Salinicoccus alkaliphilus DSM 16010
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2512 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7HGP6|A0A1M7HGP6_9STAP Uncharacterized protein OS=Salinicoccus alkaliphilus DSM 16010 OX=1123231 GN=SAMN02745189_01859 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.2 KK3 pKa = 10.49 YY4 pKa = 10.75 LILVLSLVFVLAACTDD20 pKa = 3.74 DD21 pKa = 5.31 GEE23 pKa = 4.36 VDD25 pKa = 3.25 EE26 pKa = 4.88 ATEE29 pKa = 4.1 EE30 pKa = 4.21 EE31 pKa = 4.64 ATGEE35 pKa = 4.04 DD36 pKa = 3.67 AAEE39 pKa = 4.36 EE40 pKa = 4.8 GSTGGSEE47 pKa = 4.14 GTINMSLLATPNSLDD62 pKa = 3.18 PHH64 pKa = 6.75 AANDD68 pKa = 3.63 QPSNHH73 pKa = 5.51 VNVNIYY79 pKa = 9.9 EE80 pKa = 4.19 RR81 pKa = 11.84 LVEE84 pKa = 4.3 FTPEE88 pKa = 3.95 LEE90 pKa = 4.45 LEE92 pKa = 4.58 PGLAEE97 pKa = 4.44 SYY99 pKa = 10.5 EE100 pKa = 4.15 QVEE103 pKa = 4.35 DD104 pKa = 3.98 TTWEE108 pKa = 3.8 FSIRR112 pKa = 11.84 EE113 pKa = 4.16 GVSFHH118 pKa = 7.68 DD119 pKa = 4.24 GEE121 pKa = 4.34 EE122 pKa = 4.21 LNAEE126 pKa = 4.0 AVKK129 pKa = 11.03 ANLDD133 pKa = 3.53 RR134 pKa = 11.84 VTDD137 pKa = 3.83 EE138 pKa = 5.32 DD139 pKa = 3.79 VGSPVAFLFEE149 pKa = 5.19 LIEE152 pKa = 4.12 EE153 pKa = 4.45 VEE155 pKa = 4.51 VIDD158 pKa = 5.63 DD159 pKa = 4.04 YY160 pKa = 11.32 TVHH163 pKa = 6.38 IHH165 pKa = 5.78 TSSPFAALPSHH176 pKa = 6.99 LAHH179 pKa = 7.24 PAGGMISPAVIEE191 pKa = 3.89 EE192 pKa = 4.75 DD193 pKa = 3.84 YY194 pKa = 11.8 AEE196 pKa = 5.04 DD197 pKa = 3.71 EE198 pKa = 4.34 QLTAVHH204 pKa = 5.94 QNPVGTGAYY213 pKa = 8.83 QFEE216 pKa = 4.91 EE217 pKa = 4.42 ISEE220 pKa = 4.18 GDD222 pKa = 3.73 YY223 pKa = 10.0 VTLVKK228 pKa = 10.87 NEE230 pKa = 4.25 EE231 pKa = 4.22 YY232 pKa = 9.71 WGEE235 pKa = 3.93 EE236 pKa = 3.95 AEE238 pKa = 4.29 SDD240 pKa = 3.85 VFTFNAVPEE249 pKa = 4.44 DD250 pKa = 3.42 ATRR253 pKa = 11.84 IAEE256 pKa = 4.17 LQTRR260 pKa = 11.84 HH261 pKa = 6.38 ADD263 pKa = 3.99 LIFPLDD269 pKa = 4.02 PNDD272 pKa = 3.78 FEE274 pKa = 5.63 QINSDD279 pKa = 2.94 EE280 pKa = 4.17 GTTVNEE286 pKa = 4.3 TEE288 pKa = 4.51 SVRR291 pKa = 11.84 MEE293 pKa = 3.64 YY294 pKa = 10.69 VGFNVEE300 pKa = 4.59 VEE302 pKa = 4.31 PFDD305 pKa = 5.19 DD306 pKa = 3.96 PVVRR310 pKa = 11.84 QAIAHH315 pKa = 6.97 AIDD318 pKa = 4.37 KK319 pKa = 10.62 EE320 pKa = 4.32 DD321 pKa = 4.4 IINIMLEE328 pKa = 4.07 GKK330 pKa = 10.29 AVVADD335 pKa = 4.11 TLLSPAVFGHH345 pKa = 6.97 AEE347 pKa = 4.49 DD348 pKa = 4.19 IDD350 pKa = 4.83 GIGYY354 pKa = 9.75 DD355 pKa = 4.23 LEE357 pKa = 4.56 TAQEE361 pKa = 4.07 LLDD364 pKa = 4.31 DD365 pKa = 4.31 NGYY368 pKa = 10.76 EE369 pKa = 4.06 EE370 pKa = 5.3 GFSAEE375 pKa = 4.02 IVVQDD380 pKa = 3.88 RR381 pKa = 11.84 TAADD385 pKa = 2.99 IATYY389 pKa = 9.92 IQEE392 pKa = 4.21 QLQEE396 pKa = 4.19 LNIDD400 pKa = 3.72 LEE402 pKa = 4.74 IYY404 pKa = 10.56 QIDD407 pKa = 3.51 PGAYY411 pKa = 8.92 LDD413 pKa = 4.05 YY414 pKa = 10.83 VGSGSHH420 pKa = 7.19 DD421 pKa = 3.49 MFIGGWGTVTMDD433 pKa = 4.33 ADD435 pKa = 3.67 YY436 pKa = 11.45 GLYY439 pKa = 10.09 PLFHH443 pKa = 7.18 SSGIGNSGNRR453 pKa = 11.84 SRR455 pKa = 11.84 YY456 pKa = 9.66 ANEE459 pKa = 3.89 EE460 pKa = 3.78 VDD462 pKa = 4.05 TLLEE466 pKa = 4.17 DD467 pKa = 4.06 ARR469 pKa = 11.84 TEE471 pKa = 3.74 TDD473 pKa = 2.98 EE474 pKa = 4.21 AQRR477 pKa = 11.84 LALYY481 pKa = 10.36 EE482 pKa = 4.02 EE483 pKa = 4.31 AQEE486 pKa = 4.29 IIIAEE491 pKa = 4.01 APIIPIYY498 pKa = 10.39 HH499 pKa = 7.35 PYY501 pKa = 10.79 LLTGMSEE508 pKa = 4.95 DD509 pKa = 3.17 IDD511 pKa = 5.17 GYY513 pKa = 9.97 AQHH516 pKa = 7.11 PASFHH521 pKa = 5.79 FLSDD525 pKa = 3.43 VSKK528 pKa = 10.69 EE529 pKa = 3.94
Molecular weight: 58.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.605
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.63
Grimsley 3.516
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.656
Sillero 3.91
Patrickios 1.062
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A1M7GJP9|A0A1M7GJP9_9STAP Uncharacterized protein OS=Salinicoccus alkaliphilus DSM 16010 OX=1123231 GN=SAMN02745189_01663 PE=4 SV=1
MM1 pKa = 7.62 TKK3 pKa = 9.09 RR4 pKa = 11.84 TYY6 pKa = 10.29 QPNKK10 pKa = 8.16 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.87 VHH17 pKa = 5.77 GFRR20 pKa = 11.84 KK21 pKa = 10.02 RR22 pKa = 11.84 MSTKK26 pKa = 10.06 NGRR29 pKa = 11.84 KK30 pKa = 8.47 ILARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.09 GRR40 pKa = 11.84 KK41 pKa = 8.7 VLSAA45 pKa = 4.05
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2512
0
2512
753143
40
1488
299.8
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.3 ± 0.052
0.523 ± 0.011
6.241 ± 0.051
8.244 ± 0.073
4.461 ± 0.044
7.406 ± 0.05
2.313 ± 0.028
7.688 ± 0.053
5.739 ± 0.051
9.258 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.134 ± 0.024
4.139 ± 0.033
3.502 ± 0.026
2.851 ± 0.027
4.426 ± 0.042
5.762 ± 0.03
5.44 ± 0.027
7.177 ± 0.036
0.804 ± 0.015
3.592 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here