Streptomyces phage RosaAsantewaa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Scapunavirus; unclassified Scapunavirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E5S6|A0A4D6E5S6_9CAUD Portal protein OS=Streptomyces phage RosaAsantewaa OX=2562347 GN=8 PE=4 SV=1
MM1 pKa = 7.18MNLYY5 pKa = 10.41EE6 pKa = 4.13DD7 pKa = 3.65TVYY10 pKa = 11.3NGLLAVFWGLRR21 pKa = 11.84EE22 pKa = 4.09EE23 pKa = 4.18VLAWLEE29 pKa = 4.17DD30 pKa = 3.81NPEE33 pKa = 3.8IAYY36 pKa = 10.04GGYY39 pKa = 9.66QVFDD43 pKa = 3.33MRR45 pKa = 11.84AGRR48 pKa = 11.84DD49 pKa = 3.38KK50 pKa = 11.14TVIEE54 pKa = 4.74YY55 pKa = 10.3ISAAA59 pKa = 3.54

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E4N7|A0A4D6E4N7_9CAUD Uncharacterized protein OS=Streptomyces phage RosaAsantewaa OX=2562347 GN=49 PE=4 SV=1
MM1 pKa = 7.28NDD3 pKa = 2.37VWNFYY8 pKa = 9.06IANWLLVFYY17 pKa = 10.12IIIGSALAAIGVTKK31 pKa = 10.87VIDD34 pKa = 3.87TVIEE38 pKa = 3.74RR39 pKa = 11.84AKK41 pKa = 9.89RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84YY45 pKa = 9.71RR46 pKa = 11.84KK47 pKa = 9.88ARR49 pKa = 11.84TT50 pKa = 3.24

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13434

39

1739

244.3

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.238 ± 0.742

0.432 ± 0.099

6.216 ± 0.319

6.811 ± 0.467

3.729 ± 0.274

7.481 ± 0.387

1.466 ± 0.19

5.211 ± 0.276

6.402 ± 0.412

8.173 ± 0.539

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.126

4.347 ± 0.146

3.99 ± 0.319

3.29 ± 0.167

4.972 ± 0.412

6.067 ± 0.263

7.049 ± 0.436

7.503 ± 0.281

1.377 ± 0.188

3.603 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski