Arthrobacter phage Sonali
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411CQI8|A0A411CQI8_9CAUD Uncharacterized protein OS=Arthrobacter phage Sonali OX=2510495 GN=72 PE=4 SV=1
MM1 pKa = 7.73 SIQSAPYY8 pKa = 8.5 YY9 pKa = 9.9 WYY11 pKa = 9.95 EE12 pKa = 4.26 CDD14 pKa = 4.28 QEE16 pKa = 4.3 EE17 pKa = 4.69 CGRR20 pKa = 11.84 KK21 pKa = 8.03 STEE24 pKa = 3.96 GSDD27 pKa = 3.28 YY28 pKa = 11.36 AAWSDD33 pKa = 4.32 KK34 pKa = 11.21 DD35 pKa = 4.14 GAWVEE40 pKa = 4.38 ADD42 pKa = 3.19 CSDD45 pKa = 3.66 WIEE48 pKa = 4.19 VEE50 pKa = 4.19 GKK52 pKa = 9.88 HH53 pKa = 5.81 YY54 pKa = 10.33 CHH56 pKa = 6.05 QHH58 pKa = 4.87 AHH60 pKa = 5.72 LHH62 pKa = 6.83 DD63 pKa = 5.91 PDD65 pKa = 4.08 TQDD68 pKa = 3.34 EE69 pKa = 4.57 EE70 pKa = 5.12 EE71 pKa = 4.71 EE72 pKa = 4.01 EE73 pKa = 4.66 SPTHH77 pKa = 6.65 KK78 pKa = 10.78 VV79 pKa = 2.95
Molecular weight: 9.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.093
IPC2_protein 4.291
IPC_protein 4.215
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.062
Grimsley 3.973
Solomon 4.164
Lehninger 4.126
Nozaki 4.291
DTASelect 4.444
Thurlkill 4.075
EMBOSS 4.075
Sillero 4.329
Patrickios 0.604
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.235
Protein with the highest isoelectric point:
>tr|A0A411CQR5|A0A411CQR5_9CAUD Major capsid protein OS=Arthrobacter phage Sonali OX=2510495 GN=7 PE=4 SV=1
MM1 pKa = 7.41 VITYY5 pKa = 9.88 KK6 pKa = 9.97 RR7 pKa = 11.84 TGTRR11 pKa = 11.84 TRR13 pKa = 11.84 VTRR16 pKa = 11.84 DD17 pKa = 2.88 GALLGWIDD25 pKa = 3.75 RR26 pKa = 11.84 RR27 pKa = 11.84 LGSDD31 pKa = 3.35 SSPRR35 pKa = 11.84 GGAYY39 pKa = 8.2 RR40 pKa = 11.84 TFHH43 pKa = 6.89 HH44 pKa = 7.51 PITPTGEE51 pKa = 3.86 RR52 pKa = 11.84 LAPCWSRR59 pKa = 11.84 KK60 pKa = 7.57 EE61 pKa = 3.98 AVEE64 pKa = 4.05 DD65 pKa = 4.11 LARR68 pKa = 11.84 QADD71 pKa = 3.99 KK72 pKa = 11.54 NN73 pKa = 3.84
Molecular weight: 8.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.56
IPC_protein 10.526
Toseland 10.613
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.774
Solomon 10.862
Lehninger 10.833
Nozaki 10.599
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.862
IPC2_peptide 9.575
IPC2.peptide.svr19 8.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
0
99
19167
49
1537
193.6
20.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.257 ± 0.753
0.757 ± 0.107
5.703 ± 0.32
5.796 ± 0.437
2.755 ± 0.138
8.165 ± 0.326
2.03 ± 0.221
4.341 ± 0.168
4.221 ± 0.326
8.029 ± 0.246
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.056 ± 0.116
3.177 ± 0.187
6.104 ± 0.402
3.913 ± 0.19
5.656 ± 0.328
5.603 ± 0.255
7.096 ± 0.353
7.346 ± 0.228
1.675 ± 0.141
2.322 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here