhe: 1.244 ± 0.014
2.131TyrGly: 2.131 ± 0.023
0.784TyrHis: 0.784 ± 0.012
1.443TyrIle: 1.443 ± 0.015
1.187TyrLys: 1.187 ± 0.013
2.655TyrLeu: 2.655 ± 0.02
0.684TyrMet: 0.684 ± 0.01
1.164TyrAsn: 1.164 ± 0.014
1.519TyrPro: 1.519 ± 0.02
1.13TyrGln: 1.13 ± 0.015
1.606TyrArg: 1.606 ± 0.019
2.081TyrSer: 2.081 ± 0.02
1.75TyrThr: 1.75 ± 0.018
1.718TyrVal: 1.718 ± 0.017
0.478TyrTrp: 0.478 ± 0.01
0.949TyrTyr: 0.949 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 14420 proteins (6584226 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski