Beihai tombus-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFF1|A0A1L3KFF1_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 12 OX=1922715 PE=4 SV=1
MM1 pKa = 7.56AGRR4 pKa = 11.84RR5 pKa = 11.84GRR7 pKa = 11.84QRR9 pKa = 11.84GATRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84GNALASAQRR24 pKa = 11.84VLHH27 pKa = 5.6RR28 pKa = 11.84TSIPFDD34 pKa = 3.4VADD37 pKa = 3.96ATGASCTKK45 pKa = 10.24IVLDD49 pKa = 3.96PSAYY53 pKa = 8.99TKK55 pKa = 10.91DD56 pKa = 3.42RR57 pKa = 11.84ASAMASIFQKK67 pKa = 10.06YY68 pKa = 8.38AVRR71 pKa = 11.84SVAVDD76 pKa = 3.86FTPSVGFNEE85 pKa = 5.54SSGQWGVGYY94 pKa = 8.55MADD97 pKa = 4.68EE98 pKa = 4.63SNQGQGLPSTTADD111 pKa = 3.28VVAMARR117 pKa = 11.84QGCGTLSPVRR127 pKa = 11.84SKK129 pKa = 10.72LHH131 pKa = 4.95WAVPRR136 pKa = 11.84RR137 pKa = 11.84SLRR140 pKa = 11.84NITEE144 pKa = 4.78GYY146 pKa = 7.72TPGGAEE152 pKa = 3.56QPGAVFVGVAAAASVAQPGVWTLDD176 pKa = 2.94ILYY179 pKa = 8.4EE180 pKa = 4.16FWGPRR185 pKa = 11.84PPFEE189 pKa = 4.24KK190 pKa = 10.5AVLPTEE196 pKa = 4.05ITSGGGYY203 pKa = 10.44GITFGDD209 pKa = 4.27GSDD212 pKa = 3.21WPYY215 pKa = 11.24GYY217 pKa = 10.37HH218 pKa = 6.98PPDD221 pKa = 3.89ALWRR225 pKa = 11.84NLWRR229 pKa = 11.84ARR231 pKa = 11.84DD232 pKa = 3.8SCSLTTVTSFYY243 pKa = 10.71AHH245 pKa = 6.61TSAGVFLLEE254 pKa = 4.39GPGKK258 pKa = 9.8IEE260 pKa = 3.73QLEE263 pKa = 4.14GLGTVEE269 pKa = 4.76VINAFMDD276 pKa = 3.54THH278 pKa = 6.94SLLGVSLFGTNAMKK292 pKa = 10.21QIPIPAVRR300 pKa = 11.84QAVSVQPEE308 pKa = 3.64FRR310 pKa = 11.84VRR312 pKa = 11.84VINEE316 pKa = 3.39VDD318 pKa = 3.93DD319 pKa = 4.22PVPTQEE325 pKa = 4.61TVDD328 pKa = 3.95PLPVEE333 pKa = 4.47VTNVPLPVAEE343 pKa = 4.48TVDD346 pKa = 3.91PLPVEE351 pKa = 4.47VTNVPLPVAEE361 pKa = 4.48TVDD364 pKa = 3.91PLPVEE369 pKa = 4.47VTNVPLPVAEE379 pKa = 4.48TVDD382 pKa = 3.91PLPVEE387 pKa = 4.47VTNVPLPVAEE397 pKa = 4.48TVDD400 pKa = 4.05PLPVDD405 pKa = 3.62VTNTPLPVAEE415 pKa = 4.68VVDD418 pKa = 4.19PLPVKK423 pKa = 9.29VTNTPLPVAEE433 pKa = 4.72AQLPLPVNEE442 pKa = 4.7TNLPLPVDD450 pKa = 4.1DD451 pKa = 4.89GGGGFFMNLATGVITAVVKK470 pKa = 10.15QAGRR474 pKa = 11.84TYY476 pKa = 10.56TRR478 pKa = 11.84DD479 pKa = 3.49SKK481 pKa = 9.82EE482 pKa = 4.0TPAGYY487 pKa = 10.36VRR489 pKa = 11.84LKK491 pKa = 10.45CSYY494 pKa = 9.34PVGGAVDD501 pKa = 3.93GQVYY505 pKa = 10.61GSVNIPAYY513 pKa = 7.1PTAEE517 pKa = 3.96AGLTHH522 pKa = 7.41FGRR525 pKa = 11.84LDD527 pKa = 3.14AGYY530 pKa = 10.07DD531 pKa = 3.6YY532 pKa = 11.42ASEE535 pKa = 4.59LFWKK539 pKa = 10.13RR540 pKa = 11.84FANMTVGLPNDD551 pKa = 3.14HH552 pKa = 7.06WYY554 pKa = 10.58KK555 pKa = 10.66AVSSEE560 pKa = 3.96PPPFGGAGGMYY571 pKa = 9.7IYY573 pKa = 9.66ITDD576 pKa = 3.47TRR578 pKa = 11.84KK579 pKa = 9.36PAGLFAGNHH588 pKa = 3.68ITARR592 pKa = 11.84CRR594 pKa = 11.84WTSAEE599 pKa = 3.86WFEE602 pKa = 4.0VHH604 pKa = 6.49CWHH607 pKa = 6.75NVDD610 pKa = 6.0SDD612 pKa = 4.16ASWSKK617 pKa = 10.9DD618 pKa = 3.2VHH620 pKa = 6.61CFLLHH625 pKa = 6.29KK626 pKa = 10.01PLYY629 pKa = 10.37DD630 pKa = 3.84SEE632 pKa = 5.99LDD634 pKa = 3.85DD635 pKa = 6.05VYY637 pKa = 11.21DD638 pKa = 3.88TYY640 pKa = 11.41QDD642 pKa = 3.21HH643 pKa = 6.92RR644 pKa = 11.84VRR646 pKa = 11.84THH648 pKa = 6.28PVV650 pKa = 2.58

Molecular weight: 70.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFE5|A0A1L3KFE5_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 12 OX=1922715 PE=4 SV=1
MM1 pKa = 7.42GAAQTVVRR9 pKa = 11.84RR10 pKa = 11.84DD11 pKa = 3.41YY12 pKa = 11.36AGVDD16 pKa = 4.01FGHH19 pKa = 7.7AARR22 pKa = 11.84STAVVPIVVIIGLVSVPLLIYY43 pKa = 10.3FRR45 pKa = 11.84SSGRR49 pKa = 11.84WCLGALQGLLRR60 pKa = 11.84SARR63 pKa = 11.84ASWLGSKK70 pKa = 8.68YY71 pKa = 9.64HH72 pKa = 5.74QRR74 pKa = 11.84LVVPARR80 pKa = 11.84IMFQDD85 pKa = 3.04AHH87 pKa = 7.27DD88 pKa = 4.46GVLDD92 pKa = 3.53VAARR96 pKa = 11.84KK97 pKa = 9.67LGGMIANRR105 pKa = 11.84ARR107 pKa = 11.84FYY109 pKa = 11.45EE110 pKa = 4.23EE111 pKa = 5.28LDD113 pKa = 3.58VPLLMIHH120 pKa = 5.43EE121 pKa = 4.46QVRR124 pKa = 3.52

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1977

124

650

395.4

43.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.521 ± 0.532

2.175 ± 0.523

4.148 ± 0.711

4.451 ± 0.514

2.833 ± 0.758

7.688 ± 0.683

1.973 ± 0.377

2.479 ± 0.483

3.794 ± 1.026

7.436 ± 1.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.498

2.681 ± 0.36

8.346 ± 1.148

3.44 ± 0.696

9.256 ± 1.624

6.677 ± 0.655

6.626 ± 1.261

8.7 ± 1.324

1.669 ± 0.273

3.035 ± 0.857
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski